Quantitative competitive PCR with bronchoalveolar lavage fluid for diagnosis of toxoplasmosis in AIDS patients.


:Of 121 AIDS patients, 12 (10%) had Toxoplasma gondii DNA detected by competitive PCR in their bronchoalveolar lavage samples. Quantitation of the PCR results showed a correlation between the parasite burden and the serum lactic dehydrogenase titer. Quantitative competitive PCR could be useful to assess the diagnosis and the severity of pulmonary toxoplasmosis.


J Clin Microbiol


Bretagne S,Costa JM,Fleury-Feith J,Poron F,Dubreuil-Lemaire ML,Vidaud M




Has Abstract


1995-06-01 00:00:00












  • Comparative evaluation of nonradiometric BACTEC and improved oxoid signal blood culture systems in a clinical laboratory.

    abstract::The BACTEC NR660 blood culture system, which uses infrared spectroscopy to detect carbon dioxide generated by bacterial growth, was compared with the new medium formulation of the Oxoid Signal system. Two trials were conducted: a comparative study of 88 organisms in simulated blood cultures and a clinical trial of 3,3...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Daley C,Lim I,Modra J,Wilkinson I

    更新日期:1990-07-01 00:00:00

  • Encephalitis caused by Chikungunya virus in a traveler from the Kingdom of Tonga.

    abstract::Febrile travelers from countries with unique endemic pathogens pose a significant diagnostic challenge. In this report, we describe the case of a Tongan man presenting with fever, rash, and altered mental status. The diagnosis of Chikungunya encephalitis was made using a laboratory-developed real-time RT-PCR and serol...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Nelson J,Waggoner JJ,Sahoo MK,Grant PM,Pinsky BA

    更新日期:2014-09-01 00:00:00

  • Identification of aerobic Gram-positive bacilli by use of Vitek MS.

    abstract::The accuracy of Vitek MS mass spectrometric identifications was assessed for 206 clinically significant isolates of aerobic Gram-positive bacilli representing 20 genera and 38 species. The Vitek MS identifications were correct for 85% of the isolates (56.3% to the species level, 28.6% limited to the genus level), with...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Navas M,Pincus DH,Wilkey K,Sercia L,LaSalvia M,Wilson D,Procop GW,Richter SS

    更新日期:2014-04-01 00:00:00

  • A rapid and high-throughput screening approach for methicillin-resistant Staphylococcus aureus based on the combination of two different real-time PCR assays.

    abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen that has been responsible for major nosocomial epidemics worldwide. For infection control programs, rapid and adequate detection of MRSA is of great importance. We developed a rapid and high-throughput molecular screening approach that consist...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Nijhuis RH,van Maarseveen NM,van Hannen EJ,van Zwet AA,Mascini EM

    更新日期:2014-08-01 00:00:00

  • Improved method for the routine identification of toxigenic Escherichia coli by DNA amplification of a conserved region of the heat-labile toxin A subunit.

    abstract::This report describes a DNA amplification procedure for routine identification of heat-labile-toxin-producing Escherichia coli. Two oligonucleotide primers were used in a polymerase chain reaction procedure to amplify a highly conserved region of the A subunit of the heat-labile enterotoxin gene. Amplifications were d...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Victor T,du Toit R,van Zyl J,Bester AJ,van Helden PD

    更新日期:1991-01-01 00:00:00

  • Multilocus sequence typing analysis of Clostridium perfringens isolates from necrotic enteritis outbreaks in broiler chicken populations.

    abstract::Clostridium perfringens is an important pathogen of animals and humans and is the causative agent of necrotic enteritis (NE) in poultry. This study focuses on the typing of intestinal C. perfringens isolates (n = 61) from outbreaks of NE collected from several areas of Southern Ontario, using a recently developed mult...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Chalmers G,Bruce HL,Hunter DB,Parreira VR,Kulkarni RR,Jiang YF,Prescott JF,Boerlin P

    更新日期:2008-12-01 00:00:00

  • Studies of epidemiology and seroprevalence of bovine noroviruses in Germany.

    abstract::Jena virus (JV) is a bovine enteric calicivirus that causes diarrhea in calves. The virus is approximately 30 nm in diameter and has a surface morphology similar to the human Norwalk virus. The genome sequence of JV was recently described, and the virus has been assigned to the genus Norovirus of the family CALICIVIRI...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Deng Y,Batten CA,Liu BL,Lambden PR,Elschner M,Günther H,Otto P,Schnürch P,Eichhorn W,Herbst W,Clarke IN

