Comparison of phenotypic and genotypic methods for detection of diphtheria toxin among isolates of pathogenic corynebacteria.

abstract：:We compared the MicroScan Pos MIC Type 6 panel and AMS-Vitek Gram Positive Susceptibility Card (GPS-TA) to agar dilution screen plates for the detection of high-level aminoglycoside resistance in 182 enterococcal isolates. The specificity of the two commercial systems was 100%, with the exception of one susceptible is...

journal_title：Journal of clinical microbiology

authors： Szeto S,Louie M,Low DE,Patel M,Simor AE

更新日期：1991-06-01 00:00:00

abstract：:The recent emergence of the multidrug-resistant and pathogenic yeast Candida auris continues to cause public health concern worldwide. C. auris is alarming because it causes health care-associated outbreaks and can establish invasive infections with high mortality rates. Transmission between patients is facilitated by...

journal_title：Journal of clinical microbiology

authors： Sexton DJ,Kordalewska M,Bentz ML,Welsh RM,Perlin DS,Litvintseva AP

更新日期：2018-11-27 00:00:00

abstract：:Nasopharyngeal secretions from adults and children were obtained in Stockholm, Sweden, for routine diagnosis of influenza A virus, influenza B virus, respiratory syncytial (RS) virus, parainfluenza type 3 virus, and adenovirus infections by demonstration of viral antigens directly in the specimens. The cells in nasoph...

journal_title：Journal of clinical microbiology

authors： Grandien M,Pettersson CA,Gardner PS,Linde A,Stanton A

更新日期：1985-11-01 00:00:00

abstract：:We report on a case of phaeohyphomycosis caused by Alternaria infectoria in a renal transplant recipient with pulmonary infiltrates and multiple skin lesions. Diagnosis was based on microscopy and culture of the skin lesions. Treatment consisted of a combination of surgical excision and systemic antifungal therapy, fi...

journal_title：Journal of clinical microbiology

authors： Halaby T,Boots H,Vermeulen A,van der Ven A,Beguin H,van Hooff H,Jacobs J

更新日期：2001-05-01 00:00:00

abstract：:Dogs orally infected with Neorickettsia helminthoeca developed immunoglobulin G titers against Erlichia risticii, Erlichia sennetsu, and Erlichia canis similar to those against N. helminthoeca antigen, as determined by immunofluorescence. Western immunoblotting showed that the major common antigens shared among the mi...

journal_title：Journal of clinical microbiology

authors： Rikihisa Y

更新日期：1991-09-01 00:00:00

abstract：:Sixty-six Corynebacterium diphtheriae strains (62 of the gravis biotype and 4 of the mitis biotype) isolated during the Georgian diphtheria epidemic of 1993 to 1998 and 13 non-Georgian C. diphtheriae strains (10 Russian and 3 reference isolates) were characterized by (i) biotyping, (ii) toxigenicity testing with the E...

journal_title：Journal of clinical microbiology

authors： Sulakvelidze A,Kekelidze M,Gomelauri T,Deng Y,Khetsuriani N,Kobaidze K,De Zoysa A,Efstratiou A,Morris JG Jr,Imnadze P

更新日期：1999-10-01 00:00:00

abstract：:Mycobacterium tuberculosis isolates were studied by comparing chromosomal DNA fingerprint patterns produced by digestion of chromosomal DNA with BamHI, followed by agarose electrophoresis and hybridization with radiolabeled probes of insertion sequence IS6110. DNA fingerprints of 14 isolates from separate members of f...

journal_title：Journal of clinical microbiology

authors： Mazurek GH,Cave MD,Eisenach KD,Wallace RJ Jr,Bates JH,Crawford JT

更新日期：1991-09-01 00:00:00

abstract：:Blood isolates of Salmonella enterica serovar Typhi from two recently returned Bangladeshi patients in Kuwait were ciprofloxacin resistant, with ciprofloxacin MICs of 12 mg/liter for both isolates. Both isolates had three novel gyrA mutations (55-Leu-->Trp, 87-Asp-->Ala, and 106-Gln-->Arg) and three novel parC mutatio...

