Sequence variation in Shigella sonnei (Sonnei), a pathogenic clone of Escherichia coli, over four continents and 41 years.

abstract：:A total of 800 colonies suggestive of Salmonella, Shigella, or Yersinia species isolated on stool differential agar media were inoculated onto both conventional biochemical test media (triple sugar iron agar, urea agar, and phenylalanine agar) and Entero Pathogen Screen cards of the AutoMicrobic system (Vitek Systems,...

journal_title：Journal of clinical microbiology

authors： Villasante PA,Agulla A,Merino FJ,Pérez T,Ladrón de Guevara C,Velasco AC

更新日期：1987-03-01 00:00:00

abstract：:Two new T-cell-based reporter cell lines were established to measure human immunodeficiency virus type 1 (HIV-1) infectivity. One cell line naturally expresses CD4 and CXCR4, making it susceptible to X4-tropic viruses, and the other cell line, in which a CCR5 expression vector was introduced, is susceptible to both X4...

journal_title：Journal of clinical microbiology

authors： Chiba-Mizutani T,Miura H,Matsuda M,Matsuda Z,Yokomaku Y,Miyauchi K,Nishizawa M,Yamamoto N,Sugiura W

更新日期：2007-02-01 00:00:00

abstract：:A new DNA probe assay (INNO LiPA Mycobacteria; Innogenetics, Ghent, Belgium) for the simultaneous identification, by means of reverse hybridization and line-probe technology, of Mycobacterium tuberculosis complex, Mycobacterium kansasii, Mycobacterium xenopi, Mycobacterium gordonae, the species of the Mycobacterium av...

journal_title：Journal of clinical microbiology

authors： Tortoli E,Nanetti A,Piersimoni C,Cichero P,Farina C,Mucignat G,Scarparo C,Bartolini L,Valentini R,Nista D,Gesu G,Tosi CP,Crovatto M,Brusarosco G

更新日期：2001-03-01 00:00:00

abstract：:This study investigated a rare area of endemicity with a high prevalence of both hepatitis B virus (HBV) and hepatitis C virus (HCV) infections, in Putian County, China. Among 1,050 subjects, the overall prevalence of HBsAg seropositivity was 15.8%, and that of anti-HCV seropositivity was 28.9%. Intrafamilial viral tr...

journal_title：Journal of clinical microbiology

authors： Lu WP,Lin GX,Shi S,Dong JH

更新日期：2012-06-01 00:00:00

abstract：:Of 100 strains of enterococci, 83% reduced litmus milk within 4 h. None of the 100 non-enterococcal streptococci tested were positive. ...

journal_title：Journal of clinical microbiology

authors： Schierl EA,Blazevic DJ

更新日期：1981-08-01 00:00:00

abstract：:The incidence and RNA electropherotypes of rotavirus in stools or rectal swabs of children with diarrhea were studied for three rotavirus seasons (1981 through 1984) in Philadelphia, Pa. We used a simplified RNA analysis method involving polyacrylamide gel electrophoresis followed by silver staining. Phosphate-buffere...

journal_title：Journal of clinical microbiology

authors： Dolan KT,Twist EM,Horton-Slight P,Forrer C,Bell LM Jr,Plotkin SA,Clark HF

更新日期：1985-05-01 00:00:00

abstract：:Twenty-one reference strains and 88 clinical isolates of Aerococcus, Enterococcus, and Streptococcus species were tested for reactions in the Rapid Strep (RS) and modified conventional tests. We conclude that some but not all of the tests in the RS system could be used to substitute for conventional tests. RS tests fo...

journal_title：Journal of clinical microbiology

authors： Fertally SS,Facklam R

更新日期：1987-10-01 00:00:00

abstract：:Group B Streptococcus detection directly from Copan ESwab collected samples, using the BD Max GBS assay, was evaluated on receipt in the laboratory and after 24 h at room temperature. Results were compared to those using Lim broth enrichment PCR and culture. No significant difference was observed between 24 h ESwab an...

journal_title：Journal of clinical microbiology

authors： Silbert S,Rocchetti TT,Gostnell A,Kubasek C,Widen R

更新日期：2016-06-01 00:00:00

abstract：:Staphylococcus aureus produced an inhibitory factor that suppressed slime formation by Psuudomonas aeruginosa but had little effect on the growth of the organism. The inhibitory factor was not found in broth cultures but could be extracted from cultures grown on solid agar. The inhibitory factor moderately inhibited g...

journal_title：Journal of clinical microbiology

authors： Shibl AM,Al-Sowaygh IA

更新日期：1980-02-01 00:00:00

abstract：:We developed a 4-h nested polymerase chain reaction assay that detected a region of the plasminogen activator gene of Yersinia pestis in 100% of 43 Y. pestis strains isolated from humans, rats, and fleas yet was unreactive with the closely related species Yersinia enterocolitica and Yersinia pseudotuberculosis. ...

