Exposure of laboratory workers to Francisella tularensis despite a bioterrorism procedure.

abstract：:A toxic shock syndrome toxin 1 (TSST-1) antibody-binding protein produced by an ovine-associated strain of Staphylococcus aureus was examined. The protein showed total identity to TSST-1 by immunodiffusion analysis. Western blots (immunoblots) of proteins separated by isoelectric focusing revealed that the TSST-1 anti...

journal_title：Journal of clinical microbiology

authors： Ho G,Campbell WH,Carlson E

更新日期：1989-01-01 00:00:00

abstract：:Use of whole-genome sequencing (WGS) for routine mycobacterial species identification and drug susceptibility testing (DST) is becoming a reality. We compared the performances of WGS and standard laboratory workflows prospectively, by parallel processing at a major mycobacterial reference service over the course of 1 ...

journal_title：Journal of clinical microbiology

authors： Quan TP,Bawa Z,Foster D,Walker T,Del Ojo Elias C,Rathod P,MMM Informatics Group.,Iqbal Z,Bradley P,Mowbray J,Walker AS,Crook DW,Wyllie DH,Peto TEA,Smith EG

更新日期：2018-01-24 00:00:00

abstract：:An outbreak of coronavirus disease 2019 (COVID-19) caused by a novel coronavirus (severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2]) began in Wuhan, Hubei, China, in December 2019 and spread rapidly worldwide. The response by the Alberta Precision Laboratories, Public Health Laboratory (ProvLab), AB, Canada...

journal_title：Journal of clinical microbiology

authors： Pabbaraju K,Wong AA,Douesnard M,Ma R,Gill K,Dieu P,Fonseca K,Zelyas N,Tipples GA

更新日期：2020-07-23 00:00:00

abstract：:A simple enzyme-linked immunosorbent assay was developed for the identification of cultured mycobacteria belonging to the Mycobacterium tuberculosis complex, the Mycobacterium avium complex, and Mycobacterium kansasii. Six monoclonal antibodies were used: two (F23-49 and F24-2) were specific for the M. tuberculosis co...

journal_title：Journal of clinical microbiology

authors： Schöningh R,Verstijnen CP,Kuijper S,Kolk AH

更新日期：1990-04-01 00:00:00

abstract：:Naturally acquired murine cytomegalovirus (MCMV) infection in laboratory strains of mice induces antibody levels which are generally undetectable by standard techniques; therefore, MCMV has not been included routinely in mouse viral antibody screening programs. The relative sensitivity of three assay systems, the nucl...

journal_title：Journal of clinical microbiology

authors： Anderson CA,Murphy JC,Fox JG

更新日期：1983-10-01 00:00:00

abstract：:Human milk obtained from 21 American nursing mothers was studied for the presence of secretory immunoglobulin A antibody to rotavirus, the most common etiological agent of infantile gastroenteritis. Antibody was quantitated by adaptation of a recently described solid-phase radioimmunoassay technique that employs simia...

journal_title：Journal of clinical microbiology

authors： Cukor G,Blacklow NR,Capozza FE,Panjvani ZF,Bednarek F

更新日期：1979-01-01 00:00:00

abstract：:The performances of the Etest and the disk diffusion methods for testing of the susceptibilities of 235 Candida glabrata isolates to fluconazole and voriconazole were compared with that of the National Committee for Clinical Laboratory Standards (NCCLS) approved standard broth microdilution (BMD) method. The NCCLS met...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Diekema DJ,Boyken L,Messer SA,Tendolkar S,Hollis RJ

更新日期：2003-05-01 00:00:00

abstract：:Beagle dogs inoculated intravenously with 1.75 x 10(9) viable Encephalitozoon cuniculi spores at 12.5 months of age were monitored for 18 months to assess infection in the adult dog. Parameters monitored included packed cell volume, total and differential leukocyte counts, and humoral and cellular responses to infecti...

journal_title：Journal of clinical microbiology

authors： Szabo JR,Shadduck JA

更新日期：1988-03-01 00:00:00

abstract：:Pluronic polyols markedly improved the growth of two human lymphocyte cell lines when added to the growth medium in concentrations of 0.05 to 0.1%. The results of the current studies suggest that, in addition to the protective effect of polyols against mechanical damage of mammalian cells in submerged cultures, the pl...

journal_title：Journal of clinical microbiology

authors： Mizrahi A

更新日期：1975-07-01 00:00:00

abstract：:Screening for HIV infection has improved since the first immunoassays. Today, diagnosis of HIV infection can be performed with fourth-generation tests that track both the patient's antibodies and HIV antigen. The objective of this study was to evaluate the clinical sensitivity and specificity of the new DiaSorin Liais...

journal_title：Journal of clinical microbiology

authors： Lemee V,Leoz M,Etienne M,De Oliveira F,Plantier JC

更新日期：2014-09-01 00:00:00

abstract：:Nasopharyngeal swabs (NPSs) are being widely used as specimens for multiplex real-time reverse transcription (RT)-PCR for respiratory virus detection. However, it remains unclear whether NPS specimens are optimal for all viruses targeted by multiplex RT-PCR. In addition, the procedure to obtain NPS specimens causes co...

