First report of Nocardia beijingensis infection in an immunocompetent host in the United States.

abstract：:The development of a rhinovirus (RV)-RNA-specific reverse transcription (RT)-PCR assay is complicated by the close homology between the RV and enterovirus (EV) genomes in the highly conserved 5'-noncoding region, which is chosen for primer design in most RT-PCR assays. We have developed a sensitive, rapid, and RV-spec...

journal_title：Journal of clinical microbiology

authors： Steininger C,Aberle SW,Popow-Kraupp T

更新日期：2001-01-01 00:00:00

abstract：:The frequencies of 10 opportunistic DNA viruses were determined by multiplex real-time PCR in paired cerebrospinal fluid (CSF) and brain tissue of HIV-infected individuals. In the CSF, viruses were detectable in 45/55 cases: JC virus (JCV) in 62%, Epstein-Barr virus (EBV) in 44%, cytomegalovirus (CMV) in 25%, varicell...

journal_title：Journal of clinical microbiology

authors： Kannangai R,Sachithanandham J,Mahadevan A,Abraham AM,Sridharan G,Desai A,Ravi V,Shankar SK

更新日期：2013-03-01 00:00:00

abstract：:To apply cDNA-RNA hybridization methods to the detection of hepatitis A virus (HAV) in clinical materials, we developed a two-step method in which a microtiter-based, solid-phase immunoadsorption procedure incorporating a monoclonal anti-HAV capture antibody was followed by direct blotting of virus eluates to nitrocel...

journal_title：Journal of clinical microbiology

authors： Jansen RW,Newbold JE,Lemon SM

更新日期：1985-12-01 00:00:00

abstract：:A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae. Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates. Forty distinct allelic profiles were identified. MLST data were validated against ribotyp...

journal_title：Journal of clinical microbiology

authors： Diancourt L,Passet V,Verhoef J,Grimont PA,Brisse S

更新日期：2005-08-01 00:00:00

abstract：:Paracoccidioides brasiliensis isolates from 10 nine-banded armadillos (Dasypus novemcinctus) were comparable with 19 clinical isolates by sequence analysis of the PbGP43 gene and ribosomal internal transcribed spacer 1 (ITS1) and ITS2 and by random amplified polymorphic DNA. In this original ITS study, eight isolates ...

journal_title：Journal of clinical microbiology

authors： Hebeler-Barbosa F,Morais FV,Montenegro MR,Kuramae EE,Montes B,McEwen JG,Bagagli E,Puccia R

更新日期：2003-12-01 00:00:00

abstract：:A multiplex method using the SNaPshot technique was developed to screen for six common mycobacterial species: Mycobacteria tuberculosis, M. avium, M. intracellulare, M. chelonae, M. kansasii, and M. gordonae. A total of 468 mycobacterial clinical isolates were subjected to analysis for the presence of the six mycobact...

journal_title：Journal of clinical microbiology

authors： Wang H,Yue J,Han M,Yang J,Zhao Y

更新日期：2010-01-01 00:00:00

abstract：:Eleven isolates representing five distinct outbreaks of pneumococcal conjunctivitis were examined for phenotypic and genetic characteristics. None of the strains possessed capsules, and all strains were susceptible to optochin, bile soluble, and Gen-Probe AccuProbe test positive. All 11 isolates were confirmed as Stre...

journal_title：Journal of clinical microbiology

authors： Carvalho MG,Steigerwalt AG,Thompson T,Jackson D,Facklam RR

更新日期：2003-09-01 00:00:00

abstract：:The development of porcine epidemic diarrhea virus (PEDV) antibody-based assays is important for detecting infected animals, confirming previous virus exposure, and monitoring sow herd immunity. However, the potential cross-reactivity among porcine coronaviruses is a major concern for the development of pathogen-speci...

journal_title：Journal of clinical microbiology

authors： Gimenez-Lirola LG,Zhang J,Carrillo-Avila JA,Chen Q,Magtoto R,Poonsuk K,Baum DH,Piñeyro P,Zimmerman J

更新日期：2017-05-01 00:00:00

abstract：:A commercially available endotoxin assay (CS-TAL) employing a chromogenic peptide and an amebocyte lysate from the Japanese horseshoe crab, Tachypleus tridentatus, gave a positive result with aqueous extracts of all 15 strains of Candida albicans and 1 strain each of Candida tropicalis, Cryptococcus neoformans, and a ...

journal_title：Journal of clinical microbiology

authors： Hodes DS,Heon D,Hass A,Hyatt AC,Hodes HL

更新日期：1987-09-01 00:00:00

abstract：:The NucliSENS easyMAG automated system was compared to the column-based Qiagen method for Epstein-Barr virus (EBV) or cytomegalovirus (CMV) DNA extraction from whole blood before viral load determination using the corresponding R-gene amplification kits. Both extraction techniques exhibited a total agreement of 81.3% ...

