Identification of five Peptostreptococcus species isolated predominantly from the female genital tract by using the rapid ID32A system.

abstract：:We have developed and evaluated a new method to quantify human immunodeficiency virus type 2 (HIV-2) proviral DNA based on LightCycler real-time PCR. The assay has a detection limit of 5 copies/10(5) peripheral blood mononuclear cells (PBMC) and is insensitive to HIV-2 strain variability: HIV-2 subtypes A and B are bo...

journal_title：Journal of clinical microbiology

authors： Damond F,Descamps D,Farfara I,Telles JN,Puyeo S,Campa P,Leprêtre A,Matheron S,Brun-Vezinet F,Simon F

更新日期：2001-12-01 00:00:00

abstract：:Viridans streptococci (VS) are common etiologic agents of subacute infective endocarditis and are capable of causing a variety of pyogenic infections. Many species of VS are difficult to differentiate by phenotypic traits. An oligonucleotide array based on 16S-23S rRNA gene intergenic spacer (ITS) sequences was develo...

journal_title：Journal of clinical microbiology

authors： Chen CC,Teng LJ,Kaiung S,Chang TC

更新日期：2005-04-01 00:00:00

abstract：:The distribution of EDL 933 O island 122 (OI-122) was investigated in 70 strains of Verocytotoxin-producing Escherichia coli (VTEC) of multiple serotypes that were classified into five "seropathotypes" (A through E) based on the reported occurrence of serotypes in human disease, in outbreaks, and/or in the hemolytic-u...

journal_title：Journal of clinical microbiology

authors： Karmali MA,Mascarenhas M,Shen S,Ziebell K,Johnson S,Reid-Smith R,Isaac-Renton J,Clark C,Rahn K,Kaper JB

更新日期：2003-11-01 00:00:00

abstract：:Currently, no agar-based susceptibility testing method has been standardized for testing dermatophytes. We describe a newly developed agar-based method employing disk diffusion assay to test the susceptibility of 47 isolates of dermatophytes against 8 antifungals. Our results show that the method is reproducible, is s...

journal_title：Journal of clinical microbiology

authors： Nweze EI,Mukherjee PK,Ghannoum MA

更新日期：2010-10-01 00:00:00

abstract：:A biotin-avidin-amplified indirect immunofluorescence method was used to demonstrate specific serum immunoglobulin M (IgM) antibodies in nephropathia epidemica, the Scandinavian type of hemorrhagic fever with renal syndrome. The antigen in the test was the cross-reacting agent of Korean hemorrhagic fever, Hantaan viru...

journal_title：Journal of clinical microbiology

authors： Settergren B,Juto P,Wadell G

更新日期：1987-06-01 00:00:00

abstract：:Roche Molecular Systems recently released two PCR-based assays, AMPLICOR and LINEAR ARRAY (LA), for the detection and genotyping, respectively, of human papillomaviruses (HPVs). The manual specimen processing method recommended for use with both assays, AmpliLute, can be time-consuming and labor-intensive and is open ...

journal_title：Journal of clinical microbiology

authors： Stevens MP,Rudland E,Garland SM,Tabrizi SN

更新日期：2006-07-01 00:00:00

abstract：:Gram-negative, anaerobic bacilli are unusual organisms to be isolated in cases of acute septic arthritis. We report the isolation of Fusobacterium necrophorum from joint aspirate in a case of acute septic arthritis, which presented 3 weeks after the drainage of a dental abscess. ...

journal_title：Journal of clinical microbiology

authors： Sonsale PD,Philipson MR,Bowskill J

更新日期：2004-07-01 00:00:00

abstract：:The IDI-MRSA assay (Infectio Diagnostic, Inc., Sainte-Foy, Quebec, Canada) with the Smart Cycler II rapid DNA amplification system (Cepheid, Sunnyvale, CA) appears to be sensitive and specific for the rapid detection of nasal colonization by methicillin-resistant Staphylococcus aureus (MRSA). We assessed the sensitivi...

journal_title：Journal of clinical microbiology

authors： Bishop EJ,Grabsch EA,Ballard SA,Mayall B,Xie S,Martin R,Grayson ML

更新日期：2006-08-01 00:00:00

abstract：:The Gen-Probe AMPLIFIED Chlamydia Trachomatis Assay (AMP CT) uses transcription-mediated amplification and hybridization protection assay procedures to qualitatively detect Chlamydia trachomatis rRNA in urine, endocervical swab, and urethral specimens. The performance of the AMP CT was compared to that of cell culture...

journal_title：Journal of clinical microbiology

authors： Ferrero DV,Meyers HN,Schultz DE,Willis SA

更新日期：1998-11-01 00:00:00

abstract：:Therapeutic options for infections caused by gram-negative organisms expressing plasmid-mediated AmpC beta-lactamases are limited because these organisms are usually resistant to all the beta-lactam antibiotics, except for cefepime, cefpirome, and the carbapenems. These organisms are a major concern in nosocomial infe...

