Comparison of sample preparation methods for detection of Legionella pneumophila in culture-positive bronchoalveolar lavage fluids by PCR.


:A prospective study was conducted on 25 Legionella pneumophila culture-positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitive with the Chelex-based method (P = 0.03). N difference was found concerning the inhibition rate.


J Clin Microbiol


Jaulhac B,Reyrolle M,Sodahlon YK,Jarraud S,Kubina M,Monteil H,Piémont Y,Etienne J




Has Abstract


1998-07-01 00:00:00












  • Identification of Staphylococcus species of bovine origin with the API Staph-Ident system.

    abstract::The API Staph-Ident system was evaluated as a means for identifying the species of bovine strains of staphylococci routinely isolated from quarter-milk samples. The species identity of 314 of 581 (54%) isolates of staphylococci was correctly determined by this method. The API Staph-Ident system was more accurate in co...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Langlois BE,Harmon RJ,Akers K

    更新日期:1983-11-01 00:00:00

  • Systematic investigation of enrichment media for wild-type Campylobacter jejuni strains.

    abstract::Of the media examined, thioglycolate broth supplemented with 5% lysed sheep blood, Butzler antibiotic mixture, and 0.1% lauryl sulfate was the most sensitive enrichment medium for recovery of wild-type strains of Campylobacter jejuni from cecal contents of chickens and chicken livers. It allowed the retrieval of 1 CFU...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Barot MS,Bokkenheuser VD

    更新日期:1984-07-01 00:00:00

  • Comparison of three commercial rapid identification systems for the unusual gram-positive cocci Dolosigranulum pigrum, Ignavigranum ruoffiae, and Facklamia species.

    abstract::We evaluated three rapid identification systems-The Biomerieux rapid ID 32 STREP (ID32), the BBL Crystal rapid gram-positive identification (Crystal), and the Remel IDS RapID STR (IDS) systems-for their ability to identify 7 strains of Alloiococcus otitidis, 27 strains of Dolosigranulum pigrum, 3 strains of Ignavigran...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: LaClaire LL,Facklam RR

    更新日期:2000-06-01 00:00:00

  • Association of putative enteroaggregative Escherichia coli virulence genes and biofilm production in isolates from travelers to developing countries.

    abstract::Enteroaggregative Escherichia coli (EAEC) is an emerging enteric pathogen that causes acute and chronic diarrhea among children, human immunodeficiency virus-infected patients, and travelers to developing regions of the world. The pathogenesis of EAEC strains involves the production of biofilm. In this study, we deter...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Mohamed JA,Huang DB,Jiang ZD,DuPont HL,Nataro JP,Belkind-Gerson J,Okhuysen PC

    更新日期:2007-01-01 00:00:00

  • Comparison of ribotyping and multilocus enzyme electrophoresis for subtyping of Listeria monocytogenes isolates.

    abstract::Ribotyping was compared with multilocus enzyme electrophoresis (MEE) for subtyping 305 Listeria monocytogenes isolates from clinical and nonclinical sources. For ribotyping, EcoRI-restricted genomic DNA fragments of L. monocytogenes strains were separated by agarose gel electrophoresis, and Southern blots were probed ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Graves LM,Swaminathan B,Reeves MW,Hunter SB,Weaver RE,Plikaytis BD,Schuchat A

    更新日期:1994-12-01 00:00:00

  • Direct Detection of Emergent Fungal Pathogen Candida auris in Clinical Skin Swabs by SYBR Green-Based Quantitative PCR Assay.

    abstract::The recent emergence of the multidrug-resistant and pathogenic yeast Candida auris continues to cause public health concern worldwide. C. auris is alarming because it causes health care-associated outbreaks and can establish invasive infections with high mortality rates. Transmission between patients is facilitated by...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Sexton DJ,Kordalewska M,Bentz ML,Welsh RM,Perlin DS,Litvintseva AP

    更新日期:2018-11-27 00:00:00

  • Clinical relevance of multiple single-nucleotide polymorphisms in Pneumocystis jirovecii Pneumonia: development of a multiplex PCR-single-base-extension methodology.

    abstract::Pneumocystis jirovecii pneumonia (PcP) is a major cause of respiratory illness in patients with AIDS. The identification of multiple single-nucleotide polymorphisms (SNPs) at three distinct P. jirovecii loci encoding dihydrofolate reductase (DHFR), mitochondrial large-subunit rRNA (mtLSU rRNA), and superoxide dismutas...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Esteves F,Gaspar J,De Sousa B,Antunes F,Mansinho K,Matos O

