Rapid urease test for mycobacteria: preliminary observations.

abstract：:Disk diffusion tests, inoculated directly from positive blood cultures, were evaluated for accuracy of reading zone diameters after 4- and 6-h and overnight incubation. In comparisons with results from standard disk diffusion tests, the 4-h results were in agreement for 83% of tests with gram-positive organisms and 64...

journal_title：Journal of clinical microbiology

authors： Coyle MB,McGonagle LA,Plorde JJ,Clausen CR,Schoenknecht FD

更新日期：1984-09-01 00:00:00

abstract：:By the use of two reference procedures, an indirect hemagglutination assay and a complement fixation test, the presence or absence of cytomegalovirus (CMV) antibody was determined for 221 human sera. Ninety-nine sera (44.8%) were found to contain CMV antibody. The remaining 122 sera (55.2%) lacked detectable CMV antib...

journal_title：Journal of clinical microbiology

authors： Cheeseman SH,Doern GV,Ferriani RA,Keville MW,McGraw BR,Stewart JA

更新日期：1984-07-01 00:00:00

abstract：:A bacterium with a spiral shape and bipolar, single, sheathed flagella was isolated from the livers of mice with active, chronic hepatitis. The bacteria also colonized the cecal and colonic mucosae of mice. The bacterium grew at 37 degrees C under microaerophilic and anaerobic conditions, rapidly hydrolyzed urea, was ...

journal_title：Journal of clinical microbiology

authors： Fox JG,Dewhirst FE,Tully JG,Paster BJ,Yan L,Taylor NS,Collins MJ Jr,Gorelick PL,Ward JM

更新日期：1994-05-01 00:00:00

abstract：:A novel reverse primer (GLM MR1) was designed for detection of the glmM gene in Helicobacter pylori by PCR. The percentage of amplification in clinical isolates using GLM MR1 was 100% for detection of the glmM gene and 86.36% for the ureA gene. The primer designed is useful for the identification of H. pylori. ...

journal_title：Journal of clinical microbiology

authors： Espinoza MG,Vazquez RG,Mendez IM,Vargas CR,Cerezo SG

更新日期：2011-04-01 00:00:00

abstract：:Forty-eight clinical Acinetobacter isolates with different epidemic behavior were investigated for the presence of integrons and plasmids and for antibiotic susceptibility. Integrons were demonstrated in 50% of the strains by an integrase gene PCR. Epidemic strains of Acinetobacter baumannii were found to contain sign...

journal_title：Journal of clinical microbiology

authors： Koeleman JG,Stoof J,Van Der Bijl MW,Vandenbroucke-Grauls CM,Savelkoul PH

更新日期：2001-01-01 00:00:00

abstract：:A study was conducted to compare three automated systems and the Gram stain for their ability to detect significant bacteriuria. A total of 1,000 urine specimens were evaluated by Autobac MTS (General Diagnostics), Auto Microbic system (AMS; Vitek Systems, Inc.), and MS-2 (Abbott Laboratories) and compared with a semi...

journal_title：Journal of clinical microbiology

authors： Pezzlo MT,Tan GL,Peterson EM,de la Maza LM

更新日期：1982-03-01 00:00:00

abstract：:Samples from patients at high risk for invasive aspergillosis (IA) were prospectively collected and analyzed for the presence of molecular markers of fungal infection. Serum specimens were screened for galactomannan and Aspergillus DNA, and whole-blood specimens were screened only for Aspergillus DNA. Fungal infection...

journal_title：Journal of clinical microbiology

authors： Springer J,Morton CO,Perry M,Heinz WJ,Paholcsek M,Alzheimer M,Rogers TR,Barnes RA,Einsele H,Loeffler J,White PL

更新日期：2013-05-01 00:00:00

abstract：:Monoclonal antibodies were generated from mice immunized with scolex protein antigen of Cysticercus cellulosae. Three monoclonal antibodies specific for cysticercal antigens, which did not show any cross-reactivity with Taenia solium or Taenia saginata antigens, were selected. Each monoclonal antibody coupled to Sepha...

journal_title：Journal of clinical microbiology

authors： Nascimento E,Tavares CA,Lopes JD

更新日期：1987-07-01 00:00:00

abstract：:Eighty-four pneumococci with various MICs of penicillin (38 with MICs of less than or equal to 0.06 micrograms/ml [susceptible], 35 with MICs of 0.12 to 1.0 micrograms/ml [relatively resistant], and 11 with MICs of greater than 1.0 micrograms/ml [resistant] ) were screened by a disk diffusion test using oxacillin and ...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Hill BC,Thornsberry C

更新日期：1986-11-01 00:00:00

abstract：:We conducted a case-control study using the Fungitell assay, the novel Platelia Candida Antigen (Ag) Plus and Candida Antibody (Ab) Plus assays, and the Cand-Tec latex agglutination test to evaluate the usefulness of (1→3)-β-D-glucan (BDG), mannan antigen with/without anti-mannan antibody, and Cand-Tec Candida antigen...

