Abstract：
:Helicobacter cetorum sp. nov. has been cultured from the stomach of Atlantic white-sided dolphins (Lagenorhynchus acutus) and the feces of Pacific white-sided (L. obliquidens) and Atlantic bottlenose (Tursiops truncatus) dolphins and a beluga whale (Delphinapterus leucas). H. cetorum has high homology to Helicobacter pylori as shown by 16S rRNA sequencing, and H. cetorum infection has been associated with gastritis and clinical signs in cetaceans. Because the prevalence of H. cetorum in wild populations is unknown, minimally invasive techniques for detecting H. cetorum were compared for 20 wild bottlenose dolphins sampled as part of a long-term health study. Fecal samples were tested for helicobacter by culture, Southern blotting, and PCR using genus-specific and H. cetorum-specific primers. An enzyme-linked immunosorbent assay (ELISA) was developed to measure H. cetorum immunoglobulin G (IgG). H. cetorum was cultured from 4 of 20 fecal samples, 7 samples were positive using Helicobacter sp. PCR, and 8 samples were positive for H. cetorum using species-specific primers. Two additional fecal samples were positive by Helicobacter sp. Southern blotting, suggesting infection with another helicobacter. All 20 sera contained high levels of IgG antibodies to H. cetorum that were significantly lowered by preabsorption of the sera with whole-cell suspensions of H. cetorum (P < 0.02). Until the specificity of the serum ELISA can be determined by testing sera from dolphins confirmed to be uninfected, PCR and Southern blot screenings of feces are the most sensitive techniques for detection of H. cetorum, and results indicate there is at least a 50% prevalence of H. cetorum infection in these dolphins.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Harper CG,Whary MT,Feng Y,Rhinehart HL,Wells RS,Xu S,Taylor NS,Fox JGdoi
10.1128/jcm.41.7.2842-2848.2003subject
Has Abstractpub_date
2003-07-01 00:00:00pages
2842-8issue
7eissn
0095-1137issn
1098-660Xjournal_volume
41pub_type
杂志文章abstract：:Twenty (1.4%) of 1,421 adult Ixodes pacificus ticks and 2 (20%) of 10 adult Ixodes neotomae ticks collected in five counties of northern California were found to contain spirochetes by direct immunofluorescence examination of their tissues with a polyvalent conjugate. Borreliae isolated from the tissues of nine of the...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.27.10.2344-2349.1989
更新日期：1989-10-01 00:00:00
abstract：:We have developed a hexaplex PCR assay for rapid detection of the virulence and regulatory genes for cholera toxin enzymatic subunit A (ctxA), zonula occludens toxin (zot), accessory cholera enterotoxin (ace), toxin-coregulated pilus (tcpA), outer membrane protein (ompU), and central regulatory protein ToxR (toxR) in ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.40.11.4321-4324.2002
更新日期：2002-11-01 00:00:00
abstract：:Klebsiella pneumoniae carbapenemases (KPCs) are considered a serious threat to antibiotic therapy, as they confer resistance to carbapenems, which are used to treat extended-spectrum beta-lactamase (ESBL)-producing bacteria. Here, we describe the development and evaluation of a DNA microarray for the detection and gen...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.00990-12
更新日期：2012-12-01 00:00:00
abstract：:Human milk obtained from 21 American nursing mothers was studied for the presence of secretory immunoglobulin A antibody to rotavirus, the most common etiological agent of infantile gastroenteritis. Antibody was quantitated by adaptation of a recently described solid-phase radioimmunoassay technique that employs simia...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:A slightly modified Moeller medium for amino acid decarboxylase and dihydrolase testing by the replicator method correlated 96 to 99% with the reference Moeller broth method. False positive results were not encountered in this study. ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
abstract：:Sixty-three clinical isolates of charcoal-black-pigmented, gram-positive coryneform rods were received for identification by the Centers for Disease Control and Prevention (CDC) and were provisionally designated CDC fermentative coryneform group 4 (FCG4). Forty-five of these were characterized by morphological, physio...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.42.9.4189-4198.2004
