Epidemiological study of a food-borne outbreak of enterotoxigenic Escherichia coli O25:NM by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA analysis.

abstract：:The epidemiology of hospital infections due to Pseudomonas aeruginosa was investigated by pyocin typing. The typing method, which determined the pyocin activity of clinical isolates of P. aeruginosa on 27 indicator strains, was 43.7% reproducible, but elimination of 9 indicator strains doubled the reproducibility and ...

journal_title：Journal of clinical microbiology

authors： Bruun FN,McGarrity GJ,Blakemore WS,Coriell LL

更新日期：1976-03-01 00:00:00

abstract：:Pneumococcus (Streptococcus pneumoniae) remains a significant cause of morbidity and mortality, especially among those at the extremes of age. Its capsular polysaccharide is essential for systemic virulence. Over 90 serologically distinct pneumococcal capsular polysaccharides (serotypes) are recognized, but they are u...

journal_title：Journal of clinical microbiology

authors： Geno KA,Saad JS,Nahm MH

更新日期：2017-05-01 00:00:00

abstract：:Group A streptococcus (GAS) species cause bacterial pharyngitis in both adults and children. Early and accurate diagnosis of GAS is important for appropriate antibiotic therapy to prevent GAS sequalae. The Revogene Strep A molecular assay (Meridian Bioscience Canada Inc, Quebec City, QC, Canada) is an automated real-t...

journal_title：Journal of clinical microbiology

authors： Banerjee D,Michael J,Schmitt B,Salimnia H,Mhaissen N,Goldfarb DM,Lachance P,Faron ML,Aufderheide T,Ledeboer N,Weissfeld A,Selvarangan R

更新日期：2020-06-24 00:00:00

abstract：:We report a novel IS711 chromosomal location that is specific for the Brucella genotype ST27 previously associated with Pacific marine mammals and human zoonotic infection in New Zealand and Peru. Our data support the previous observation that this peculiar genotype is distinct from those commonly isolated from the At...

journal_title：Journal of clinical microbiology

authors： Cloeckaert A,Bernardet N,Koylass MS,Whatmore AM,Zygmunt MS

更新日期：2011-11-01 00:00:00

abstract：:Sapporo-like viruses (SLVs) are associated with acute gastroenteritis in humans. Due to a limited supply of available reagents for diagnosis, little is known about the incidence and pathogenicity of these viruses. We have developed a first-generation generic reverse transcriptase (RT) PCR assay based on a single prime...

journal_title：Journal of clinical microbiology

authors： Vinjé J,Deijl H,van der Heide R,Lewis D,Hedlund KO,Svensson L,Koopmans MP

更新日期：2000-02-01 00:00:00

abstract：:Sequence analysis of an 850-bp fragment internal to the aspecific drug efflux gene ade B revealed 11 sequence types (STs) among a collection of 50 multidrug-resistant Acinetobacter baumannii (MDRAB) strains, including members of pan-European clones I, II, and III. The delineation of STs conformed with the intraspecifi...

journal_title：Journal of clinical microbiology

authors： Huys G,Cnockaert M,Nemec A,Swings J

更新日期：2005-10-01 00:00:00

abstract：:The performance of a commercially available, rapid membrane enzyme immunoassay for influenza A and B virus detection was compared to that of viral culture in 4,092 respiratory specimens collected from patients presenting with respiratory symptoms during the 2002-2003 influenza season. The test's overall sensitivity wa...

journal_title：Journal of clinical microbiology

authors： Cazacu AC,Chung SE,Greer J,Demmler GJ

更新日期：2004-08-01 00:00:00

abstract：:Ureaplasma urealyticum has been associated with urethritis in men, obstetric problems in women, and respiratory distress syndrome in preterm infants. U. urealyticum can be divided into two biovars comprising 14 serovars. Partial sequences of genes encoding the multiple-banded antigens of the cell surface are known. Us...

journal_title：Journal of clinical microbiology

authors： Pitcher D,Sillis M,Robertson JA

更新日期：2001-05-01 00:00:00

abstract：:A sensitive PCR assay that detects the thermophilic campylobacters C. jejuni, C. coli, C. lari, and C. upsaliensis is reported. Furthermore, by digestion of the PCR products with two restriction enzymes, species differentiation was demonstrated. Thus, the present method has the potential to be used for both detection ...

journal_title：Journal of clinical microbiology

authors： Fermér C,Engvall EO

更新日期：1999-10-01 00:00:00

abstract：:A novel reverse primer (GLM MR1) was designed for detection of the glmM gene in Helicobacter pylori by PCR. The percentage of amplification in clinical isolates using GLM MR1 was 100% for detection of the glmM gene and 86.36% for the ureA gene. The primer designed is useful for the identification of H. pylori. ...

