Comparison of sample preparation methods for detection of Legionella pneumophila in culture-positive bronchoalveolar lavage fluids by PCR.

abstract：:Fosfomycin has been shown to have a wide spectrum of activity against multidrug-resistant Gram-negative bacteria; however, breakpoints have been established only for Escherichia coli or Enterobacterales per the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibilit...

journal_title：Journal of clinical microbiology

authors： Smith EC,Brigman HV,Anderson JC,Emery CL,Bias TE,Bergen PJ,Landersdorfer CB,Hirsch EB

更新日期：2020-09-22 00:00:00

abstract：:The diagnosis of Clostridium difficile infection continues to be a challenge for many clinical microbiology laboratories. A new lateral flow assay, the C.Diff Quik Chek Complete assay, which tests for the presence of both glutamate dehydrogenase (GDH) and C. difficile toxins A and B, was evaluated for its ability to d...

journal_title：Journal of clinical microbiology

authors： Sharp SE,Ruden LO,Pohl JC,Hatcher PA,Jayne LM,Ivie WM

更新日期：2010-06-01 00:00:00

abstract：:Rose Bengal and counterimmunoelectrophoresis, two tests that detect antibodies against different structural antigens, when carried out on spinal fluid permitted rapid diagnosis of human Brucella meningitis. The Rose Bengal test was positive in five out of five patients studied, and counterimmunoelectrophoresis was pos...

journal_title：Journal of clinical microbiology

authors： Díaz R,Maraví-Poma E,Delgado G,Rivero A

更新日期：1978-02-01 00:00:00

abstract：:To evaluate the risk of a nosocomial spread of Pneumocystis carinii f. sp. hominis (P. carinii hominis), air filter samples from rooms of P. carinii pneumonia (PCP) patients, adjacent corridors, and other hospital environments have been investigated for the presence of P. carinii hominis. Amplified DNA from air filter...

journal_title：Journal of clinical microbiology

authors： Olsson M,Lidman C,Latouche S,Björkman A,Roux P,Linder E,Wahlgren M

更新日期：1998-06-01 00:00:00

abstract：:During a 3-year period, all Pasteurella strains recovered at the Clinical Microbiological Laboratory, Lund, Sweden, were studied biochemically with respect to their relationship to the recently described taxa of this genus. Of 159 strains recovered from 146 infected humans, 95 were identified as Pasteurella multocida ...

journal_title：Journal of clinical microbiology

authors： Holst E,Rollof J,Larsson L,Nielsen JP

更新日期：1992-11-01 00:00:00

abstract：:Comparative evaluations of immune status for rubella virus are described for enzyme-linked immunosorbent assay, hemagglutination inhibition, and indirect hemagglutination. A 92.1% agreement between enzyme-linked immunosorbent assay and indirect hemagglutination assay was demonstrated for rubella immune status. Enzyme-...

journal_title：Journal of clinical microbiology

authors： Truant AL,Barksdale BL,Huber TW,Elliott LB

更新日期：1983-01-01 00:00:00

abstract：:We demonstrate that all nine species of the Burkholderia cepacia complex can express the mucoid phenotype. A survey of clinical isolates showed that strains of B. cenocepacia, the most virulent species of the complex, are most frequently nonmucoid. Additionally, isolates from patients with chronic infections can conve...

journal_title：Journal of clinical microbiology

authors： Zlosnik JE,Hird TJ,Fraenkel MC,Moreira LM,Henry DA,Speert DP

更新日期：2008-04-01 00:00:00

abstract：:Accurate identification of mycobacterial species and subspecies is essential to evaluate their significance and to perform epidemiological studies. The subspecies of Mycobacterium avium have different attributes but coincide in their zoonotic potential. Our knowledge about M. avium subsp. silvaticum is limited, since ...

journal_title：Journal of clinical microbiology

authors： Rónai Z,Csivincsik Á,Dán Á

更新日期：2015-05-01 00:00:00

abstract：:Human papillomavirus (HPV) is causally associated with anal cancer, as HPV DNA is detected in up to 90% of anal intraepithelial neoplasias and anal cancers. With the gradual increase of anal cancer rates, there is a growing need to establish reliable and clinically relevant methods to detect anal cancer precursors. In...

journal_title：Journal of clinical microbiology

authors： Low HC,Silver MI,Brown BJ,Leng CY,Blas MM,Gravitt PE,Woo YL

更新日期：2015-02-01 00:00:00

abstract：:We evaluated the Prodesse ProFlu-1 real-time reverse transcription-PCR multiplex assay with the SmartCycler instrument for the detection of human respiratory syncytial virus (RSV) and influenza A and B viruses in comparison to conventional cell culture and antigen immunoassays with the BD Directigen A+B and Binax NOW ...

