Comparison of sample preparation methods for detection of Legionella pneumophila in culture-positive bronchoalveolar lavage fluids by PCR.

Abstract:

:A prospective study was conducted on 25 Legionella pneumophila culture-positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitive with the Chelex-based method (P = 0.03). N difference was found concerning the inhibition rate.

journal_name

J Clin Microbiol

authors

Jaulhac B,Reyrolle M,Sodahlon YK,Jarraud S,Kubina M,Monteil H,Piémont Y,Etienne J

doi

10.1128/JCM.36.7.2120-2122.1998

subject

Has Abstract

pub_date

1998-07-01 00:00:00

pages

2120-2

issue

7

eissn

0095-1137

issn

1098-660X

journal_volume

36

pub_type

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