Transmission of an azole-resistant isogenic strain of Candida albicans among human immunodeficiency virus-infected family members with oropharyngeal candidiasis.

abstract：:Standardization of sequence chromatogram analysis is required for consistent genotypic tropism determination across laboratories. A freely available, fast, and automated chromatogram analysis tool (RECall) provided tropism interpretations equivalent to those of manual sequence editing of 521 V3 loop HIV-1 population s...

journal_title：Journal of clinical microbiology

authors： Pou C,Bellido R,Casadellà M,Puig T,Clotet B,Harrigan R,Paredes R

更新日期：2013-08-01 00:00:00

abstract：:Assessment of cure of Trypanosoma cruzi infection by antibody seroconversion usually involves several years of follow-up. Parasitological negativity is useless for cure assessment, since even untreated patients mostly show negative results; conversely, positive tests are of great value because they indicate treatment ...

journal_title：Journal of clinical microbiology

authors： Galvão LM,Chiari E,Macedo AM,Luquetti AO,Silva SA,Andrade AL

更新日期：2003-11-01 00:00:00

abstract：:We compared the BD Phoenix automated microbiology system to the Bruker Biotyper (version 2.0) matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) system for identification of gram-negative bacilli, using biochemical testing and/or genetic sequencing to resolve discordant resul...

journal_title：Journal of clinical microbiology

authors： Saffert RT,Cunningham SA,Ihde SM,Jobe KE,Mandrekar J,Patel R

更新日期：2011-03-01 00:00:00

abstract：:Well-defined Legionella pneumophila strains were analyzed by amplification of variable genomic regions with arbitrary and repeat sequence primers. Clinical and environmental outbreak-related isolates showed closely related amplicon patterns. Eleven strains of unrelated origins displayed 10 distinct patterns. Fingerpri...

journal_title：Journal of clinical microbiology

authors： van Belkum A,Struelens M,Quint W

更新日期：1993-08-01 00:00:00

abstract：:In order to better understand the role of Mycobacterium leprae nasal carriage in the maintenance of infection reservoirs and transmission of leprosy, we applied a polymerase chain reaction (PCR) that detected a 531-bp fragment of the pra gene of M. leprae on nasal swab specimens collected through a total population su...

journal_title：Journal of clinical microbiology

authors： Klatser PR,van Beers S,Madjid B,Day R,de Wit MY

更新日期：1993-11-01 00:00:00

abstract：:Group A streptococci and enterococci were identified by two L-pyrrolidonyl-beta-naphthylamide (PYR) hydrolysis tests. One uses Minitek PYR disks (BBL Microbiology Systems, Cockeysville, Md.); the other uses a swab impregnated with PYR reagent. Both tests and Strep-A-Chek (EY Laboratories, San Mateo, Calif.), a commerc...

journal_title：Journal of clinical microbiology

authors： Wellstood SA

更新日期：1987-09-01 00:00:00

abstract：:A cross-reaction between Acinetobacter calcoaceticus and chlamydiae is described. A water-soluble, heat stable, non-dialyzable antigen was extracted from Acinetobacter species by boiling. This antigen fixed complement in the presence of homologous hyperimmune sera from rabbits or guinea pigs and in the presence of het...

journal_title：Journal of clinical microbiology

authors： Brade H,Brunner H

更新日期：1979-12-01 00:00:00

abstract：:Lipoglycans from mycoplasmas possess the ability to induce the elaboration of procoagulant activity. This represents a third endotoxic property for these polymers, in addition to the pyrogenicity and clotting of Limulus lysate reported previously. Lipoglycans from three species of Acholeplasma (A. axanthum, A. granula...

journal_title：Journal of clinical microbiology

authors： Miragliotta G,Barone G,Monno RA,Fumarola D,Smith PF

更新日期：1987-07-01 00:00:00

abstract：:The development of a rapid test to identify Mycobacterium tuberculosis Beijing isolates and specifically strain GC1237, coming from a sub-Saharan country, is needed due to its alarming wide spread on Gran Canaria Island (Spain). A rapid test that detects IS6110 present between dnaA and dnaN in the Beijing strains and ...

journal_title：Journal of clinical microbiology

authors： Millán-Lou MI,Alonso H,Gavín P,Hernández-Febles M,Campos-Herrero MI,Copado R,Cañas F,Kremer K,Caminero JA,Martín C,Samper S

更新日期：2012-02-01 00:00:00

abstract：:Johne's disease (JD) is an economically important infectious disease in livestock farming caused by Mycobacterium avium subsp. paratuberculosis As an alternative to serological tests, which are used mainly for the screening of whole herds, we developed a novel ResoLight-based real-time PCR (RL-PCR) assay with pooled f...

journal_title：Journal of clinical microbiology

authors： Kawaji S,Nagata R,Minegishi Y,Saruyama Y,Mita A,Kishizuka S,Saito M,Mori Y

