Identification and typing of Malassezia species by amplified fragment length polymorphism and sequence analyses of the internal transcribed spacer and large-subunit regions of ribosomal DNA.

abstract：:Maternal prenatal screening for group B streptococci (GBS) followed by offering of intrapartum chemoprophylaxis to carriers is one of the strategies used to reduce the incidence of neonatal early-onset GBS infections. Culturing of vaginal and anorectal swab specimens in selective broth is the screening procedure recom...

journal_title：Journal of clinical microbiology

authors： Hansen SM,Sørensen UB

更新日期：2003-04-01 00:00:00

abstract：:Acute and convalescent-phase sera obtained from 10 patients infected with a Japanese strain of spotted fever group (SFG) rickettsia were tested by the indirect immunoperoxidase test, the Weil-Felix test, an enzyme-linked immunosorbent assay (ELISA), and immunoblotting. By the Weil-Felix test, the reactivity of these s...

journal_title：Journal of clinical microbiology

authors： Amano K,Hatakeyama H,Okuta M,Suto T,Mahara F

更新日期：1992-09-01 00:00:00

abstract：:We found 31 different emm-toxin genotypes among 74 group A streptococcal isolates causing invasive infections in French children. The predominant emm types were emm1 (25%), emm3 (8%), emm4 (8%), emm6 (7%), and emm89 (9%). Sixteen percent of isolates harbored the streptococcal invasive locus, half of them belonging to ...

journal_title：Journal of clinical microbiology

authors： Bidet P,Courroux C,Salgueiro C,Carol A,Mariani-Kurkdjian P,Bonacorsi S,Bingen E

更新日期：2007-06-01 00:00:00

abstract：:The detection of the human RNA viruses, calicivirus and astrovirus, requires high sensitivity and broad reactivity. A novel single-tube nested reverse transcription-PCR (RT-PCR) method is described here, in which all of the required reagents are included in the one tube; however, those required for the nested amplific...

journal_title：Journal of clinical microbiology

authors： Ratcliff RM,Doherty JC,Higgins GD

更新日期：2002-11-01 00:00:00

abstract：:Forty-two difficult-to-cultivate group A coxsackieviruses (i.e., group A types other than A7, A9, and A16), collected primarily from throat swab specimens of patients suffering from fever, pharyngitis, lymphadenopathy, and cough during the 1986 enterovirus season, were isolated in less than 24-h-old suckling mice. Thi...

journal_title：Journal of clinical microbiology

authors： Lipson SM,Walderman R,Costello P,Szabo K

更新日期：1988-07-01 00:00:00

abstract：:Coronavirus disease (COVID) serological tests are essential to determine the overall seroprevalence of a population and to facilitate exposure estimates within that population. We performed a head-to-head assessment of enzyme immunoassays (EIAs) and point-of-care lateral flow assays (POCTs) to detect severe acute resp...

journal_title：Journal of clinical microbiology

authors： Charlton CL,Kanji JN,Johal K,Bailey A,Plitt SS,MacDonald C,Kunst A,Buss E,Burnes LE,Fonseca K,Berenger BM,Schnabl K,Hu J,Stokes W,Zelyas N,Tipples G

更新日期：2020-09-22 00:00:00

abstract：:Actinobacillus pleuropneumoniae strains of serotypes 1 and 9 were studied for their serological properties by means of agglutination, coagglutination (CoA), indirect hemagglutination (IHA), Co-IHA, ring precipitation (RP), and immunodiffusion (ID) tests. Particulate and soluble antigens of unheated and heat-treated ba...

journal_title：Journal of clinical microbiology

authors： Mittal KR

更新日期：1990-03-01 00:00:00

abstract：:In a multicenter study, 113 blood samples from 19 organ transplant patients were analyzed for cytomegalovirus by the pp65 antigenemia assay and a quantitative DNA hybridization assay. Overall, there was 84% agreement among the results obtained by the two tests. Fifteen of 16 episodes of active infection were detected ...

journal_title：Journal of clinical microbiology

authors： Bossart W,Bienz K,Wunderli W

更新日期：1997-12-01 00:00:00

abstract：:Lipovitellin-salt-mannitol (LSM) plate medium was examined for its ability to directly isolate, recover, and presumptively identify Staphylococcus aureus from 418 clinical specimens. The criteria for medium evaluation included colony morphology reactions, selectivity, and ease of isolation. For 298 specimens used for ...

journal_title：Journal of clinical microbiology

authors： Merlino J,Gill R,Robertson GJ

更新日期：1996-12-01 00:00:00

abstract：:Buruli ulcer, an infection caused by Mycobacterium ulcerans, is, after tuberculosis and leprosy, the third most common mycobacterial disease. The mode of transmission of M. ulcerans is not exactly known, but since Buruli ulcer often occurs in focalized swampy areas, it is assumed that there is a reservoir of the patho...