    更新日期:2011-05-01 00:00:00

  • Growth of Vero E-6 cells on microcarriers in a cell bioreactor.

    abstract::Minimum essential medium supplemented with either 7% fetal bovine serum or 2% fetal bovine serum and 1.5% low protein serum replacement (Sigma Chemical Co., St. Louis, Mo.) factor was used to evaluate the growth of Vero 76 cells (E-6 clone) in a cell culture bioreactor. Three different microcarriers, collagen-coated p...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: White LA,Ades EW

    更新日期:1990-02-01 00:00:00

  • Identification of five Peptostreptococcus species isolated predominantly from the female genital tract by using the rapid ID32A system.

    abstract::The rapid ID32A kit (bioMérieux Vitek, Inc., Hazelwood, Mo.) was evaluated for its ability to identify Peptostreptococcus species compared with conventional biochemical tests and gas-liquid chromatography (Virginia Polytechnic Institute), the current "gold standard" method. A total of 5 Peptostreptococcus American Typ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Ng J,Ng LK,Chow AW,Dillon JA

    更新日期:1994-05-01 00:00:00

  • Highly specific and quick detection of Mycobacterium avium subsp. paratuberculosis in feces and gut tissue of cattle and humans by multiple real-time PCR assays.

    abstract::Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease (JD) in cattle and may be associated with Crohn's disease (CD) in humans. It is the slowest growing of the cultivable mycobacteria, and culture from clinical, veterinary, food, or environmental specimens can take 4 months or even lon...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Imirzalioglu C,Dahmen H,Hain T,Billion A,Kuenne C,Chakraborty T,Domann E

    更新日期:2011-05-01 00:00:00

  • Comparison of an enzyme-linked immunosorbent assay with indirect hemagglutination and hemagglutination inhibition for determination of rubella virus antibody: evaluation of immune status with commercial reagents in a clinical laboratory.

    abstract::Comparative evaluations of immune status for rubella virus are described for enzyme-linked immunosorbent assay, hemagglutination inhibition, and indirect hemagglutination. A 92.1% agreement between enzyme-linked immunosorbent assay and indirect hemagglutination assay was demonstrated for rubella immune status. Enzyme-...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Truant AL,Barksdale BL,Huber TW,Elliott LB

    更新日期:1983-01-01 00:00:00

  • Septic arthritis of a native knee joint due to Corynebacterium striatum.

    abstract::We report a case of septic arthritis of a native knee joint due to Corynebacterium striatum, a rare and unusual cause of septic arthritis of native joints. The isolate was identified by a combination of phenotypic, mass spectrometric, and nucleic acid-based assays and exhibited high-level resistance to most antimicrob...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Westblade LF,Shams F,Duong S,Tariq O,Bulbin A,Klirsfeld D,Zhen W,Sakaria S,Ford BA,Burnham CA,Ginocchio CC

    更新日期:2014-05-01 00:00:00

  • Severe progressive subcutaneous abscesses and necrotizing tenosynovitis caused by Rhodococcus aurantiacus.

    abstract::A case of severe progressive subcutaneous abscesses and necrotizing tenosynovitis of the right arm of a 30-year-old woman caused by Rhodococcus aurantiacus is reported. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Tsukamura M,Hikosaka K,Nishimura K,Hara S

    更新日期:1988-02-01 00:00:00

  • Cloning and characterization of a nonhemolytic phospholipase C gene from Burkholderia pseudomallei.

    abstract::We cloned and characterized a phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) gene from Burkholderia pseudomallei. DNA sequence analysis of the gene indicated an open reading frame coding for 700 amino acids with a 34-amino-acid signal peptide. When cleaved, this yields a secreted 73-kDa mature protein. The d...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Korbsrisate S,Suwanasai N,Leelaporn A,Ezaki T,Kawamura Y,Sarasombath S

    更新日期:1999-11-01 00:00:00

  • Amino acid requirements for Legionella pneumophila growth.

    abstract::The amino acids L-arginine, L-isoleucine, L-leucine, L-methionine, L-serine, L-threonine, and L-valine were essential for the growth of Legionella pneumophila in a chemically defined medium. A partial requirement for L-cysteine (or L-cystine) was also observed. A minimal medium containing only the eight required amino...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Tesh MJ,Miller RD