更新日期：2020-11-18 00:00:00

abstract：:Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease with a high case fatality risk and is caused by the SFTS virus (SFTSV). A retrospective study conducted after the first identification of an SFTS patient in Japan revealed that SFTS is endemic to the region, and the virus exists indig...

journal_title：Journal of clinical microbiology

authors： Yoshikawa T,Fukushi S,Tani H,Fukuma A,Taniguchi S,Toda S,Shimazu Y,Yano K,Morimitsu T,Ando K,Yoshikawa A,Kan M,Kato N,Motoya T,Kuzuguchi T,Nishino Y,Osako H,Yumisashi T,Kida K,Suzuki F,Takimoto H,Kitamoto H,Ma

更新日期：2014-09-01 00:00:00

abstract：:A production model of the semi-automated PASCO MIC-ID system (PASCO Laboratories, Wheat Ridge, Colo.) was evaluated with 122 groups or species of gram-negative bacilli, which included typical (499 cultures) and atypical (37 cultures) strains of fermenters and nonfermenters. The PASCO system identified 90.9% of 536 cul...

journal_title：Journal of clinical microbiology

authors： Rhoden DL,Schable B,Smith PB

更新日期：1987-12-01 00:00:00

abstract：:Group B Streptococcus detection directly from Copan ESwab collected samples, using the BD Max GBS assay, was evaluated on receipt in the laboratory and after 24 h at room temperature. Results were compared to those using Lim broth enrichment PCR and culture. No significant difference was observed between 24 h ESwab an...

journal_title：Journal of clinical microbiology

authors： Silbert S,Rocchetti TT,Gostnell A,Kubasek C,Widen R

更新日期：2016-06-01 00:00:00

abstract：:We serotyped 222 Pasteurella multocida strains isolated from swine lungs at slaughter. Capsular serotypes A and D were determined by the hyaluronidase sensitivity and acriflavin agglutination tests, respectively. Somatic antigens were determined by gel diffusion against standard antisera. Capsular serotype A was found...

journal_title：Journal of clinical microbiology

authors： Pijoan C,Morrison RB,Hilley HD

更新日期：1983-06-01 00:00:00

abstract：:We compared putative molecular markers of virulence (vacA, cagA, and iceA) of Helicobacter pylori strains isolated from 52 adult duodenal ulcer patients from West Bengal, India, with those of H. pylori strains isolated from 48 adult healthy volunteers from the same region. On the basis of genotyping by PCR, we conclud...

journal_title：Journal of clinical microbiology

authors： Chattopadhyay S,Datta S,Chowdhury A,Chowdhury S,Mukhopadhyay AK,Rajendran K,Bhattacharya SK,Berg DE,Nair GB

更新日期：2002-07-01 00:00:00

abstract：:Burkholderia pseudomallei is the causative agent of melioidosis. This condition most often presents as pneumonia and bacteremia, with mortality rates of 9% to 70%. Therefore, early identification of this organism may aid in directing appropriate management. This study aimed to use the Vitek matrix-assisted laser desor...

journal_title：Journal of clinical microbiology

authors： Gassiep I,Armstrong M,Norton RE

更新日期：2019-04-26 00:00:00

abstract：:Lymphadenopathy (LN) is the most common clinical manifestation of acute acquired toxoplasma infection in humans. The diagnosis of toxoplasmic lymphadenitis (TL) is established by serological methods and/or lymph node biopsy. In the United States, the differential agglutination (of acetone [AC]-fixed versus that of for...

journal_title：Journal of clinical microbiology

authors： Montoya JG,Berry A,Rosso F,Remington JS

更新日期：2007-05-01 00:00:00

abstract：:To simplify the serotyping of Streptococcus pneumoniae, a dot bot assay has been developed and compared with the standard quellung reaction in 1,082 isolates. The technique has been demonstrated to be sensitive, specific, easy to perform, and inexpensive. The dot blot assay could be useful when large numbers of pneumo...

journal_title：Journal of clinical microbiology

authors： Fenoll A,Jado I,Vicioso D,Casal J

更新日期：1997-03-01 00:00:00

abstract：:Sera from patients with paracoccidioidomycosis (PCM), histoplasmosis (HP), or Jorge Lobo's disease (JL) were titrated against purified gp43 from Paracoccidioides brasiliensis by using both enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation (IPP) reactions with 125I-labeled antigens. In IPP, PCM sera and...

journal_title：Journal of clinical microbiology

authors： Puccia R,Travassos LR

更新日期：1991-08-01 00:00:00

abstract：:The actinomycete Rhodococcus equi is an important pathogen of horses and an emerging opportunistic pathogen of humans. Identification of R. equi by classical bacteriological techniques is sometimes difficult, and misclassification of an isolate is not uncommon. We report here on a specific PCR assay for the rapid and ...

