Quantitative competitive PCR with bronchoalveolar lavage fluid for diagnosis of toxoplasmosis in AIDS patients.


:Of 121 AIDS patients, 12 (10%) had Toxoplasma gondii DNA detected by competitive PCR in their bronchoalveolar lavage samples. Quantitation of the PCR results showed a correlation between the parasite burden and the serum lactic dehydrogenase titer. Quantitative competitive PCR could be useful to assess the diagnosis and the severity of pulmonary toxoplasmosis.


J Clin Microbiol


Bretagne S,Costa JM,Fleury-Feith J,Poron F,Dubreuil-Lemaire ML,Vidaud M




Has Abstract


1995-06-01 00:00:00












  • Broad-Range PCR Coupled with Electrospray Ionization Time of Flight Mass Spectrometry for Detection of Bacteremia and Fungemia in Patients with Neutropenic Fever.

    abstract::Infection is an important complication in patients with hematologic malignancies or solid tumors undergoing intensive cytotoxic chemotherapy. In only 20 to 30% of the febrile neutropenic episodes, an infectious agent is detected by conventional cultures. In this prospective study, the performance of broad-range PCR co...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Desmet S,Maertens J,Bueselinck K,Lagrou K

    更新日期:2016-10-01 00:00:00

  • Liquid medium for growth of Legionella pneumophila.

    abstract::The medium described is a simple yeast extract broth capable of growing large number of Legionella neumophila, the causative organism of Legionnaires disease. Filtration was chosen as a means of sterilization, since medium that was autoclaved did not support growth without the presence of Norite A. The filtered medium...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Ristroph JD,Hedlund KW,Allen RG

    更新日期:1980-01-01 00:00:00

  • Multicenter evaluation of a sequence-based protocol for subtyping Shiga toxins and standardizing Stx nomenclature.

    abstract::When Shiga toxin-producing Escherichia coli (STEC) strains emerged as agents of human disease, two types of toxin were identified: Shiga toxin type 1 (Stx1) (almost identical to Shiga toxin produced by Shigella dysenteriae type 1) and the immunologically distinct type 2 (Stx2). Subsequently, numerous STEC strains have...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究


    authors: Scheutz F,Teel LD,Beutin L,Piérard D,Buvens G,Karch H,Mellmann A,Caprioli A,Tozzoli R,Morabito S,Strockbine NA,Melton-Celsa AR,Sanchez M,Persson S,O'Brien AD

    更新日期:2012-09-01 00:00:00

  • Phage heterogeneity of coagulase-negative staphylococci isolated in the United States and Sweden from bovine milk.

    abstract::A Swedish bovine and a Dutch human phage set for coagulase-negative staphylococci were used to phage type coagulase-negative staphylococci isolated from bovine milk from Minnesota dairy herds. A comparison was also made of the deoxyribonuclease activity of coagulase-negative staphylococci isolated from bovine milk in ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Holmberg O,Lyon RH,Price D,Farnsworth RJ

    更新日期:1979-09-01 00:00:00

  • Nuclease enhancement of specific cell agglutination in a serodiagnostic test for Neisseria gonorrhoeae.

    abstract::Antiserum to a purified type R lipopolysaccharide antigen isolated from Neisseria gonorrhoeae was used in a slide agglutination test and compared with conventional carbohydrate utilization and fluorescent antibody tests to confirm the identity of laboratory cultures classified as typical or "atypical" N. gonorrhoeae. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Arko RJ,Wong KH,Peacock WL

    更新日期:1979-04-01 00:00:00

  • Evaluation of MicroScan rapid gram-positive panels for detection of oxacillin-resistant staphylococci.

    abstract::The ability of MicroScan rapid panels (Baxter MicroScan, West Sacramento, Calif.) to detect oxacillin resistance in 92 clinical isolates of Staphylococcus aureus and 103 coagulase-negative staphylococci was evaluated by comparing results with those of MicroScan 24-h MIC panels and, to resolve discrepancies, oxacillin ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Woods GL,LaTemple D,Cruz C

    更新日期:1994-04-01 00:00:00

  • Evaluation of phenotypic markers for selection and identification of Candida dubliniensis.

    abstract::Candida dubliniensis is often associated with C. albicans in cultures. Easy-to-perform selective isolation procedures for these closely related species do not exist. Therefore, we evaluated previously described discriminatory phenotypic markers for C. dubliniensis. A total of 150 oral rinses from human immunodeficienc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Tintelnot K,Haase G,Seibold M,Bergmann F,Staemmler M,Franz T,Naumann D

