Evaluation of commercial enzyme-linked immunosorbent assay kits for detection of tuberculosis in Argentinean population.

abstract：:Oligonucleotide chips targeting the bacterial internal transcribed spacer region (ITS) of the 16S-23S rRNA gene, which contains genus- and species-specific regions, were developed and evaluated. Forty-three sequences were designed consisting of 1 universal, 3 Gram stain-specific, 9 genus-specific, and 30 species-speci...

journal_title：Journal of clinical microbiology

authors： Kim CM,Song ES,Jang HJ,Kim HJ,Lee S,Shin JH,Kim SJ,Jeong SH,Jeong J,Koh K,Choi GE,Lee EY,Chang CL

更新日期：2010-05-01 00:00:00

abstract：:E test strips (AB Biodisk, Culver City, Calif.) tested on 2% NaCl-supplemented Mueller-Hinton agar and Alamar panels (Alamar, Sacramento, Calif.) correctly characterized 127 of 127 methicillin-resistant Staphylococcus aureus isolates and 100 of 100 methicillin-susceptible S. aureus isolates. These overnight antimicrob...

journal_title：Journal of clinical microbiology

authors： Novak SM,Hindler J,Bruckner DA

更新日期：1993-11-01 00:00:00

abstract：:Antiretroviral therapy (ART) during acute HIV infection (AHI) interrupts viral dynamics and may delay the emergence of serological markers targeted by current HIV screening and confirmatory assays, thus creating challenges for correctly classifying HIV infection status. The performance of three HIV antigen/antibody co...

journal_title：Journal of clinical microbiology

authors： Manak MM,Jagodzinski LL,Shutt A,Malia JA,Leos M,Ouellette J,Akapirat S,Colby DL,Phanuphak N,Eller LA,Robb ML,de Souza M,Ananworanich J,Peel SA,RV254\/SEARCH010 and the RV217 Study Teams.

更新日期：2019-09-24 00:00:00

abstract：:Two ambient-temperature, dry plasma transport systems, ViveST tubes and RNASound RNA sampling cards, and two extraction methods were compared to frozen plasma for HIV-1 RNA recovery. Significant RNA loss occurred: ViveST+MiniMag > ViveST+QIAamp > RNASound+QIAamp. RNA loss and low specimen volumes may affect the sensit...

journal_title：Journal of clinical microbiology

authors： Levine M,Beck I,Styrchak S,Pepper G,Frenkel L

更新日期：2016-07-01 00:00:00

abstract：:Molecular analysis of Mycobacterium ulcerans has revealed two new insertion sequences (ISs), IS2404 and IS2606. IS2404 was identified by complete sequencing of a previously described repetitive DNA segment from M. ulcerans. This element is 1,274 bp long, contains 12-bp inverted repeats and a single open reading frame ...

journal_title：Journal of clinical microbiology

authors： Stinear T,Ross BC,Davies JK,Marino L,Robins-Browne RM,Oppedisano F,Sievers A,Johnson PD

更新日期：1999-04-01 00:00:00

abstract：:A space-saving media table designed to facilitate pouring large quantities of plated media in a small area is described. ...

journal_title：Journal of clinical microbiology

authors： McGonagle LA,Schmidt G,Eng S,Wirsing K

更新日期：1975-07-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) resistance to antivirals is a major problem in immunocompromised patients. Drug resistance is characterized by phenotypic testing or genotypic analysis of the phosphotransferase (UL97) and DNA polymerase (UL54) genes. However, genotypic assays require further characterization of unknown mu...

journal_title：Journal of clinical microbiology

authors： Drouot E,Piret J,Boivin G

更新日期：2013-10-01 00:00:00

abstract：:We studied the evolution of Vibrio cholerae O1 during the 1991 to 2000 cholera epidemic in Mexico by biochemical, serological, and molecular characterization of strains collected during this period. Strains were divided into toxigenic and nontoxigenic groups according to the presence or absence of genes encoding chole...

journal_title：Journal of clinical microbiology

authors： Lizárraga-Partida ML,Quilici ML

更新日期：2009-05-01 00:00:00

abstract：:A rapidly fatal case of pulmonary tularemia in a 43-year-old man who was transferred to a tertiary care facility is presented. The microbiology laboratory and autopsy services were not notified of the clinical suspicion of tularemia by the service caring for the patient. Despite having a laboratory bioterrorism proced...

journal_title：Journal of clinical microbiology

authors： Shapiro DS,Schwartz DR

更新日期：2002-06-01 00:00:00

abstract：:Pseudomonas gladioli was isolated from 11 patients with cystic fibrosis. It resembled Pseudomonas cepacia on the selective and differential medium OFPBL, producing yellow colonies after 48 to 72 h of incubation. Isolates were characterized biochemically, by DNA hybridization, and by cellular fatty acid analysis. A rev...