journal_title：Journal of clinical microbiology

authors： Pillet S,Bourlet T,Pozzetto B

更新日期：2009-11-01 00:00:00

abstract：:A survey for carriers of Streptococcus pneumoniae among 573 children was undertaken. Throat and nasopharyngeal cultures from 303 children disclosed pneumococci in 89 (29.4%) nasopharyngeal swabs but in only 8 (2.6%) throat specimens. Carriers of pneumococci were detected with frequencies of 84.4 and 79.9% on aerobical...

journal_title：Journal of clinical microbiology

authors： Robins-Browne RM,Kharsany AB,Ramsaroop UG

更新日期：1982-07-01 00:00:00

abstract：:Since 2003, three novel genotypes of H1 influenza viruses have been recovered from Canadian pigs, including a wholly human H1N2 virus and human-swine reassortants. These isolates demonstrate that human-lineage H1N2 viruses are infectious for pigs and that viruses with a human PB1/swine PA/swine PB2 polymerase complex ...

journal_title：Journal of clinical microbiology

authors： Karasin AI,Carman S,Olsen CW

更新日期：2006-03-01 00:00:00

abstract：:The emergence of ciprofloxacin-resistant Salmonella enterica serovar Choleraesuis in recent years has become an important public health issue in Taiwan. The resistant strains that cause human infections are considered to be from pigs. In this study, we characterized 157 swine and 42 human Salmonella serovar Choleraesu...

journal_title：Journal of clinical microbiology

authors： Chang CC,Lin YH,Chang CF,Yeh KS,Chiu CH,Chu C,Chien MS,Hsu YM,Tsai LS,Chiou CS

更新日期：2005-06-01 00:00:00

abstract：:Discovery of bats with severe acute respiratory syndrome (SARS)-related coronaviruses (CoVs) raised the specter of potential future outbreaks of zoonotic SARS-CoV-like disease in humans, which largely went unheeded. Nevertheless, the novel SARS-CoV-2 of bat ancestral origin emerged to infect humans in Wuhan, China, in...

journal_title：Journal of clinical microbiology

authors： Saif LJ,Jung K

更新日期：2020-07-23 00:00:00

abstract：:Porphyromonas gingivalis is one of the major causative agents of adult periodontitis. One of the features of this periodontal pathogen is its ability to attach to a variety of oral bacterial surfaces and to colonize subgingival dental plaque. We have shown that Streptococcus cristatus CC5A inhibits expression of fimA,...

journal_title：Journal of clinical microbiology

authors： Wang BY,Wu J,Lamont RJ,Lin X,Xie H

更新日期：2009-12-01 00:00:00

abstract：:The binding of human immunoglobulin G by Fc receptors in herpes simplex virus (HSV)-infected cells can cause false-positive interpretations in the immunofluorescence test for HSV antibody. When the infected cell smears were treated with 10% glacial acetic acid for 5 min and rinsed in phosphate-buffered saline before t...

journal_title：Journal of clinical microbiology

authors： Gallo D

更新日期：1986-10-01 00:00:00

abstract：:Few studies have assessed the time to blood culture positivity as a predictor of clinical outcome in bloodstream infections (BSIs). The purpose of this study was to evaluate the time to positivity (TTP) of blood cultures in patients with Staphylococcus aureus BSIs and to assess its impact on clinical outcome. We perfo...

journal_title：Journal of clinical microbiology

authors： Marra AR,Edmond MB,Forbes BA,Wenzel RP,Bearman GM

更新日期：2006-04-01 00:00:00

abstract：:Helicobacter pylori strains display remarkable genetic diversity, and the presence of strains bearing the toxigenic vacA s1 allele, a complete cag pathogenicity island (PAI), cagA alleles containing multiple EPIYA phosphorylation sites, and expressing the BabA adhesin correlates with development of gastroduodenal dise...

journal_title：Journal of clinical microbiology

authors： Talarico S,Gold BD,Fero J,Thompson DT,Guarner J,Czinn S,Salama NR

更新日期：2009-06-01 00:00:00

abstract：:Detection of methicillin (meticillin)-resistant Staphylococcus aureus colonization was assessed using combined nose and groin swabs in two commercial PCR assays (the Xpert MRSA assay and the BD GeneOhm MRSA assay). Compared to routine culture, both had similar sensitivities (87.0% versus 84.8%, respectively) and speci...

journal_title：Journal of clinical microbiology

authors： Kelley PG,Grabsch EA,Howden BP,Gao W,Grayson ML

更新日期：2009-11-01 00:00:00

abstract：:The causes of sarcoidosis are unknown. In this study, we report the presence of Mycobacterium tuberculosis complex and Propionibacterium granulosum DNA in a significant proportion of Greek patients with sarcoidosis. Human herpesvirus 8 DNA was not detected in sarcoid tissues from Greek patients. Our findings are discu...

journal_title：Journal of clinical microbiology

authors： Gazouli M,Ikonomopoulos J,Trigidou R,Foteinou M,Kittas C,Gorgoulis V

更新日期：2002-08-01 00:00:00

abstract：:Human milk obtained from 21 American nursing mothers was studied for the presence of secretory immunoglobulin A antibody to rotavirus, the most common etiological agent of infantile gastroenteritis. Antibody was quantitated by adaptation of a recently described solid-phase radioimmunoassay technique that employs simia...