    更新日期:2003-06-01 00:00:00

  • Diagnostic Accuracy of Seven Commercial Assays for Rapid Detection of Group A Rotavirus Antigens.

    abstract::Seven commercial immunochromatographic assays intended for the detection of group A rotavirus antigens in human stool samples were evaluated. These assays showed similar levels of diagnostic accuracy and were suitable for the detection of rotavirus in patients with acute gastroenteritis but missed some asymptomatic ro...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kaplon J,Fremy C,Pillet S,Mendes Martins L,Ambert-Balay K,Aho SL,Pothier P

    更新日期:2015-11-01 00:00:00

  • Systematic investigation of enrichment media for wild-type Campylobacter jejuni strains.

    abstract::Of the media examined, thioglycolate broth supplemented with 5% lysed sheep blood, Butzler antibiotic mixture, and 0.1% lauryl sulfate was the most sensitive enrichment medium for recovery of wild-type strains of Campylobacter jejuni from cecal contents of chickens and chicken livers. It allowed the retrieval of 1 CFU...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Barot MS,Bokkenheuser VD

    更新日期:1984-07-01 00:00:00

  • Genetic background affects stability of mecA in Staphylococcus aureus.

    abstract::The staphylococcal methicillin resistance determinant, mecA, resides on a mobile genetic element, staphylococcus chromosomal cassette mec (SCCmec). The distribution of SCCmec in nature is limited to relatively few clonal complexes of related methicillin-resistant Staphylococcus aureus (MRSA). We have previously report...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Katayama Y,Robinson DA,Enright MC,Chambers HF

    更新日期:2005-05-01 00:00:00

  • Multicenter Evaluation of the Etest Gradient Diffusion Method for Ceftolozane-Tazobactam Susceptibility Testing of Enterobacteriaceae and Pseudomonas aeruginosa.

    abstract::Ceftolozane-tazobactam (C/T) is a novel beta-lactam-beta-lactamase inhibitor combination antibiotic approved by the U.S. Food and Drug Administration in 2014 for the treatment of complicated intra-abdominal infections (in combination with metronidazole) and complicated urinary tract infections. In this study, we evalu...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究


    authors: Bailey AL,Armstrong T,Dwivedi HP,Denys GA,Hindler J,Campeau S,Traczewski M,Humphries R,Burnham CA

    更新日期:2018-08-27 00:00:00

  • Emergence of Cryptococcus gattii in a novel environment provides clues to its incubation period.

    abstract::Cryptococcus gattii emerged in 1999 in a distinct ecologic environment on Vancouver Island, Canada. Estimates of incubation period were derived from an analysis of travelers with discreet exposures to this region. Individual case incubation periods ranged from 2 to 11 months, with a median of 6 to 7 months. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: MacDougall L,Fyfe M

    更新日期:2006-05-01 00:00:00

  • Direct identification of Mycobacterium species in Bactec 7H12B medium by gas-liquid chromatography.

    abstract::Twenty-nine Mycobacterium reference strains representing 10 species and 60 mycobacterial cultures isolated from sputum specimens were studied. These cultures were grown in Bactec 7H12B medium (Becton Dickinson and Co., Paramus, N.J.) supplemented with oleic acid-albumin-dextrose-catalase enrichment broth (Becton Dicki...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Chou S,Chedore P,Haddad A,Paul NR,Kasatiya S

    更新日期:1996-05-01 00:00:00

  • Development of a Plasmodium PCR for monitoring efficacy of antimalarial treatment.

    abstract::We report in this work a highly sensitive and nonradioactive PCR method for the detection of the four species of parasite causing human malaria. Plasmodium-specific primers corresponding to the small-subunit rRNA genes of the malaria parasite were used, and a 291-bp fragment was amplified. Our results showed a high sp...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Ciceron L,Jaureguiberry G,Gay F,Danis M

    更新日期:1999-01-01 00:00:00

  • Emergence of a novel Shigella flexneri serotype 4s strain that evolved from a serotype X variant in China.