    更新日期:2000-04-01 00:00:00

  • Comparison of Gen-Probe DNA probe test and culture for the detection of Neisseria gonorrhoeae in endocervical specimens.

    abstract::A 2-h nonisotopic DNA probe assay for the direct detection of Neisseria gonorrhoeae in urogenital specimens has recently been modified (PACE 2; Gen-Probe, San Diego, Calif.). The new assay format was developed to increase the sensitivity of the assay and simplify procedural steps. In this study, the new DNA probe test...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Panke ES,Yang LI,Leist PA,Magevney P,Fry RJ,Lee RF

    更新日期:1991-05-01 00:00:00

  • Development of two antibody detection enzyme-linked immunosorbent assays for serodiagnosis of human chronic fascioliasis.

    abstract::Coprological examination based on egg detection in stool samples is currently used as the gold standard for the diagnosis of human fascioliasis. However, this method is not effective during the acute phase of the disease and has poor sensitivity during the chronic phase. Serodiagnosis has become an excellent alternati...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Cabán-Hernández K,Gaudier JF,Ruiz-Jiménez C,Espino AM

    更新日期:2014-03-01 00:00:00

  • Diagnosis of placental malaria.

    abstract::In a group of 596 delivering Ghanaian women, the sensitivities of peripheral blood thick film microscopy, ICT Malaria P.f/P.v test, and PCR in detecting microscopically confirmed placental Plasmodium falciparum infection were 42, 80, and 97%, respectively. In addition to the gross underestimation of placental malaria ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Mockenhaupt FP,Ulmen U,von Gaertner C,Bedu-Addo G,Bienzle U

    更新日期:2002-01-01 00:00:00

  • Luminol-amplified chemiluminescence: a sensitive method for detecting the carrier state in chronic granulomatous disease.

    abstract::Patients with chronic granulomatous disease have a marked defect in neutrophil oxidative metabolism and microbicidal activity. Asymptomatic mothers of males with the disease can usually be identified as heterozygous carriers by intermediate leukocyte function. Most mothers of females with the disease, however, have no...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Mills EL,Rholl KS,Quie PG

    更新日期:1980-07-01 00:00:00

  • Evaluation of a rapid immunochromatographic test for detection of antibodies to human immunodeficiency virus.

    abstract::A new immunochromatographic rapid test, Determine HIV-1/2, for the detection of antibodies to human immunodeficiency virus type 1 (HIV-1) and HIV-2 in human whole blood, serum, and plasma was evaluated. Determine HIV-1/2 is a sandwich immunoassay and uses a nitrocellulose strip with a capture site for the patient's re...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Arai H,Petchclai B,Khupulsup K,Kurimura T,Takeda K

    更新日期:1999-02-01 00:00:00

  • Evaluation of Streck tissue fixative, a nonformalin fixative for preservation of stool samples and subsequent parasitologic examination.

    abstract::We undertook a study to evaluate Streck tissue fixative (STF) as a substitute for formalin and polyvinyl alcohol (PVA) in fecal preservation. A comparison of formalin, PVA, (mercuric chloride based), and STF was done by aliquoting fecal samples into each fixative. Stool specimens were collected in Haiti, and parasites...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Nace EK,Steurer FJ,Eberhard ML

    更新日期:1999-12-01 00:00:00

  • Evidence for indirect nosocomial transmission of Neisseria meningitidis resulting in two cases of invasive meningococcal disease.

    abstract::Nosocomial transmission of Neisseria meningitidis has only rarely been reported. Here, we present a significant spatiotemporal association of two cases of invasive meningococcal disease identified by retrospective cluster analysis with the program SaTScan. The most likely epidemiological link was simultaneous hospital...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Elias J,Health Office in the Rural District Office Wartburgkreis.,Claus H,Frosch M,Vogel U

    更新日期:2006-11-01 00:00:00

  • Distribution and molecular characterization of Porphyromonas gingivalis carrying a new type of fimA gene.

    abstract::Fimbriae of Porphyromonas gingivalis are filamentous appendages on the cell surface and are thought to be one of the virulence factors. The fimA gene encoding the subunit protein of fimbriae, fimbrillin (FimA), was classified into four typeable variants (types I to IV). We previously examined the distribution of P. gi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Nakagawa I,Amano A,Kimura RK,Nakamura T,Kawabata S,Hamada S

