Diversity among community isolates of methicillin-resistant Staphylococcus aureus in Australia.

abstract：:Although screening for maternal toxoplasmic seroconversion during pregnancy is based on immunodiagnostic assays, the diagnosis of clinically relevant toxoplasmosis greatly relies upon molecular methods. A problem is that this molecular diagnosis is subject to variation of performances, mainly due to a large diversity ...

journal_title：Journal of clinical microbiology

authors： Sterkers Y,Varlet-Marie E,Cassaing S,Brenier-Pinchart MP,Brun S,Dalle F,Delhaes L,Filisetti D,Pelloux H,Yera H,Bastien P

更新日期：2010-09-01 00:00:00

abstract：:Immune pressure on lentiviruses exerted by cytotoxic T lymphocytes (CTL) selects for virus CTL epitope mutations. Currently employed methods for monitoring emerging CTL epitope mutations rely on the labor-intensive and time-consuming techniques of virus population or clonal sequencing. Here we describe the development...

journal_title：Journal of clinical microbiology

authors： Peyerl FW,Barouch DH,Bazick HS,Manuel E,Letvin NL

更新日期：2005-09-01 00:00:00

abstract：:Enterobacteriaceae represent a diverse and medically important family of bacteria that are difficult to identify to the species level using the standard molecular method of 16S rRNA gene sequencing. Prior work has demonstrated the value of dnaJ gene sequence analysis in resolving different members of the family. Howev...

journal_title：Journal of clinical microbiology

authors： McLean K,Rosenthal CA,Sengupta D,Owens J,Cookson BT,Hoffman NG,Salipante SJ

更新日期：2019-10-23 00:00:00

abstract：:A rapid colorimetric method for the identification of pathogenic Neisseria (Identicult-Neisseria; Scott Laboratories, Inc.) based on beta-galactosidase, gamma-glutamylaminopeptidase, and gamma-prolylaminopeptidase is described. All 82 clinical isolates of Neisseria gonorrhoeae, 9 clinical isolates of N. meningitidis, ...

journal_title：Journal of clinical microbiology

authors： Hosmer ME,Cohenford MA,Ellner PD

更新日期：1986-07-01 00:00:00

abstract：:Automation of the clinical microbiology laboratory has become more prominent as laboratories face higher specimen volumes and understaffing and are becoming more consolidated. One recent advancement is the use of digital image analysis to rapidly distinguish between chromogenic growth for screening bacterial cultures....

journal_title：Journal of clinical microbiology

authors： Faron ML,Buchan BW,Relich RF,Clark J,Ledeboer NA

更新日期：2020-03-25 00:00:00

abstract：:We evaluated the Phoenix automated microbiology system (BD Diagnostic Systems, Sparks, MD) for the identification (ID) and antimicrobial susceptibility testing (AST) of challenge and clinical staphylococci and enterococci recovered from patients in a tertiary-care medical center. In total, 424 isolates were tested: 90...

journal_title：Journal of clinical microbiology

authors： Carroll KC,Borek AP,Burger C,Glanz B,Bhally H,Henciak S,Flayhart DC

更新日期：2006-06-01 00:00:00

abstract：:Hepatitis E virus (HEV) genotype 3 is endemic in Europe and hyperendemic in southern France. Recent reports of a high prevalence of HEV RNA in blood donations and in culinary specialties from this geographical area confirmed the endemicity of HEV and sources of viral transmission in this geographical area. HEV causes ...

journal_title：Journal of clinical microbiology

authors： Moal V,Legris T,Motte A,Vacher-Coponat H,Fages L,Jourde-Chiche N,Borentain P,Jaubert D,Gerolami R,Colson P

更新日期：2015-05-01 00:00:00

abstract：:We undertook a controlled study of children younger than 5 years in Bangladesh to determine whether enterotoxigenic Bacteroides fragilis (ETBF) was associated with diarrhea in this population. ETBF was isolated from 22 (6.1%) of 358 patients and 5 (1.2%) of 425 controls (P = 0.0001). In children younger than 1 year, h...

journal_title：Journal of clinical microbiology

authors： Sack RB,Albert MJ,Alam K,Neogi PK,Akbar MS

更新日期：1994-04-01 00:00:00

abstract：:A rapid and sensitive urease test for mycobacteria utilizing a cumulative radiometric technique is described. Definitive results were obtained within a 30-min incubation period. The procedure is simple and economical. Technical time involved is no greater than in conventional procedures. ...

journal_title：Journal of clinical microbiology

authors： Cox FR,Cox ME,Martin JR

更新日期：1977-06-01 00:00:00

abstract：:Variable-number tandem repeats (VNTRs) may evolve so rapidly that multiple profiles emerge during an outbreak. A total of 190 isolates from eight Salmonella enterica serovar Typhimurium outbreaks and 15 isolates from seven patients were analyzed by pulsed-field gel electrophoresis and VNTR typing. Small changes in loc...

