High percentage of methicillin-resistant Staphylococcus aureus isolates with reduced susceptibility to glycopeptides in The Netherlands.


:While testing the in vitro activities of 14 antimicrobial agents against 107 methicillin-susceptible Staphylococcus aureus (MSSA) and 250 methicillin-resistant S. aureus (MRSA) isolates collected in The Netherlands, we found to our surprise that 19 (7.6%) MRSA isolates were suspected of having reduced susceptibilities to the glycopeptides when the Etest system (AB Biodisk, Solna, Sweden) was used with a large inoculum (no. 2 McFarland standard) and an extended incubation time (48 h) on brain heart infusion agar for MIC testing. Eventually, 15 of these isolates were classified as heterogeneously resistant to glycopeptides (heterogeneously glycopeptide-intermediate S. aureus [hGISA] isolates) according to the population analysis profile-area under the curve analysis. The MICs at which 50 and 90% of isolates are inhibited obtained with the Etest system with the large inoculum were as follows: for MSSA isolates, 3.0 and 4.0 micro g/ml, respectively, for both teicoplanin and vancomycin; for MRSA isolates, 3.0 and 8.0 micro g/ml, respectively, for teicoplanin, and 3.0 and 4.0 micro g/ml, respectively, for vancomycin. This is the first report of hGISA isolates in The Netherlands.


J Clin Microbiol


Van Griethuysen A,Van 't Veen A,Buiting A,Walsh T,Kluytmans J




Has Abstract


2003-06-01 00:00:00












  • Comparison and evaluation of antimicrobial susceptibility testing of enterococci performed in accordance with six national committee standardized disk diffusion procedures.

    abstract::Studies were conducted to compare and evaluate antimicrobial susceptibility test results for enterococci obtained by six national committee disk diffusion procedures. Variations in the incidence of isolates in resistance categories and errors were associated with the use of ciprofloxacin, gentamicin, nitrofurantoin, r...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Cotter G,Adley CC

    更新日期:2001-10-01 00:00:00

  • Detection by GenoType MTBDRsl test of complex mechanisms of resistance to second-line drugs and ethambutol in multidrug-resistant Mycobacterium tuberculosis complex isolates.

    abstract::The GenoType MTBDRsl test rapidly detects resistance to ethambutol, fluoroquinolones, and second-line aminoglycosides (amikacin and kanamycin) and cyclic peptide (capreomycin) in Mycobacterium tuberculosis. A set of 41 multidrug-resistant (MDR) M. tuberculosis strains, 8 extensively drug-resistant (XDR) M. tuberculosi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Brossier F,Veziris N,Aubry A,Jarlier V,Sougakoff W

    更新日期:2010-05-01 00:00:00

  • Campylobacter hyointestinalis: an opportunistic enteropathogen?

    abstract::A new case of Campylobacter hyointestinalis-associated diarrhea in a human is reported. The medical history of the patient was significant for immunodeficiency because of an evolutive chronic myeloid leukemia. The diarrhea rapidly stopped after administration of oral erythromycin. No other enteropathogenic agent was f...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Minet J,Grosbois B,Megraud F

    更新日期:1988-12-01 00:00:00

  • Isolation of a strain-specific Entamoeba histolytica cDNA clone.

    abstract::Entamoeba histolytica is an intestinal parasite causing significant morbidity and mortality worldwide. More tools are needed to understand the epidemiology and molecular pathogenesis of amebiasis. A cDNA library was constructed by using poly(A)+ RNA isolated from an axenic strain of E. histolytica, HM1:IMSS, which exp...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Burch DJ,Li E,Reed S,Jackson TF,Stanley SL Jr

    更新日期:1991-04-01 00:00:00

  • Molecular Epidemiology of Streptococcus uberis Clinical Mastitis in Dairy Herds: Strain Heterogeneity and Transmission.

    abstract::Multilocus sequence typing was successfully completed on 494 isolates of Streptococcus uberis from clinical mastitis cases in a study of 52 commercial dairy herds over a 12-month period. In total, 195 sequence types (STs) were identified. S. uberis mastitis cases that occurred in different cows within the same herd an...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Davies PL,Leigh JA,Bradley AJ,Archer SC,Emes RD,Green MJ

    更新日期:2016-01-01 00:00:00

  • Survey of extended-spectrum beta-lactamases in clinical isolates of Escherichia coli and Klebsiella pneumoniae: prevalence of TEM-52 in Korea.