journal_title：Journal of clinical microbiology

authors： Pérez-Pérez FJ,Hanson ND

更新日期：2002-06-01 00:00:00

abstract：:A long-term outbreak of urinary tract-associated multiply resistant Providencia stuartii occurred in a large medical facility that included a 513-bed chronic care unit. The unique characteristics of this outbreak were that from within a single medical facility, P. stuartii with multiple serotypes, biotypes, and antibi...

journal_title：Journal of clinical microbiology

authors： Kocka FE,Srinivasan S,Mowjood M,Kantor HS

更新日期：1980-02-01 00:00:00

abstract：:Members of the genus Abiotrophia, formerly known as nutritionally variant streptococci, are important pathogens causing septicemia and endocarditis. Cultivation and biochemical differentiation of Abiotrophia spp. are often difficult. Based on 16S rRNA sequences, two PCR assays for detection and identification of Abiot...

journal_title：Journal of clinical microbiology

authors： Roggenkamp A,Leitritz L,Baus K,Falsen E,Heesemann J

更新日期：1998-10-01 00:00:00

abstract：:The slightly revised, current scheme of 20 flagellar (H) antigens of Serratia marcesens was examined. The seven new H antigens were demonstrated to be antigenically distinct as determined with Le Minor's H-immobilization test. The H-immobilization antibodies of rabbit anti-H immune sera proved resistant to treatment w...

journal_title：Journal of clinical microbiology

authors： Traub WH,Fukushima PI

更新日期：1979-07-01 00:00:00

abstract：:Suspected outbreaks caused by pseudomonas aeruginosa in 19 hospitals and two motels were studied. On the basis of epidemiological analysis, serological typing, and antibiotic resistance patterns, 17 were classified as single-strain outbreaks. Six were classified as common-source outbreaks: of these, three were caused ...

journal_title：Journal of clinical microbiology

authors： Farmer JJ 3rd,Weinstein RA,Zierdt CH,Brokopp CD

更新日期：1982-08-01 00:00:00

abstract：:One of the capabilities developed by bacteria is the ability to gain large fragments of DNA from other bacteria or to lose portions of their own genomes. Among these exchangeable fragments are the genomic islands (GIs). Nine GIs have been identified in Brucella, and genomic island 3 (GI-3) is shared by two pathogenic ...

journal_title：Journal of clinical microbiology

authors： Céspedes S,Salgado P,Valenzuela P,Vidal R,Oñate AA

更新日期：2011-07-01 00:00:00

abstract：:Growth curves of the yeast form of Paracoccidioides brasiliensis B-339 based on total and viable cell counts were determined. Crude culture filtrate antigens were obtained after 7, 10, 15, 20, 25, and 30 days of incubation. Different patterns of proteins were obtained by affinity chromatography on Sepharose 4B-immunog...

journal_title：Journal of clinical microbiology

authors： De Camargo Z,Unterkircher C,Campoy SP,Travassos LR

更新日期：1988-10-01 00:00:00

abstract：:The antigenic cross-reactive characteristics of herpes B virus and herpes simplex virus (HSV) type 1 (HSV-1) and HSV-2 are responsible for false-positive diagnoses by serological assays in humans and macaques. In the present study, we developed a fluorometric indirect enzyme-linked immunosorbent assay (ELISA) with rec...

journal_title：Journal of clinical microbiology

authors： Fujima A,Ochiai Y,Saito A,Omori Y,Noda A,Kazuyama Y,Shoji H,Tanabayashi K,Ueda F,Yoshikawa Y,Hondo R

更新日期：2008-01-01 00:00:00

abstract：:We surveyed 75 clinical laboratories to determine if National Committee for Clinical Laboratory Standards (NCCLS) were being used for the susceptibility testing of Haemophilus influenzae. Of the 66 laboratories that performed susceptibility testing, all claimed to follow current NCCLS guidelines. However, upon further...

journal_title：Journal of clinical microbiology

authors： Scriver SR,Hoban DJ,McGeer A,Moore TC,Walmsley SL,Low DE

更新日期：1994-08-01 00:00:00

abstract：:A 4-h Micro-ID technique for direct identification of oxidase-negative gram-negative rods from positive blood cultures was compared to subculture and species identification of single colonies by API 20E and Micro-ID, using standardized inocula. A total of 127 patients (220 positive cultures) were studied. Isolates inc...

journal_title：Journal of clinical microbiology

authors： Appelbaum PC,Schick SF,Kellogg JA

更新日期：1980-10-01 00:00:00

abstract：:Reactivation of latent varicella-zoster virus (VZV), presenting as localized zoster or as disseminated infection, is a common and potentially serious complication in hematopoietic stem cell transplantation (HSCT) recipients. We retrospectively studied anti-VZV immunoglobulin G titers by the immune adherence hemaggluti...

journal_title：Journal of clinical microbiology

authors： Onozawa M,Hashino S,Takahata M,Fujisawa F,Kawamura T,Nakagawa M,Kahata K,Kondo T,Ota S,Tanaka J,Imamura M,Asaka M

