Identification of dermatophytes by an oligonucleotide array.

abstract：:Global HIV treatment programs need sensitive and affordable tests to monitor HIV drug resistance. We compared mutant detection by the oligonucleotide ligation assay (OLA), an economical and simple test, to massively parallel sequencing. Nonnucleoside reverse transcriptase inhibitor (K103N, V106M, Y181C, and G190A) and...

journal_title：Journal of clinical microbiology

authors： Beck IA,Deng W,Payant R,Hall R,Bumgarner RE,Mullins JI,Frenkel LM

更新日期：2014-07-01 00:00:00

abstract：:A 44-year-old diabetic female presented to a hospital in Jamaica with thermal burns. Trichosporon asahii was isolated from facial wounds, sputum, and a meningeal swab. Dissemination of the fungus was demonstrated in stained histological sections of the meninges and a brain abscess at autopsy. Pure growth of the fungus...

journal_title：Journal of clinical microbiology

authors： Heslop OD,Nyi Nyi MP,Abbott SP,Rainford LE,Castle DM,Coard KC

更新日期：2011-12-01 00:00:00

abstract：:The Y448H mutation in NS5B has been selected by GS-9190 as well as several benzothiadiazine hepatitis C virus (HCV) polymerase inhibitors in vitro and in vivo. However, the level and the evolution kinetics of this resistance mutation prior to and during treatment are poorly understood. In this study, we developed an a...

journal_title：Journal of clinical microbiology

authors： Bae AS,Ku KS,Miller MD,Mo H,Svarovskaia ES

更新日期：2011-09-01 00:00:00

abstract：:We developed an accurate nonradioactive colony hybridization assay (NCHA) using a digoxigenin-labeled polynucleotide probe and an antidigoxigenin alkaline phosphatase conjugate for the identification of enterotoxigenic Escherichia coli (ETEC) harboring genes for colonization factor antigen I (CFA/I), coli surface anti...

journal_title：Journal of clinical microbiology

authors： Sommerfelt H,Grewal HM,Gaastra W,Svennerholm AM,Bhan MK

更新日期：1992-07-01 00:00:00

abstract：:A total of 4,409 stool specimens from infants less than 5 years of age seeking treatment for diarrhea in Matlab, Bangladesh, were tested for the presence of adenoviruses by using an enzyme immunoassay (EIA). EIA-positive stool samples were serotyped with monoclonal antibodies specific for adenovirus type 40 (Ad40) and...

journal_title：Journal of clinical microbiology

authors： Jarecki-Khan K,Tzipori SR,Unicomb LE

更新日期：1993-03-01 00:00:00

abstract：:A prereduced, anaerobically sterilized system of tubed media (PRAS II; Scott Laboratories, Fiskeville, R.I.) was evaluated for accuracy in the identification of anerobic microorganisms. PRAS II was found to be a rapid and accurate identification system for obligate anaerobes which does not require the use of gas cannu...

journal_title：Journal of clinical microbiology

authors： Beaucage CM,Onderdonk AB

更新日期：1982-09-01 00:00:00

abstract：:A monoclonal antibody (MAb) immunoglobulin G2a (2125) was produced against a 60-kDa Legionella heat shock protein (HSP), recognizing a unique epitope common to all species of the genus Legionella. The antibody reacted in the immunoblot with 59 Legionella species and serogroups that were tested and showed no cross-reac...

journal_title：Journal of clinical microbiology

authors： Steinmetz I,Rheinheimer C,Hübner I,Bitter-Suermann D

更新日期：1991-02-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay was developed for the detection of circulating free and complexed staphylococcal antigens in various clinical categories of staphylococcal infections. Circulating immune complexes were studied by the polyethylene glycol precipitation method. Circulating immune complexes and staphyl...

journal_title：Journal of clinical microbiology

authors： Lentino JR,Rytel MW

更新日期：1982-12-01 00:00:00

abstract：:Rapid diagnostic tests (RDTs) based on the detection of specific antibodies in serum are commonly used for the diagnosis of visceral leishmaniasis (VL). Several commercial kits are available, and some of them allow the use of whole-blood samples instead of serum. An RDT is much more user-friendly for blood samples tha...

journal_title：Journal of clinical microbiology

authors： Kumar D,Khanal B,Tiwary P,Mudavath SL,Tiwary NK,Singh R,Koirala K,Boelaert M,Rijal S,Sundar S

更新日期：2013-12-01 00:00:00

abstract：:In the treatment of serious bacterial infections, the rapid institution of appropriate antimicrobial chemotherapy may be lifesaving. Choosing the correct antibiotic or combination of antibiotics is becoming very important, as multidrug resistance is found in many pathogens. Using a collection of 75 well-characterized ...

journal_title：Journal of clinical microbiology

authors： Hujer KM,Hujer AM,Endimiani A,Thomson JM,Adams MD,Goglin K,Rather PN,Pennella TT,Massire C,Eshoo MW,Sampath R,Blyn LB,Ecker DJ,Bonomo RA