journal_title：Journal of clinical microbiology

authors： Held J,Kohlberger I,Rappold E,Busse Grawitz A,Häcker G

更新日期：2013-04-01 00:00:00

abstract：:The pathogenic Neisseria species N. meningitidis and N. gonorrhoeae possess an outer membrane lipoprotein, designated Lip, which is present in all strains tested. The predicted protein sequence of Lip consists of a consensus AAEAP amino acid repeat. The objective of this study was to determine the feasibility of using...

journal_title：Journal of clinical microbiology

authors： Trees DL,Schultz AJ,Knapp JS

更新日期：2000-08-01 00:00:00

abstract：:Human metapneumovirus (HMPV) was the unique viral pathogen detected by a real-time reverse transcriptase PCR (RT-PCR) assay in 6 (6.4%) of 94 consecutive French children hospitalized for acute bronchiolitis from September 2001 to June 2002. This virus was identified as the third etiological cause of bronchiolitis, aft...

journal_title：Journal of clinical microbiology

authors： Bouscambert-Duchamp M,Lina B,Trompette A,Moret H,Motte J,Andréoletti L

更新日期：2005-03-01 00:00:00

abstract：:An automated enzymatic method was developed for the measurement of D-arabinitol in human serum. The assay is based on a novel, highly specific D-arabinitol dehydrogenase from Candida tropicalis. This enzyme catalyzes the oxidation of D-arabinitol to D-ribulose and the concomitant reduction of NAD+ to NADH. The NADH pr...

journal_title：Journal of clinical microbiology

authors： Switchenko AC,Miyada CG,Goodman TC,Walsh TJ,Wong B,Becker MJ,Ullman EF

更新日期：1994-01-01 00:00:00

abstract：:The ability to rapidly recognize Ebola virus infections is critical to quickly limit further spread of the disease. A rapid, sensitive, and specific laboratory diagnostic test is needed to confirm outbreaks of Ebola virus infection and to distinguish it from other diseases that can cause similar clinical symptoms. A o...

journal_title：Journal of clinical microbiology

authors： Gibb TR,Norwood DA Jr,Woollen N,Henchal EA

更新日期：2001-11-01 00:00:00

abstract：:Recent evidence supports a causal relationship between Bartonella (Rochalimaea) henselae, cat-scratch disease (CSD), and bacillary angiomatosis. Cats appear to be the primary reservoir. Blood from 19 cats owned by 14 patients diagnosed with CSD was cultured. Blood samples from cats owned by veterinary students (n = 25...

journal_title：Journal of clinical microbiology

authors： Kordick DL,Wilson KH,Sexton DJ,Hadfield TL,Berkhoff HA,Breitschwerdt EB

更新日期：1995-12-01 00:00:00

abstract：:Patients with infection from bacteremic Bartonella spp., tested using Bartonella Alphaproteobacteria growth medium (BAPGM), were retrospectively categorized into one of two groups that included those whose blood was collected once (group 1; n = 55) or three times (group 2; n = 36) within a 1-week period. Overall, 19 p...

journal_title：Journal of clinical microbiology

authors： Pultorak EL,Maggi RG,Mascarelli PE,Breitschwerdt EB

更新日期：2013-06-01 00:00:00

abstract：:The NucliSENS easyMAG automated system was compared to the column-based Qiagen method for Epstein-Barr virus (EBV) or cytomegalovirus (CMV) DNA extraction from whole blood before viral load determination using the corresponding R-gene amplification kits. Both extraction techniques exhibited a total agreement of 81.3% ...

journal_title：Journal of clinical microbiology

authors： Pillet S,Bourlet T,Pozzetto B

更新日期：2009-11-01 00:00:00

abstract：:Enterobacter cloacae strain G6809 with reduced susceptibility to carbapenems was identified from a patient in a long-term acute care hospital in Kentucky. G6809 belonged to sequence type (ST) 88 and carried two carbapenemase genes, bla(KPC-18) and bla(VIM-1). Whole-genome sequencing localized bla(KPC-18) to the chromo...

journal_title：Journal of clinical microbiology

authors： Thomson GK,Snyder JW,McElheny CL,Thomson KS,Doi Y

更新日期：2016-03-01 00:00:00

abstract：:Hepatitis E virus (HEV) genotype 3 is endemic in Europe and hyperendemic in southern France. Recent reports of a high prevalence of HEV RNA in blood donations and in culinary specialties from this geographical area confirmed the endemicity of HEV and sources of viral transmission in this geographical area. HEV causes ...

journal_title：Journal of clinical microbiology

authors： Moal V,Legris T,Motte A,Vacher-Coponat H,Fages L,Jourde-Chiche N,Borentain P,Jaubert D,Gerolami R,Colson P

更新日期：2015-05-01 00:00:00

abstract：:Rotavirus RNA was detected in the cerebrospinal fluid (CSF) of a child with central nervous system disease symptoms associated with rotavirus gastroenteritis. The rotavirus isolates from the fecal and CSF samples were genotyped as G1P[8]. Sequence analysis of the VP7 and VP4 proteins derived from the fecal and CSF sam...

journal_title：Journal of clinical microbiology

authors： Iturriza-Gómara M,Auchterlonie IA,Zaw W,Molyneaux P,Desselberger U,Gray J

