Clinical Evaluation of the iCubate iC-GPC Assay for Detection of Gram-Positive Bacteria and Resistance Markers from Positive Blood Cultures.

abstract：:Human herpesvirus 6 (HHV-6) variant B is frequently identified in peripheral blood, but identification of HHV-6 variant A is relatively rare. We devised a PCR-based method for sensitive, simultaneous detection of both HHV-6 variants. The method was applied to 34 lung tissue specimens that were previously shown to cont...

journal_title：Journal of clinical microbiology

authors： Cone RW,Huang ML,Hackman RC,Corey L

更新日期：1996-04-01 00:00:00

abstract：:Analysis of hospital-acquired methicillin-resistant Staphylococcus aureus strains isolated from a tertiary public hospital in Singapore revealed that multisusceptible strains had gradually started to replace the endemic multiresistant strain (ST239-MRSA-III) since 2002. Molecular typing showed that this was a predomin...

journal_title：Journal of clinical microbiology

authors： Hsu LY,Koh TH,Singh K,Kang ML,Kurup A,Tan BH

更新日期：2005-06-01 00:00:00

abstract：:Vibrio cholerae serotype O1 organisms that do not produce cholera toxin and, in fact, lack the genetic material encoding the enterotoxin have recently been detected in coastal regions of the United States. Although these organisms have been assumed to be nonpathogenic, they have been considered a potential reservoir o...

journal_title：Journal of clinical microbiology

authors： Johnston JM,McFarland LM,Bradford HB,Caraway CT

更新日期：1983-05-01 00:00:00

abstract：:A new disposable plastic tube device and technique for ovum and parasite concentration, with upper and lower chambers separated by a fine screen, was compared with the standard gauze and open-funnel filtration technique as well as with the technique of direct smearing with an acid-fast stain. The new concentrator was ...

journal_title：Journal of clinical microbiology

authors： Zierdt WS

更新日期：1984-11-01 00:00:00

abstract：:Amplified fragment length polymorphism (AFLP) was employed as a genetic analysis tool for the study of the genetic relatedness of Mycobacterium avium subsp. paratuberculosis isolates harvested from bovine fecal samples and from bovine or human tissues. This analysis revealed genetic differences between these two isola...

journal_title：Journal of clinical microbiology

authors： O'Shea B,Khare S,Klein P,Roussel A,Adams LG,Ficht TA,Rice-Ficht AC

更新日期：2011-06-01 00:00:00

abstract：:A 25-year-old female was admitted to our intensive care unit with septic shock and multiorgan failure caused by extended-spectrum beta-lactamase-producing Escherichia coli originating from the right renal pelvis. A 16-day course of treatment with meropenem reversed the septic condition, but the infection recurred ther...

journal_title：Journal of clinical microbiology

authors： Krueger WA,Kempf VA,Peiffer M,Nagele U,Unertl KE,Schroeder TH

更新日期：2008-02-01 00:00:00

abstract：:We compared the new Abbott TestPack (TP) respiratory syncytial virus (RSV) enzyme immunoassay (EIA) with cell culture and two commercial RSV EIAs (from Abbott Diagnostics and Kallestad Laboratories) by using split samples of fresh nasal washings from children with suspected RSV disease. Two tubes of HEp-2 cells were i...

journal_title：Journal of clinical microbiology

authors： Wren CG,Bate BJ,Masters HB,Lauer BA

更新日期：1990-06-01 00:00:00

abstract：:We analyzed a collection of 97 well-characterized Burkholderia cepacia genomovar III isolates to evaluate multiple genomic typing systems, including pulsed-field gel electrophoresis (PFGE), BOX-PCR fingerprinting and random amplified polymorphic DNA (RAPD) typing. The typeability, reproducibility, and discriminatory p...

journal_title：Journal of clinical microbiology

authors： Coenye T,Spilker T,Martin A,LiPuma JJ

更新日期：2002-09-01 00:00:00

abstract：:A new, rapid method for the detection of human immunodeficiency virus type 1 (HIV-1) antibody by time-resolved fluoroimmunoassay (TR-FIA) was developed. In this assay format, microtitration strips were coated with a recombinant fusion protein, and the same protein was labeled with europium and added into the wells sim...

journal_title：Journal of clinical microbiology

authors： Siitari H,Turunen P,Schrimsher J,Nunn M

更新日期：1990-09-01 00:00:00

abstract：:Analysis of the electrophoretic profiles of the outer membrane proteins could be used to differentiate Campylobacter jejuni (16 strains) from Campylobacter coli (10 strains). This observation was confirmed by the study of DNA homology obtained by a quantitative filter hybridization method. The hippurate hydrolysis tes...

journal_title：Journal of clinical microbiology

authors： Derclaye I,Delor I,Van Bouchaute M,Moureau P,Wauters G,Cornelis GR

更新日期：1989-05-01 00:00:00

abstract：:From November 2006 to April 2007, nine nonrepetitive isolates of Klebsiella pneumoniae with reduced susceptibility or resistance to carbapenems were recovered from clinical specimens from separate patients hospitalized in a tertiary care hospital. The imipenem-EDTA synergy test was positive for all isolates. PCR, sequ...

