Abstract:
:We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suitable for routine clinical diagnostics.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Hrncirova K,Lengerova M,Kocmanova I,Racil Z,Volfova P,Palousova D,Moulis M,Weinbergerova B,Winterova J,Toskova M,Pospisilova S,Mayer Jdoi
10.1128/JCM.01109-10subject
Has Abstractpub_date
2010-09-01 00:00:00pages
3392-4issue
9eissn
0095-1137issn
1098-660Xpii
JCM.01109-10journal_volume
48pub_type
杂志文章abstract::The AutoMicrobic system (AMS) is capable of identifying most Enterobacteriaceae within 8 h and many glucose-nonfermenting, gram-negative bacilli after 13 h of incubation. Early preliminary results can be readily obtained from the computer as the tests incubate. Data with 1,023 bacterial isolates were reviewed to deter...
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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