更新日期：2006-12-01 00:00:00

abstract：:Real-time isothermal nucleic acid sequence-based amplification (RT-NASBA) was applied to the detection of Mycoplasma pneumoniae. In vitro-generated M. pneumoniae RNA was used to assess the sensitivity of the assay. The 95% hit rate was 148 molecules of M. pneumoniae RNA in the amplification and 10(4) molecules of in v...

journal_title：Journal of clinical microbiology

authors： Loens K,Ieven M,Ursi D,Beck T,Overdijk M,Sillekens P,Goossens H

更新日期：2003-09-01 00:00:00

abstract：:Direct tube coagulase testing for identification of Staphylococcus aureus from BACTEC culture broth showed a sensitivity, a specificity, and positive and negative predicative values of 34%, 100%, 100%, and 80.2% with 2 h of incubation and 65%, 98.7, 99.7%, and 88.6% with 4 h of incubation. Anaerobic blood culture cont...

journal_title：Journal of clinical microbiology

authors： Qian Q,Eichelberger K,Kirby JE

更新日期：2007-07-01 00:00:00

abstract：:BHI agars supplemented with vancomycin 4 (BHI-V4) and 3 (BHI-V3) mg/liter have been proposed for screening vancomycin intermediately susceptible Staphylococcus aureus (VISA) and heteroresistant (hVISA) phenotypes, respectively, but growth interpretation criteria have not been established. We reviewed the growth result...

journal_title：Journal of clinical microbiology

authors： Khatib R,Riederer K,Sharma M,Shemes S,Iyer SP,Szpunar S

更新日期：2015-11-01 00:00:00

abstract：:A new liquid medium (modified Lombard-Dowell broth) was inoculated with stock culture strains of aerobic and anaerobic bacteria and compared with prereduced chopped-meat glucose inoculated with the same anaerobes. Both broths were subcultured at 48 and 72 h to aerobic and anaerobic blood agar plates, and the numbers o...

journal_title：Journal of clinical microbiology

authors： Jessee MT,Robinson PJ

更新日期：1977-11-01 00:00:00

abstract：:This study evaluates the performance of a PCR assay for the detection of Pneumocystis carinii from respiratory specimens that has been designed for use in the clinical microbiology laboratory. The test includes a simple method for nucleic acid extraction and amplification, a colorimetric probe hybridization technique ...

journal_title：Journal of clinical microbiology

authors： Caliendo AM,Hewitt PL,Allega JM,Keen A,Ruoff KL,Ferraro MJ

更新日期：1998-04-01 00:00:00

abstract：:A noncompetitive enzyme-linked immunosorbent assay that enables the quantitation of toxic shock syndrome toxin 1 (TSST-1) to as little as 30 pg/ml and the detection of TSST-1 to 10 pg/ml in phosphate-buffered saline including 33% human serum or plasma was developed. It takes only 3 h to complete this assay after plate...

journal_title：Journal of clinical microbiology

authors： Miwa K,Fukuyama M,Kunitomo T,Igarashi H

更新日期：1994-02-01 00:00:00

abstract：:Scedosporium prolificans is a soil saprophyte that is associated with a large variety of infectious processes and with respiratory colonization in immunocompetent and immunocompromised patients. We report the first described case of S. prolificans keratouveitis associated with the intraocular long-term retention of a ...

journal_title：Journal of clinical microbiology

authors： Arthur S,Steed LL,Apple DJ,Peng Q,Howard G,Escobar-Gomez M

更新日期：2001-12-01 00:00:00

abstract：:Early and accurate diagnosis is essential for optimal therapeutic outcomes in patients infected with HIV. Currently, none of the commercially available fourth-generation assays differentiate HIV-1 and HIV-2 antibodies (Ab) or the HIV-1 p24 antigen (Ag). The aim of this study was to evaluate the performance of a novel ...

journal_title：Journal of clinical microbiology

authors： Salmona M,Delarue S,Delaugerre C,Simon F,Maylin S

更新日期：2014-01-01 00:00:00

abstract：:An unusual Enterocytozoon bieneusi genotype was found in seven guinea pigs and a 2-year-old child in the same household. The genetic uniqueness of the parasite, its wide occurrence in other guinea pigs in the community, and its absence in other children in the community suggest the possibility of zoonotic transmission...

journal_title：Journal of clinical microbiology

authors： Cama VA,Pearson J,Cabrera L,Pacheco L,Gilman R,Meyer S,Ortega Y,Xiao L

更新日期：2007-08-01 00:00:00

abstract：:Five serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (RBT), complement fixation test (CFT), radial immunodiffusion (RID) with Brucella and Yersinia enterocolitica O:9 polysaccharides, counterimmunoelectrophoresis (CIEP) with cytosol, and enzyme-linked immunosorbent ass...

journal_title：Journal of clinical microbiology

authors： Díaz-Aparicio E,Marín C,Alonso-Urmeneta B,Aragón V,Pérez-Ortiz S,Pardo M,Blasco JM,Díaz R,Moriyón I

更新日期：1994-05-01 00:00:00