Abstract:
:The human immunodeficiency virus (HIV) was isolated from the blood of 192 of 410 seropositive individuals. Original isolations were made in peripheral blood mononuclear cell (PBMC) cultures, and only one-fifth of the HIV isolates could be adapted to replicate in continuous T-cell lines. Of the 192 HIV isolates, 42 had the characteristic p24 antigen marker of the acquired immunodeficiency syndrome-associated retrovirus type 2 strain of HIV (HIV ARV-2) and 150 resembled the human T-cell lymphotropic virus type III strain of HIV (HIV HTLV-III). Significantly, primary PBMC cultures from two patients yielded multiple variants. When these variants were exposed to continuous T-cell lines, only one of them continued to replicate. The remaining variants were lost and could not be reisolated following passage back into PBMC cultures. We conclude the following from these studies: PBMC cultures are more efficient at isolating HIV than continuous T-cell lines are; some patients harbor more than one genetic variant of HIV in the blood at the same time; and continuous T-cell lines are likely to yield only a portion of the HIV variants originally present in the blood.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Bolton V,Pedersen NC,Higgins J,Jennings M,Carlson Jdoi
10.1128/JCM.25.8.1411-1415.1987subject
Has Abstractpub_date
1987-08-01 00:00:00pages
1411-5issue
8eissn
0095-1137issn
1098-660Xjournal_volume
25pub_type
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.31.7.1896-1898.1993
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doi:10.1128/JCM.30.12.3095-3098.1992
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.36.12.3694-3697.1998
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abstract::After repeated use of fluconazole for therapy of oropharyngeal candidosis, the emergence of in vitro fluconazole-resistant Candida albicans isolates (MIC, > or = 25 micrograms/ml) together with oral candidosis unresponsive to oral dosages of up to 400 mg of fluconazole were observed in patients with human immunodefici...
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doi:10.1128/JCM.32.9.2092-2098.1994
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journal_title:Journal of clinical microbiology
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更新日期:1978-12-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章,评审
doi:10.1128/JCM.32.9.2285-2288.1994
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.17.1.16-21.1983
更新日期:1983-01-01 00:00:00
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更新日期:2003-06-01 00:00:00