journal_title：Journal of clinical microbiology

authors： Rondini S,Mensah-Quainoo E,Troll H,Bodmer T,Pluschke G

更新日期：2003-09-01 00:00:00

abstract：:The recent Zaire Ebola virus (EBOV) outbreak in West Africa illustrates clearly the need for additional studies with humans and animals to elucidate the ecology of Ebola viruses (EBVs). In this study, we developed a serological assay based on the Luminex technology. Nine recombinant proteins representing different vir...

journal_title：Journal of clinical microbiology

authors： Ayouba A,Touré A,Butel C,Keita AK,Binetruy F,Sow MS,Foulongne V,Delaporte E,Peeters M

更新日期：2016-12-28 00:00:00

abstract：:Shortly after adopting a 6-week-old cat, a veterinarian was bitten on the left index finger. Within 3 weeks, he developed headache, fever, and left axillary lymphadenopathy. Initial blood cultures from the cat and veterinarian were sterile. Repeat cultures from the cat grew Bartonella-like organisms with lophotrichous...

journal_title：Journal of clinical microbiology

authors： Kordick DL,Hilyard EJ,Hadfield TL,Wilson KH,Steigerwalt AG,Brenner DJ,Breitschwerdt EB

更新日期：1997-07-01 00:00:00

abstract：:A premarket trial of the Becton Dickinson Directigen respiratory syncytial virus membrane-based enzyme immunoassay compared the test with virus isolation for the detection of respiratory syncytial virus in 583 nasopharyngeal aspirates. After modification, the Directigen test showed a sensitivity of 83% and a specifici...

journal_title：Journal of clinical microbiology

authors： Kok T,Barancek K,Burrell CJ

更新日期：1990-06-01 00:00:00

abstract：:From January 2011 to December 2013, a total of 262 samples, from 188 patients suspected of having syphilis were tested for the presence of treponemal DNA by PCR amplification of five chromosomal loci, including the polA (TP0105), tmpC (TP0319), TP0136, TP0548, and 23S rRNA genes. Altogether, 146 samples from 103 patie...

journal_title：Journal of clinical microbiology

authors： Grillová L,Pĕtrošová H,Mikalová L,Strnadel R,Dastychová E,Kuklová I,Kojanová M,Kreidlová M,Vaňousová D,Hercogová J,Procházka P,Zákoucká H,Krchňáková A,Vašků V,Šmajs D

更新日期：2014-10-01 00:00:00

abstract：:A methodologic study was performed to compare the polymerase chain reaction (PCR) and Southern blot hybridization, two commonly used testing strategies for the detection of human papillomavirus (HPV) infection. Three laboratories tested masked aliquots of exfoliated cervical cell specimens obtained from 120 women by c...

journal_title：Journal of clinical microbiology

authors： Schiffman MH,Bauer HM,Lorincz AT,Manos MM,Byrne JC,Glass AG,Cadell DM,Howley PM

更新日期：1991-03-01 00:00:00

abstract：:Parasite-specific humoral and cellular immune responses were evaluated in in nine immunosuppressed patients with Strongyloides stercoralis infection. Four patients had the disseminated form of the disease, and five had serious manifestations without evidence of hyperinfection. All patients had high levels of immunoglo...

journal_title：Journal of clinical microbiology

authors： Genta RM,Douce RW,Walzer PD

更新日期：1986-06-01 00:00:00

abstract：:The CO2, O2, and pH levels of commercially available blood culture bottles with tryptic soy broth medium from five different manufacturers were compared. Ranges of 1.3 to 6.9% for CO2, 1.1 to 6.0% for O2, and pH 6.94 to 7.26 were found. Different venting procedures revealed that blood culture bottles from which the ru...

journal_title：Journal of clinical microbiology

authors： Welch WD,Porschen RK,Zarifi SZ

更新日期：1984-11-01 00:00:00

abstract：:A newly developed latex agglutination assay for the detection of genus-specific Leptospira antibodies in human sera was evaluated. The assay is performed by mixing, on an agglutination card, serum with equal volumes of stabilized antigen-coated, dyed test and control latex beads and is read within 2 min. The latex agg...

journal_title：Journal of clinical microbiology

authors： Smits HL,van der Hoorn MA,Goris MG,Gussenhoven GC,Yersin C,Sasaki DM,Terpstra WJ,Hartskeerl RA

更新日期：2000-03-01 00:00:00

abstract：:Many central vascular catheters (CVCs) are removed unnecessarily because current diagnostic methods for CVC-associated infection are unreliable. A quantitative PCR assay using primers and probe targeted to bacterial 16S ribosomal DNA was used to measure the levels of bacterial DNA in blood samples drawn through the CV...

