Abstract:
:Thirteen different serogroup strains of Yersinia pseudotuberculosis and two strains of Yersinia enterocolitica O:3 were examined for the presence of plasmids and plasmid-mediated properties, calcium growth dependency, and autoagglutination. Two Y. enterocolitica strains and eight serogroup (IA, IIA, IIC, III, IVA, VB, VI, and VIII) strains, except for five serogroups (IB, IIB, IVB, VA, and VII), of Y. pseudotuberculosis harbored plasmids ranging in molecular size from 27 to 115 kilobases. Filter hybridization of restriction endonuclease-digested plasmid DNA from different serogroup strains indicated that all plasmid DNAs conferring calcium growth dependency and autoagglutination shared a high degree of DNA sequence homology, regardless of the different serogroups of Y. pseudotuberculosis and Y. enterocolitica.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Ishiguro N,Nakaoka Y,Sato G,Tsubokura Mdoi
10.1128/JCM.21.4.662-665.1985subject
Has Abstractpub_date
1985-04-01 00:00:00pages
662-5issue
4eissn
0095-1137issn
1098-660Xjournal_volume
21pub_type
杂志文章abstract::Nocardia infection following anterior cruciate ligament (ACL) allograft reconstruction is a rare occurrence. We report a case of Nocardia infection of an allograft ACL reconstruction and septic arthritis of the knee joint due to an organism most similar to the novel Nocardia species Nocardia aobensis. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00754-15
更新日期:2015-08-01 00:00:00
abstract::A total of 50 strains of Staphylococcus aureus, including 41 methicillin-resistant S. aureus (MRSA) strains, were characterized by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins. The protein patterns contained 40-50 discrete bands and were highly reproducible. Partial ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.27.11.2574-2581.1989
更新日期:1989-11-01 00:00:00
abstract::Etest is a new concept for MIC determinations for antimicrobial agents that is based on a predefined antibiotic gradient on a plastic strip calibrated with a continuous logarithmic MIC scale covering 15 twofold dilutions. Etest was compared with a reference agar dilution method for susceptibility testing of clinical i...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.8.1846-1849.1994
更新日期:1994-08-01 00:00:00
abstract::Detection of herpes simplex virus in clinical specimens by the suspension-infection technique was compared with detection by conventional cell culture. The sensitivity and specificity of the suspension-infection technique compared with those of conventional culture were 95.9 to 98.2% and 97.5 to 100%, respectively, de...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.29.7.1554-1557.1991
更新日期:1991-07-01 00:00:00
abstract::"Campylobacter pyloridis" has been recently described as a gastritis-associated bacterium. We studied 20 strains. The bacteria had most of the characteristics of Campylobacter spp. strains. They were hippurate negative and tolerant to triphenyl tetrazolium chloride (at 0.4 and 1 mg/ml). They grew on all the media comm...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.22.6.1007-1010.1985
更新日期:1985-12-01 00:00:00
abstract::The microbiological and molecular characteristics of the rickettsiae isolated from humans with Queensland tick typhus (QTT) caused by Rickettsia australis and the recently described Flinders Island spotted fever (FISF) were compared. Clinically and serologically, the diseases are similar. Cell culture reveals differen...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.30.11.2896-2902.1992
更新日期:1992-11-01 00:00:00
abstract::Marburg virus (MARV) causes a severe hemorrhagic fever in humans with a high mortality rate. The rapid and accurate identification of the virus is required to appropriately provide infection control and outbreak management. Here, we developed and evaluated a one-step reverse transcription-loop-mediated isothermal ampl...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01224-09
更新日期:2010-07-01 00:00:00
abstract::Antiserum to a purified type R lipopolysaccharide antigen isolated from Neisseria gonorrhoeae was used in a slide agglutination test and compared with conventional carbohydrate utilization and fluorescent antibody tests to confirm the identity of laboratory cultures classified as typical or "atypical" N. gonorrhoeae. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1979-04-01 00:00:00
abstract::The Porphyromonas gingivalis collagenase-specific serum immunoglobulin A (IgA), IgM, and IgG responses from 20 patients with early-onset periodontitis (EOP), 20 patients with adult periodontitis, (AP), and 20 age- and sex-matched healthy controls were examined by immunoblot analysis. A recombinant collagenase antigen ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.34.10.2411-2413.1996
更新日期:1996-10-01 00:00:00
abstract::The diagnostic value of RT-PCR of amniotic fluid (AF) for prenatal diagnosis of congenital rubella virus infection in 45 pregnant women with confirmed primary infection was assessed. Specificity of RT-PCR was 100% and sensitivity ranged between 83 and 95%. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.10.4818-4820.2004
更新日期:2004-10-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.34.7.1819-1820.1996
更新日期:1996-07-01 00:00:00
abstract::Despite the high virological response rates achieved with current directly acting antiviral agents (DAAs) against hepatitis C virus (HCV), around 2% to 5% of treated patients do not achieve a sustained viral response. The identification of amino acid substitutions associated with treatment failure requires analytical ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01985-20
更新日期:2020-11-18 00:00:00
abstract::Species of dermatophytes are classified into three anamorphic (asexual) genera, Epidermophyton, Microsporum, and Trichophyton. Conventional methods used to identify dermatophytes are often lengthy and may be inconclusive because of atypical microscopic or colony morphology. Based on the internal transcribed spacer 1 (...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00829-07
更新日期:2007-10-01 00:00:00
abstract::The Gen-Probe Amplified Mycobacterium tuberculosis direct test is widely used in the diagnosis of tuberculosis. Specimens may contain amplification inhibitors, potentially leading to false-negative results if unrecognized. We report a failure to detect inhibition, despite adherence to the inhibition testing guidelines...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00966-09