journal_title：Journal of clinical microbiology

authors： Hopkins KL,Maguire C,Best E,Liebana E,Threlfall EJ

更新日期：2007-09-01 00:00:00

abstract：:The genetic and phylogenetic characteristics of human enterovirus 71 (EV71) and coxsackievirus A16 (CA16) sampled from children with hand, foot, and mouth disease in Shenzhen, People's Republic of China, over a 6-year period (1999 to 2004) were examined with reverse transcription-PCR and DNA sequencing. Out of 147 sto...

journal_title：Journal of clinical microbiology

authors： Li L,He Y,Yang H,Zhu J,Xu X,Dong J,Zhu Y,Jin Q

更新日期：2005-08-01 00:00:00

abstract：:To detect Streptococcus pneumoniae colonization, the nasopharynx is sampled using a swab placed in skim milk-tryptone-glucose-glycerol (STGG) transport medium, and then the swab specimen or STGG medium is cultured or subjected to PCR. We evaluated the effect of swab composition and compared the sensitivities of detect...

journal_title：Journal of clinical microbiology

authors： Rubin LG,Rizvi A,Baer A

更新日期：2008-08-01 00:00:00

abstract：:Clonal spread and horizontal transfer in the spread of vancomycin resistance genes were investigated. Multiplex PCR, pulsed-field gel electrophoresis (PFGE), hybridization of enterococcal plasmids with the vanA and vanB probes, and sequencing of a fragment of vanB were used in the analysis. Before May 1996, 12 vancomy...

journal_title：Journal of clinical microbiology

authors： Suppola JP,Kolho E,Salmenlinna S,Tarkka E,Vuopio-Varkila J,Vaara M

更新日期：1999-12-01 00:00:00

abstract：:One hundred ninety-three Streptococcus agalactiae isolates of neonatal origin and 146 isolates from adult women were analyzed for macrolide resistance and investigated for clonality. Among erythromycin-resistant isolates, serotype V turned out to be the most frequent. Comparative pulsed-field gel electrophoresis analy...

journal_title：Journal of clinical microbiology

authors： von Both U,Ruess M,Mueller U,Fluegge K,Sander A,Berner R

更新日期：2003-05-01 00:00:00

abstract：:At the time indwelling urinary catheters were removed, the urethral flora was sampled by rolling the catheters onto culture plates. Cultures were obtained from 398 patients with sterile urine. Catheter surfaces were colonized less often in males than in females (16.8 and 67.0%, respectively) and yielded correspondingl...

journal_title：Journal of clinical microbiology

authors： Kunin CM,Steele C

更新日期：1985-06-01 00:00:00

abstract：:We prospectively investigated the diagnostic utility of the Xpert MTB/RIF (Mycobacterium tuberculosis/rifampin [RIF] resistance) assay in 20 cases with confirmed tuberculous pleural effusion. The sensitivity and specificity of the Xpert assay in pleural fluid were 25% and 100%, respectively. All cases positive by the ...

journal_title：Journal of clinical microbiology

authors： Friedrich SO,von Groote-Bidlingmaier F,Diacon AH

更新日期：2011-12-01 00:00:00

abstract：:Invasive aspergillosis (IA) is a life-threatening infection that mainly occurs in immunocompromised patients. Here, we compared the novel Aspergillus-specific galactomannoprotein (GP) enzyme-linked immunosorbent assay (ELISA) (Euroimmun Medizinische Labordiagnostika AG) to the established Platelia Aspergillus galactom...

journal_title：Journal of clinical microbiology

authors： Dichtl K,Seybold U,Ormanns S,Horns H,Wagener J

更新日期：2019-06-25 00:00:00

abstract：:Candida parapsilosis is an increasing cause of bloodstream infections (BSIs) in neonatal intensive care units (NICUs). It has been a persistent problem in the NICU of Hospital for Children and Adolescents, Helsinki University Central Hospital, Helsinki, Finland, since 1987. Fluconazole prophylaxis has been used to con...

journal_title：Journal of clinical microbiology

authors： Sarvikivi E,Lyytikäinen O,Soll DR,Pujol C,Pfaller MA,Richardson M,Koukila-Kähkölä P,Luukkainen P,Saxén H

更新日期：2005-06-01 00:00:00

abstract：:Epstein-Barr virus (EBV) infection is associated with a broad spectrum of disease. While quantification of EBV nucleic acid in the peripheral blood has been demonstrated to be useful for diagnosis and patient care, the optimal sample type and reporting format for such testing remain uncertain. Using quantitative real-...

journal_title：Journal of clinical microbiology

authors： Hakim H,Gibson C,Pan J,Srivastava K,Gu Z,Bankowski MJ,Hayden RT

更新日期：2007-07-01 00:00:00

abstract：:Childhood tuberculosis (TB) presents significant diagnostic challenges associated with paucibacillary disease and requires a more sensitive test. We evaluated the diagnostic accuracy of Xpert MTB/RIF Ultra (Ultra) compared to other microbiological tests using respiratory samples from Ugandan children in the SHINE tria...