    更新日期:2000-05-01 00:00:00

  • Comparison between Saliva and Nasopharyngeal Swab Specimens for Detection of Respiratory Viruses by Multiplex Reverse Transcription-PCR.

    abstract::Nasopharyngeal swabs (NPSs) are being widely used as specimens for multiplex real-time reverse transcription (RT)-PCR for respiratory virus detection. However, it remains unclear whether NPS specimens are optimal for all viruses targeted by multiplex RT-PCR. In addition, the procedure to obtain NPS specimens causes co...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kim YG,Yun SG,Kim MY,Park K,Cho CH,Yoon SY,Nam MH,Lee CK,Cho YJ,Lim CS

    更新日期:2016-12-28 00:00:00

  • PCR as a confirmatory technique for laboratory diagnosis of malaria.

    abstract::We compared a nested PCR assay and microscopic examination of Giemsa-stained blood films for detection and identification of Plasmodium spp. in blood specimens. PCR was more sensitive than microscopy and capable of identifying malaria parasites at the species level when microscopy was equivocal. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Johnston SP,Pieniazek NJ,Xayavong MV,Slemenda SB,Wilkins PP,da Silva AJ

    更新日期:2006-03-01 00:00:00

  • International surveillance of bloodstream infections due to Candida species: frequency of occurrence and antifungal susceptibilities of isolates collected in 1997 in the United States, Canada, and South America for the SENTRY Program. The SENTRY Participa

    abstract::An international program of surveillance of bloodstream infections (BSIs) in the United States, Canada, and South America between January and December 1997 detected 306 episodes of candidemia in 34 medical centers (22 in the United States, 6 in Canada, and 6 in South America). Eighty percent of the BSIs were nosocomia...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Pfaller MA,Jones RN,Doern GV,Sader HS,Hollis RJ,Messer SA

    更新日期:1998-07-01 00:00:00

  • Real-time cellular analysis coupled with a specimen enrichment accurately detects and quantifies Clostridium difficile toxins in stool.

    abstract::We describe here the use of an immunomagnetic separation enrichment process coupled with a modified real-time cellular analysis (RTCA) system (RTCA version 2) for the detection of C. difficile toxin (CDT) in stool. The limit of CDT detection by RTCA version 2 was 0.12 ng/ml. Among the consecutively collected 401 diarr...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Huang B,Jin D,Zhang J,Sun JY,Wang X,Stiles J,Xu X,Kamboj M,Babady NE,Tang YW

    更新日期:2014-04-01 00:00:00

  • Clinical trial comparing bacitracin with Strep-A-Chek for accuracy and turnaround time in the presumptive identification of Streptococcus pyogenes.

    abstract::In a clinical trial, Strep-A-Chek (a 10-min chromogenic test) was compared with the bacitracin disk susceptibility test for accuracy and turnaround time in the presumptive identification of Streptococcus pyogenes. Among 461 isolates of beta-hemolytic streptococci (344 throat isolates and 117 isolates from other sites)...

    journal_title:Journal of clinical microbiology

    pub_type: 临床试验,杂志文章


    authors: Yajko DM,Lawrence J,Nassos P,Young J,Hadley WK

    更新日期:1986-09-01 00:00:00

  • Replication of murine paramyxoviruses in hamster tracheal organ culture and comparison with standard tissue culture methods.

    abstract::Replication of Sendai virus, pneumonia virus of mice, and SV5 was investigated in tracheal organ cultures from 2- to 4-day-old and 2- and 4-week-old hamsters, and viral infectivity in tracheal explants was compared with that in tissue culture monolayers. Explants from 2- to 4-day-old hamsters produced higher titers of...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Schiff LJ

    更新日期:1976-09-01 00:00:00

  • Name Changes for Fungi of Medical Importance, 2018 to 2019.

    abstract::The current article summarizes recent changes in nomenclature for fungi of medical importance published in the years 2018 to 2019, including new species and revised names for existing ones. Many of the revised names have been widely adopted without further discussion. However, those that concern common pathogens of hu...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,评审


    authors: Borman AM,Johnson EM

    更新日期:2021-01-21 00:00:00

  • A Panton-Valentine leucocidin (PVL)-positive community-acquired methicillin-resistant Staphylococcus aureus (MRSA) strain, another such strain carrying a multiple-drug resistance plasmid, and other more-typical PVL-negative MRSA strains found in Japan.