    更新日期:1981-05-01 00:00:00

  • Distinct diversity of vacA, cagA, and cagE genes of Helicobacter pylori associated with peptic ulcer in Japan.

    abstract::Colonization of the stomach mucosa by Helicobacter pylori is a major cause of acute and chronic gastric pathologies in humans. Several H. pylori virulence genes that may play a role in its pathogenicity have been identified. The most important determinants are vacA and cagA in the cag pathogenicity island (cagPAI) gen...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Yamazaki S,Yamakawa A,Okuda T,Ohtani M,Suto H,Ito Y,Yamazaki Y,Keida Y,Higashi H,Hatakeyama M,Azuma T

    更新日期:2005-08-01 00:00:00

  • Evaluation of GeneXpert MTB/RIF for diagnosis of tuberculous meningitis.

    abstract::Tuberculous meningitis (TBM) is the most severe form of tuberculosis. Microbiological confirmation is rare, and treatment is often delayed, increasing mortality and morbidity. The GeneXpert MTB/RIF test was evaluated in a large cohort of patients with suspected tuberculous meningitis. Three hundred seventy-nine patien...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Nhu NT,Heemskerk D,Thu do DA,Chau TT,Mai NT,Nghia HD,Loc PP,Ha DT,Merson L,Thinh TT,Day J,Chau Nv,Wolbers M,Farrar J,Caws M

    更新日期:2014-01-01 00:00:00

  • Serratia rubidaea as an invasive pathogen.

    abstract::Serratia rubidaea biotype 1 was isolated from the bile and blood of a patient with a bile tract carcinoma obstructing the common bile duct and who underwent invasive procedures. The infection was cleared after adequate treatment with antibiotics. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Ursua PR,Unzaga MJ,Melero P,Iturburu I,Ezpeleta C,Cisterna R

    更新日期:1996-01-01 00:00:00

  • Optimal testing conditions for determining MICs and minimum fungicidal concentrations of new and established antifungal agents for uncommon molds: NCCLS collaborative study.

    abstract::This collaborative three-center study evaluated NCCLS M38-A document testing conditions and other testing conditions for the antifungal susceptibility testing of 35 isolates of Aspergillus nidulans, A. terreus, Bipolaris hawaiiensis, B. spicifera, Cladophialophora bantiana, Dactylaria constricta, Fusarium solani, Paec...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Espinel-Ingroff A,Chaturvedi V,Fothergill A,Rinaldi MG

    更新日期:2002-10-01 00:00:00

  • Evaluation and verification of the Seeplex Diarrhea-V ACE assay for simultaneous detection of adenovirus, rotavirus, and norovirus genogroups I and II in clinical stool specimens.

    abstract::Acute viral gastroenteritis is an intestinal infection that can be caused by several different viruses. Here we describe the evaluation and verification of Seeplex Diarrhea-V ACE (Seeplex DV), a novel commercial multiplex reverse transcription-PCR (RT-PCR) assay that detects 5 diarrheal pathogens, including adenovirus...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Higgins RR,Beniprashad M,Cardona M,Masney S,Low DE,Gubbay JB

    更新日期:2011-09-01 00:00:00

  • Measurement of serum D-arabinitol/creatinine ratios for initial diagnosis and for predicting outcome in an unselected, population-based sample of patients with Candida fungemia.

    abstract::D-Arabinitol (DA) is a useful diagnostic marker for candidiasis in patients with neutropenia and other high-risk groups, but its use in unselected patients with a broad range of underlying diseases and conditions has not been studied. We used an automated enzymatic fluorometric assay to measure serum DA/creatinine rat...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Yeo SF,Huie S,Sofair AN,Campbell S,Durante A,Wong B

    更新日期:2006-11-01 00:00:00

  • Adiaspiromycosis causing respiratory failure and a review of human infections due to Emmonsia and Chrysosporium spp.

    abstract::We report a case of a 27-year-old male who presented with respiratory distress that required mechanical ventilation. Transbronchial biopsy revealed adiaspores of the fungus Emmonsia crescens within granulomata, a condition known as adiaspiromycosis. The patient received amphotericin products and corticosteroids, follo...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,评审


    authors: Anstead GM,Sutton DA,Graybill JR

    更新日期:2012-04-01 00:00:00

  • Serotype-specific identification of polioviruses by PCR using primers containing mixed-base or deoxyinosine residues at positions of codon degeneracy.