journal_title：Journal of clinical microbiology

authors： Ladrón N,Fernández M,Agüero J,González Zörn B,Vázquez-Boland JA,Navas J

更新日期：2003-07-01 00:00:00

abstract：:A simple, relatively rapid silver impregnation stain has been found to stain Legionella pneumophila effectively in paraffin-embedded tissue sections while permitting visualization of histological detail. It may also be used to stain the organism in body fluids. The stain is not specific and thus must be confirmed by d...

journal_title：Journal of clinical microbiology

authors： Faine S,Edelstein P,Kirby BD,Finegold SM

更新日期：1979-07-01 00:00:00

abstract：:Early detection of Mycobacterium tuberculosis complex (MTBC) and markers conveying drug resistance can have a beneficial impact on preventive public health actions. We describe here a new molecular point-of-care (POC) system, the Genedrive, which is based on simple sample preparation combined with PCR to detect MTBC a...

journal_title：Journal of clinical microbiology

authors： Castan P,de Pablo A,Fernández-Romero N,Rubio JM,Cobb BD,Mingorance J,Toro C

更新日期：2014-02-01 00:00:00

abstract：:Immune pressure on lentiviruses exerted by cytotoxic T lymphocytes (CTL) selects for virus CTL epitope mutations. Currently employed methods for monitoring emerging CTL epitope mutations rely on the labor-intensive and time-consuming techniques of virus population or clonal sequencing. Here we describe the development...

journal_title：Journal of clinical microbiology

authors： Peyerl FW,Barouch DH,Bazick HS,Manuel E,Letvin NL

更新日期：2005-09-01 00:00:00

abstract：:The objective of this study was to measure the performance of the Affirm Ambient Temperature Transport System (ATTS) over time and to estimate the length of time the system can preserve a vaginal specimen containing the three common organisms causing vaginitis: Trichomonas vaginalis, Candida species, and Gardnerella v...

journal_title：Journal of clinical microbiology

authors： Brown HL,Fuller DA,Davis TE,Schwebke JR,Hillier SL

更新日期：2001-09-01 00:00:00

abstract：:Mitochondrial cytochrome b (cyt b) genes of 42 strains representing 23 species of the genus Trichosporon were partially sequenced to determine their molecular phylogenetic relationships. Almost half of the 22 strains investigated (from 11 different species) contained introns in their sequences. Analysis of a 396-bp co...

journal_title：Journal of clinical microbiology

authors： Biswas SK,Wang L,Yokoyama K,Nishimura K

更新日期：2005-10-01 00:00:00

abstract：:Cell line 293, a continuous line of transformed human embryonic kidney cells, has been recognized for its sensitivity in the isolation of adenoviruses, particularly the fastidious species 40 and 41, from stool specimens. To explore the possibility of using this cell line for the isolation of other viruses from clinica...

journal_title：Journal of clinical microbiology

authors： Brown M,Petric M

更新日期：1986-04-01 00:00:00

abstract：:Clinical and experimental observations suggest that immune globulin may prevent otitis media (OM) in children. We performed experiments in chinchillas to test the hypothesis that human bacterial polysaccharide immune globulin (BPIG) might prevent OM caused by Streptococcus pneumoniae. Animals were given BPIG or saline...

journal_title：Journal of clinical microbiology

authors： Shurin PA,Giebink GS,Wegman DL,Ambrosino D,Rholl J,Overman M,Bauer T,Siber GR

更新日期：1988-04-01 00:00:00

abstract：:The Porphyromonas gingivalis collagenase-specific serum immunoglobulin A (IgA), IgM, and IgG responses from 20 patients with early-onset periodontitis (EOP), 20 patients with adult periodontitis, (AP), and 20 age- and sex-matched healthy controls were examined by immunoblot analysis. A recombinant collagenase antigen ...

journal_title：Journal of clinical microbiology

authors： Wittstock M,Flemmig TF,Schmidt H,Mutters R,Karch H

更新日期：1996-10-01 00:00:00

abstract：:Pathozyme-Myco G (Myco G), M, A, and TB complex plus (Omega Diagnostics Ltd., Alloa, Scotland) were evaluated for the serological diagnosis of pulmonary tuberculosis (TB) in an Argentinean population. Sera from 58 patients with pulmonary TB, 24 subjects with pulmonary mycobacteriosis or mycoses (pulmonary MM group), a...

journal_title：Journal of clinical microbiology

authors： Imaz MS,Comini MA,Zerbini E,Sequeira MD,Latini O,Claus JD,Singh M

更新日期：2004-02-01 00:00:00

abstract：:Direct heteroduplex analysis and a universal heteroduplex generator assay were performed to detect rifampin resistance rapidly in Turkish Mycobacterium tuberculosis isolates. Cross-resistance to rifapentine, rifabutin, and rifalazil was investigated. A relationship between specific mutations and resistance patterns, w...

journal_title：Journal of clinical microbiology

authors： Saribaş Z,Kocagöz T,Alp A,Günalp A

更新日期：2003-02-01 00:00:00