journal_title：Journal of clinical microbiology

authors： Warwick S,Wilks M,Hennessy E,Powell-Tuck J,Small M,Sharp J,Millar MR

更新日期：2004-04-01 00:00:00

abstract：:A new test principle for the simultaneous detection of total approximate titers and immunoglobulin M antibodies has been developed and applied to the detection of antibody to hepatitis B core antigen. The method is based on the combination of a competition radioimmunoassay, for the determination of total antibody tite...

journal_title：Journal of clinical microbiology

authors： Angarano G,Monno L,Santantonio TA,Pastore G

更新日期：1984-06-01 00:00:00

abstract：:Three forms of DNA-based typing procedures for Aspergillus fumigatus isolates have been developed over the last five years. The procedures are random amplification of polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) detection, and Southern hybridizations with various repetitive sequence-based pr...

journal_title：Journal of clinical microbiology

authors： Anderson MJ,Gull K,Denning DW

更新日期：1996-01-01 00:00:00

abstract：:Blood culture bottles containing antibiotic binding resins are routinely used to minimize artificial sterilization in the presence of antibiotics. However, the resin binding kinetics can differ between antibiotics and concentrations. This study assessed the impact of clinically meaningful peak, midpoint, and trough co...

journal_title：Journal of clinical microbiology

authors： Chen IH,Nicolau DP,Kuti JL

更新日期：2019-09-24 00:00:00

abstract：:The role of atypical enteropathogenic Escherichia coli (EPEC) in childhood diarrhea is controversial. The aim of the present study was to search for genes linked with diarrhea in atypical EPEC strains from a case-control study among Norwegian children. Using DNA microarray analysis, genomic DNAs from strains isolated ...

journal_title：Journal of clinical microbiology

authors： Afset JE,Bruant G,Brousseau R,Harel J,Anderssen E,Bevanger L,Bergh K

更新日期：2006-10-01 00:00:00

abstract：:Nineteen strains of nutritionally variant streptococci (NVS) were tested for bacteriolytic activity and for their production of and sensitivity to streptococcal bacteriocinlike inhibitory substances (BLIS). None appeared to produce BLIS. An inverse relationship was found between the sensitivity to BLIS and the product...

journal_title：Journal of clinical microbiology

authors： Tagg JR,van de Rijn I

更新日期：1991-04-01 00:00:00

abstract：:The control of vancomycin-resistant enterococci (VRE) has become an increasing burden on health care resources since their discovery over 20 years ago. Current techniques employed for their detection include time-consuming and laborious phenotypic methods or molecular methods requiring costly equipment and consumables...

journal_title：Journal of clinical microbiology

authors： Griffin PM,Price GR,Schooneveldt JM,Schlebusch S,Tilse MH,Urbanski T,Hamilton B,Venter D

更新日期：2012-09-01 00:00:00

abstract：:We compared the performance of a prototype version of the Hybrid Capture 3 (HC3) human papillomavirus (HPV) DNA assay to the current generation Hybrid Capture 2 (HC2) assay, both of which target 13 oncogenic HPV types, for the detection of cervical intraepithelial neoplasia grade 3 and cancer (CIN3+) with cervicovagin...

journal_title：Journal of clinical microbiology

authors： Castle PE,Lorincz AT,Scott DR,Sherman ME,Glass AG,Rush BB,Wacholder S,Burk RD,Manos MM,Schussler JE,Macomber P,Schiffman M

更新日期：2003-09-01 00:00:00

abstract：:A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregati...

journal_title：Journal of clinical microbiology

authors： Toma C,Lu Y,Higa N,Nakasone N,Chinen I,Baschkier A,Rivas M,Iwanaga M

更新日期：2003-06-01 00:00:00

abstract：:Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one comme...

journal_title：Journal of clinical microbiology

authors： Ye S,Lambert SB,Grimwood K,Roczo-Farkas S,Nimmo GR,Sloots TP,Kirkwood CD,Whiley DM

更新日期：2015-01-01 00:00:00

abstract：:We used a nested PCR with Borrelia flagellin gene (flaB) primers and DNA sequencing to determine if Borrelia lonestari was present in Amblyomma americanum ticks removed from military personnel and sent to the Tick-Borne Disease Laboratory of the U.S. Army Center for Health Promotion and Preventive Medicine. In our pre...

journal_title：Journal of clinical microbiology

authors： Stromdahl EY,Williamson PC,Kollars TM Jr,Evans SR,Barry RK,Vince MA,Dobbs NA

更新日期：2003-12-01 00:00:00

abstract：:The use of rapid-cycle PCR combined with colorimetric microwell hybridization for detecting Bordetella pertussis was investigated. Rapid cycling was performed with an air thermocycler (model 1605; Idaho Technology, Idaho Falls, Idaho). Although the instrument was originally designed to be used with capillary tubes, an...

journal_title：Journal of clinical microbiology

authors： Buck GE

更新日期：1996-06-01 00:00:00