Comparison of Abbott RealTime HCV Genotype II with Versant line probe assay 2.0 for hepatitis C virus genotyping.

abstract：:The ability of fluorescence resonance energy transfer, molecular-beacon, and TaqMan probes to detect single nucleotide polymorphism (SNP) in the presence of a wild-type allele was evaluated using drug resistance-conferring SNPs in mixed Mycobacterium tuberculosis DNA. It was found that both the absolute quantity and t...

journal_title：Journal of clinical microbiology

authors： Yesilkaya H,Meacci F,Niemann S,Hillemann D,Rüsch-Gerdes S,LONG DRUG Study Group.,Barer MR,Andrew PW,Oggioni MR

更新日期：2006-10-01 00:00:00

abstract：:We used genotypic and phenotypic assays to estimate the frequency of X4/DM viruses in 131 patients infected with non-subtype-B viruses at the time of primary HIV-1 infection (PHI). All patients were enrolled in the French PRIMO Cohort from 1996 to 2007. Most strains belonged to CRF02_AG (51.1%) and subtype A (14.5%). ...

journal_title：Journal of clinical microbiology

authors： Frange P,Chaix ML,Raymond S,Galimand J,Deveau C,Meyer L,Goujard C,Rouzioux C,Izopet J,French ANRS CO 06 PRIMO Cohort Study Group.

更新日期：2010-10-01 00:00:00

abstract：:A screening assay for phospholipase C using a chromogenic substrate incorporated into agar medium is described. The assay directly visualizes phospholipase C activity of mycoplasma lysates and membranes on agar plates, or the activity may be measured by spectrophotometry. The results from the assay confirm the presenc...

journal_title：Journal of clinical microbiology

authors： De Silva NS,Quinn PA

更新日期：1987-04-01 00:00:00

abstract：:Hepatitis C virus (HCV) infection typically leads to antibody response within weeks after primary infection. Here, we describe the case of a child infected with HCV by mother-to-child transmission who remained persistently seronegative despite the presence of high levels of circulating HCV RNA. ...

journal_title：Journal of clinical microbiology

authors： Larouche A,Gaëtan G,El-Bilali N,Quesnel-Vallières M,Martin SR,Alvarez F,Shoukry NH,Soudeyns H

更新日期：2012-07-01 00:00:00

abstract：:A total of 4,409 stool specimens from infants less than 5 years of age seeking treatment for diarrhea in Matlab, Bangladesh, were tested for the presence of adenoviruses by using an enzyme immunoassay (EIA). EIA-positive stool samples were serotyped with monoclonal antibodies specific for adenovirus type 40 (Ad40) and...

journal_title：Journal of clinical microbiology

authors： Jarecki-Khan K,Tzipori SR,Unicomb LE

更新日期：1993-03-01 00:00:00

abstract：:The present study describes a method for amplifying DNA in Actinobacillus actinomycetemcomitans by using short, synthetic oligonucleotides of random sequence as primers in the polymerase chain reaction. Genomic DNA from each of 20 human isolates of A. actinomycetemcomitans was successfully amplified in a thermal cycle...

journal_title：Journal of clinical microbiology

authors： Preus HR,Haraszthy VI,Zambon JJ,Genco RJ

更新日期：1993-10-01 00:00:00

abstract：:Seventy-six nontuberculous mycobacterial isolates obtained from patients living in Greece were analyzed with the GenoType Mycobacterium CM (for common mycobacteria) and AS (for additional species) assays. GenoType correctly identified all but one of the mycobacterial species. For this species, additional probes should...

journal_title：Journal of clinical microbiology

authors： Gitti Z,Neonakis I,Fanti G,Kontos F,Maraki S,Tselentis Y

更新日期：2006-06-01 00:00:00

abstract：:Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human corona...

journal_title：Journal of clinical microbiology

authors： Chan JF,Choi GK,Tsang AK,Tee KM,Lam HY,Yip CC,To KK,Cheng VC,Yeung ML,Lau SK,Woo PC,Chan KH,Tang BS,Yuen KY

更新日期：2015-08-01 00:00:00

abstract：:We evaluated the performance characteristics of a new, real-time nucleic acid sequence-based amplification (NASBA) assay that incorporates molecular beacon technology for detection of human immunodeficiency virus type 1 (HIV-1). The quantitative results were comparable to those obtained with three leading commercially...

journal_title：Journal of clinical microbiology

authors： McClernon DR,Vavro C,St Clair M

更新日期：2006-06-01 00:00:00

abstract：:Bovine vaginal cytobrush specimens were analyzed for the presence of Chlamydia spp. by a high-sensitivity, high-specificity quantitative PCR. The 53% prevalence of low-level Chlamydia psittaci and C. pecorum genital infection detected in virgin heifers suggests predominantely extragenital transmission of Chlamydia in ...

journal_title：Journal of clinical microbiology

authors： DeGraves FJ,Gao D,Hehnen HR,Schlapp T,Kaltenboeck B

更新日期：2003-04-01 00:00:00

abstract：:Traditional ribotyping detects genomic restriction fragment length polymorphisms by probing chromosomal DNA with rRNA. Although it is a powerful method for determining the molecular epidemiology of bacterial pathogens, technical difficulties limit its application. As an alternative, polymorphisms were sought in the 16...