journal_title：Journal of clinical microbiology

authors： Tokatlidou D,Tsivitanidou M,Pournaras S,Ikonomidis A,Tsakris A,Sofianou D

更新日期：2008-03-01 00:00:00

abstract：:During lyssavirus surveillance, 1,221 bats of at least 30 species were collected from 25 locations in Kenya. One isolate of Lagos bat virus (LBV) was obtained from a dead Eidolon helvum fruit bat. The virus was most similar phylogenetically to LBV isolates from Senegal (1985) and from France (imported from Togo or Egy...

journal_title：Journal of clinical microbiology

authors： Kuzmin IV,Niezgoda M,Franka R,Agwanda B,Markotter W,Beagley JC,Urazova OY,Breiman RF,Rupprecht CE

更新日期：2008-04-01 00:00:00

abstract：:Molecular techniques such as spa typing and multilocus sequence typing use DNA sequence data for differentiating Staphylococcus aureus isolates. Although spa typing is capable of detecting both genetic micro- and macrovariation, it has less discriminatory power than the more labor-intensive pulsed-field gel electropho...

journal_title：Journal of clinical microbiology

authors： Koreen L,Ramaswamy SV,Naidich S,Koreen IV,Graff GR,Graviss EA,Kreiswirth BN

更新日期：2005-08-01 00:00:00

abstract：:The herpes simplex virus type 1 (HSV-1) genome is a linear double-stranded DNA of 152 kpb. It is divided into long and short regions of unique sequences termed U(L) and U(S), respectively, and these are flanked by regions of inverted internal and terminal repeats. Microsatellites are short tandem repeats of 1- to 6-nu...

journal_title：Journal of clinical microbiology

authors： Deback C,Boutolleau D,Depienne C,Luyt CE,Bonnafous P,Gautheret-Dejean A,Garrigue I,Agut H

更新日期：2009-03-01 00:00:00

abstract：:Sera from goats suffering from caprine arthritis-encephalitis contained antibodies to virus proteins of 15, 17, 28, 40, and 130 kilodaltons in immunoblots of maedi-visna virus. We propose to use immunoblotting as a validation test for enzyme-linked immunosorbent assay and demonstrate that the specificity of indirect e...

journal_title：Journal of clinical microbiology

authors： Zanoni R,Krieg A,Peterhans E

更新日期：1989-03-01 00:00:00

abstract：:A modified auxanographic carbohydrate assimilation procedure for the identification of medically important yeasts is described. This method employs a heavy inoculum of unstarved yeasts, autoclaved yeast assimilation medium, pour plates of shallow depth, and commercially available carbohydrate-impregnated disks. The ac...

journal_title：Journal of clinical microbiology

authors： Mickelsen PA,McCarthy LR,Propst MA

更新日期：1977-03-01 00:00:00

abstract：:Two serotypes of exfoliatin, A and B, previously reported by Kondo et al. (1974) were examined for their presence in 43 strains of Staphylococcus aureus, most of which were isolated from patients with Ritter's disease and impetigo. The tested strains consisted of 24 strains of phage group II and 19 strains not of phag...

journal_title：Journal of clinical microbiology

authors： Kondo I,Sakurai S,Sarai Y,Futaki S

更新日期：1975-05-01 00:00:00

abstract：:The first outbreak caused by vancomycin-resistant enterococci of the VanB phenotype in Poland was analyzed. It occurred in a single ward of a Warsaw hospital which is a specialized center for the treatment of hematological disorders. Between July 1999 and February 2000, 11 patients in the ward were found to be infecte...

journal_title：Journal of clinical microbiology

authors： Kawalec M,Gniadkowski M,Zaleska M,Ozorowski T,Konopka L,Hryniewicz W

更新日期：2001-05-01 00:00:00

abstract：:Between November 1983 and January 1984, 13 cases of Candida parapsilosis endophthalmitis occurred in Florida, Georgia, and Tennessee in patients who had had an intraocular lens implantation (IOLI) or cataract extraction with an IOLI. This outbreak followed the introduction in July 1983 of a new brand of balanced salt ...

journal_title：Journal of clinical microbiology

authors： McCray E,Rampell N,Solomon SL,Bond WW,Martone WJ,O'Day D

更新日期：1986-10-01 00:00:00

abstract：:Human papillomavirus (HPV) DNA detection and typing are important for diagnosis and management of HPV-associated diseases. One of the most commonly used PCR methods, GP5+/6+, shows weaknesses in amplifying certain types. To circumvent this limitation, we developed and validated broad-spectrum primers targeting the GP5...