journal_title：Journal of clinical microbiology

authors： Ssengooba W,Iragena JD,Nakiyingi L,Mujumbi S,Wobudeya E,Mboizi R,Boulware D,Meya DB,Choo L,Crook AM,Lebeau K,Joloba M,Demers AM,Cresswell FV,Gibb DM

更新日期：2020-08-24 00:00:00

abstract：:The prevalence of tuberculosis continues to be high, and nontuberculous mycobacterial (NTM) infection has also emerged worldwide. Moreover, differential and accurate identification of mycobacteria to the species or subspecies level is an unmet clinical need. Here, we developed a one-step multiplex PCR assay using whol...

journal_title：Journal of clinical microbiology

authors： Chae H,Han SJ,Kim SY,Ki CS,Huh HJ,Yong D,Koh WJ,Shin SJ

更新日期：2017-09-01 00:00:00

abstract：:The DNA hybridization assay for genes encoding for Escherichia coli enterotoxins was used to examine water specimens in Thailand. In a reconstruction experiment, the DNA hybridization assay was 10(4) times more sensitive than testing random E. coli in the Y-1 adrenal and suckling mouse assays in identifying enterotoxi...

journal_title：Journal of clinical microbiology

authors： Echeverria P,Seriwatana J,Chityothin O,Chaicumpa W,Tirapat C

更新日期：1982-12-01 00:00:00

abstract：:The Lyme disease-associated spirochete, Borrelia burgdorferi, is maintained in enzootic cycles involving Ixodes ticks and small mammals. Previous studies demonstrated that B. burgdorferi expresses outer surface protein A (OspA) but not OspC when residing in the midgut of unfed ticks. However, after ticks feed on blood...

journal_title：Journal of clinical microbiology

authors： Schwan TG,Piesman J

更新日期：2000-01-01 00:00:00

abstract：:A blind volunteer was inoculated in one eye with an isolate of Chlamydia trachomatis in 1961 and followed for 20 years. During this time, many observations were made of his clinical responses to the first inoculation and several subsequent inoculations with the same and other strains, chlamydial shedding, and antibody...

journal_title：Journal of clinical microbiology

authors： Hanna L,Jawetz E,Dawson CR,Thygeson P

更新日期：1982-11-01 00:00:00

abstract：:The performance of a commercially available, rapid membrane enzyme immunoassay for influenza A and B virus detection was compared to that of viral culture in 4,092 respiratory specimens collected from patients presenting with respiratory symptoms during the 2002-2003 influenza season. The test's overall sensitivity wa...

journal_title：Journal of clinical microbiology

authors： Cazacu AC,Chung SE,Greer J,Demmler GJ

更新日期：2004-08-01 00:00:00

abstract：:Rotavirus serotype G9 is recognized as the most widespread of the emerging serotypes, emerging since 1996 as a frequent cause of severe acute gastroenteritis in children from many countries covering all continents of the world. This study characterized serotype G9 strains collected in three widely separated Australian...

journal_title：Journal of clinical microbiology

authors： Kirkwood C,Bogdanovic-Sakran N,Palombo E,Masendycz P,Bugg H,Barnes G,Bishop R

更新日期：2003-08-01 00:00:00

abstract：:This study evaluated the performance of the Rapidec Carba NP test, which was introduced recently into the market for the detection of carbapenemase production in a broad spectrum of β-lactamase-producing Enterobacteriaceae clinical isolates. In total, 252 clinical Enterobacteriaceae isolates that had been genetically ...

journal_title：Journal of clinical microbiology

authors： Hombach M,von Gunten B,Castelberg C,Bloemberg GV

更新日期：2015-12-01 00:00:00

abstract：:The Abbott RealTime MTB (RT MTB) assay is a new automated nucleic acid amplification test for the detection of Mycobacterium tuberculosis complex (MTBC) in clinical specimens. In combination with the RealTime MTB INH/RIF (RT MTB INH/RIF) resistance assay, which can be applied to RT MTB-positive specimens as an add-on ...

journal_title：Journal of clinical microbiology

authors： Hofmann-Thiel S,Molodtsov N,Antonenka U,Hoffmann H

更新日期：2016-12-01 00:00:00

abstract：:Increasing frequencies of community-acquired methicillin-resistant Staphylococcus aureus (MRSA) strain isolation have been reported from many countries. The overall prevalence of MRSA in Norway is still very low. MRSA isolates (n = 67) detected between 1995 and 2003 in northern Norway were analyzed by pulsed-field gel...

journal_title：Journal of clinical microbiology

authors： Hanssen AM,Fossum A,Mikalsen J,Halvorsen DS,Bukholm G,Sollid JU

更新日期：2005-05-01 00:00:00

abstract：:This study determined the group A rotavirus occurrence in pig farms from 7 different cities in São Paulo State, Brazil. Out of 143 samples, 70 tested positive. Sequence analyses of 37 strains indicated that the strains had the G3, G5, G9, and P[6], P[13]/P[22]-like, and P[23] genotypes. ...

journal_title：Journal of clinical microbiology

authors： Tonietti PO,Hora AS,Silva FD,Ruiz VL,Gregori F

更新日期：2013-08-01 00:00:00