Bacteremia caused by Janibacter melonis.

abstract：:HPV68a is not efficiently detected by PCR with the PGMY primers. Version 2 of the PGMY-CHUV assay (PGv2) was developed from version 1 (PGv1) to evaluate HPV68-discordant results with the Anyplex II HPV28 assay. We now report that PGv2 is significantly more sensitive than PGv1 for HPV68a and as sensitive and specific f...

journal_title：Journal of clinical microbiology

authors： Estrade C,Sahli R

更新日期：2014-11-01 00:00:00

abstract：:We compared the BD Phoenix automated microbiology system to the Bruker Biotyper (version 2.0) matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) system for identification of gram-negative bacilli, using biochemical testing and/or genetic sequencing to resolve discordant resul...

journal_title：Journal of clinical microbiology

authors： Saffert RT,Cunningham SA,Ihde SM,Jobe KE,Mandrekar J,Patel R

更新日期：2011-03-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been found to be an accurate, rapid, and inexpensive method for the identification of bacteria and yeasts. Previous evaluations have compared the accuracy, time to identification, and costs of the MALDI-TOF MS method agains...

journal_title：Journal of clinical microbiology

authors： Tan KE,Ellis BC,Lee R,Stamper PD,Zhang SX,Carroll KC

更新日期：2012-10-01 00:00:00

abstract：:The present report describes an analysis of two virulence genes of Helicobacter pylori. Parts of the cagA gene, as well as parts from the signal (s) and middle (m) regions of the mosaic vacA gene, were amplified with biotin-labelled PCR primers and the products were subsequently analyzed by a single-step reverse hybri...

journal_title：Journal of clinical microbiology

authors： van Doorn LJ,Figueiredo C,Rossau R,Jannes G,van Asbroek M,Sousa JC,Carneiro F,Quint WG

更新日期：1998-05-01 00:00:00

abstract：:Of 532 strains of viridans streptococci isolated from clinical specimens, 517 were identified by using a scheme based on the work of Facklam (R. R. Facklam, J. Clin. Microbiol. 5:184-201, 1977). The strains were distributed among nine of the species described by Facklam and an additional group of physiologically homog...

journal_title：Journal of clinical microbiology

authors： Ruoff KL,Kunz LJ

更新日期：1982-05-01 00:00:00

abstract：:We performed pulsed-field gel electrophoresis on Escherichia coli O157 isolates (n = 318) from 199 healthy animals in a longitudinal study carried out on nine farms. Investigation of the restriction types proved that at the farm level, the same clones can be detected on sampling occasions separated by as much as 17 mo...

journal_title：Journal of clinical microbiology

authors： Liebana E,Smith RP,Batchelor M,McLaren I,Cassar C,Clifton-Hadley FA,Paiba GA

更新日期：2005-02-01 00:00:00

abstract：:We report a failure of the real-time reverse transcriptase PCR H7 subtyping protocol currently used in national avian influenza surveillance programs. Significant substitutions in primer and probe target sequences were identified, especially in wild bird viruses. The protocol, originally designed for detecting H7 infl...

journal_title：Journal of clinical microbiology

authors： Xing Z,Cardona C,Dao P,Crossley B,Hietala S,Boyce W

更新日期：2008-05-01 00:00:00

abstract：:Cryptococcus neoformans var. neoformans (serotype D) and C. neoformans var. grubii (serotype A) differ in geographic prevalence and dermatotropism, with C. neoformans var. neoformans strains being more prevalent among isolates from temperate countries as well as from skin infections. Analysis of 19 strains from each s...

journal_title：Journal of clinical microbiology

authors： Martinez LR,Garcia-Rivera J,Casadevall A

更新日期：2001-09-01 00:00:00

abstract：:A 13-year-old patient developed severe shock due to administration of a Yersinia enterocolitica-contaminated red blood cell concentrate. Y. enterocolitica (serotype O:9, biotype II) was cultivated from the residual blood in the blood bag and from a stool sample of the blood donor. In the donor's plasma immunoglobulin ...

journal_title：Journal of clinical microbiology

authors： Strobel E,Heesemann J,Mayer G,Peters J,Müller-Weihrich S,Emmerling P

更新日期：2000-07-01 00:00:00

abstract：:Superparamagnetic polymer particles precoated with sheep anti-mouse immunoglobulin G were coated with immunoglobulin G2 monoclonal antibodies to the K88 antigen of Escherichia coli (MAb-K88). These immunomagnetic particles (IMP) were used for isolation and identification of K88 antigen-positive (K88+) E. coli. The bac...

journal_title：Journal of clinical microbiology

authors： Lund A,Hellemann AL,Vartdal F

更新日期：1988-12-01 00:00:00

abstract：:During the 1971 epidemic of Venezuelan equine encephalomyelitis (VEE) in south Texas, 203 suspect VEE cases were evaluated by the Center for Disease Control. Sixty-seven were confirmed as cases of VEE. Laboratory confirmation was accomplished by isolation of VEE virus from a serum specimen taken during the acute illne...