journal_title：Journal of clinical microbiology

authors： Schmitt M,Dondog B,Waterboer T,Pawlita M

更新日期：2008-03-01 00:00:00

abstract：:Murine monoclonal antibodies (MAbs) against herpes simplex virus type 1 and 2 (HSV-1 and -2, respectively) nuclear antigens were used to identify cells infected with HSV-1 or -2 by indirect immunofluorescence in conjunction with flow cytometry after virus amplification of MRC-5 cell monolayers. The results indicate th...

journal_title：Journal of clinical microbiology

authors： McSharry JJ,Costantino R,McSharry MB,Venezia RA,Lehman JM

更新日期：1990-08-01 00:00:00

abstract：:Eleven isolates representing five distinct outbreaks of pneumococcal conjunctivitis were examined for phenotypic and genetic characteristics. None of the strains possessed capsules, and all strains were susceptible to optochin, bile soluble, and Gen-Probe AccuProbe test positive. All 11 isolates were confirmed as Stre...

journal_title：Journal of clinical microbiology

authors： Carvalho MG,Steigerwalt AG,Thompson T,Jackson D,Facklam RR

更新日期：2003-09-01 00:00:00

abstract：:The Y448H mutation in NS5B has been selected by GS-9190 as well as several benzothiadiazine hepatitis C virus (HCV) polymerase inhibitors in vitro and in vivo. However, the level and the evolution kinetics of this resistance mutation prior to and during treatment are poorly understood. In this study, we developed an a...

journal_title：Journal of clinical microbiology

authors： Bae AS,Ku KS,Miller MD,Mo H,Svarovskaia ES

更新日期：2011-09-01 00:00:00

abstract：:Tropheryma whipplei, the agent of Whipple's disease, is a gram-positive rod-shaped bacterium that belongs to the group of actinobacteria. In order to produce monoclonal antibodies (MAbs) against this bacterium, we inoculated mice with two different strains, Slow2 and Endo5. We produced 13 and 10 MAbs against Slow2 and...

journal_title：Journal of clinical microbiology

authors： Yu Y,Kowalczewska M,Decloquement P,Nappez C,La Scola B

更新日期：2006-11-01 00:00:00

abstract：:Bartonella henselae uveitis was diagnosed in a 40-year-old woman with underlying HLA-B27 uveitis on the basis of immunodetection and molecular detection of the organism, a Marseilles genogroup and a CAL-1 genotype strain, in the vitreous fluid. This case illustrates that B. henselae should be included in the different...

journal_title：Journal of clinical microbiology

authors： Drancourt M,Bodaghi B,Lepidi H,Le Hoang P,Raoult D

更新日期：2004-04-01 00:00:00

abstract：:Repetitive sequence-based PCR (rep-PCR) and amplified fragment length polymorphism (AFLP) were used to characterize a sample of 43 field isolates and 4 attenuated vaccine strains of Pasteurella multocida recovered from multiple avian species. Both rep-PCR and AFLP assays were rapid and reproducible, with high indices ...

journal_title：Journal of clinical microbiology

authors： Amonsin A,Wellehan JF,Li LL,Laber J,Kapur V

更新日期：2002-08-01 00:00:00

abstract：:Unbiased nontargeted metagenomic RNA sequencing (UMERS) has the advantage to detect known as well as unknown pathogens and, thus, can significantly improve the detection of viral, bacterial, parasitic, and fungal sequences in public health settings. In particular, conventional diagnostic methods successfully identify ...

journal_title：Journal of clinical microbiology

authors： Fischer N,Indenbirken D,Meyer T,Lütgehetmann M,Lellek H,Spohn M,Aepfelbacher M,Alawi M,Grundhoff A

更新日期：2015-07-01 00:00:00

abstract：:A TaqMan PCR was developed for quantifying early colonizer microorganisms in dental biofilms. To design species-specific primers and TaqMan probes, genomic subtractive hybridization was used. This quantitative assay in combination with subtractive hybridization may be of value in the study of microbial ecosystems cons...

journal_title：Journal of clinical microbiology

authors： Suzuki N,Nakano Y,Yoshida A,Yamashita Y,Kiyoura Y

更新日期：2004-08-01 00:00:00

abstract：:The comparative susceptibilities for rhinovirus isolation were determined for strain MRC-5 human embryonic lung fibroblast and human fetal tonsil cells. Samples from nasopharyngeal and throat swab clinical specimens were inoculated onto duplicate monolayers of each cell type. Of 105 rhinovirus-positive specimens, 78 (...

journal_title：Journal of clinical microbiology

authors： Geist FC,Hayden FG

更新日期：1985-09-01 00:00:00

abstract：:A method of inactivating mycoplasma contaminants in sera by using binary ethyleneimine was tried. When used at the concentration recommended for inactivating viruses, 0.001 M, binary ethyleneimine inactivated only two of five mycoplasma species. Even at a concentration of 0.01 M, the compound did not consistently inac...

journal_title：Journal of clinical microbiology

authors： Christianson GG,Pemberton JR,Koski TA

更新日期：1980-04-01 00:00:00