journal_title：Journal of clinical microbiology

authors： Espinoza MG,Vazquez RG,Mendez IM,Vargas CR,Cerezo SG

更新日期：2011-04-01 00:00:00

abstract：:We examined an antiserum agar method to study its reliability in screening Staphylococcus aureus strains for capsule production. The encapsulated S. aureus Smith diffuse strain was compared with its nonencapsulated variant, Smith compact, in CCY medium containing 0.5% NaCl and 5.0% Smith diffuse rabbit antiserum. A ha...

journal_title：Journal of clinical microbiology

authors： West TE,Apicella MA

更新日期：1984-08-01 00:00:00

abstract：:Chronic respiratory tract infections caused by Staphylococcus aureus are common in patients with cystic fibrosis (CF). Recently, it was shown in a few CF patients that S. aureus isolates produce capsular polysaccharides (CPs). However, it is not known whether this is a common feature and whether an immune response to ...

journal_title：Journal of clinical microbiology

authors： Albus A,Fournier JM,Wolz C,Boutonnier A,Ranke M,Høiby N,Hochkeppel H,Döring G

更新日期：1988-12-01 00:00:00

abstract：:The enzyme-linked immunosorbent assay (ELISA) has been proposed as an alternative serologic diagnostic test to the indirect immunofluorescence assay (IFA) for scrub typhus. Here, we systematically determine the optimal sample dilution and cutoff optical density (OD) and estimate the accuracy of IgM ELISA using Bayesia...

journal_title：Journal of clinical microbiology

authors： Blacksell SD,Lim C,Tanganuchitcharnchai A,Jintaworn S,Kantipong P,Richards AL,Paris DH,Limmathurotsakul D,Day NPJ

更新日期：2016-06-01 00:00:00

abstract：:We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to individual testing, ...

journal_title：Journal of clinical microbiology

authors： Edouard S,Prudent E,Gautret P,Memish ZA,Raoult D

更新日期：2015-03-01 00:00:00

abstract：:BBL CHROMagar VanRE (CVRE) was compared with bile esculin azide agar plus vancomycin to screen for vancomycin-resistant enterococcus (VRE) colonization. CVRE distinguishes Enterococcus faecalis (green colonies) from Enterococcus faecium (mauve colonies) on the basis of chromogenic substrate use. CVRE sensitivity and s...

journal_title：Journal of clinical microbiology

authors： Kallstrom G,Doern CD,Dunne WM Jr

更新日期：2010-03-01 00:00:00

abstract：:The main goal of this study was to determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA), particularly livestock-associated MRSA (LA-MRSA) in pigs and pork. The genotypic relatedness of isolates on the farm, at slaughter, and at the retail level was assessed. Paired nasal and perianal swab sa...

journal_title：Journal of clinical microbiology

authors： Molla B,Byrne M,Abley M,Mathews J,Jackson CR,Fedorka-Cray P,Sreevatsan S,Wang P,Gebreyes WA

更新日期：2012-11-01 00:00:00

abstract：:A simple, rapid procedure to separate Pneumocystis carinii obtained by lavage of lungs of steroid-treated rats from rat leukocytes is described. Commercially available monoclonal antibody to rat common leukocyte determinants is used to sediment the leukocytes, resulting in supernatant fluid containing P. carinii cells...

journal_title：Journal of clinical microbiology

authors： Pesanti EL,McCarron BA

更新日期：1988-08-01 00:00:00

abstract：:Enterocytozoon bieneusi, a microsporidian parasite, has been recognized since 1985 as an agent of intestinal microsporidiosis leading to malabsorption syndrome, diarrhea, and weight loss in AIDS patients. Recently, however, we have identified E. bieneusi spores in the sputum, bronchoalveolar lavage, and stool samples ...

journal_title：Journal of clinical microbiology

authors： del Aguila C,Lopez-Velez R,Fenoy S,Turrientes C,Cobo J,Navajas R,Visvesvara GS,Croppo GP,Da Silva AJ,Pieniazek NJ

更新日期：1997-07-01 00:00:00

abstract：:Microsporidia are protozoa parasites responsible for significant gastrointestinal disease in patients infected with human immunodeficiency virus. We evaluated a PCR assay of stool samples, duodenal aspirates, and biopsy specimens from patients with Enterocytozoon bieneusi infection. A 210-bp DNA fragment of the unique...

journal_title：Journal of clinical microbiology

authors： Velásquez JN,Carnevale S,Guarnera EA,Labbé JH,Chertcoff A,Cabrera MG,Rodríguez MI

更新日期：1996-12-01 00:00:00

abstract：:Automated treponemal immunoassays are used for syphilis screening with the reverse-sequence algorithm; discordant results (e.g., enzyme immunoassay [EIA] reactive and reactive plasma reagin [RPR] nonreactive) are resolved with a second treponemal test. We conducted a study to determine automated immunoassay signal str...

journal_title：Journal of clinical microbiology

authors： Fakile YF,Jost H,Hoover KW,Gustafson KJ,Novak-Weekley SM,Schapiro JM,Tran A,Chow JM,Park IU