journal_title：Journal of clinical microbiology

authors： Kostman JR,Edlind TD,LiPuma JJ,Stull TL

更新日期：1992-08-01 00:00:00

abstract：:A total of 151 Escherichia coli strains resistant to cefotaxime and ceftazidime were isolated during a prospective surveillance study. These strains were characterized by clinical, microbiological, and molecular analyses and were distributed into four clusters of 103, 11, 6, and 5 isolates, along with 25 unrelated str...

journal_title：Journal of clinical microbiology

authors： Oteo J,Navarro C,Cercenado E,Delgado-Iribarren A,Wilhelmi I,Orden B,García C,Miguelañez S,Pérez-Vázquez M,García-Cobos S,Aracil B,Bautista V,Campos J

更新日期：2006-07-01 00:00:00

abstract：:With improved measles virus (MV) control, the genetic variability of the MV-nucleoprotein hypervariable region (NP-HVR) decreases. Thus, it becomes increasingly difficult to determine the origin of a virus using only this part of the genome. During outbreaks in Europe and Africa, we found MV strains with identical NP-...

journal_title：Journal of clinical microbiology

authors： Kessler JR,Kremer JR,Shulga SV,Tikhonova NT,Santibanez S,Mankertz A,Semeiko GV,Samoilovich EO,Tamfum JJ,Pukuta E,Muller CP

更新日期：2011-02-01 00:00:00

abstract：:The incidence of vancomycin resistance among enterococci is increasing in the United States and elsewhere in the world, but automated susceptibility testing methods have difficulty detecting resistance expressed by certain strains. The agar screening method described by Willey et al. (B. M. Willey, B. N. Kreiswirth, A...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Clark NC,Ferraro MJ,Sahm DF,Doern G,Pfaller MA,Reller LB,Weinstein MP,Zabransky RJ,Tenover FC

更新日期：1994-07-01 00:00:00

abstract：:Serum hepatitis B virus (HBV) DNA was extracted from a chronically infected patient with cocirculation of hepatitis B surface antigen (HBsAg) and anti-HBs antibodies. Direct PCR and clone-derived sequences of the S and overlapped P genes were obtained. DNA sequences and phylogenetic analysis ascribed this isolate to g...

journal_title：Journal of clinical microbiology

authors： Cuestas ML,Mathet VL,Ruiz V,Minassian ML,Rivero C,Sala A,Corach D,Alessio A,Pozzati M,Frider B,Oubiña JR

更新日期：2006-06-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of immunoglobulin G (IgG) to the capsular polysaccharide (CP) of Pasteurella haemolytica serotype 1. Purified CP was first covalently coupled to poly-L-lysine and then optimally adsorbed at a concentration of 5 micrograms/ml to microtiter pla...

journal_title：Journal of clinical microbiology

authors： Townsend EL,Maheswaran SK,Leininger JR,Ames TR

更新日期：1987-02-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) strains display genetic polymorphisms, and these polymorphisms can be analyzed to study viral transmission and pathogenesis. Recently, short tandem repeat (STR) length polymorphisms have been identified in the HCMV genome. We assessed the utility of STRs in characterizing HCMV strains and ...

journal_title：Journal of clinical microbiology

authors： Walker A,Petheram SJ,Ballard L,Murph JR,Demmler GJ,Bale JF Jr

更新日期：2001-06-01 00:00:00

abstract：:This study examined the genetic diversity and dynamicity of circulating Mycobacterium tuberculosis strains in Thailand using nearly neutral molecular markers. The single nucleotide polymorphism (SNP)-based genotypes of 1,414 culture-positive M. tuberculosis isolates from 1,282 pulmonary tuberculosis (PTB) and 132 extr...

journal_title：Journal of clinical microbiology

authors： Srilohasin P,Chaiprasert A,Tokunaga K,Nishida N,Prammananan T,Smittipat N,Mahasirimongkol S,Chaiyasirinroje B,Yanai H,Palittapongarnpim P

更新日期：2014-12-01 00:00:00

abstract：:We report a case of pneumonia with a fatal outcome caused by a nonhemolytic strain of Streptococcus pyogenes. This strain was isolated in pure growth from blood cultures and was fully identified biochemically. Such strains will be difficult to recognize and isolate from sites with heavy growth of normal flora. This ph...

journal_title：Journal of clinical microbiology

authors： Taylor MB,Barkham T

更新日期：2002-06-01 00:00:00

abstract：:Automated sequence analysis of a 410-bp region of the axyR gene in 105 Mycobacterium tuberculosis complex isolates identified a polymorphic nucleotide that differentiated Mycobacterium bovis isolates from other complex members. All 29 M. bovis isolates sequenced had an adenine residue at nucleotide 285, whereas all 76...

