Detection of Bordetella pertussis by rapid-cycle PCR and colorimetric microwell hybridization.

abstract：:The ability of Staphylococcus aureus to clot plasma through conformational activation of prothrombin by staphylocoagulase is used to distinguish S. aureus from coagulase-negative staphylococci. We show that while the direct thrombin inhibitor dabigatran inhibits staphylocoagulase activity, the clinical use of dabigatr...

journal_title：Journal of clinical microbiology

authors： Vanassche T,Verhaegen J,Peetermans WE,Hoylaerts MF,Verhamme P

更新日期：2010-11-01 00:00:00

abstract：:Klebsiella pneumoniae carbapenemases (KPCs) are considered a serious threat to antibiotic therapy, as they confer resistance to carbapenems, which are used to treat extended-spectrum beta-lactamase (ESBL)-producing bacteria. Here, we describe the development and evaluation of a DNA microarray for the detection and gen...

journal_title：Journal of clinical microbiology

authors： Peter H,Berggrav K,Thomas P,Pfeifer Y,Witte W,Templeton K,Bachmann TT

更新日期：2012-12-01 00:00:00

abstract：:Despite attempts to control avian mycoplasmosis through management, vaccination, and surveillance, Mycoplasma gallisepticum continues to cause significant morbidity, mortality, and economic losses in poultry production. Live attenuated vaccines are commonly used in the poultry industry to control avian mycoplasmosis; ...

journal_title：Journal of clinical microbiology

authors： Ricketts C,Pickler L,Maurer J,Ayyampalayam S,García M,Ferguson-Noel NM

更新日期：2016-12-28 00:00:00

abstract：:Genotyping and subtyping of 225 samples with hepatitis C virus (HCV) genotype 1, 2, 3, or 6 infection were done with Versant LiPA 2.0 and Abbott RealTime HCV Genotype (GT) II by using direct sequencing of the NS5B and 5' untranslated regions as the reference standards. The concordance rates were >99.2% for genotypes a...

journal_title：Journal of clinical microbiology

authors： Liu CH,Liang CC,Liu CJ,Lin CL,Su TH,Yang HC,Chen PJ,Chen DS,Kao JH

更新日期：2015-05-01 00:00:00

abstract：:An immunofluorescent-antibody test was developed for rapid detection of Pseudomonas aeruginosa in blood cultures. The test uses a murine monoclonal antibody specific for all strains of P. aeruginosa. In initial tests, bright uniform immunofluorescence signals were seen when each of the 17 international serotypes, as w...

journal_title：Journal of clinical microbiology

authors： Counts GW,Schwartz RW,Ulness BK,Hamilton DJ,Rosok MJ,Cunningham MD,Tam MR,Darveau RP

更新日期：1988-06-01 00:00:00

abstract：:Comprehensive data on the prevalence of quinolone resistance in Mycobacterium tuberculosis clinical isolates in the United States are scarce. By use of a systematic population-based approach, M. tuberculosis strains from tuberculosis (TB) cases were collected in Harris County, TX, in 2007 to 2008. The susceptibilities...

journal_title：Journal of clinical microbiology

authors： El Sahly HM,Teeter LD,Jost KC Jr,Dunbar D,Lew J,Graviss EA

更新日期：2011-08-01 00:00:00

abstract：:A total of 53 strains of enterococci, including recently isolated strains with high-level resistance to vancomycin, were tested for vancomycin susceptibility by broth microdilution and disk diffusion using Mueller-Hinton media with and without supplementation with 5% blood. By using currently published parameters of t...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Hill BC,Thornsberry C

更新日期：1989-09-01 00:00:00

abstract：:The dimorphic fungus, Penicillium marneffei, is an emerging opportunistic pathogen endemic in Southeast Asia, especially for those with impaired cellular immunity such as human immunodeficiency virus-infected persons. A discriminatory and reproducible method based on the analysis of nucleotide sequences would facilita...

journal_title：Journal of clinical microbiology

authors： Lasker BA

更新日期：2006-09-01 00:00:00

abstract：:Diarrheal diseases are highly prevalent in Bangladesh. However, the relative contribution of diarrheagenic Escherichia coli organisms--those that are enterotoxigenic (ETEC), enteropathogenic (EPEC), enteroinvasive, enterohemorrhagic, enteroaggregative, and diffuse adherent--to diarrhea in Bangladeshi populations is no...

journal_title：Journal of clinical microbiology

authors： Albert MJ,Faruque SM,Faruque AS,Neogi PK,Ansaruzzaman M,Bhuiyan NA,Alam K,Akbar MS

更新日期：1995-04-01 00:00:00

abstract：:Using 920 sera, we compared the specificity and reproducibility of the hemagglutination treponemal test for syphilis with those of the fluorescent treponemal antibody-absorption test and the microhemagglutination assay for Treponema pallidum antibodies; we found all three tests to be comparable. However, the hemagglut...