journal_title：Journal of clinical microbiology

authors： Dimitrov T,Dashti AA,Albaksami O,Udo EE,Jadaon MM,Albert MJ

更新日期：2009-01-01 00:00:00

abstract：:Recovery of Mycobacterium tuberculosis from sputa treated with cetyl-pyridinium chloride (CPC) and stored for 20 +/- 9 days was significantly higher than that from sputa that were untreated and processed by the N-acetyl-L-cisteine-NaOH method. Addition of CPC is useful for isolation of M. tuberculosis from sputa subje...

journal_title：Journal of clinical microbiology

authors： Pardini M,Varaine F,Iona E,Arzumanian E,Checchi F,Oggioni MR,Orefici G,Fattorini L

更新日期：2005-01-01 00:00:00

abstract：:Acute and convalescent-phase sera obtained from 10 patients infected with a Japanese strain of spotted fever group (SFG) rickettsia were tested by the indirect immunoperoxidase test, the Weil-Felix test, an enzyme-linked immunosorbent assay (ELISA), and immunoblotting. By the Weil-Felix test, the reactivity of these s...

journal_title：Journal of clinical microbiology

authors： Amano K,Hatakeyama H,Okuta M,Suto T,Mahara F

更新日期：1992-09-01 00:00:00

abstract：:Performance in proficiency testing (PT) schemes is an objective measure of a laboratory's best performance. We examined the performance of participants in two parasitology PT schemes in South Africa from 2004 through 2010. The average rates of acceptable scores over the period were 58% and 66% for the stool and blood ...

journal_title：Journal of clinical microbiology

authors： Poonsamy B,Dini L,Frean J

更新日期：2012-10-01 00:00:00

abstract：:Genetic studies of serogroup 6 isolates ofStreptococcus pneumoniaeidentified putative serotype 6E. Although its capsular polysaccharide structure has not been elucidated, putative serotype 6E is described in an increasing number of studies as a potentially new serotype. We show here that SPEC6B, which is widely used a...

journal_title：Journal of clinical microbiology

authors： Burton RL,Geno KA,Saad JS,Nahm MH

更新日期：2016-04-01 00:00:00

abstract：:Enteroviruses have been reported in encephalitis cases. However, clinical and epidemiological characteristics of enteroviruses in encephalitis are not fully established. We prospectively investigated 204 children with encephalitis over a period of 2 years (2009 to 2010) for enterovirus. Enterovirus was detected in 45 ...

journal_title：Journal of clinical microbiology

authors： Kumar A,Shukla D,Kumar R,Idris MZ,Misra UK,Dhole TN

更新日期：2012-11-01 00:00:00

abstract：:In January 1991, an outbreak of cholera started in Peru and spread throughout most of Latin America within 8 months. As of March 1992, over 450,000 cases and approximately 4,000 deaths have been reported to the Pan American Health Organization. The causative organism is toxigenic Vibrio cholerae O1 of the El Tor bioty...

journal_title：Journal of clinical microbiology

authors： Fields PI,Popovic T,Wachsmuth K,Olsvik O

更新日期：1992-08-01 00:00:00

abstract：:The use of counterimmunoelectrophoresis (CIE) for detection of serum antibodies to staphylococcal teichoic acids was evaluated against teichoic acids prepared by sonic treatment or lysostaphin extraction of Staphylococcus aureus (Lafferty strain). Of 54 patient sera from suspected cases of staphylococcal endocarditis,...

journal_title：Journal of clinical microbiology

authors： Leffell MS,Folds JD,Wasilauskas B

更新日期：1978-11-01 00:00:00

abstract：:Although it has been recommended that photochromogenicity of mycobacteria should be tested after incubation at 25 to 30 degrees C for 2 weeks (Wayne et al., Int. J. Syst. Bacteriol. 24:412-419, 1974), our results indicate that photochromogenicity should be determined at 37 degrees C in the dark and under continuous ex...