journal_title：Journal of clinical microbiology

authors： Kim YG,Yun SG,Kim MY,Park K,Cho CH,Yoon SY,Nam MH,Lee CK,Cho YJ,Lim CS

更新日期：2016-12-28 00:00:00

abstract：:Between 1 and 2% of the population in the developed world experiences a nonhealing or chronic wound characterized by an apparent arrest in a stage dominated by inflammatory processes. Lately, research groups have proposed that bacteria might be involved in and contribute to the lack of healing of these wounds. To inve...

journal_title：Journal of clinical microbiology

authors： Kirketerp-Møller K,Jensen PØ,Fazli M,Madsen KG,Pedersen J,Moser C,Tolker-Nielsen T,Høiby N,Givskov M,Bjarnsholt T

更新日期：2008-08-01 00:00:00

abstract：:Sequence-based typing (SBT), analogous to multilocus sequence typing (MLST), is the current "gold standard" typing method for investigation of legionellosis outbreaks caused by Legionella pneumophila However, as common sequence types (STs) cause many infections, some investigations remain unresolved. In this study, va...

journal_title：Journal of clinical microbiology

authors： David S,Mentasti M,Tewolde R,Aslett M,Harris SR,Afshar B,Underwood A,Fry NK,Parkhill J,Harrison TG

更新日期：2016-08-01 00:00:00

abstract：:Hybrid Shiga toxin-producing Escherichia coli (STEC) and uropathogenic E. coli (UPEC) strains of multilocus sequence type 141 (ST141) cause both urinary tract infections and diarrhea in humans and are phylogenetically positioned between STEC and UPEC strains. We used comparative genomic analysis of 85 temporally and s...

journal_title：Journal of clinical microbiology

authors： Gati NS,Middendorf-Bauchart B,Bletz S,Dobrindt U,Mellmann A

更新日期：2019-12-23 00:00:00

abstract：:A total of 233 isolates of Pasteurella multocida were obtained from 2,912 cases of clinical respiratory disease in pigs in China, giving an isolation rate of 8.0%. Serogroup A P. multocida isolates were isolated from 92 cases (39.5%), and serogroup D isolates were isolated from 128 cases (54.9%); 12 isolates (5.2%) we...

journal_title：Journal of clinical microbiology

authors： Tang X,Zhao Z,Hu J,Wu B,Cai X,He Q,Chen H

更新日期：2009-04-01 00:00:00

abstract：:The National Committee for Clinical Laboratory Standards has recently changed the oxacillin breakpoint from >/=4 mg/liter to >/=0. 5 mg/liter to detect methicillin-resistant coagulase-negative staphylococci (CoNS) because the previous breakpoint lacked sensitivity. To determine the correlation between the new oxacilli...

journal_title：Journal of clinical microbiology

authors： Hussain Z,Stoakes L,Massey V,Diagre D,Fitzgerald V,El Sayed S,Lannigan R

更新日期：2000-02-01 00:00:00

abstract：:From November 2006 to April 2007, nine nonrepetitive isolates of Klebsiella pneumoniae with reduced susceptibility or resistance to carbapenems were recovered from clinical specimens from separate patients hospitalized in a tertiary care hospital. The imipenem-EDTA synergy test was positive for all isolates. PCR, sequ...

journal_title：Journal of clinical microbiology

authors： Tokatlidou D,Tsivitanidou M,Pournaras S,Ikonomidis A,Tsakris A,Sofianou D

更新日期：2008-03-01 00:00:00

abstract：:Microsporidia are protozoa parasites responsible for significant gastrointestinal disease in patients infected with human immunodeficiency virus. We evaluated a PCR assay of stool samples, duodenal aspirates, and biopsy specimens from patients with Enterocytozoon bieneusi infection. A 210-bp DNA fragment of the unique...

journal_title：Journal of clinical microbiology

authors： Velásquez JN,Carnevale S,Guarnera EA,Labbé JH,Chertcoff A,Cabrera MG,Rodríguez MI

更新日期：1996-12-01 00:00:00

abstract：:Nineteen isolates of leptospires recovered from patients during three epidemics that occurred at different places and different times in the Andaman Islands and eight isolates from sporadic cases were characterized using serological and molecular genetic techniques. Group sera and monoclonal antibodies were used for a...

journal_title：Journal of clinical microbiology

authors： Vijayachari P,Ahmed N,Sugunan AP,Ghousunnissa S,Rao KR,Hasnain SE,Sehgal SC

更新日期：2004-08-01 00:00:00

abstract：:We sequenced homologous chromosomal loci from several North American and European isolates of the Lyme disease spirochete Borrelia burgdorferi, as well as from the relapsing fever spirochete Borrelia hermsii. Inter- and intraspecies sequence comparisons permitted the design of B. burgdorferi-specific polymerase chain ...