    abstract::Two hundred ninety isolates of Escherichia coli were investigated for the production of extended-spectrum beta-lactamases (ESBLs). Fourteen (4.8%) of the 290 strains were found to produce ESBLs. Each of the 14 strains produced one or two ESBLs, as follows: 10 strains produced TEM-52, 1 strain produced SHV-2a, 1 strain...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Pai H,Lyu S,Lee JH,Kim J,Kwon Y,Kim JW,Choe KW

    更新日期:1999-06-01 00:00:00

  • Evaluation of the NG-Test MCR-1 Lateral Flow Assay and EDTA-Colistin Broth Disk Elution Methods To Detect Plasmid-Mediated Colistin Resistance among Gram-Negative Bacterial Isolates.

    abstract::Plasmid-mediated colistin resistance (PMCR) is a global public health concern, given its ease of transmissibility. The purpose of this study was to evaluate two methods for the detection of PMCR from bacterial colonies: (i) the NG-Test MCR-1 lateral flow immunoassay (LFA; NG Biotech, Guipry, France) and (ii) the EDTA-...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Fenwick AJ,Bergman Y,Lewis S,Yee R,Uhlemann AC,Cole N,Kohner P,Ordak C,Green DA,Schuetz AN,Humphries R,Simner PJ

    更新日期:2020-03-25 00:00:00

  • Identification of Pandoraea species by 16S ribosomal DNA-based PCR assays.

    abstract::The recently described genus Pandoraea contains five named species (Pandoraea apista, Pandoraea pulmonicola, Pandoraea pnomenusa, Pandoraea sputorum, and Pandoraea norimbergensis) and four unnamed genomospecies. Pandoraea spp. have mainly been recovered from the respiratory tracts of cystic fibrosis (CF) patients. Acc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Coenye T,Liu L,Vandamme P,LiPuma JJ

    更新日期:2001-12-01 00:00:00

  • Evaluation of GenoType mycobacteria direct assay in comparison with Gen-Probe Mycobacterium tuberculosis amplified direct test and GenoType MTBDRplus for direct detection of Mycobacterium tuberculosis complex in clinical samples.

    abstract::Three molecular assays were evaluated for the direct detection of Mycobacterium tuberculosis complex bacteria in 125 respiratory and 22 nonrespiratory samples. The overall sensitivities obtained were as follows: GenoType MTBDRplus, 97.9%; GenoType Mycobacteria Direct, 93.7%; Gen-Probe Mycobacterium tuberculosis Amplif...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Neonakis IK,Gitti Z,Baritaki S,Petinaki E,Baritaki M,Spandidos DA

    更新日期:2009-08-01 00:00:00

  • The Capnocytophaga canimorsus isolate that caused sepsis in an immunosufficient man was transmitted by the large pine weevil Hylobius abietis.

    abstract::The first vector-borne Capnocytophaga canimorsus sepsis case is presented. An immunocompetent male who denied any contact with canines and who worked in a sawmill was bitten on his neck by a large pine weevil (Hylobius abietis L.; Coleoptera: Curculionidae). Bacteriological diagnosis was confirmed by 16S rRNA gene seq...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Tuuminen T,Viiri H,Vuorinen S

    更新日期:2014-07-01 00:00:00

  • Molecular typing by random amplification of polymorphic DNA and M13 southern hybridization of related paired isolates of Aspergillus fumigatus.

    abstract::Three forms of DNA-based typing procedures for Aspergillus fumigatus isolates have been developed over the last five years. The procedures are random amplification of polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) detection, and Southern hybridizations with various repetitive sequence-based pr...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Anderson MJ,Gull K,Denning DW

    更新日期:1996-01-01 00:00:00

  • Simultaneous amplification and identification of 25 human papillomavirus types with Templex technology.

    abstract::The majority of existing human papillomavirus (HPV) genotyping assays are based on multiplex PCR using consensus or degenerate primers. We developed a Templex HPV assay that simultaneously detects and identifies 25 common HPV genotypes in a single-tube reaction using type-specific primers for the HPV-specific E6 and E...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Han J,Swan DC,Smith SJ,Lum SH,Sefers SE,Unger ER,Tang YW

    更新日期:2006-11-01 00:00:00

  • Analysis of Helicobacter pylori genotypes and correlation with clinical outcome in Turkey.

    abstract::The predominant Helicobacter pylori strains circulating among geographic locations differ in regard to genomic structure. The association of the cagA-positive, vacA s1 genotypes with peptic ulcer disease (PUD) and gastric cancer was reported in Western countries but not in East Asian countries. Strains from Western co...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Saribasak H,Salih BA,Yamaoka Y,Sander E