    abstract::This paper describes the first isolation of a new Shigella flexneri serotype, designated 4s, in Beijing, China. Genotypic and phenotypic profiling suggests that this isolate is a clone of the S. flexneri serotype X variant reference strain. Of particular concern is the multidrug resistance exhibited by this isolate. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Qiu S,Wang Z,Chen C,Liu N,Jia L,Liu W,Wang L,Hao R,Zhang L,Wang Y,Song H

    更新日期:2011-03-01 00:00:00

  • Stability of cervical specimens in SurePath medium for human papillomavirus testing with the Roche cobas 4800 assay.

    abstract::The stability of cervical specimens in SurePath preservative fluid for human papillomavirus (HPV) testing with Roche cobas 4800 was determined using a panel of 308 pooled specimens from a colposcopy referral population. The SurePath specimens appeared to be stable for up to 10 weeks at ambient temperature for HPV test...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Gilbert L,Oates E,Ratnam S

    更新日期:2013-10-01 00:00:00

  • Normalized enzyme-linked immunosorbent assay for determining immunoglobulin G antibodies to cytomegalovirus.

    abstract::A normalized enzyme-linked immunosorbent assay for the determination of immunoglobulin G antibodies to cytomegalovirus is described. The rapid assay involves three 30-min incubations and permits the quantitation of antibody levels with a single-specimen dilution in conjunction with a reference antibody preparation. Th...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kiefer DJ,Phelps DA,Halbert SP

    更新日期:1983-07-01 00:00:00

  • Serological testing for hepatitis B in male homosexuals: special emphasis on hepatitis B e antigen and antibody by radioimmunoassay.

    abstract::Serological markers for hepatitis B virus in male homosexuals demonstrated a high prevalence of past and present infection. Seropositivity of 91% for hepatitis B e antigen or antibody was demonstrated by radioimmunoassay in hepatitis B surface antigen-positive specimens. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Murphy BL,Schreeder MT,Maynard JE,Hadler SC,Sheller MJ

    更新日期:1980-03-01 00:00:00

  • Predicted configurations of oligosaccharide extensions in the lipooligosaccharide of nontypeable Haemophilus influenzae isolates.

    abstract::Lipooligosaccharide configurations were predicted in nontypeable Haemophilus influenzae isolates based on the presence of seven oligosaccharide extension-initiating genes (or alleles). Predicted configurations with 2 to 3 oligosaccharide extensions were more prevalent among middle ear than throat strains. In addition,...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: McCrea KW,Xie J,Daniel D,Ulrich-Lewis JT,Zhang L

    更新日期:2014-07-01 00:00:00

  • Mycobacterial cross contamination during radiometric culturing.

    abstract::Pseudobacteremias in blood cultures performed on the BACTEC radiometric blood culture system have been reported. We report three cases of cross contamination with Mycobacterium avium that occurred when mycobacteria were cultured with the BACTEC 460 TB system. Malfunction of the needle sterilization heating block was d...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Vannier AM,Tarrand JJ,Murray PR

    更新日期:1988-09-01 00:00:00

  • Serodiagnosis of acute hepatitis B virus infection by a modified competitive binding radioimmunoassay.

    abstract::Using the Staphylococcus aureus absorption method, we have modified a commercial radioimmunoassay kit for the determination of total antibody to hepatitis B core antigen so that we can now determine the predominant globulin species of that antibody. One-hundred percent of acute hepatitis B sera had a predominance of i...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Schable CA,Maynard JE

    更新日期:1982-11-01 00:00:00

  • Restriction fragment length polymorphism typing of clinical isolates of Mycobacterium tuberculosis from patients with pulmonary tuberculosis in Madras, India, by use of direct-repeat probe.

    abstract::Large numbers of Mycobacterium tuberculosis isolates that were obtained from patients' sputa on diagnosis and during follow-up after short-course chemotherapy in Madras, India, have either no copy or only a single copy of IS6110. This poses a limitation for DNA fingerprinting with an IS6110-based probe to determine th...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Sahadevan R,Narayanan S,Paramasivan CN,Prabhakar R,Narayanan PR

    更新日期:1995-11-01 00:00:00

  • Separation of cryptosporidium oocysts from fecal debris by density gradient centrifugation and glass bead columns.

    abstract::A method was developed to obtain purified cryptosporidium oocysts from fecal samples. Oocysts were initially collected by centrifugation through a sucrose density gradient and further purified by passage through glass bead columns. The purified oocysts were antigenically active and sufficiently pure for immunological ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Heyman MB,Shigekuni LK,Ammann AJ