journal_title：Journal of clinical microbiology

authors： Cukor G,Blacklow NR,Capozza FE,Panjvani ZF,Bednarek F

更新日期：1979-01-01 00:00:00

abstract：:A species-specific molecular beacon real-time PCR assay was developed for rapid diagnosis of Exserohilum rostratum infection. As low as 100 fg of E. rostratum DNA can be reliably detected in the presence of 50 ng of human DNA, with a dynamic linear quantification range from 20 ng to 200 fg. ...

journal_title：Journal of clinical microbiology

authors： Zhao Y,Petraitiene R,Walsh TJ,Perlin DS

更新日期：2013-03-01 00:00:00

abstract：:A new PCR-reverse dot blot hybridization (RDBH) assay was developed for the rapid identification of Mycobacterium species in clinical isolates. The assay, which targets the 16S rRNA, was evaluated for 27 mycobacterial reference strains and 340 clinical isolates that were simultaneously identified by DNA sequencing and...

journal_title：Journal of clinical microbiology

authors： Wu X,Zhang J,Liang J,Lu Y,Li H,Li C,Yue J,Zhang L,Liu Z

更新日期：2007-06-01 00:00:00

abstract：:We report a case in which fecal microbiota transplantation (FMT) utilized for relapsing Clostridium difficile colitis successfully eradicated colonization with several multidrug-resistant organisms (MDROs). FMT may have an additive benefit of reducing MDRO carriage and should be further investigated as a potential mea...

journal_title：Journal of clinical microbiology

authors： Crum-Cianflone NF,Sullivan E,Ballon-Landa G

更新日期：2015-06-01 00:00:00

abstract：:Rhodococcus equi infection is increasing in regions with high HIV prevalence worldwide. The microbiological features and clinical mimicry of tuberculosis infection pose diagnostic challenges in high-tuberculosis-incidence settings. We present two HIV-associated cases of R. equi infection from Vietnam and discuss the u...

journal_title：Journal of clinical microbiology

authors： Le T,Cash-Goldwasser S,Tho PV,Lan NP,Campbell JI,van Doorn HR,Lam NT,Trung NV,Trinh DT,Van Kinh N,Wertheim HF

更新日期：2015-04-01 00:00:00

abstract：:The development and application of nucleic acid sequence-based amplification (NASBA) assays for the detection of West Nile (WN) and St. Louis encephalitis (SLE) viruses are reported. Two unique detection formats were developed for the NASBA assays: a postamplification detection step with a virus-specific internal capt...

journal_title：Journal of clinical microbiology

authors： Lanciotti RS,Kerst AJ

更新日期：2001-12-01 00:00:00

abstract：:The QuantiFERON-TB Gold Plus (QFT-Plus; Qiagen, Germantown, MD) interferon gamma release assay (IGRA) received FDA clearance in 2017 and will replace the prior version of the assay, the QFT-Gold In-Tube (QFT-GIT). Here, we compared performances of the QFT-Plus assay and the QFT-GIT version in a diverse patient populat...

journal_title：Journal of clinical microbiology

authors： Theel ES,Hilgart H,Breen-Lyles M,McCoy K,Flury R,Breeher LE,Wilson J,Sia IG,Whitaker JA,Clain J,Aksamit TR,Escalante P

更新日期：2018-06-25 00:00:00

abstract：:Nontuberculous mycobacterial infections caused by Mycobacterium abscessus are responsible for a range of disease manifestations from pulmonary to skin infections and are notoriously difficult to treat, due to innate resistance to many antibiotics. Previous population studies of clinical M. abscessus isolates utilized ...

journal_title：Journal of clinical microbiology

authors： Davidson RM,Hasan NA,Reynolds PR,Totten S,Garcia B,Levin A,Ramamoorthy P,Heifets L,Daley CL,Strong M

更新日期：2014-10-01 00:00:00

abstract：:A polymerase chain reaction (PCR) procedure coupled to an enzyme-linked oligonucleotide sorbent assay (ELOSA; a PCR-ELOSA) identified all 24 serotypic variants of bluetongue virus (BTV) without identifying any of six viruses belonging to the related epizootic hemorrhagic disease virus serogroup. The PCR-ELOSA detected...

journal_title：Journal of clinical microbiology

authors： Katz JB,Alstad AD,Gustafson GA,Moser KM

更新日期：1993-11-01 00:00:00

abstract：:A surveillance network was implemented by the Istituto Superiore di Sanità of Rome in collaboration with laboratories of virology in Czech Republic, Slovenia, Croatia, Albania, and Bulgaria. About 1,500 rotavirus-positive stool samples were collected from children with severe gastroenteritis admitted to hospitals or o...

journal_title：Journal of clinical microbiology

authors： Tcheremenskaia O,Marucci G,De Petris S,Ruggeri FM,Dovecar D,Sternak SL,Matyasova I,Dhimolea MK,Mladenova Z,Fiore L,Rotavirus Study Group.

更新日期：2007-07-01 00:00:00