    更新日期:2004-04-01 00:00:00

  • Nonclinical selection criteria for maximizing yield of nucleic acid amplification tests in tuberculosis diagnosis.

    abstract::In spite of the excellent performance of rapid tuberculosis (TB) nucleic acid amplification (NAA) tests and the clear benefits of immediate diagnosis of TB disease, NAA tests frequently are not used in the diagnosis of pulmonary TB cases, particularly TB cases with smear-negative sputa. Public health laboratories prim...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Han LL,Elvin P,Bernardo J

    更新日期:2012-08-01 00:00:00

  • Clonal reproduction and limited dispersal in an environmental population of Cryptococcus neoformans var gattii isolates from Australia.

    abstract::Cryptococcus neoformans var. gattii is a causative agent of cryptococcosis and is thought to have a specific ecological association with a number of Eucalyptus species in Australia. However, the role that the tree plays in the life cycle of the fungus and the nature of the infectious propagule are not well understood....

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Halliday CL,Carter DA

    更新日期:2003-02-01 00:00:00

  • Extensive horizontal gene transfer in ureaplasmas from humans questions the utility of serotyping for diagnostic purposes.

    abstract::Ureaplasma parvum and Ureaplasma urealyticum are sexually transmitted, opportunistic pathogens of the human urogenital tract. There are 14 known serovars distributed between the two species. For decades, it has been postulated based upon limited data that virulence is related to serotype specificity. The results were ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Xiao L,Paralanov V,Glass JI,Duffy LB,Robertson JA,Cassell GH,Chen Y,Waites KB

    更新日期:2011-08-01 00:00:00

  • Molecular epidemiology of Legionella species by restriction endonuclease and alloenzyme analysis.

    abstract::As part of an ongoing investigation into nosocomial Legionella infections at Stanford University Medical Center (SUMC), we applied the technique of restriction endonuclease analysis (REA) to determine strain differences among three species, including Legionella pneumophila, Legionella dumoffii, and Legionella micdadei...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Tompkins LS,Troup NJ,Woods T,Bibb W,McKinney RM

    更新日期:1987-10-01 00:00:00

  • Computer-assisted surveillance for detecting clonal outbreaks of nosocomial infection.

    abstract::Whole-house surveillance for healthcare-associated infection is no longer the recommended practice because of the large personnel time investment required. We developed a computer-based tracking system using microbiologic data as an aid in detecting potential outbreaks of healthcare-associated infections on a hospital...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Hacek DM,Cordell RL,Noskin GA,Peterson LR

    更新日期:2004-03-01 00:00:00

  • Antifungal drug susceptibility and phylogenetic diversity among Cryptococcus isolates from dogs and cats in North America.

    abstract::Molecular types of the Cryptococcus neoformans/Cryptococcus gattii species complex that infect dogs and cats differ regionally and with host species. Antifungal drug susceptibility can vary with molecular type, but the susceptibility of Cryptococcus isolates from dogs and cats is largely unknown. Cryptococcus isolates...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Singer LM,Meyer W,Firacative C,Thompson GR 3rd,Samitz E,Sykes JE

    更新日期:2014-06-01 00:00:00

  • Interlaboratory Comparison of Six Real-Time PCR Assays for Detection of Bovine Leukemia Virus Proviral DNA.

    abstract::Quantitative real-time PCR (qPCR) is increasingly being used for the detection of bovine leukemia virus (BLV) proviral DNA. Nevertheless, quality control for the validation and standardization of such tests is currently lacking. Therefore, the present study was initiated by three Office International des Epizooties (O...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Jaworski JP,Pluta A,Rola-Łuszczak M,McGowan SL,Finnegan C,Heenemann K,Carignano HA,Alvarez I,Murakami K,Willems L,Vahlenkamp TW,Trono KG,Choudhury B,Kuźmak J

    更新日期:2018-06-25 00:00:00

  • Antigenic characteristics of Borrelia burgdorferi isolates from ixodid ticks in California.

    abstract::Twenty (1.4%) of 1,421 adult Ixodes pacificus ticks and 2 (20%) of 10 adult Ixodes neotomae ticks collected in five counties of northern California were found to contain spirochetes by direct immunofluorescence examination of their tissues with a polyvalent conjugate. Borreliae isolated from the tissues of nine of the...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lane RS,Pascocello JA

    更新日期:1989-10-01 00:00:00

  • Molecular Subtyping of Human Rhinovirus in Children from Three Sub-Saharan African Countries.