journal_title：Journal of clinical microbiology

authors： Tsukamura M

更新日期：1981-08-01 00:00:00

abstract：:Molecular techniques are playing an important role in the diagnosis of nontuberculous mycobacterial infections. This case report describes a chronic soft tissue infection in an immunocompetent patient caused by a previously undescribed pigmented, rapidly growing Mycobacterium species, emphasizing the importance of cli...

journal_title：Journal of clinical microbiology

authors： Turenne CY,Suchak AA,Wolfe JN,Kabani A,Nicolle LE

更新日期：2003-06-01 00:00:00

abstract：:Automated DNA sequencing was used to characterize mutations associated with rifampin resistance in a 69-bp region of the gene, rpoB, encoding the beta subunit of RNA polymerase in Mycobacterium tuberculosis. The data confirmed that greater than 90% of rifampin-resistant strains have sequence alterations in this region...

journal_title：Journal of clinical microbiology

authors： Kapur V,Li LL,Iordanescu S,Hamrick MR,Wanger A,Kreiswirth BN,Musser JM

更新日期：1994-04-01 00:00:00

abstract：:Interferon gamma (IFN-γ) release assays (IGRAs) are increasingly used to test for latent tuberculosis (TB) infection. Although highly specific, IGRAs have a relatively high false-negative rate in active TB patients. A more sensitive assay is needed. IFN-γ-induced protein 10 (IP-10) is an alternative biomarker with a 1...

journal_title：Journal of clinical microbiology

authors： Blauenfeldt T,Villar-Hernández R,García-García E,Latorre I,Holm LL,Muriel-Moreno B,De Souza-Galvão ML,Millet JP,Sabriá F,Sánchez-Montalva A,Ruiz-Manzano J,Pilarte J,Jiménez MA,Centeno C,Torres C,Molina-Pinargote I,Gonzále

更新日期：2020-09-22 00:00:00

abstract：:Bacillus anthracis spores have been used as a biological weapon in the United States. We wanted to develop a safe, rapid method of sample preparation that provided safe DNA for the detection of spores in environmental and clinical specimens. Our method reproducibly detects B. anthracis in samples containing <10 spores...

journal_title：Journal of clinical microbiology

authors： Luna VA,King D,Davis C,Rycerz T,Ewert M,Cannons A,Amuso P,Cattani J

更新日期：2003-03-01 00:00:00

abstract：:The performance of the BDProbeTec ET system (BD Biosciences, Sparks, Md.) for direct detection of Mycobacterium tuberculosis complex (MTBC) in respiratory specimens was evaluated by comparing results to those of conventional mycobacterial culture performed with the BACTEC 460 TB system and Middlebrook 7H11 biplates. P...

journal_title：Journal of clinical microbiology

authors： Bergmann JS,Keating WE,Woods GL

更新日期：2000-02-01 00:00:00

abstract：:The genetic composition and antibiotic sensitivities of 50 clinical isolates of Staphylococcus aureus obtained from various clinics in the Republic of Georgia were characterized. S. aureus strains ATCC 700699 and ATCC 29737 were included as reference standards in all analyses. All 52 strains had identical 16S rRNA pro...

journal_title：Journal of clinical microbiology

authors： Revazishvili T,Bakanidze L,Gomelauri T,Zhgenti E,Chanturia G,Kekelidze M,Rajanna C,Kreger A,Sulakvelidze A

更新日期：2006-10-01 00:00:00

abstract：:Nocardia cyriacigeorgica is a recently characterized species within the genus of Nocardia. We report a brain abscess, following a primary pulmonary colonization, due to this species in a human immunodeficiency virus-infected patient. This case confirms that isolation of Nocardia in sputum is associated with a high ris...

journal_title：Journal of clinical microbiology

authors： Barnaud G,Deschamps C,Manceron V,Mortier E,Laurent F,Bert F,Boiron P,Vinceneux P,Branger C