journal_title：Journal of clinical microbiology

authors： Larsen SA,Hambie EA,Pettit DE,Perryman MW,Kraus SJ

更新日期：1981-10-01 00:00:00

abstract：:The specificity of Mycobacterium kansasii anti-mycoside A antiserum prepared in rabbits injected with purified samples of the phenolic glycolipid was evaluated by an enzyme-linked immunosorbent assay. Chloroform-methanol extracts from representative strains of 23 mycobacterial species and 50 strains of M. kansasii sho...

journal_title：Journal of clinical microbiology

authors： Papa F,Riviere M,Fournié JJ,Puzo G,David H

更新日期：1987-12-01 00:00:00

abstract：:The Leishmania species cause a variety of human disease syndromes. Methods for diagnosis and species differentiation are insensitive and many require invasive sampling. Although quantitative PCR (qPCR) methods are reported for leishmania detection, no systematic method to quantify parasites and determine the species i...

journal_title：Journal of clinical microbiology

authors： Weirather JL,Jeronimo SM,Gautam S,Sundar S,Kang M,Kurtz MA,Haque R,Schriefer A,Talhari S,Carvalho EM,Donelson JE,Wilson ME

更新日期：2011-11-01 00:00:00

abstract：:We sought evidence of toxoplasma parasitemia among 37 people with active or dormant Toxoplasma gondii infection or no evidence of infection. DNA was extracted from erythrocyte-free portions of blood samples, and the T. gondii B1 gene was amplified by the polymerase chain reaction. Evidence of T. gondii parasitemia was...

journal_title：Journal of clinical microbiology

authors： Filice GA,Hitt JA,Mitchell CD,Blackstad M,Sorensen SW

更新日期：1993-09-01 00:00:00

abstract：:We evaluated the Phoenix automated microbiology system (BD Diagnostic Systems, Sparks, MD) for the identification (ID) and antimicrobial susceptibility testing (AST) of challenge and clinical staphylococci and enterococci recovered from patients in a tertiary-care medical center. In total, 424 isolates were tested: 90...

journal_title：Journal of clinical microbiology

authors： Carroll KC,Borek AP,Burger C,Glanz B,Bhally H,Henciak S,Flayhart DC

更新日期：2006-06-01 00:00:00

abstract：:Monitoring antiretroviral therapy requires that human immunodeficiency virus type 1 (HIV-1) viremia assays are applicable to all distinct variants. This study evaluates the performance of three commercial viral load assays-Versant HIV-1 RNA bDNA v3.0, Cobas AmpliPrep/Cobas TaqMan HIV-1, and NucliSens HIV-1 EasyQ v1.2-...

journal_title：Journal of clinical microbiology

authors： Holguín A,López M,Molinero M,Soriano V

更新日期：2008-09-01 00:00:00

abstract：:Detection of methicillin (meticillin)-resistant Staphylococcus aureus colonization was assessed using combined nose and groin swabs in two commercial PCR assays (the Xpert MRSA assay and the BD GeneOhm MRSA assay). Compared to routine culture, both had similar sensitivities (87.0% versus 84.8%, respectively) and speci...

journal_title：Journal of clinical microbiology

authors： Kelley PG,Grabsch EA,Howden BP,Gao W,Grayson ML

更新日期：2009-11-01 00:00:00

abstract：:Sixty rotavirus-positive stool specimens from children with diarrhea were classified into G and P genotypes. G typing was done by PCR and then by hybridization with G type-specific (G1 to G4) oligonucleotide probes, whereas nested PCR was performed for P typing. Thirty-nine samples could be classified into both G and ...

journal_title：Journal of clinical microbiology

authors： Husain M,Seth P,Dar L,Broor S

更新日期：1996-06-01 00:00:00

abstract：:Prion diseases of cattle include the classical bovine spongiform encephalopathy (C-BSE) and the atypical H-type BSE (H-BSE) and L-type BSE (L-BSE) strains. Although the C- and L-BSE strains can be detected and discriminated by ultrasensitive real-time quaking-induced conversion (RT-QuIC) assays, no such test has yet b...

journal_title：Journal of clinical microbiology

authors： Masujin K,Orrú CD,Miyazawa K,Groveman BR,Raymond LD,Hughson AG,Caughey B

更新日期：2016-03-01 00:00:00

abstract：:With improved measles virus (MV) control, the genetic variability of the MV-nucleoprotein hypervariable region (NP-HVR) decreases. Thus, it becomes increasingly difficult to determine the origin of a virus using only this part of the genome. During outbreaks in Europe and Africa, we found MV strains with identical NP-...

journal_title：Journal of clinical microbiology

authors： Kessler JR,Kremer JR,Shulga SV,Tikhonova NT,Santibanez S,Mankertz A,Semeiko GV,Samoilovich EO,Tamfum JJ,Pukuta E,Muller CP

更新日期：2011-02-01 00:00:00

abstract：:The Clinical and Laboratory Standards Institute (CLSI) has revised several breakpoints since 2010 for bacteria that grow aerobically. In 2019, these revisions include changes to the ciprofloxacin and levofloxacin breakpoints for the Enterobacteriaceae and Pseudomonas aeruginosa, daptomycin breakpoints for Enterococcus...