更新日期：2004-09-01 00:00:00
abstract：:Several instruments for automated or semiautomated susceptibility testing are currently available. Three of these instruments, Autobac (General Diagnostics, Warner-Lambert Co., Morris Plains, N.J.), MS-2 (Abbott Laboratories, Dallas, Tex.), and AutoMicrobic system (AMS) (Vitek, Inc., Hazelwood, Mo.) were compared for ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.15.5.902-905.1982
更新日期：1982-05-01 00:00:00
abstract：:Used peritoneal dialysis fluid was collected from patients undergoing continuous ambulatory peritoneal dialysis, and its pH and composition were assessed after incubation in either air or air with 5% CO2. Precipitation of calcium, magnesium, phosphate, and proteins occurred in the dialysis fluid incubated in air at 37...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.28.10.2183-2186.1990
更新日期：1990-10-01 00:00:00
abstract：:A total of 1,298 nonfermentative gram-negative rods were used to evaluate the performance of the individual biochemical tests in the N/F System and to determine whether the observed results included sufficient key reactions for rapid identification. The system included an oxidase test, two screening tubes providing 5 ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1979-02-01 00:00:00
abstract：:Specimens from skin lesions were examined simultaneously for herpes simplex virus (HSV) and varicella-zoster virus (VZV) by direct specimen testing and shell vial culture in single-test systems. For direct testing, cells in a single specimen well were stained with a combination direct-indirect immunofluorescence stain...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.31.12.3260-3263.1993
更新日期：1993-12-01 00:00:00
abstract：:A fluorogenic assay for the detection of beta-glucosidase was developed as part of a simplified conventional method to distinguish Staphylococcus warneri and Staphylococcus hominis isolated from bovine body sites. The assay is based on the fact that strains of S. warneri produce beta-glucosidase, while strains of S. h...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.28.3.602-.1990
更新日期：1990-03-01 00:00:00
abstract：:A simple enzyme immunoassay has been developed to allow the quantitation of the human response to immunization with pneumococcal polysaccharide. The assay uses the 14-valent vaccine (Pneumovax) as a convenient antigen to adsorb to the solid-phase microdilution plate wells and commercially available isotype-specific an...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.26.8.1575-1579.1988
更新日期：1988-08-01 00:00:00
abstract：:In specific-pathogen-free dogs experimentally infected with Borrelia burgdorferi by tick exposure, treatment with high doses of amoxicillin or doxycycline for 30 days diminished but failed to eliminate persistent infection. Although joint disease was prevented or cured in five of five amoxicillin- and five of six doxy...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.35.1.111-116.1997
更新日期：1997-01-01 00:00:00
abstract：:Scedosporium prolificans is a soil saprophyte that is associated with a large variety of infectious processes and with respiratory colonization in immunocompetent and immunocompromised patients. We report the first described case of S. prolificans keratouveitis associated with the intraocular long-term retention of a ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.39.12.4579-4582.2001
更新日期：2001-12-01 00:00:00
abstract：:Antibody to equid herpesvirus 1, which mediates single radial hemolysis, is that responsible for neutralization. Hemagglutination inhibition antibody is not necessarily involved in neutralization or hemolysis. ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1978-05-01 00:00:00
abstract：:Real-time PCR (rt-PCR) is an important diagnostic tool for the identification of Bordetella pertussis, Bordetella holmesii, and Bordetella parapertussis. Most U.S. public health laboratories (USPHLs) target IS481, present in 218 to 238 copies in the B. pertussis genome and 32 to 65 copies in B. holmesii. The CDC devel...