    abstract::The pathogenesis of human rhinovirus (HRV) during severe respiratory disease remains undefined; thus, we aimed to explore the relationship between the HRV molecular subtyping results obtained during severe and asymptomatic childhood infections. Nasopharyngeal/oropharyngeal swabs from children (1 to 59 months of age) h...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Baillie VL,Moore DP,Mathunjwa A,Morailane P,Simões EAF,Madhi SA

    更新日期:2019-08-26 00:00:00

  • Life-threatening respiratory tract disease with human bocavirus-1 infection in a 4-year-old child.

    abstract::The disease spectrum associated with human bocavirus-1 infection remains to be fully defined. We report a case of bocavirus-1-associated bronchiolitis, leading to severe respiratory failure and extracorporeal membrane oxygenation in a 4-year-old child, and suggest blood testing for human bocavirus-1 in children with s...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Edner N,Castillo-Rodas P,Falk L,Hedman K,Söderlund-Venermo M,Allander T

    更新日期:2012-02-01 00:00:00

  • Phaeohyphomycosis caused by Alternaria infectoria in a renal transplant recipient.

    abstract::We report on a case of phaeohyphomycosis caused by Alternaria infectoria in a renal transplant recipient with pulmonary infiltrates and multiple skin lesions. Diagnosis was based on microscopy and culture of the skin lesions. Treatment consisted of a combination of surgical excision and systemic antifungal therapy, fi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Halaby T,Boots H,Vermeulen A,van der Ven A,Beguin H,van Hooff H,Jacobs J

    更新日期:2001-05-01 00:00:00

  • Performance characteristics of a commercially prepared biphasic blood culture bottle.

    abstract::A biphasic blood culture bottle (BiPB: GIBCO Laboratories, North Andover, Mass.) with an architectural design that physically separates the agar slant from the broth was compared with a conventional vented monophasic bottle (MPB-A) for use in the routine culture of blood. Both bottles contained tryptic soy broth. Tryp...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Weckbach LS,Staneck JL

    更新日期:1986-04-01 00:00:00

  • Serotyping of Serratia marcescens: current status of seven recently described flagellar (H) antigens.

    abstract::The slightly revised, current scheme of 20 flagellar (H) antigens of Serratia marcesens was examined. The seven new H antigens were demonstrated to be antigenically distinct as determined with Le Minor's H-immobilization test. The H-immobilization antibodies of rabbit anti-H immune sera proved resistant to treatment w...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Traub WH,Fukushima PI

    更新日期:1979-07-01 00:00:00

  • Immunoelectrophoresis for detection of polysaccharides in immune complexes.

    abstract::A procedure for detecting pneumococcal capsular polysaccharides in immune complexes is described. Separation of antigen from immune complexes is achieved by electrophoresis at 56 degrees C. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Coonrod JD,Leach RP

    更新日期:1978-08-01 00:00:00

  • Molecular Diagnosis of Bacterial Vaginosis: an Update.

    abstract::Bacterial vaginosis (BV) is the most common cause of vaginal discharge in reproductive-age women. BV has been associated with poor reproductive outcomes such as preterm delivery, the acquisition of sexually transmitted infections, including HIV, and pelvic inflammatory disease. BV represents the acquisition of a diver...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,评审


    authors: Coleman JS,Gaydos CA

    更新日期:2018-08-27 00:00:00

  • Catheter-related candidemia caused by Candida lipolytica in a patient receiving allogeneic bone marrow transplantation.

    abstract::Candida lipolytica was recovered from the blood and the central venous catheter in a patient receiving allogeneic bone marrow transplantation. Two C. lipolytica strains from different geographical areas and the ATCC 9773 strain of C. lipolytica were used as controls. C. lipolytica was identified by standard methods. M...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: D'Antonio D,Romano F,Pontieri E,Fioritoni G,Caracciolo C,Bianchini S,Olioso P,Staniscia T,Sferra R,Boccia S,Vetuschi A,Federico G,Gaudio E,Carruba G

    更新日期:2002-04-01 00:00:00

  • Detection of synergistic hemolytic activity of Staphylococcus aureus with the Cathra replicator.

    abstract::A replicator method for screening Staphylococcus aureus isolates for synergistic hemolytic activity is described. The technique should also be useful for assessing the hemolytic activity of staphylococci and streptococci on different lots or brands of blood agar because the inoculum and the distances between spot inoc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Boyce JM

    更新日期:1985-05-01 00:00:00