更新日期：2017-12-26 00:00:00

abstract：:Sixty-six Corynebacterium diphtheriae strains (62 of the gravis biotype and 4 of the mitis biotype) isolated during the Georgian diphtheria epidemic of 1993 to 1998 and 13 non-Georgian C. diphtheriae strains (10 Russian and 3 reference isolates) were characterized by (i) biotyping, (ii) toxigenicity testing with the E...

journal_title：Journal of clinical microbiology

authors： Sulakvelidze A,Kekelidze M,Gomelauri T,Deng Y,Khetsuriani N,Kobaidze K,De Zoysa A,Efstratiou A,Morris JG Jr,Imnadze P

更新日期：1999-10-01 00:00:00

abstract：:Borrelia lusitaniae is a species within the complex Borrelia burgdorferi sensu lato and is infrequently isolated in Europe. In contrast, this species is by far the most predominant in North Africa and in Portugal. In this study, we analyzed the genetic diversity, at several loci, of a large population of isolates from...

journal_title：Journal of clinical microbiology

authors： Younsi H,Sarih M,Jouda F,Godfroid E,Gern L,Bouattour A,Baranton G,Postic D

更新日期：2005-04-01 00:00:00

abstract：:Antibody responses of 85 patients to human immunodeficiency virus type 1 antigens were quantitated by densitometric analysis of Western blot (immunoblot) assays. All patients had been classified into the following three clinical categories: asymptomatic (ASY), acquired immunodeficiency syndrome (AIDS)-related complex ...

journal_title：Journal of clinical microbiology

authors： Schmidt G,Amiraian K,Frey H,Wethers J,Stevens RW,Berns DS

更新日期：1989-05-01 00:00:00

abstract：:Screening for Chlamydia trachomatis infection in men has traditionally been limited to men who present with urethral symptoms, thereby limiting the detection of asymptomatic chlamydia infection in men. In order to effectively screen both symptomatic and asymptomatic men, we evaluated a newly developed polymerase chain...

journal_title：Journal of clinical microbiology

authors： Jaschek G,Gaydos CA,Welsh LE,Quinn TC

更新日期：1993-05-01 00:00:00

abstract：:Salmonella typhi was isolated from 369 and Salmonella paratyphi A was isolated from 6 of 515 Vietnamese patients with suspected enteric fever. Compared with conventional broth culture of blood, direct plating of the buffy coat had a diagnostic sensitivity of 99.5% (95% confidence interval [CI], 97.1 to 100%). Blood ba...

journal_title：Journal of clinical microbiology

authors： Wain J,Diep TS,Ho VA,Walsh AM,Nguyen TT,Parry CM,White NJ

更新日期：1998-06-01 00:00:00

abstract：:Gram-negative, anaerobic bacilli are unusual organisms to be isolated in cases of acute septic arthritis. We report the isolation of Fusobacterium necrophorum from joint aspirate in a case of acute septic arthritis, which presented 3 weeks after the drainage of a dental abscess. ...

journal_title：Journal of clinical microbiology

authors： Sonsale PD,Philipson MR,Bowskill J

更新日期：2004-07-01 00:00:00

abstract：:A commercial multiplex PCR (hyplex SuperBug ID) was tested with a collection of 132 clinical Enterobacteriaceae strains producing different carbapenemases. The sensitivity for the detection of KPC-, VIM-, NDM-, and OXA-48-encoding genes was 100%, whereas two IMP variants were missed. ...

journal_title：Journal of clinical microbiology

authors： Kaase M,Szabados F,Wassill L,Gatermann SG

更新日期：2012-09-01 00:00:00

abstract：:Explanted orthopedic implants from 54 patients with aseptic failure and 24 patients with prosthetic knee or hip infection were sonicated in polyethylene bags. The sensitivities of periprosthetic tissue and sonicate fluid cultures for the diagnosis of prosthetic joint infection were 54% and 75%, whereas the specificiti...

journal_title：Journal of clinical microbiology

authors： Trampuz A,Piper KE,Hanssen AD,Osmon DR,Cockerill FR,Steckelberg JM,Patel R

更新日期：2006-02-01 00:00:00

abstract：:We determined the E-Test and National Committee for Clinical Laboratory Standards standardized agar dilution MICs of ceftazidime, ciprofloxacin, piperacillin, and tobramycin for Pseudomonas aeruginosa during tests of 100 rough and mucoid P. aeruginosa isolates from cystic fibrosis patients. The levels of agreement (+/...

journal_title：Journal of clinical microbiology

authors： Marley EF,Mohla C,Campos JM

更新日期：1995-12-01 00:00:00

abstract：:An automated cultivation system for mycobacteria, the MGIT 960 system (MGIT system), was compared in the clinical routine with two variants of Löwenstein-Jensen (L-J) medium. A total of 152 isolates were recovered from 2,015 specimens: 139 (91%) with the MGIT system and 127 (84%) with L-J media (P = 0.05). These inclu...

journal_title：Journal of clinical microbiology

authors： Katila ML,Katila P,Erkinjuntti-Pekkanen R

更新日期：2000-03-01 00:00:00