更新日期：2009-05-01 00:00:00

abstract：:In 32 patients for whom highly active antiretroviral therapy was failing, a good agreement between drug resistance-associated mutations in plasma and peripheral blood mononuclear cells (PBMCs) was found (k = 0.85). The mutations with the lowest agreement were 20R, 63P, and 84V in the protease gene and 184V in the reve...

journal_title：Journal of clinical microbiology

authors： Sarmati L,Nicastri E,Uccella I,D'Ettorre G,Parisi SG,Palmisano L,Galluzzo C,Concia E,Vullo V,Vella S,Andreoni M

更新日期：2003-04-01 00:00:00

abstract：:Standard broth microdilution (with and without bovine serum albumin [BSA] supplementation), tube dilution, and agar dilution susceptibility tests were compared for determining ramoplanin MICs. With a data base of 246 clinical isolates of gram-positive bacteria from 33 U.S. sites, it was shown that (i) agar and tube di...

journal_title：Journal of clinical microbiology

authors： Kenny MT,Brackman MA

更新日期：1994-05-01 00:00:00

abstract：:For prevention and control of gonorrhea, an objective, highly discriminating, and reproducible molecular epidemiological characterization of Neisseria gonorrhoeae is essential. In the present study, in pursuance of providing such qualities, pyrosequencing technology, a fast real-time DNA sequence analysis, was applied...

journal_title：Journal of clinical microbiology

authors： Unemo M,Olcén P,Jonasson J,Fredlund H

更新日期：2004-07-01 00:00:00

abstract：:From November 2006 to April 2007, nine nonrepetitive isolates of Klebsiella pneumoniae with reduced susceptibility or resistance to carbapenems were recovered from clinical specimens from separate patients hospitalized in a tertiary care hospital. The imipenem-EDTA synergy test was positive for all isolates. PCR, sequ...

journal_title：Journal of clinical microbiology

authors： Tokatlidou D,Tsivitanidou M,Pournaras S,Ikonomidis A,Tsakris A,Sofianou D

更新日期：2008-03-01 00:00:00

abstract：:Testing efforts for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been burdened by the scarcity of testing materials and personal protective equipment for health care workers. The simple and painless process of saliva collection allows for widespread testing, but enthusiasm is hampered by variable ...

journal_title：Journal of clinical microbiology

authors： Yee R,Truong TT,Pannaraj PS,Eubanks N,Gai E,Jumarang J,Turner L,Peralta A,Lee Y,Dien Bard J

更新日期：2021-01-21 00:00:00

abstract：:Antibiogram typing of methicillin-resistant Staphylococcus aureus with selected antibiotics was evaluated as a primary epidemiological typing tool and compared with ribotyping. Antibiograms were derived with the Kirby-Bauer disk diffusion method by using erythromycin, clindamycin, cotrimoxazole, gentamicin, and ciprof...

journal_title：Journal of clinical microbiology

authors： Blanc DS,Lugeon C,Wenger A,Siegrist HH,Francioli P

更新日期：1994-10-01 00:00:00

abstract：:We describe a central venous catheter-related (Port-A-Cath; Smiths Industries Medical Systems [SIMS] Deltec, Inc., St. Paul, Minn.) infection caused by Rhodotorula glutinis in a 51-year-old man with nasopharyngeal carcinoma. He was treated with fluconazole for 8 weeks and had the catheter removed. Two isolates of R. g...

journal_title：Journal of clinical microbiology

authors： Hsueh PR,Teng LJ,Ho SW,Luh KT

更新日期：2003-02-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been suggested as a reliable method for bacterial identification from cultures. Direct analysis of clinical samples might increase the usefulness of this method, shortening the time for microorganism identification. We comp...

journal_title：Journal of clinical microbiology

authors： Ferreira L,Sánchez-Juanes F,González-Avila M,Cembrero-Fuciños D,Herrero-Hernández A,González-Buitrago JM,Muñoz-Bellido JL

更新日期：2010-06-01 00:00:00

abstract：:The first World Health Organization (WHO) international standards (ISs) for nucleic acid amplification techniques were established two decades ago, with the initial focus on blood screening for three major viral targets, i.e., hepatitis C virus, hepatitis B virus, and human immunodeficiency virus 1. These reference ma...

journal_title：Journal of clinical microbiology

authors： Baylis SA,Wallace P,McCulloch E,Niesters HGM,Nübling CM

更新日期：2019-01-02 00:00:00

abstract：:Over a 12-month period, 43 children in eight different wards of our hospital (Hôpital Robert Debré) were infected or colonized with Klebsiella pneumoniae strains producing extended broad-spectrum beta-lactamases. The epidemiology of the outbreak was studied by a molecular approach including the determination of the be...