    abstract::Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) was collected from children with bullous impetigo in 2003 and 2004. One strain collected in 2003 was Panton-Valentine leucocidin (PVL) positive. In 2004, a multiple-drug-resistant PVL(+) CA-MRSA strain was isolated from an athlete with a cutaneou...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Takizawa Y,Taneike I,Nakagawa S,Oishi T,Nitahara Y,Iwakura N,Ozaki K,Takano M,Nakayama T,Yamamoto T

    更新日期:2005-07-01 00:00:00

  • Expression of type 8 capsular polysaccharide and production of toxic shock syndrome toxin 1 are associated among vaginal isolates of Staphylococcus aureus.

    abstract::A colony immunoblot method was developed for serotyping the capsular polysaccharides expressed by Staphylococcus aureus isolates. The method was rapid and specific and was performed with either polyclonal or monoclonal antibodies specific for each of the capsule types. S. aureus isolates were obtained from patients wi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lee JC,Liu MJ,Parsonnet J,Arbeit RD

    更新日期:1990-12-01 00:00:00

  • Molecular parameters for precise diagnosis of asymptomatic Epstein-Barr virus reactivation in healthy carriers.

    abstract::Asymptomatic Epstein-Barr virus (EBV) reactivations periodically occur in oral mucosa-associated lymphoid tissues. Until now, EBV reactivation has been diagnosed by serologic profiles that suggest virus replication. Serologic responses, however, are delayed and do not necessarily indicate ongoing replicative activity....

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Maurmann S,Fricke L,Wagner HJ,Schlenke P,Hennig H,Steinhoff J,Jabs WJ

    更新日期:2003-12-01 00:00:00

  • Corynebacterium resistens sp. nov., a new multidrug-resistant coryneform bacterium isolated from human infections.

    abstract::Five strains of an unknown, multidrug-resistant coryneform, gram-positive rod were isolated from blood, bronchial aspirate, and abscess specimens. Four of the five strains isolated were highly resistant to antimicrobial agents, including beta-lactams, aminoglycosides, macrolides, quinolones, and tetracyclines, except ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Otsuka Y,Kawamura Y,Koyama T,Iihara H,Ohkusu K,Ezaki T

    更新日期:2005-08-01 00:00:00

  • Use of subtractive hybridization to identify a diagnostic probe for a cystic fibrosis epidemic strain of Pseudomonas aeruginosa.

    abstract::A multiresistant strain of Pseudomonas aeruginosa is widespread among cystic fibrosis (CF) patients attending clinics in Liverpool, United Kingdom. Suppression subtractive hybridization was used to identify sequences present in the Liverpool CF epidemic strain but absent from strain PAO1. Using dot blot and PCR amplif...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Parsons YN,Panagea S,Smart CH,Walshaw MJ,Hart CA,Winstanley C

    更新日期:2002-12-01 00:00:00

  • Use of four dengue virus antigens for determination of dengue immune status by enzyme-linked immunosorbent assay of immunoglobulin G avidity.

    abstract::We used an enzyme-linked immunosorbent assay (ELISA) of immunoglobulin G avidity to determine the dengue immune status of 105 pairs of serum samples from patients infected with dengue virus. This study shows that a simple avidity test, for which only one acute-phase serum sample is required, is potentially more useful...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Matheus S,Deparis X,Labeau B,Lelarge J,Morvan J,Dussart P

    更新日期:2005-11-01 00:00:00

  • Monoclonal antibodies to Pasteurella haemolytica serotype 1 lipopolysaccharide: demonstration of antigenic similarities among several serotypes.

    abstract::Murine monoclonal antibodies (MAbs) were produced which were specific for Pasteurella haemolytica serotype 1 lipopolysaccharide (LPS). The MAbs also reacted with LPS present in a partially purified antigen derived from a saline extract of the organism. The epitope to which the MAbs were directed was a carbohydrate whi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Durham JA,Antone SM,Cunningham MW,Confer AW

    更新日期:1988-05-01 00:00:00

  • Direct detection and characterization of Shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA, and saa.

    abstract::We recently described a novel megaplasmid-encoded adhesin produced by certain Shiga toxigenic Escherichia coli (STEC) strains that lack the locus for enterocyte effacement (LEE) pathogenicity island. This adhesin, designated Saa (STEC autoagglutinating adhesin), may be a marker for a subset of LEE-negative STEC strain...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Paton AW,Paton JC

    更新日期:2002-01-01 00:00:00