journal_title：Journal of clinical microbiology

authors： Campbell J,Lowe J,Walz S,Ezzell J

更新日期：1993-03-01 00:00:00

abstract：:Shiga toxin (Stx)-producing Escherichia coli (STEC) is the main cause of postdiarrheal hemolytic-uremic syndrome (HUS), a life-threatening clinical complication characterized by hemolytic anemia, thrombocytopenia, and acute renal failure that mainly affects children. A relevant feature of STEC strains is the productio...

journal_title：Journal of clinical microbiology

authors： Melli LJ,Zylberman V,Hiriart Y,Lauche CE,Baschkier A,Pardo R,Miliwebsky E,Chinen I,Rivas M,Goldbaum FA,Ugalde JE,Comerci DJ,Ciocchini AE

更新日期：2020-02-24 00:00:00

abstract：:Serial cerebrospinal fluid samples were obtained from 14 multiple sclerosis patients over a period of several months and were tested under code for Inoue-Melnick virus. In five of the positive patients, virus was present in 28 of 32 specimens collected over a period of 2 to 5 months. Four patients, from whom a total o...

journal_title：Journal of clinical microbiology

authors： Melnick JL,Seidel E,Wang SS,Muchinik G,Rashti EJ,Jacobs LD,Freeman AI,O'Malley J

更新日期：1985-10-01 00:00:00

abstract：:Twenty-nine Borrelia burgdorferi isolates, obtained from dusky-footed wood rats (Neotoma fuscipes) and California kangaroo rats (Dipodomys californicus) in California, were analyzed genetically. Chromosomal DNA was examined by restriction endonuclease analysis (REA) and gene probe restriction fragment length polymorph...

journal_title：Journal of clinical microbiology

authors： Zingg BC,Brown RN,Lane RS,LeFebvre RB

更新日期：1993-12-01 00:00:00

abstract：:Ten characteristic strains of spirochetes (HT2, HT7, HT10, HT15, HT17, HT19, HT20, HT22, HT32, and HT59) isolated from Ixodes persulcatus adult ticks in Hokkaido, Japan, were selected and analyzed by polyacrylamide gel electrophoresis, with monoclonal and polyclonal antibodies, and by pulsed-field gel electrophoresis....

journal_title：Journal of clinical microbiology

authors： Fukunaga M,Sohnaka M,Takahashi Y,Nakao M,Miyamoto K

更新日期：1993-05-01 00:00:00

abstract：:There is a lack of consensus among investigators who use a variety of immunoassay techniques (e.g., enzyme-linked immunosorbent assay [ELISA] and radioimmunoassay) regarding the protocols for describing and forming standard reference or calibration curves and interpolating serum antibody concentrations. This confounds...

journal_title：Journal of clinical microbiology

authors： Plikaytis BD,Holder PF,Pais LB,Maslanka SE,Gheesling LL,Carlone GM

更新日期：1994-10-01 00:00:00

abstract：:A multilaboratory study designed to determine the in vitro susceptibility criteria and quality control parameters for ofloxacin against Neisseria gonorrhoeae was conducted according to the guidelines of the National Committee for Clinical Laboratory Standards. Proposed susceptibility breakpoints are MICs of less than ...

journal_title：Journal of clinical microbiology

authors： Fuchs PC,Barry AL,Baker C,Murray PR,Washington JA

更新日期：1992-04-01 00:00:00

abstract：:A study to compare the polymerase chain reaction (PCR) test with the cell culture method in diagnosing urogenital Chlamydia trachomatis infections was performed. From 497 patients (212 women, 285 men) attending an outpatient clinic for sexually transmitted diseases, a total of 814 samples (female patients, cervix and ...

journal_title：Journal of clinical microbiology

authors： Vogels WH,van Voorst Vader PC,Schröder FP

更新日期：1993-05-01 00:00:00

abstract：:The use of high-risk human papillomavirus (HPV) testing for surveillance and clinical applications is increasing globally, and it is important that tests are evaluated to ensure they are fit for this purpose. In this study, the performance of a new HPV genotyping test, the Papilloplex high-risk HPV (HR-HPV) test, was ...

journal_title：Journal of clinical microbiology

authors： Bhatia R,Serrano I,Wennington H,Graham C,Cubie H,Boland E,Fu G,Cuschieri K

更新日期：2018-02-22 00:00:00

abstract：:Recent molecular studies have shown Mycobacterium porcinum, recovered from cases of lymphadenitis in swine, to have complete 16S rDNA sequence identity and >70% DNA-DNA homology with human isolates within the M. fortuitum third biovariant complex. We identified 67 clinical and two environmental isolates of the M. fort...

journal_title：Journal of clinical microbiology

authors： Wallace RJ Jr,Brown-Elliott BA,Wilson RW,Mann L,Hall L,Zhang Y,Jost KC Jr,Brown JM,Kabani A,Schinsky MF,Steigerwalt AG,Crist CJ,Roberts GD,Blacklock Z,Tsukamura M,Silcox V,Turenne C

更新日期：2004-12-01 00:00:00

abstract：:The human erythrocyte receptor which mediates mannose-resistant hemagglutination by enterotoxigenic Escherichia coli possessing colonization factor antigen II is not universally distributed among donors. Mannose-resistant hemagglutination-positive erythrocytes are more common among black donors than nonblack donors; t...