journal_title：Journal of clinical microbiology

authors： Humphries RM,Abbott AN,Hindler JA

更新日期：2019-05-24 00:00:00

abstract：:We assessed the genetic and the antigenic variability within the env gene of peripheral blood human immunodeficiency virus (HIV) type 1 (HIV-1) group O populations during the natural course of a female heterosexual infection. Our data revealed the existence of a significant increase in amino acid sequence variability ...

journal_title：Journal of clinical microbiology

authors： Andréoletti L,Réveil B,Moret H,Brodard V,Philbert F,Tabary T,Cohen JH

更新日期：2007-04-01 00:00:00

abstract：:Whether targeted or universal decolonization strategies for the control of methicillin-resistant Staphylococcus aureus (MRSA) select for resistance to decolonizing agents is unresolved. The REDUCE-MRSA trial (ClinicalTrials registration no. NCT00980980) provided an opportunity to investigate this question. REDUCE-MRSA...

journal_title：Journal of clinical microbiology

authors： Hayden MK,Lolans K,Haffenreffer K,Avery TR,Kleinman K,Li H,Kaganov RE,Lankiewicz J,Moody J,Septimus E,Weinstein RA,Hickok J,Jernigan J,Perlin JB,Platt R,Huang SS

更新日期：2016-11-01 00:00:00

abstract：:Fast and accurate identification and typing of pathogens are essential for effective surveillance and outbreak detection. The current routine procedure is based on a variety of techniques, making the procedure laborious, time-consuming, and expensive. With whole-genome sequencing (WGS) becoming cheaper, it has huge po...

journal_title：Journal of clinical microbiology

authors： Joensen KG,Scheutz F,Lund O,Hasman H,Kaas RS,Nielsen EM,Aarestrup FM

更新日期：2014-05-01 00:00:00

abstract：:A novel lateral flow immunochromatographic device (LFD) was evaluated in several veterinary diagnostic laboratories. It was confirmed to be specific for Mycobacterium bovis and M.caprae cells. The performance of the novel LFD was assessed relative to the confirmatory tests routinely applied after culture (spoligotypin...

journal_title：Journal of clinical microbiology

authors： Stewart LD,McCallan L,McNair J,McGoldrick A,Morris R,Moyen JL,De Juan Ferré L,Romero B,Alonso E,Parsons SDC,Van Helden P,Araújo FR,Grant IR

更新日期：2017-12-01 00:00:00

abstract：:Chronic periodontitis is a common infectious disease in the adult population. The etiology is clearly bacterial, and a small number of bacterial species have been consistently associated with periodontitis, including Bacteroides forsythus and Porphyromonas gingivalis. Comparatively little attention has been paid to th...

journal_title：Journal of clinical microbiology

authors： Leys EJ,Lyons SR,Moeschberger ML,Rumpf RW,Griffen AL

更新日期：2002-03-01 00:00:00

abstract：:A typing enzyme immunoassay (TYPE-EIA) was used to determine the antigenic types of 64 astrovirus-positive specimens from nine collections from seven countries. Six of the seven known astrovirus types were detected in the collections, with HAstV-1 predominating in all collections for one from the United Kingdom. Selec...

journal_title：Journal of clinical microbiology

authors： Noel JS,Lee TW,Kurtz JB,Glass RI,Monroe SS

更新日期：1995-04-01 00:00:00

abstract：:Early detection of Mycobacterium tuberculosis complex (MTBC) and markers conveying drug resistance can have a beneficial impact on preventive public health actions. We describe here a new molecular point-of-care (POC) system, the Genedrive, which is based on simple sample preparation combined with PCR to detect MTBC a...

journal_title：Journal of clinical microbiology

authors： Castan P,de Pablo A,Fernández-Romero N,Rubio JM,Cobb BD,Mingorance J,Toro C

更新日期：2014-02-01 00:00:00

abstract：:Recurrence of osteomyelitis by the same bacterial strain is well known. We report three patients with a second episode of osteomyelitis at the same site caused by different strains of bacteria from the original. Formerly infected and altered bone surface might present a region of diminished resistance for a new infect...

journal_title：Journal of clinical microbiology

authors： Uçkay I,Assal M,Legout L,Rohner P,Stern R,Lew D,Hoffmeyer P,Bernard L

更新日期：2006-03-01 00:00:00

abstract：:Two clones, CA4-2 and CA5-4, which produced agglutinating monoclonal immunoglobulin M (IgM) antibodies (MAbs) against mannan antigens of Candida albicans serotype A, were established. The specificity of each MAb was determined by slide agglutination tests for cross-reactivity patterns against the homologous and six ot...

journal_title：Journal of clinical microbiology

authors： Miyakawa Y,Kagaya K,Fukazawa Y,Soe G

更新日期：1986-05-01 00:00:00

abstract：:Initially, multilocus enzyme electrophoresis was used to examine genetic relationships among 63 isolates of Mycobacterium bovis and 13 other members of the M. tuberculosis complex. The isolates were divided into five electrophoretic types, with a mean genetic diversity of 0.1. The strains were genetically homogenous, ...

journal_title：Journal of clinical microbiology

authors： Feizabadi MM,Robertson ID,Cousins DV,Hampson DJ

更新日期：1996-05-01 00:00:00