更新日期:2009-09-01 00:00:00
abstract::A TaqMan PCR was established for identification and quantitation of members of the Ehrlichia phagocytophila group in experimentally infected cows and in Ixodes ricinus ticks. The TaqMan PCR identified a 106-bp section of the 16S rRNA gene by use of a specific fluorogenic probe and two primers. This technique was speci...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.5.1329-1331.1999
更新日期:1999-05-01 00:00:00
abstract::Detection of group B streptococcus (GBS) antigen in urine by latex particle agglutination (LPA) may facilitate the rapid diagnosis of GBS sepsis. We sought to compare three commercial LPA assays with specimens that were spiked with type-specific antigen, group-specific antigen, or type III organisms. There were sensit...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.29.12.2895-2896.1991
更新日期:1991-12-01 00:00:00
abstract::Tracking the spread of noroviruses during outbreaks of gastroenteritis is hampered by the lack of sequence diversity in those regions of the genome chosen for virus detection and characterization. Sequence analysis of regions of the genes encoding the RNA-dependent RNA polymerase and the S domain of the capsid does no...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02240-07
更新日期:2008-03-01 00:00:00
abstract::To diagnose catheter-related sepsis without removing the catheter, we evaluated the differential positivity times of cultures of blood drawn simultaneously from central venous catheter and peripheral sites. In a 450-bed cancer reference center, simultaneous central- and peripheral-blood cultures were prospectively per...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.36.1.105-109.1998
更新日期:1998-01-01 00:00:00
abstract::Three molecular typing methods were used to study the relationships among 184 Campylobacter strains isolated from humans, cattle, and chickens. All strains were genotyped by amplified fragment length polymorphism (AFLP) analysis, multilocus sequence typing (MLST), and sequence analysis of a genomic region with short t...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.1.15-26.2003
更新日期:2003-01-01 00:00:00
abstract::Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae generally cannot be treated with penicillins and cephalosporins. However, some later-generation cephalosporins, including cefepime, are poorly hydrolyzed by specific ESBL enzymes, and certain strains demonstrate in vitro susceptibility to these agent...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2017-02-01 00:00:00
abstract::For prevention and control of gonorrhea, an objective, highly discriminating, and reproducible molecular epidemiological characterization of Neisseria gonorrhoeae is essential. In the present study, in pursuance of providing such qualities, pyrosequencing technology, a fast real-time DNA sequence analysis, was applied...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.7.2926-2934.2004
更新日期:2004-07-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.38.3.1191-1195.2000
更新日期:2000-03-01 00:00:00
abstract::One hundred and twenty-seven apyocyanogenic fluorescent Pseudomonas strains capable of growth at 41 degrees C, but differing from Pseudomonas aeruginosa, were typed serologically and tested for pyocin production, antibiotic susceptibility, selected biochemical reactions, and utilization of selected substrates. Results...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
abstract::Matched vaginal and cervical specimens from 96 subjects were analyzed by quantitative PCR for the presence and concentration of bacterial vaginosis-associated microbes and commensal Lactobacillus spp. Detection of these microbes was 92% concordant, indicating that microbial floras at these body sites are generally sim...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00795-14
更新日期:2014-08-01 00:00:00
abstract::The aims of this study were to evaluate the use of molecular fingerprinting for assessment of bacterial diversity in canine duodenal juice and to evaluate the variation in the small intestinal microflora at repeated sampling. Two groups of dogs were used. Duodenal juice was collected from eight dogs euthanized for an ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.10.4702-4708.2004
更新日期:2004-10-01 00:00:00
abstract::We describe a simple method for the rapid detection of bovine viral diarrhea virus (BVDV) that uses a one-tube reverse transcription PCR (RT-PCR) and total RNA extracted directly from a variety of bovine specimens, including whole blood and tissues. Reagents for both RT and PCR were combined in a one-tube, single-buff...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.33.2.287-291.1995
更新日期:1995-02-01 00:00:00
abstract::Antibiotic neutralization in blood culture media from two automated systems was evaluated by measuring the recovery of organisms and times to detection in simulated cultures. Overall, BD Bactec Plus media (Bactec FX system) outperformed TREK 80 ml Redox media (VersaTREK system), although results suggest a relative rat...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01958-10
更新日期:2011-04-01 00:00:00
abstract::Malaria-specific rapid diagnostic tests (RDTs) targeting aldolase show highly variable sensitivities. We assessed diversity in Plasmodium falciparum and P. vivax aldolases by sequencing the coding genes from parasites of various origins. The results show that aldolases are highly conserved, indicating that antigenic d...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01611-06
更新日期:2006-12-01 00:00:00
abstract::We report a case in which fecal microbiota transplantation (FMT) utilized for relapsing Clostridium difficile colitis successfully eradicated colonization with several multidrug-resistant organisms (MDROs). FMT may have an additive benefit of reducing MDRO carriage and should be further investigated as a potential mea...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00820-15
更新日期:2015-06-01 00:00:00
abstract::The ability of MicroScan rapid panels (Baxter MicroScan, West Sacramento, Calif.) to detect oxacillin resistance in 92 clinical isolates of Staphylococcus aureus and 103 coagulase-negative staphylococci was evaluated by comparing results with those of MicroScan 24-h MIC panels and, to resolve discrepancies, oxacillin ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.4.1058-1059.1994
更新日期:1994-04-01 00:00:00