journal_title：Journal of clinical microbiology

authors： Bowen GS,Calisher CH

更新日期：1976-07-01 00:00:00

abstract：:A replicator method for screening Staphylococcus aureus isolates for synergistic hemolytic activity is described. The technique should also be useful for assessing the hemolytic activity of staphylococci and streptococci on different lots or brands of blood agar because the inoculum and the distances between spot inoc...

journal_title：Journal of clinical microbiology

authors： Boyce JM

更新日期：1985-05-01 00:00:00

abstract：:A passive hemagglutination (PHA) assay for antibody to Pseudomonas aeruginosa exotoxin is described which utilizes chromic chloride-treated ovine erythrocytes coated with purified toxin. PHA antitoxin titers correlated well with those obtained by a cytotoxicity neutralization assay (r = 0.91, P less than 0.001), where...

journal_title：Journal of clinical microbiology

authors： Pollack M,Taylor NS

更新日期：1977-07-01 00:00:00

abstract：:The Isolator 10 lysis-centrifugation blood culture system (E. I. du Pont de Nemours & Co., Inc., Wilmington, Del.) was compared with the BACTEC radiometric method (Johnston Laboratories, Inc., Towson, Md.) with 6B and 7D broth media for the recovery of bacteria and yeasts. From 11,000 blood cultures, 1,174 clinically ...

journal_title：Journal of clinical microbiology

authors： Brannon P,Kiehn TE

更新日期：1985-12-01 00:00:00

abstract：:The IS200-like insertion sequence (IS) is a 708-bp element recently found in Yersinia pestis. Its nucleotide sequence is 85% identical to that of IS200 recovered in most Salmonella enterica isolates. It is also present in multiple copies in Y. pseudotuberculosis. In contrast, this IS is found in some (biotype 1B strai...

journal_title：Journal of clinical microbiology

authors： Odaert M,Berche P,Simonet M

更新日期：1996-09-01 00:00:00

abstract：:Between 1998 and 2001, tissues from four captive white-tailed deer were observed to have histologic lesions of systemic lymphocytic vasculitis. These lesions suggested malignant catarrhal fever, although epizootic hemorrhagic disease and bluetongue were included in the differential diagnosis. Initial diagnostic effort...

journal_title：Journal of clinical microbiology

authors： Kleiboeker SB,Miller MA,Schommer SK,Ramos-Vara JA,Boucher M,Turnquist SE

更新日期：2002-04-01 00:00:00

abstract：:A simple, rapid procedure to separate Pneumocystis carinii obtained by lavage of lungs of steroid-treated rats from rat leukocytes is described. Commercially available monoclonal antibody to rat common leukocyte determinants is used to sediment the leukocytes, resulting in supernatant fluid containing P. carinii cells...

journal_title：Journal of clinical microbiology

authors： Pesanti EL,McCarron BA

更新日期：1988-08-01 00:00:00

abstract：:Using the Staphylococcus aureus absorption method, we have modified a commercial radioimmunoassay kit for the determination of total antibody to hepatitis B core antigen so that we can now determine the predominant globulin species of that antibody. One-hundred percent of acute hepatitis B sera had a predominance of i...

journal_title：Journal of clinical microbiology

authors： Schable CA,Maynard JE

更新日期：1982-11-01 00:00:00

abstract：:Fifty-eight fusaria isolated from 50 Italian patients between 2004 and 2007 were subject to multilocus DNA sequence typing to characterize the spectrum of species and circulating sequence types (STs) associated with dermatological infections, especially onychomycoses and paronychia, and other fusarioses in northern an...

journal_title：Journal of clinical microbiology

authors： Migheli Q,Balmas V,Harak H,Sanna S,Scherm B,Aoki T,O'Donnell K

更新日期：2010-04-01 00:00:00

abstract：:Testing for susceptibility to pyrazinamide (PZA) and analysis of the pncA gene sequences of 423 Mycobacterium tuberculosis complex isolates have revealed a unique silent nucleotide substitution that enables the rapid identification of "M. canettii" (proposed name). Moreover, the lack of a defined mutation within the p...

journal_title：Journal of clinical microbiology

authors： Somoskovi A,Dormandy J,Parsons LM,Kaswa M,Goh KS,Rastogi N,Salfinger M

更新日期：2007-02-01 00:00:00

abstract：:We describe the adoption of nucleic acid amplification tests (NAAT) for Clostridium difficile diagnosis and their impact on stool rejection policies and C. difficile positivity rates. Of the laboratories with complete surveys, 51 (43%) reported using NAAT in 2011. Laboratories using NAAT had stricter rejection policie...

journal_title：Journal of clinical microbiology

authors： Cohen J,Limbago B,Dumyati G,Holzbauer S,Johnston H,Perlmutter R,Dunn J,Nadle J,Lyons C,Phipps E,Beldavs Z,Clark LA,Lessa FC,CDC's Clostridium difficile Infection Surveillance Investigators.