journal_title：Journal of clinical microbiology

authors： Liao RS,Tomalty LL,Majury A,Zoutman DE

更新日期：2009-03-01 00:00:00

abstract：:Patients with chronic granulomatous disease have a marked defect in neutrophil oxidative metabolism and microbicidal activity. Asymptomatic mothers of males with the disease can usually be identified as heterozygous carriers by intermediate leukocyte function. Most mothers of females with the disease, however, have no...

journal_title：Journal of clinical microbiology

authors： Mills EL,Rholl KS,Quie PG

更新日期：1980-07-01 00:00:00

abstract：:Nocardia cyriacigeorgica is a recently characterized species within the genus of Nocardia. We report a brain abscess, following a primary pulmonary colonization, due to this species in a human immunodeficiency virus-infected patient. This case confirms that isolation of Nocardia in sputum is associated with a high ris...

journal_title：Journal of clinical microbiology

authors： Barnaud G,Deschamps C,Manceron V,Mortier E,Laurent F,Bert F,Boiron P,Vinceneux P,Branger C

更新日期：2005-09-01 00:00:00

abstract：:The diagnosis of syphilis is challenging and often relies on serologic tests to detect treponemal or nontreponemal antibodies. Recently, the Centers for Disease Control and Prevention and the Association of Public Health Laboratories proposed an update to the syphilis serology testing algorithm, in which serum samples...

journal_title：Journal of clinical microbiology

authors： Binnicker MJ,Jespersen DJ,Rollins LO

更新日期：2011-04-01 00:00:00

abstract：:When Shiga toxin-producing Escherichia coli (STEC) strains emerged as agents of human disease, two types of toxin were identified: Shiga toxin type 1 (Stx1) (almost identical to Shiga toxin produced by Shigella dysenteriae type 1) and the immunologically distinct type 2 (Stx2). Subsequently, numerous STEC strains have...

journal_title：Journal of clinical microbiology

authors： Scheutz F,Teel LD,Beutin L,Piérard D,Buvens G,Karch H,Mellmann A,Caprioli A,Tozzoli R,Morabito S,Strockbine NA,Melton-Celsa AR,Sanchez M,Persson S,O'Brien AD

更新日期：2012-09-01 00:00:00

abstract：:A multiplex PCR (mPCR) protocol was developed for simultaneous detection of the gyrB gene in Streptococcus pneumoniae, Streptococcus mitis, and Streptococcus oralis, and the specificity was evaluated using 141 coccus strains. Genomic DNAs purified from S. pneumoniae, S. mitis, and S. oralis strains were efficiently de...

journal_title：Journal of clinical microbiology

authors： Kim W,Park HK,Hwang WJ,Shin HS

更新日期：2013-03-01 00:00:00

abstract：:The CLSI (formerly NCCLS) M38-A document for antifungal susceptibility testing of filamentous fungi does not describe guidelines for echinocandins. A multicenter study (eight centers) evaluated inter- and intralaboratory reproducibilities of two reading times (24 and 48 h or 48 and 72 h) and two end points (MICs and m...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A,Fothergill A,Ghannoum M,Manavathu E,Ostrosky-Zeichner L,Pfaller MA,Rinaldi MG,Schell W,Walsh TJ

更新日期：2007-07-01 00:00:00

abstract：:Two hundred seventy nasopharyngeal aspirates were tested in duplicate with the Ortho Diagnostics, Inc. (Raritan, N.J.), respiratory syncytial virus antigen enzyme-linked immunosorbent assay. The test was sensitive (80 to 82%) and specific (96%) when compared with cell culture. The enzyme-linked immunosorbent assay det...

journal_title：Journal of clinical microbiology

authors： Bromberg K,Tannis G,Daidone B,Clarke L,Sierra MF

更新日期：1985-12-01 00:00:00

abstract：:Comparative sequence analysis was performed upon Bacillus anthracis and its closest relatives, B. cereus and B. thuringiensis. Portions of rpoB DNA from 10 strains of B. anthracis, 16 of B. cereus, 10 of B. thuringiensis, 1 of B. mycoides, and 1 of B. megaterium were amplified and sequenced. The determined rpoB sequen...

journal_title：Journal of clinical microbiology

authors： Ko KS,Kim JM,Kim JW,Jung BY,Kim W,Kim IJ,Kook YH

更新日期：2003-07-01 00:00:00

abstract：:The indirect immunoperoxidase antibody technique (IPA) has been applied to determine immunoglobulin (Ig)G to humans cytomegalovirus (CMV) antibodies in 114 blood donor sera, four cases of congenital cytomegalic inclusion disease, and four cases of acquired CMV infection. The results have been compared with those obtai...

journal_title：Journal of clinical microbiology

authors： Gerna G,McCloud CJ,Chambers RW

更新日期：1976-03-01 00:00:00

abstract：:The timely and exact diagnosis of prosthetic joint infection (PJI) is crucial for surgical decision-making. Intraoperatively, delivery of the result within an hour is required. Alpha-defensin lateral immunoassay of joint fluid (JF) is precise for the intraoperative exclusion of PJI; however, for patients with a limite...