journal_title：Journal of clinical microbiology

authors： Evans DJ Jr,Evans DG,Diaz SR,Graham DY

更新日期：1988-09-01 00:00:00

abstract：:Since the use of highly purified viral proteins in the solid-phase radioimmunoassay (SPRIA) procedure is not practical for routine diagnostic serology, the procedure was adapted for use with the crude antigens now commonly used to diagnose St. Louis encephalitis (SLE) virus infections. SLE and various other alpha- and...

journal_title：Journal of clinical microbiology

authors： Wolff KL,Muth DJ,Hudson BW,Trent DW

更新日期：1981-08-01 00:00:00

abstract：:Phosphatase activity was found to be applicable as a criterion for the differentiation of Mycoplasma salivarium and Mycoplasma orale, predominant constituents of oral mycoplasmal flora. Therefore, a simple procedure for the phosphatase activity assay was established as a screening test for the differentiation of oral ...

journal_title：Journal of clinical microbiology

authors： Shibata K,Totsuka M,Watanabe T

更新日期：1986-05-01 00:00:00

abstract：:More than 700 bacterial species or phylotypes, of which over 50% have not been cultivated, have been detected in the oral cavity. Our purposes were (i) to utilize culture-independent molecular techniques to extend our knowledge on the breadth of bacterial diversity in the healthy human oral cavity, including not-yet-c...

journal_title：Journal of clinical microbiology

authors： Aas JA,Paster BJ,Stokes LN,Olsen I,Dewhirst FE

更新日期：2005-11-01 00:00:00

abstract：:Invasive collection methods are often required to obtain samples for the microbiological evaluation of children with presumptive pulmonary tuberculosis (PTB). Nucleic acid amplification testing of easier-to-collect stool samples could be a noninvasive method of diagnosing PTB. We conducted a systematic review and meta...

journal_title：Journal of clinical microbiology

authors： MacLean E,Sulis G,Denkinger CM,Johnston JC,Pai M,Ahmad Khan F

更新日期：2019-05-24 00:00:00

abstract：:The effect of supplementing nutrient substrate with various combinations of concentrations of oleic and linoleic acids on the growth of 11 strains of Staphylococcus aureus was assessed. Whereas increasing the concentration of linoleic acid by itself greatly diminished the growth of all 11 strains, concomitant increase...

journal_title：Journal of clinical microbiology

authors： Campbell IM,Crozier DN,Pawagi AB,Buivids IA

更新日期：1983-08-01 00:00:00

abstract：:The DNA relatedness and restriction fragment length polymorphism patterns of whole-cell DNA and the general phenotypic properties of 14 isolates of the form species Candida parapsilosis representing three diverse genetic groups were compared. The data confirm the unrelatedness of the three groups at the species level....

journal_title：Journal of clinical microbiology

authors： Roy B,Meyer SA

更新日期：1998-01-01 00:00:00

abstract：:Twenty-three young adult rhesus monkeys from China were evaluated for the presence of Helicobacter pylori. Gastric body and antral biopsy samples were tested for H. pylori by PCR analysis, culture, rapid urease testing, and histologic evaluation. Serologic testing to detect H. pylori immunoglobulin G (IgG) antibodies ...

journal_title：Journal of clinical microbiology

authors： Handt LK,Fox JG,Yan LL,Shen Z,Pouch WJ,Ngai D,Motzel SL,Nolan TE,Klein HJ

更新日期：1997-01-01 00:00:00

abstract：:A PCR method for the unequivocal assignment of Haemophilus influenzae capsular type (types a to f) was developed. PCR primers were designed from capsule type-specific DNA sequences cloned from the capsular gene cluster of each of the six capsular types. PCR product was amplified only from the capsular type for which t...

journal_title：Journal of clinical microbiology

authors： Falla TJ,Crook DW,Brophy LN,Maskell D,Kroll JS,Moxon ER

更新日期：1994-10-01 00:00:00

abstract：:Post kala-azar dermal leishmaniasis (PKDL) is a skin manifestation that usually develops after treatment of visceral leishmaniasis (VL), a major public health problem in India. The diagnosis and management of PKDL is complex. This is the first case report from India in which PKDL occurred after paromomycin treatment f...

journal_title：Journal of clinical microbiology

authors： Pandey K,Das VN,Singh D,Das S,Lal CS,Verma N,Bimal S,Topno RK,Siddiqui NA,Verma RB,Sinha PK,Das P

更新日期：2012-04-01 00:00:00

abstract：:Dried blood spot (DBS) specimens were assessed as an alternative to plasma for human immunodeficiency virus type 1 (HIV-1) serotyping by V3 loop peptide enzyme immunoassay. Nested PCR capable of distinguishing HIV-1 subtypes B and E was used as the reference standard. Ninety-two percent of DBS samples were typeable as...

journal_title：Journal of clinical microbiology

authors： Chanbancherd P,Brown AE,Trichavaroj R,Tienamporn P,Puthakird P,Limpairojn N,VanCott TC,de Souza MS

更新日期：1999-03-01 00:00:00