journal_title：Journal of clinical microbiology

authors： Sreevatsan S,Escalante P,Pan X,Gillies DA 2nd,Siddiqui S,Khalaf CN,Kreiswirth BN,Bifani P,Adams LG,Ficht T,Perumaalla VS,Cave MD,van Embden JD,Musser JM

更新日期：1996-08-01 00:00:00

abstract：:VP1 sequences were determined for poliovirus type 1 isolates obtained over a 189-day period from a poliomyelitis patient with common variable immunodeficiency syndrome (a defect in antibody formation). The isolate from the first sample, taken 11 days after onset of paralysis, contained two poliovirus populations, diff...

journal_title：Journal of clinical microbiology

authors： Kew OM,Sutter RW,Nottay BK,McDonough MJ,Prevots DR,Quick L,Pallansch MA

更新日期：1998-10-01 00:00:00

abstract：:A total of 63 clinical isolates of Proteus mirabilis collected over a 19-month period were typed by the Dienes test and ribotyping. Ribotyping was performed using the fully automated RiboPrinter Microbial Characterization System (Qualicon, Wilmington, Del.). Isolates that were indistinguishable by the Dienes test and/...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Mujeeb I,Hollis RJ,Jones RN,Doern GV

更新日期：2000-03-01 00:00:00

abstract：:In recent studies, antigenic divergence has been observed in Bordetella pertussis circulating isolates. We collected 80 Bordetella pertussis isolates in Taiwan from 1998 to 2004 and analyzed them using a combination of pulsed-field gel electrophoresis (PFGE) and sequencing of the ptxS1 and prn genes. The incidence of ...

journal_title：Journal of clinical microbiology

authors： Yao SM,Lin YC,Chou CY,Chen YY,Hsiao MJ,Chen HY,Yan JJ,Su HP,Li SY

更新日期：2005-11-01 00:00:00

abstract：:The OmpK36 porin plays a role in carbapenem resistance and may contribute to bacterial virulence in Klebsiella pneumoniae. This study aimed to investigate the characteristics of different groups of K. pneumoniae separated by ompK36 typing. Among 226 nonduplicate K. pneumoniae bloodstream isolates collected at a Taiwan...

journal_title：Journal of clinical microbiology

authors： Yan JJ,Zheng PX,Wang MC,Tsai SH,Wang LR,Wu JJ

更新日期：2015-10-01 00:00:00

abstract：:The spores of Henneguya salminicola, a common tissue parasite of salmonid fishes in the northern hemisphere, were observed in stool specimens from two different patients with diarrhea. The spores' superficial resemblance to human spermatozoa resulted, in one instance, in an incorrect report, leading to suspicion of se...

journal_title：Journal of clinical microbiology

authors： McClelland RS,Murphy DM,Cone DK

更新日期：1997-11-01 00:00:00

abstract：:Seventy-eight blood cultures with a Gram stain result of Gram-positive cocci in pairs and/or chains were evaluated with the Nanosphere Verigene Gram-positive blood culture (BC-GP) assay. The overall concordance of the assay with culture was 89.7% (70/78 cultures), allowing for the development of a targeted treatment a...

journal_title：Journal of clinical microbiology

authors： Alby K,Daniels LM,Weber DJ,Miller MB

更新日期：2013-11-01 00:00:00

abstract：:A severe outbreak of hemorrhagic colitis occurred in London, Ontario, during the month of September 1985. A total of 55 residents and 18 employees of a nursing home developed diarrhea, and 17 residents (age range, 78 to 99 years) died. Specimens from 38 patients, 37 employees and contacts, and 10 autopsies were invest...

journal_title：Journal of clinical microbiology

authors： Krishnan C,Fitzgerald VA,Dakin SJ,Behme RJ

更新日期：1987-06-01 00:00:00

abstract：:Influenza epidemics arise through the accumulation of viral genetic changes, culminating in a novel antigenic type that is able to escape host immunity. Following an outbreak of the A/Fujian/411/2002-like strains in Asia, including China, Japan, and South Korea, in 2002, Australia and New Zealand experienced substanti...

journal_title：Journal of clinical microbiology

authors： Kang S,Yang IS,Lee JY,Park Y,Oh HB,Kang C,Kim KH

更新日期：2010-06-01 00:00:00

abstract：:We have developed and validated a nested real-time PCR (NRT-PCR) for the genotyping of Chlamydia trachomatis and used it specifically for the typing of either eight genovars from D to K or three genovars of lymphogranuloma venereum (LGV). The 11 probes used in the NRT-PCR correctly identified the DNA from D to K and L...

journal_title：Journal of clinical microbiology

authors： Jalal H,Stephen H,Alexander S,Carne C,Sonnex C

更新日期：2007-08-01 00:00:00

abstract：:The objective of this hospital-based study was to determine the relationship between colonizing and infecting strains of Candida species and Torulopsis glabrata. Surveillance cultures from high-risk patients were paired with subsequent bloodstream isolates. Organisms were typed by using restriction endonuclease digest...

journal_title：Journal of clinical microbiology

authors： Reagan DR,Pfaller MA,Hollis RJ,Wenzel RP

更新日期：1990-12-01 00:00:00