journal_title：Journal of clinical microbiology

authors： Fillerova R,Gallo J,Radvansky M,Kraiczova V,Kudelka M,Kriegova E

更新日期：2017-09-01 00:00:00

abstract：:Campylobacter species are the leading agents of bacterial gastroenteritis in developed countries. In this study 320 specimens of feces from patients with symptoms of acute gastroenteritis were cultured for Campylobacter species by direct plating on modified charcoal cefoperazone deoxycholate agar and by enrichment in ...

journal_title：Journal of clinical microbiology

authors： Maher M,Finnegan C,Collins E,Ward B,Carroll C,Cormican M

更新日期：2003-07-01 00:00:00

abstract：:This study assesses human papillomavirus (HPV) detection and genotyping in self-sampled genital smears applied to an indicating FTA elute cartridge (FTA cartridge). The study group consisted of 96 women, divided into two sample sets. All samples were analyzed by the HPV SPF(10)-Line Blot 25. Set 1 consisted of 45 wome...

journal_title：Journal of clinical microbiology

authors： Lenselink CH,de Bie RP,van Hamont D,Bakkers JM,Quint WG,Massuger LF,Bekkers RL,Melchers WJ

更新日期：2009-08-01 00:00:00

abstract：:A simple solid-phase enzyme-linked immunosorbent assay procedure for the detection of human antibodies to Entamoeba histolytica was developed which showed a high degree of correlation with the agar gel diffusion, counterelectrophoresis, and indirect hemagglutination methods, as well as with clinical data. The enzyme-l...

journal_title：Journal of clinical microbiology

authors： Lin TM,Halbert SP,Chiu CT,Zarco R

更新日期：1981-04-01 00:00:00

abstract：:The use of rapid-cycle PCR combined with colorimetric microwell hybridization for detecting Bordetella pertussis was investigated. Rapid cycling was performed with an air thermocycler (model 1605; Idaho Technology, Idaho Falls, Idaho). Although the instrument was originally designed to be used with capillary tubes, an...

journal_title：Journal of clinical microbiology

authors： Buck GE

更新日期：1996-06-01 00:00:00

abstract：:An ATP-based biocide susceptibility assay for mycobacteria was developed by optimizing the cell lysis and assay conditions. Compared to the conventional agar plating method, the assay was rapid (1.5 h) and showed high sensitivity and specificity as determined by receiver operating characteristic (ROC) analysis. The te...

journal_title：Journal of clinical microbiology

authors： Kapoor R,Yadav JS

更新日期：2010-10-01 00:00:00

abstract：:Febrile travelers from countries with unique endemic pathogens pose a significant diagnostic challenge. In this report, we describe the case of a Tongan man presenting with fever, rash, and altered mental status. The diagnosis of Chikungunya encephalitis was made using a laboratory-developed real-time RT-PCR and serol...

journal_title：Journal of clinical microbiology

authors： Nelson J,Waggoner JJ,Sahoo MK,Grant PM,Pinsky BA

更新日期：2014-09-01 00:00:00

abstract：:Chronic periodontitis is a common infectious disease in the adult population. The etiology is clearly bacterial, and a small number of bacterial species have been consistently associated with periodontitis, including Bacteroides forsythus and Porphyromonas gingivalis. Comparatively little attention has been paid to th...

journal_title：Journal of clinical microbiology

authors： Leys EJ,Lyons SR,Moeschberger ML,Rumpf RW,Griffen AL

更新日期：2002-03-01 00:00:00

abstract：:The iC-GPC Assay (iCubate, Huntsville, AL) is a qualitative multiplex test for the detection of five of the most common Gram-positive bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Enterococcus faecalis, and Enterococcus faecium) responsible for bacterial bloodstream infections,...

journal_title：Journal of clinical microbiology

authors： Granato PA,Unz MM,Widen RH,Silbert S,Young S,Heflin KL,Conover MS,Buchan BW,Ledeboer NA

更新日期：2018-08-27 00:00:00

abstract：:The BD Phoenix system was evaluated for species-level identification of yeasts (250 clinical isolates) and compared with the Vitek 2 system, using ribosomal internal transcribed spacer (ITS) sequence analysis as the gold standard. Considering only the species included in each system's database, 96.3% (236/245) and 91....

journal_title：Journal of clinical microbiology

authors： Posteraro B,Ruggeri A,De Carolis E,Torelli R,Vella A,De Maio F,Ricciardi W,Posteraro P,Sanguinetti M

更新日期：2013-11-01 00:00:00

abstract：:RNA of the newly identified human metapneumovirus (HMPV) was detected in nasopharyngeal aspirates of 11 of 63 (17.5%) young children with respiratory tract disease. Markers of infection caused by another member of the Pneumovirinae subfamily of the family Paramyxoviridae, respiratory syncytial virus (RSV), were identi...

journal_title：Journal of clinical microbiology

authors： Viazov S,Ratjen F,Scheidhauer R,Fiedler M,Roggendorf M

更新日期：2003-07-01 00:00:00