更新日期：2002-12-01 00:00:00

abstract：:The IS200-like insertion sequence (IS) is a 708-bp element recently found in Yersinia pestis. Its nucleotide sequence is 85% identical to that of IS200 recovered in most Salmonella enterica isolates. It is also present in multiple copies in Y. pseudotuberculosis. In contrast, this IS is found in some (biotype 1B strai...

journal_title：Journal of clinical microbiology

authors： Odaert M,Berche P,Simonet M

更新日期：1996-09-01 00:00:00

abstract：:In Asia, bla(KPC) detection has been limited to East Asia and not yet seen in Southeast Asia. We report four bla(KPC-2)-containing Klebsiella pneumoniae isolates from two different hospitals in Singapore. All isolates belonged to strain type 11 (ST11) and were indistinguishable by pulsed-field gel electrophoresis (PFG...

journal_title：Journal of clinical microbiology

authors： Balm MN,Ngan G,Jureen R,Lin RT,Teo J

更新日期：2012-02-01 00:00:00

abstract：:The use of rapid-cycle PCR combined with colorimetric microwell hybridization for detecting Bordetella pertussis was investigated. Rapid cycling was performed with an air thermocycler (model 1605; Idaho Technology, Idaho Falls, Idaho). Although the instrument was originally designed to be used with capillary tubes, an...

journal_title：Journal of clinical microbiology

authors： Buck GE

更新日期：1996-06-01 00:00:00

abstract：:We recently developed a mass spectrometry (MS) procedure based on the detection of a serum disaccharide (MS-DS) in patients with invasive candidiasis (IC). Here, we compare the performance of MS-DS for the diagnosis of IC, invasive aspergillosis (IA), and mucormycosis (MM) with those of commercially available antigen ...

journal_title：Journal of clinical microbiology

authors： Mery A,Sendid B,François N,Cornu M,Poissy J,Guerardel Y,Poulain D

更新日期：2016-11-01 00:00:00

abstract：:Recent molecular studies have shown Mycobacterium porcinum, recovered from cases of lymphadenitis in swine, to have complete 16S rDNA sequence identity and >70% DNA-DNA homology with human isolates within the M. fortuitum third biovariant complex. We identified 67 clinical and two environmental isolates of the M. fort...

journal_title：Journal of clinical microbiology

authors： Wallace RJ Jr,Brown-Elliott BA,Wilson RW,Mann L,Hall L,Zhang Y,Jost KC Jr,Brown JM,Kabani A,Schinsky MF,Steigerwalt AG,Crist CJ,Roberts GD,Blacklock Z,Tsukamura M,Silcox V,Turenne C

更新日期：2004-12-01 00:00:00

abstract：:We developed a 4-h nested polymerase chain reaction assay that detected a region of the plasminogen activator gene of Yersinia pestis in 100% of 43 Y. pestis strains isolated from humans, rats, and fleas yet was unreactive with the closely related species Yersinia enterocolitica and Yersinia pseudotuberculosis. ...

journal_title：Journal of clinical microbiology

authors： Campbell J,Lowe J,Walz S,Ezzell J

更新日期：1993-03-01 00:00:00

abstract：:In an evaluation of the API 20NE for the identification of Burkholderia spp., 792/800 (99%) Burkholderia pseudomallei and 17/19 (89%) B. cepacia isolates were correctly identified but 10 B. mallei and 98 B. thailandensis isolates were not correctly identified. A latex agglutination test was positive for 796/800 (99.5%...

journal_title：Journal of clinical microbiology

authors： Amornchai P,Chierakul W,Wuthiekanun V,Mahakhunkijcharoen Y,Phetsouvanh R,Currie BJ,Newton PN,van Vinh Chau N,Wongratanacheewin S,Day NP,Peacock SJ

更新日期：2007-11-01 00:00:00

abstract：:Herpesviruses isolated from either domesticated or wild carnivores should be immunologically compared with known viruses of this group which could have been included in the diet of the animal before being considered to be previously undescribed herpesviruses native to the carnivore in question. ...

journal_title：Journal of clinical microbiology

authors： Porter DD,Larsen AE,Cox NA

更新日期：1975-01-01 00:00:00

abstract：:This longitudinal study evaluated the response to periodontal treatment by subjects infected with either JP2 (n = 25) or non-JP2 (n = 25) Aggregatibacter (Actinobacillus) actinomycetemcomitans. Participants were treated during the first 4 months by receiving (i) scaling and root planing, (ii) systemic antibiotic thera...

journal_title：Journal of clinical microbiology

authors： Cortelli SC,Costa FO,Kawai T,Aquino DR,Franco GC,Ohara K,Roman-Torres CV,Cortelli JR

更新日期：2009-07-01 00:00:00

abstract：:Pulsed-field gel electrophoresis revealed that multiple consecutive isolates of Legionella pneumophila from two cardiac transplant patients remained genomically stable, despite exposure to host defenses and antimicrobial agents. ...

journal_title：Journal of clinical microbiology

authors： Marrie TJ,Johnson WM,Tyler SD,Bezanson GS,Burbridge S

更新日期：1994-12-01 00:00:00