journal_title：Journal of clinical microbiology

authors： Christenson JC,Welch DF,Mukwaya G,Muszynski MJ,Weaver RE,Brenner DJ

更新日期：1989-02-01 00:00:00

abstract：:Humoral immune response to intestinal Rhodococcus (Corynebacterium) equi in horses was studied by enzyme-linked immunosorbent assay. Anti-R. equi immunoglobulin M (IgM), IgG, and IgA antibodies were demonstrated in the healthy horse population. Adult horse levels of anti-R. equi IgM and IgG antibodies were reached by ...

journal_title：Journal of clinical microbiology

authors： Takai S,Kawazu S,Tsubaki S

更新日期：1986-05-01 00:00:00

abstract：:The highly specific and sensitive latex agglutination test for cryptococcal antigen detection in cerebrospinal fluid is routine in many hospitals. Contamination of cerebrospinal fluid by a minute amount of syneresis fluid (surface condensation) from agar gave a strongly positive reaction which was heat stable, was not...

journal_title：Journal of clinical microbiology

authors： Boom WH,Piper DJ,Ruoff KL,Ferraro MJ

更新日期：1985-11-01 00:00:00

abstract：:We studied 315 coagulase-negative Staphylococcus strains recovered prospectively during 240 surgical procedures (206 subjects) from proven or suspected device-associated bone and joint infections. Sixteen strains (5.1%) had decreased susceptibility to glycopeptides: 15 (12 S. epidermidis strains, 2 S. capitis strains,...

journal_title：Journal of clinical microbiology

authors： Cremniter J,Slassi A,Quincampoix JC,Sivadon-Tardy V,Bauer T,Porcher R,Lortat-Jacob A,Piriou P,Judet T,Herrmann JL,Gaillard JL,Rottman M

更新日期：2010-04-01 00:00:00

abstract：:Parasite-specific humoral and cellular immune responses were evaluated in in nine immunosuppressed patients with Strongyloides stercoralis infection. Four patients had the disseminated form of the disease, and five had serious manifestations without evidence of hyperinfection. All patients had high levels of immunoglo...

journal_title：Journal of clinical microbiology

authors： Genta RM,Douce RW,Walzer PD

更新日期：1986-06-01 00:00:00

abstract：:Carbapenem-resistant Enterobacter species are emerging nosocomial pathogens. As with most multidrug-resistant Gram-negative pathogens, the polymyxins are often the only therapeutic option. In this study involving clinical isolates of E. cloacae and E. aerogenes, susceptibility testing methods with polymyxin B were ana...

journal_title：Journal of clinical microbiology

authors： Landman D,Salamera J,Quale J

更新日期：2013-12-01 00:00:00

abstract：:Single colonies of beta-hemolytic streptococci could be grouped by antibody-coated latex bead agglutination or coagglutination with staphylococci coated with antibody after incubation of the colonies with mutanolysin. This simple and quick procedure provided an enzymatic means of enhancing the sensitivity of tests suc...

journal_title：Journal of clinical microbiology

authors： Calandra GB,Henson CL

更新日期：1982-01-01 00:00:00

abstract：:Sequence detection by the 5' nuclease TaqMan assay uses online detection of internal fluorogenic probes in closed PCR tubes. Primers and probe were chosen from a part of the omlA gene common to all serotypes of Actinobacillus pleuropneumoniae, which gave an amplicon of 92 bp. The test was evaluated with 73 lung isolat...

journal_title：Journal of clinical microbiology

authors： Angen O,Jensen J,Lavritsen DT

更新日期：2001-01-01 00:00:00

abstract：:Genetic determinants of the invasive phenotype of Shigella spp. and enteroinvasive Escherichia coli (EIEC), two common agents of bacillary dysentery, are encoded on large (180- to 210 kilobase), nonconjugative plasmids. Several plasmid-encoded antigens have been implicated as important bacterial ligands that mediate t...

journal_title：Journal of clinical microbiology

authors： Venkatesan M,Buysse JM,Vandendries E,Kopecko DJ

更新日期：1988-02-01 00:00:00

abstract：:In 1998, an outbreak of systemic infections caused by Bacillus cereus occurred in the Neonatal Intensive Care Unit of the University Hospital Vrije Universiteit, Amsterdam, The Netherlands. Three neonates developed sepsis with positive blood cultures. One neonate died, and the other two neonates recovered. An environm...

journal_title：Journal of clinical microbiology

authors： Van Der Zwet WC,Parlevliet GA,Savelkoul PH,Stoof J,Kaiser AM,Van Furth AM,Vandenbroucke-Grauls CM

更新日期：2000-11-01 00:00:00

abstract：:Bacterial vaginosis (BV) is the most common cause of vaginal discharge in reproductive-age women. BV has been associated with poor reproductive outcomes such as preterm delivery, the acquisition of sexually transmitted infections, including HIV, and pelvic inflammatory disease. BV represents the acquisition of a diver...

journal_title：Journal of clinical microbiology

authors： Coleman JS,Gaydos CA

更新日期：2018-08-27 00:00:00

abstract：:An enzyme-linked immunosorbent assay method was used to classify bovine serum as positive, negative, or doubtful for antibodies to Brucella abortus. Spectrophotometric data from assays of 64 serologically positive and 32 serologically negative bovine sera were analyzed statistically to define the range of spectrophoto...