更新日期：2005-09-01 00:00:00

abstract：:Fresh urine pellets from human immunodeficiency virus type 1 (HIV-1)-seropositive individuals were examined for the presence of the HIV-1 genomic sequence and gene products. By using the polymerase chain reaction technique, HIV-1 DNA proviral sequences were detected in 53 of 80 (66.25%) fresh urine pellets from HIV-1-...

journal_title：Journal of clinical microbiology

authors： Li JJ,Huang YQ,Poiesz BJ,Zaumetzger-Abbot L,Friedman-Kien AE

更新日期：1992-05-01 00:00:00

abstract：:We assessed neutralizing antibody titers to adenovirus serotype 5 (Ad5) and six rare adenovirus serotypes, serotypes 11, 35, 50, 26, 48, and 49, in pediatric populations in sub-Saharan Africa. We observed a clear age dependence of Ad5-specific neutralizing antibody titers. These data will help to guide the development...

journal_title：Journal of clinical microbiology

authors： Thorner AR,Vogels R,Kaspers J,Weverling GJ,Holterman L,Lemckert AA,Dilraj A,McNally LM,Jeena PM,Jepsen S,Abbink P,Nanda A,Swanson PE,Bates AT,O'Brien KL,Havenga MJ,Goudsmit J,Barouch DH

更新日期：2006-10-01 00:00:00

abstract：:A multiplex method using the SNaPshot technique was developed to screen for six common mycobacterial species: Mycobacteria tuberculosis, M. avium, M. intracellulare, M. chelonae, M. kansasii, and M. gordonae. A total of 468 mycobacterial clinical isolates were subjected to analysis for the presence of the six mycobact...

journal_title：Journal of clinical microbiology

authors： Wang H,Yue J,Han M,Yang J,Zhao Y

更新日期：2010-01-01 00:00:00

abstract：:During the last 10 to 15 years, Aerococcus-like organisms have been isolated from urinary tract specimens from hospitalized patients at Bispebjerg Hospital, Copenhagen, Denmark, with signs of urinary tract infection, and a single strain has been deposited at the National Collection of Type Cultures (NCTC 12142). An ad...

journal_title：Journal of clinical microbiology

authors： Christensen JJ,Vibits H,Ursing J,Korner B

更新日期：1991-05-01 00:00:00

abstract：:The recovery of Mycobacterium tuberculosis from sputa positive or negative for acid-fast bacilli that were stored for 17 +/- 7 days and inoculated in the BACTEC MGIT 960 system (MGIT) was higher than that from sputa inoculated in Lowenstein-Jensen medium. MGIT is useful for isolation of M. tuberculosis from sputa subj...

journal_title：Journal of clinical microbiology

authors： Pardini M,Varaine F,Bonnet M,Orefici G,Oggioni MR,LONG-DRUG Study Group.,Fattorini L

更新日期：2007-02-01 00:00:00

abstract：:Screening of blood for human T-cell lymphotropic virus type 1 and type 2 (HTLV-1 and -2, respectively) is important to diagnose and prevent infection and ensure the safety of blood supplies. The Elecsys HTLV-I/II assay is a newly developed, electrochemiluminescence screening assay for the detection of HTLV-1/2 infecti...

journal_title：Journal of clinical microbiology

authors： Laperche S,Sauleda S,Piron M,Mühlbacher A,Schennach H,Schottstedt V,Queirós L,Uno N,Yanagihara K,Imdahl R,Hey A,Klinkicht M,Melchior W,Muench P,Watanabe T

更新日期：2017-07-01 00:00:00

abstract：:Genetic determinants of the invasive phenotype of Shigella spp. and enteroinvasive Escherichia coli (EIEC), two common agents of bacillary dysentery, are encoded on large (180- to 210 kilobase), nonconjugative plasmids. Several plasmid-encoded antigens have been implicated as important bacterial ligands that mediate t...

journal_title：Journal of clinical microbiology

authors： Venkatesan M,Buysse JM,Vandendries E,Kopecko DJ

更新日期：1988-02-01 00:00:00