    更新日期:1986-04-01 00:00:00

  • Development of an enzyme-linked immunosorbent assay for caprine arthritis-encephalitis virus.

    abstract::Because relatively few caprine arthritis-encephalitis virus (CAEV)-infected animals exhibit clinical signs of illness, efforts to control and eradicate this virus will depend heavily on a sensitive diagnostic test that can be easily carried out. The currently utilized tests are of limited usefulness because of relativ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Archambault D,East N,Perk K,Dahlberg JE

    更新日期:1988-05-01 00:00:00

  • Optimal conditions for recovery of the human immunodeficiency virus from peripheral blood mononuclear cells.

    abstract::Optimal conditions for demonstrating the presence of infectious human immunodeficiency virus in peripheral blood mononuclear cells (PMCs) from seropositive individuals involved cocultivation of infected cells with phytohemagglutinin-stimulated PMCs from seronegative donors in the presence of 2 micrograms of Polybrene ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Castro BA,Weiss CD,Wiviott LD,Levy JA

    更新日期:1988-11-01 00:00:00

  • Specificity of a Mycobacterium kansasii phenolic glycolipid (mycoside A) immunoserum.

    abstract::The specificity of Mycobacterium kansasii anti-mycoside A antiserum prepared in rabbits injected with purified samples of the phenolic glycolipid was evaluated by an enzyme-linked immunosorbent assay. Chloroform-methanol extracts from representative strains of 23 mycobacterial species and 50 strains of M. kansasii sho...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Papa F,Riviere M,Fournié JJ,Puzo G,David H

    更新日期:1987-12-01 00:00:00

  • Geographical heterogeneity between Far Eastern and Western countries in prevalence of the virulence plasmid, the superantigen Yersinia pseudotuberculosis-derived mitogen, and the high-pathogenicity island among Yersinia pseudotuberculosis strains.

    abstract::Yersinia pseudotuberculosis produces novel superantigenic toxins designated YPMa (Y. pseudotuberculosis-derived mitogen), YPMb, and YPMc and has a pathogenicity island termed HPI (high-pathogenicity island) and R-HPI (the right-hand part of the HPI with truncation in its left-hand part) on the chromosome. Analysis of ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Fukushima H,Matsuda Y,Seki R,Tsubokura M,Takeda N,Shubin FN,Paik IK,Zheng XB

    更新日期:2001-10-01 00:00:00

  • Assessment of a new hub design and the semiquantitative catheter culture method using an in vivo experimental model of catheter sepsis.

    abstract::An in vivo model of hub-related catheter sepsis in rabbits is reported. The model was used to investigate the protection offered by a new hub design against external contamination by Pseudomonas aeruginosa or Staphylococcus epidermidis and to reassess the diagnostic value of the semiquantitative culture method in bact...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Segura M,Alía C,Valverde J,Franch G,Torres Rodríguez JM,Sitges-Serra A

    更新日期:1990-11-01 00:00:00

  • Diagnosis of human leptospirosis by monoclonal antibody-based antigen detection in urine.

    abstract::Hybridomas secreting specific monoclonal antibodies (MAb) to all members of the genus Leptospira (clone LF9) and those that are specific only to the pathogenic species (clones LD5 and LE1) were produced. MAb LF9, which was immunoglobulin G1 (IgG1), reacted to a 38-kDa component of the sodium dodecyl sulfate-polyacryla...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Saengjaruk P,Chaicumpa W,Watt G,Bunyaraksyotin G,Wuthiekanun V,Tapchaisri P,Sittinont C,Panaphut T,Tomanakan K,Sakolvaree Y,Chongsa-Nguan M,Mahakunkijcharoen Y,Kalambaheti T,Naigowit P,Wambangco MA,Kurazono H,Hayashi H

    更新日期:2002-02-01 00:00:00

  • Assessment of Etest as an alternative to agar dilution for antimicrobial susceptibility testing of Neisseria gonorrhoeae.

    abstract::We studied whether the Etest can be used as an alternative to agar dilution to determine antimicrobial susceptibilities of ceftriaxone, cefixime, and cefpodoxime in Neisseria gonorrhoeae surveillance. One hundred fifteen clinical and laboratory isolates of N. gonorrhoeae were tested following the Clinical Laboratory I...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Liu H,Taylor TH Jr,Pettus K,Trees D

    更新日期:2014-05-01 00:00:00