journal_title：Journal of clinical microbiology

authors： Heck FC,Williams JD,Pruett J

更新日期：1980-04-01 00:00:00

abstract：:Three hundred sixty Enterobacteriaceae and nonfermenting gram-negative bacilli, isolated during one week in May 2004 at five hospitals in Amsterdam, The Netherlands, were evaluated for the presence of extended-spectrum beta-lactamases (ESBLs). A prevalence of 7.8% was found, in contrast to the 1% observed in 1997. CTX...

journal_title：Journal of clinical microbiology

authors： al Naiemi N,Bart A,de Jong MD,Vandenbroucke-Grauls CM,Rietra PJ,Debets-Ossenkopp YJ,Wever PC,Spanjaard L,Bos AJ,Duim B

更新日期：2006-08-01 00:00:00

abstract：:Recent evidence supports a causal relationship between Bartonella (Rochalimaea) henselae, cat-scratch disease (CSD), and bacillary angiomatosis. Cats appear to be the primary reservoir. Blood from 19 cats owned by 14 patients diagnosed with CSD was cultured. Blood samples from cats owned by veterinary students (n = 25...

journal_title：Journal of clinical microbiology

authors： Kordick DL,Wilson KH,Sexton DJ,Hadfield TL,Berkhoff HA,Breitschwerdt EB

更新日期：1995-12-01 00:00:00

abstract：:To select a method for detecting methicillin-resistant Staphylococcus aureus (MRSA) in nasal swabs, we compared BD GeneOhm MRSA PCR and various culture media (mannitol salt agar with cefoxitin, MRSASelect, CHROMagar MRSA, and Spectra MRSA). While PCR detection of MRSA was more rapid, MRSASelect and Spectra MRSA demons...

journal_title：Journal of clinical microbiology

authors： Bischof LJ,Lapsley L,Fontecchio K,Jacosalem D,Young C,Hankerd R,Newton DW

更新日期：2009-07-01 00:00:00

abstract：:Two strains of a previously undescribed Actinomyces-like bacterium were recovered in pure culture from infected root canals of teeth. Analysis by biochemical testing and polyacrylamide gel electrophoresis of whole-cell proteins indicated that the strains closely resembled each other phenotypically but were distinct fr...

journal_title：Journal of clinical microbiology

authors： Collins MD,Hoyles L,Kalfas S,Sundquist G,Monsen T,Nikolaitchouk N,Falsen E

更新日期：2000-09-01 00:00:00

abstract：:Comparative studies on the detection of bovine serum immunoglobulin G antibodies to bluetongue virus with an enzyme-linked immunosorbent assay, an immunodiffusion method, and a serum neutralization assay demonstrated complete concordance between the enzyme-linked immunosorbent assay and the serum neutralization assay ...

journal_title：Journal of clinical microbiology

authors： Poli G,Stott J,Liu YS,Manning JS

更新日期：1982-01-01 00:00:00

abstract：:Studies of human immunodeficiency virus type 1 (HIV-1) transmission suggest that genital HIV-1 RNA and DNA may both be determinants of HIV-1 infectivity. Despite its potential role in HIV-1 transmission, there are limited quantitative data on genital HIV-1 DNA. Here we validated an in-house real-time PCR method for qu...

journal_title：Journal of clinical microbiology

authors： Benki S,McClelland RS,Emery S,Baeten JM,Richardson BA,Lavreys L,Mandaliya K,Overbaugh J

更新日期：2006-12-01 00:00:00

abstract：:Morganella ("Proteus") morganii is the only species in the recently proposed genus Morganella, but we suspect there are yet undescribed species in the genus. Two candidates for new species were recently investigated. Nineteen strains isolated from clinical specimens resembled M. morganii but were lysine positive and n...

journal_title：Journal of clinical microbiology

authors： Hickman FW,Framer JJ 3rd,Steigerwalt AG,Brenner DJ

更新日期：1980-07-01 00:00:00

abstract：:We previously reported on the efficacy of the enzyme-linked immunoglobulin M capture immune complex (IC) biotinylated antigen assay (EMIBA) for the seroconfirmation of early Lyme disease and active infection with Borrelia burgdorferi. In earlier work we identified non-cross-reacting epitopes of a number of B. burgdorf...

journal_title：Journal of clinical microbiology

authors： Brunner M,Stein S,Mitchell PD,Sigal LH

更新日期：1998-04-01 00:00:00

abstract：:The present investigation focused on genetic diversity and drug resistance of 101 Mycobacterium tuberculosis strains isolated between July 2003 and February 2005 in the Okinawa prefecture, Ryukyu Islands, Japan. A high rate of clustering (87%, eight clusters, 2 to 69 strains/cluster) was observed upon spoligotyping; m...

journal_title：Journal of clinical microbiology

authors： Millet J,Miyagi-Shiohira C,Yamane N,Sola C,Rastogi N

更新日期：2007-11-01 00:00:00