更新日期：2014-02-01 00:00:00

abstract：:A total of 244 strains of Aerococcus, Enterococcus, and Streptococcus species were tested by the RapID STR system (Innovative Diagnostic Systems Inc., Atlanta, Ga.) for identification. Strains were identified without additional tests or with additional conventional tests suggested by the IDS compendium manual. Our dat...

journal_title：Journal of clinical microbiology

authors： You MS,Facklam RR

更新日期：1986-10-01 00:00:00

abstract：:Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral zoonosis which occurs throughout Africa, Eastern Europe, and Asia and results in an approximately 30% fatality rate. A reverse transcription-PCR assay including a competitive internal control was developed on the basis of the most up-to-date genome informati...

journal_title：Journal of clinical microbiology

authors： Wölfel R,Paweska JT,Petersen N,Grobbelaar AA,Leman PA,Hewson R,Georges-Courbot MC,Papa A,Heiser V,Panning M,Günther S,Drosten C

更新日期：2009-04-01 00:00:00

abstract：:Real-time PCR with melting curve analysis of PCR products is a rapid procedure for detecting and genotyping herpes simplex virus (HSV). When testing mucocutaneous samples for HSV by a real-time PCR assay targeting the DNA polymerase gene, we found that some PCR products had atypical melting curves that did not conform...

journal_title：Journal of clinical microbiology

authors： Anderson TP,Werno AM,Beynon KA,Murdoch DR

更新日期：2003-05-01 00:00:00

abstract：:Urinary tract infections caused by staphylococci are usually attributed to Staphylococcus epidermidis or S. saprophyticus. The case study reported here describes a persistent urinary tract infection caused by S. haemolyticus in a 38-year-old male whose infection was ultimately resolved through the use of the antibioti...

journal_title：Journal of clinical microbiology

authors： Gunn BA,Davis CE Jr

更新日期：1988-05-01 00:00:00

abstract：:To determine the excretion dynamics and genotypic characteristics of rotavirus A (RVA), a longitudinal observational study was performed in 10 pigs from 3 litters at a farrow-to-finish farm. A total of 400 fecal samples were directly collected from the rectums of individual pigs (aged 7 to 217 days) at 3- to 14-day in...

journal_title：Journal of clinical microbiology

authors： Miyazaki A,Kuga K,Suzuki T,Tsunemitsu H

更新日期：2012-06-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of immunoglobulin G (IgG) to the capsular polysaccharide (CP) of Pasteurella haemolytica serotype 1. Purified CP was first covalently coupled to poly-L-lysine and then optimally adsorbed at a concentration of 5 micrograms/ml to microtiter pla...

journal_title：Journal of clinical microbiology

authors： Townsend EL,Maheswaran SK,Leininger JR,Ames TR

更新日期：1987-02-01 00:00:00

abstract：:Whole-genome sequencing (WGS) of Staphylococcus aureus is increasingly used as part of infection prevention practices. In this study, we established a long-read technology-based WGS screening program of all first-episode methicillin-resistant Staphylococcus aureus (MRSA) blood infections at a major urban hospital. A s...

journal_title：Journal of clinical microbiology

authors： Sullivan MJ,Altman DR,Chacko KI,Ciferri B,Webster E,Pak TR,Deikus G,Lewis-Sandari M,Khan Z,Beckford C,Rendo A,Samaroo F,Sebra R,Karam-Howlin R,Dingle T,Hamula C,Bashir A,Schadt E,Patel G,Wallach F,Kasarskis A,Gi

更新日期：2019-11-22 00:00:00

abstract：:Campylobacter jejuni (20 strains) and Campylobacter coli (12 strains) were assigned to four biovars for each species based on phenotypic tests that were easy to perform and interpret. The resulting biotyping schemes offer a greater degree of distinction among C. jejuni and C. coli strains than any of the other biotypi...

journal_title：Journal of clinical microbiology

authors： Roop RM 2nd,Smibert RM,Krieg NR

更新日期：1984-11-01 00:00:00

abstract：:We cloned and characterized a phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) gene from Burkholderia pseudomallei. DNA sequence analysis of the gene indicated an open reading frame coding for 700 amino acids with a 34-amino-acid signal peptide. When cleaved, this yields a secreted 73-kDa mature protein. The d...

journal_title：Journal of clinical microbiology

authors： Korbsrisate S,Suwanasai N,Leelaporn A,Ezaki T,Kawamura Y,Sarasombath S

更新日期：1999-11-01 00:00:00