journal_title：Journal of clinical microbiology
pub_type： 历史文章,杂志文章
doi：10.1128/JCM.02368-14
更新日期：2015-01-01 00:00:00
abstract：:Three cloned segments of Mycobacterium tuberculosis DNA which are promising as clinical probes were identified. An MboI digest of DNA from a clinical isolate of M. tuberculosis was cloned into bacteriophage M13. To identify recombinants specific for the M. tuberculosis complex, plaque lifts were hybridized with M. bov...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.26.11.2240-2245.1988
更新日期：1988-11-01 00:00:00
abstract：:Clinical presentations for viral respiratory tract infections are often nonspecific, and a rapid, high-throughput laboratory technique that can detect a panel of common viral pathogens is clinically desirable. We evaluated two multiplex reverse transcription-PCR (RT-PCR) products coupled with microarray-based systems ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.00210-07
更新日期：2007-07-01 00:00:00
abstract：:We report a case where a mite egg found in urine caused diagnostic confusion. The possibility of gut or bladder mite infection should be entertained only after repeated identification of mites in urine or stool samples from a symptomatic patient with no other cause for the symptoms and where the possibilities of conta...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.43.12.6200-6201.2005
更新日期：2005-12-01 00:00:00
abstract：:Extended incubation of culture plates was studied to see if the recovery of Bordetella spp. from nasopharyngeal swabs could be improved. Forty-eight Bordetella isolates were recovered from 103 children (overall positive-culture rate, 46.6%) who met the clinical case definition of pertussis. Seven of 44 (16%) B. pertus...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.34.6.1563-1564.1996
更新日期：1996-06-01 00:00:00
abstract：:To differentiate the Borrelia burgdorferi sensu lato genospecies, LightCycler real-time PCR was used for the fluorescence (SYBR Green I) melting curve analysis of borrelial recA gene PCR products. The specific melting temperature analyzed is a function of the GC/AT ratio, length, and nucleotide sequence of the amplifi...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.38.7.2756-2759.2000
更新日期：2000-07-01 00:00:00
abstract：:To establish the exact pathogenic role of Mycobacterium abscessus in cystic fibrosis (CF), molecular tests are required for accurate identification. Forty-eight M. abscessus isolates from seven patients with CF were analyzed by sequence analysis for sequence variants within the hsp65 gene and the 16S-23S intergenic se...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.43.7.3500-3503.2005
更新日期：2005-07-01 00:00:00
abstract：:The Cobas-Bact (Roche Diagnostics, Basel, Switzerland) new rotor for the identification (ID) in 4 h 20 min of 33 members of the family Enterobacteriaceae to genus and species level was evaluated by testing 444 strains of which 398 belonged to common species and 46 belonged to rare species of Enterobacteriaceae. Each s...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.25.1.61-66.1987
更新日期：1987-01-01 00:00:00
abstract：:The development of a novel normalized quantitative competitive real-time PCR on the LightCycler instrument (NQC-LC-PCR) and its application to the quantification of cytomegalovirus (CMV) DNA in clinical samples are described. A heterologous competitor DNA was spiked into test samples and served as an internal amplific...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.40.12.4547-4553.2002
更新日期：2002-12-01 00:00:00
abstract：:In a large prospective comparison, the illumigene test detected Clostridium difficile in 98% of toxin-positive and 58% of toxin-negative samples confirmed positive by other methods. The Xpert was uniformly sensitive. Most samples with discrepant results had C. difficile concentrations below the illumigene limit of det...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.02120-12
更新日期：2013-01-01 00:00:00
abstract：:Sensitivity of the enzyme-linked immunosorbent assay for detecting serum antibodies to rotavirus was greatly enhanced when rotavirus particles were fragmented by chaotropic agents (NaSCN or guanidine hydrochloride) before adsorption of the antigens to the solid phase. For detecting fecal antibodies, the addition of fe...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.19.2.259-263.1984
更新日期：1984-02-01 00:00:00
abstract：:Diagnosis of invasive aspergillosis (IA) requires increasingly rapid molecular methods that enable sensitive detection and discrimination between species. We designed and evaluated a real-time PCR-based method that combined melting temperature (T(m)) calling analysis of a specific probe with high-resolution melting an...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.00176-12
更新日期：2012-10-01 00:00:00
abstract：:A quantitative modification of the shell vial assay was used to investigate cytomegalovirus viremia in solid-organ transplant recipients. The level of viremia detected in 109 of 407 specimens ranged from 0.02 to 28 infectious foci per 100,000 leukocytes. By using a Poisson model, a technique was developed to determine...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.30.10.2620-2624.1992
更新日期：1992-10-01 00:00:00
abstract：:A new molecular biological approach for the identification of bacteria is described. This approach employs PCR of bacterial cell lysates with conserved primers located in the 16S rRNA sequence flanking a variable region, and analysis of the amplified product was based on the principle of single-strand conformation pol...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.32.12.3002-3007.1994
更新日期：1994-12-01 00:00:00
abstract：:Performance in proficiency testing (PT) schemes is an objective measure of a laboratory's best performance. We examined the performance of participants in two parasitology PT schemes in South Africa from 2004 through 2010. The average rates of acceptable scores over the period were 58% and 66% for the stool and blood ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.01308-12
更新日期：2012-10-01 00:00:00