journal_title：Journal of clinical microbiology

authors： Rosa PA,Hogan D,Schwan TG

更新日期：1991-03-01 00:00:00

abstract：:An enzymatic profile of 20 strains of Pseudomonas maltophilia was undertaken with conventional plate tests, API ZYM, and 4-methylumbelliferyl-conjugated substrates. All strains produced DNase, RNase, arbutinase, esterases and lipases, mucinase, acid and alkaline phosphatases, alkaline pyrophosphate diesterase, phospho...

journal_title：Journal of clinical microbiology

authors： O'Brien M,Davis GH

更新日期：1982-09-01 00:00:00

abstract：:The development of a novel normalized quantitative competitive real-time PCR on the LightCycler instrument (NQC-LC-PCR) and its application to the quantification of cytomegalovirus (CMV) DNA in clinical samples are described. A heterologous competitor DNA was spiked into test samples and served as an internal amplific...

journal_title：Journal of clinical microbiology

authors： Stöcher M,Berg J

更新日期：2002-12-01 00:00:00

abstract：:Comprehensive data on the prevalence of quinolone resistance in Mycobacterium tuberculosis clinical isolates in the United States are scarce. By use of a systematic population-based approach, M. tuberculosis strains from tuberculosis (TB) cases were collected in Harris County, TX, in 2007 to 2008. The susceptibilities...

journal_title：Journal of clinical microbiology

authors： El Sahly HM,Teeter LD,Jost KC Jr,Dunbar D,Lew J,Graviss EA

更新日期：2011-08-01 00:00:00

abstract：:A biotin-avidin-amplified indirect immunofluorescence method was used to demonstrate specific serum immunoglobulin M (IgM) antibodies in nephropathia epidemica, the Scandinavian type of hemorrhagic fever with renal syndrome. The antigen in the test was the cross-reacting agent of Korean hemorrhagic fever, Hantaan viru...

journal_title：Journal of clinical microbiology

authors： Settergren B,Juto P,Wadell G

更新日期：1987-06-01 00:00:00

abstract：:Zika virus (ZIKV) is a mosquito-borne flavivirus that is responsible for recent explosive epidemics in the Americas. Notably, ZIKV infection during pregnancy has been found to cause congenital birth defects, including microcephaly, and ZIKV has been associated with Guillain-Barré syndrome in adults. Diagnosis and surv...

journal_title：Journal of clinical microbiology

authors： Balmaseda A,Zambrana JV,Collado D,García N,Saborío S,Elizondo D,Mercado JC,Gonzalez K,Cerpas C,Nuñez A,Corti D,Waggoner JJ,Kuan G,Burger-Calderon R,Harris E

更新日期：2018-02-22 00:00:00

abstract：:Conserved primers were used to PCR amplify 95% of the Helicobacter hepaticus 16S rRNA gene. Its sequence was determined and aligned to those of related bacteria, enabling the selection of primers to highly diverged regions of the 16S rRNA gene and an oligonucleotide probe for the development of a PCR-liquid hybridizat...

journal_title：Journal of clinical microbiology

authors： Battles JK,Williamson JC,Pike KM,Gorelick PL,Ward JM,Gonda MA

更新日期：1995-05-01 00:00:00

abstract：:Aspergillus terreus isolates collected from patients at The M. D. Anderson Cancer Center in Houston, TX, and at The University Hospital of Innsbruck, Austria, were analyzed using random amplification of polymorphic DNA-PCR with three different primers. No strain similarity in either institution was detected, indicatin...

journal_title：Journal of clinical microbiology

authors： Lass-Flörl C,Grif K,Kontoyiannis DP

更新日期：2007-08-01 00:00:00

abstract：:The accuracy of the new API YeastIdent system and the Flow Laboratories Uni-Yeast-Tek identification kit with an expanded data base was evaluated in comparison to the API 20C yeast identification system by three laboratories. A total of 489 test isolates were used, biased toward yeasts commonly encountered in clinical...

journal_title：Journal of clinical microbiology

authors： Salkin IF,Land GA,Hurd NJ,Goldson PR,McGinnis MR

更新日期：1987-04-01 00:00:00

abstract：:We report the isolation of Pseudomonas putrefaciens from an intra-abdominal abscess in a patient with colonic carcinoma and from bile in two patients with biliary tract disease. In all three cases, P. putrefaciens was isolated in mixed culture with enteric bacteria. ...

journal_title：Journal of clinical microbiology

authors： Marne C,Pallarés R,Sitges-Serra A

更新日期：1983-06-01 00:00:00

abstract：:Group G Streptococcus has been implicated as a causative agent of pharyngitis in outbreak situations, but its role in endemic disease remains elusive. We found an unexpected inverse association of Streptococcus dysgalactiae subsp. equisimilis colonization and sore throat in a study of 2,194 children of 3 to 15 years o...

journal_title：Journal of clinical microbiology

authors： Tartof SY,Farrimond F,de Matos JA,Reis JN,Ramos RT,Andrade AN,dos Reis MG,Riley LW

更新日期：2011-01-01 00:00:00