journal_title：Journal of clinical microbiology

authors： Bingen EH,Desjardins P,Arlet G,Bourgeois F,Mariani-Kurkdjian P,Lambert-Zechovsky NY,Denamur E,Philippon A,Elion J

更新日期：1993-02-01 00:00:00

abstract：:In a multi-health center study, a new rapid optical immunoassay (OIA) for the detection of Shiga toxin types 1 and 2, the BioStar OIA SHIGATOX kit (Inverness Medical Professional Diagnostics, Inc.), was used to prospectively screen 742 fresh fecal samples for Shiga toxins in parallel with the Premier enterohemorrhagic...

journal_title：Journal of clinical microbiology

authors： Teel LD,Daly JA,Jerris RC,Maul D,Svanas G,O'Brien AD,Park CH

更新日期：2007-10-01 00:00:00

abstract：:Infective endocarditis (IE) is a life-threatening infection of the heart endothelium and valves. Staphylococcus aureus is a predominant cause of severe IE and is frequently associated with infections in health care settings and device-related infections. Multilocus sequence typing (MLST), spa typing, and virulence gen...

journal_title：Journal of clinical microbiology

authors： Nethercott C,Mabbett AN,Totsika M,Peters P,Ortiz JC,Nimmo GR,Coombs GW,Walker MJ,Schembri MA

更新日期：2013-07-01 00:00:00

abstract：:Typing and in vitro cultivation of rotavirus-positive human stool samples stored unintentionally at ambient tropical temperatures for 2 1/2 months showed that rotavirus is stable and may still be infectious in vitro. This indicates that stool specimen collection for rotavirus studies can be performed in areas and sett...

journal_title：Journal of clinical microbiology

authors： Fischer TK,Steinsland H,Valentiner-Branth P

更新日期：2002-12-01 00:00:00

abstract：:To compare Simplexa Flu A/B & RSV PCR with cytospin-immunofluorescence and laboratory-developed TaqMan PCR methods, 445 nasopharyngeal samples were tested. Of these, 199 were positive (46 for respiratory syncytial virus [RSV], 120 for influenza A, and 33 for influenza B) and 246 were negative. The direct fluorescent-a...

journal_title：Journal of clinical microbiology

authors： Landry ML,Ferguson D

更新日期：2014-08-01 00:00:00

abstract：:We have compared the sensitivity and specificity of quantitative mycobacterial culture against results obtained by using the polymerase chain reaction for the detection of DNA from organisms of the Mycobacterium tuberculosis complex in 82 clinical specimens from patients suspected of having tuberculosis. Two amplifica...

journal_title：Journal of clinical microbiology

authors： Pierre C,Lecossier D,Boussougant Y,Bocart D,Joly V,Yeni P,Hance AJ

更新日期：1991-04-01 00:00:00

abstract：:A total of 50 strains of Staphylococcus aureus, including 41 methicillin-resistant S. aureus (MRSA) strains, were characterized by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins. The protein patterns contained 40-50 discrete bands and were highly reproducible. Partial ...

journal_title：Journal of clinical microbiology

authors： Costas M,Cookson BD,Talsania HG,Owen RJ

更新日期：1989-11-01 00:00:00

abstract：:A total of 53 strains of enterococci, including recently isolated strains with high-level resistance to vancomycin, were tested for vancomycin susceptibility by broth microdilution and disk diffusion using Mueller-Hinton media with and without supplementation with 5% blood. By using currently published parameters of t...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Hill BC,Thornsberry C

更新日期：1989-09-01 00:00:00

abstract：:Screening for HIV infection has improved since the first immunoassays. Today, diagnosis of HIV infection can be performed with fourth-generation tests that track both the patient's antibodies and HIV antigen. The objective of this study was to evaluate the clinical sensitivity and specificity of the new DiaSorin Liais...

journal_title：Journal of clinical microbiology

authors： Lemee V,Leoz M,Etienne M,De Oliveira F,Plantier JC

更新日期：2014-09-01 00:00:00

abstract：:Staphylococcus epidermidis, a human commensal, is a common cause of bacteremia in immunocompromised patients with indwelling medical devices. We report a case of isolated cervical adenitis caused by S. epidermidis in an immunocompetent patient and comment on the presumed pathogenesis. ...

journal_title：Journal of clinical microbiology

authors： Ryan-Poirier K,Patrick CC

更新日期：1993-02-01 00:00:00

abstract：:A toxigenic Corynebacterium diphtheriae biovar mitis sequence type 136 (ST136) strain was recovered from a toe infection of an unvaccinated patient recently returned from India. The isolate was resistant to clindamycin, erythromycin (ermX positive), tetracycline, and trimethoprim-sulfamethoxazole, intermediate to ceft...

journal_title：Journal of clinical microbiology

authors： Mina NV,Burdz T,Wiebe D,Rai JS,Rahim T,Shing F,Hoang L,Bernard K

更新日期：2011-11-01 00:00:00