Abstract:
:The lack of a quick, simple, and inexpensive diagnostic test has limited the ability of public health officials to rapidly assess and control outbreaks of Giardia lamblia in child day-care centers. We evaluated the performance of a commercially available enzyme-linked immunosorbent assay (ELISA) for the detection of a G. lamblia-associated antigen in stool. Stool specimens were collected from the diapers of 426 children attending 20 day-care centers, fixed in 10% Formalin and polyvinyl alcohol, and examined by microscopy by Formalin concentration and trichrome staining techniques. Specimens were also tested visually and spectrophotometrically by ELISA. Of 99 tests positive by microscopy, 93 were visually positive by ELISA (sensitivity, 93.9%). Of 534 tests negative for G. lamblia by microscopy, 32 (6.0%) were ELISA positive. However, on the basis of examination of multiple specimens from the same child, none of these could be considered false-positive ELISAs; the specificity of the ELISA was therefore 100%. The sensitivity of both microscopy and ELISA improved as the number of specimens per child increased. An optical density value of greater than 0.040 was 98.0% sensitive and 100% specific for G. lamblia. This ELISA, which appeared to be more sensitive for G. lamblia than did microscopic examination of stool, should be useful as an epidemiologic tool, particularly in day-care settings, and may also have a role in confirming clinical diagnoses of giardiasis.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Addiss DG,Mathews HM,Stewart JM,Wahlquist SP,Williams RM,Finton RJ,Spencer HC,Juranek DDdoi
10.1128/JCM.29.6.1137-1142.1991subject
Has Abstractpub_date
1991-06-01 00:00:00pages
1137-42issue
6eissn
0095-1137issn
1098-660Xjournal_volume
29pub_type
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.24.4.677-679.1986
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.32.5.1346-1348.1994
更新日期:1994-05-01 00:00:00
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journal_title:Journal of clinical microbiology
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