    abstract::We have developed a method for determining the serotypes of poliovirus isolates by PCR. Three sets of serotype-specific antisense PCR-initiating primers (primers seroPV1A, seroPV2A, and seroPV3A) were designed to pair with codons of VP1 amino acid sequences that are conserved within but that differ across serotypes. T...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kilpatrick DR,Nottay B,Yang CF,Yang SJ,Da Silva E,Peñaranda S,Pallansch M,Kew O

    更新日期:1998-02-01 00:00:00

  • Quantification of bacteria adherent to gastrointestinal mucosa by real-time PCR.

    abstract::The use of real-time quantitative PCR (5' nuclease PCR assay) as a tool to study the gastrointestinal microflora that adheres to the colonic mucosa was evaluated. We developed primers and probes based on the 16S ribosomal DNA gene sequences for the detection of Escherichia coli and Bacteroides vulgatus. DNA was isolat...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Huijsdens XW,Linskens RK,Mak M,Meuwissen SG,Vandenbroucke-Grauls CM,Savelkoul PH

    更新日期:2002-12-01 00:00:00

  • Evaluation of the RapID CB plus system for identification of coryneform bacteria and Listeria spp.

    abstract::In a stress test, the recently introduced RapID CB Plus system (Remel Inc. [formerly Innovative Diagnostic Systems], Norcross, Ga.) was challenged with a diverse set of gram-positive rods comprising 345 strains of coryneform bacteria and 33 strains of Listeria spp. representing a total of 49 different taxa. Overall, w...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Funke G,Peters K,Aravena-Roman M

    更新日期:1998-09-01 00:00:00

  • Diagnostic Accuracy of Stool Xpert MTB/RIF for Detection of Pulmonary Tuberculosis in Children: a Systematic Review and Meta-analysis.

    abstract::Invasive collection methods are often required to obtain samples for the microbiological evaluation of children with presumptive pulmonary tuberculosis (PTB). Nucleic acid amplification testing of easier-to-collect stool samples could be a noninvasive method of diagnosing PTB. We conducted a systematic review and meta...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,meta分析


    authors: MacLean E,Sulis G,Denkinger CM,Johnston JC,Pai M,Ahmad Khan F

    更新日期:2019-05-24 00:00:00

  • Serial quantitative PCR assay for detection, species discrimination, and quantification of Leishmania spp. in human samples.

    abstract::The Leishmania species cause a variety of human disease syndromes. Methods for diagnosis and species differentiation are insensitive and many require invasive sampling. Although quantitative PCR (qPCR) methods are reported for leishmania detection, no systematic method to quantify parasites and determine the species i...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Weirather JL,Jeronimo SM,Gautam S,Sundar S,Kang M,Kurtz MA,Haque R,Schriefer A,Talhari S,Carvalho EM,Donelson JE,Wilson ME

    更新日期:2011-11-01 00:00:00

  • Is Chlamydia pneumoniae present in brain lesions of patients with multiple sclerosis?

    abstract::We investigated the presence of Chlamydia pneumoniae in 81 normal and pathological specimens obtained from postmortem brain tissues of patients with multiple sclerosis and with other neurological or nonneurological diseases. The assays used included PCR amplification of all DNA samples in the initial study. Culture an...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Hammerschlag MR,Ke Z,Lu F,Roblin P,Boman J,Kalman B

    更新日期:2000-11-01 00:00:00

  • Severe Toxoplasma gondii I/III recombinant-genotype encephalitis in a human immunodeficiency virus patient.

    abstract::The reactivation of an uncommon type I/III recombinant-genotype Toxoplasma gondii strain resulted in unusually severe encephalitis and chorioretinitis associated with a cerebral salt wasting syndrome in an African human immunodeficiency virus patient. This observation suggests an influence of the parasite genotype on ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Genot S,Franck J,Forel JM,Rebaudet S,Ajzenberg D,de Paula AM,Dardé ML,Stein A,Ranque S

    更新日期:2007-09-01 00:00:00

  • Comparison of serological reactions of typed Fusobacterium nucleatum strains with those of isolates from humans, canines, and a Macaca mulatta monkey.

    abstract::In the present study, we compared typed F. nucleatum strains with isolates from various human periodontal diseases, canines, and a Macaca mulatta monkey. All isolates displayed biochemical reactions similar to those of the typed strains in the API 20A system (Analytab Products, Plainville, N.Y.). The human and monkey ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Vincent JW,Falkler WA Jr,Craig JA

    更新日期:1983-04-01 00:00:00