Evaluation of PNA-FISH yeast traffic light for rapid identification of yeast directly from positive blood cultures and assessment of clinical impact.

abstract：:Anaerobes are an important but uncommon cause of bloodstream infections (BSIs). For pediatric patients, routine inclusion of an anaerobic blood culture alongside the aerobic remains controversial. We implemented automatic anaerobic blood culture alongside aerobic blood cultures in a pediatric emergency department (ED)...

journal_title：Journal of clinical microbiology

authors： Dien Bard J,Chang TP,Yee R,Manshadi K,Lichtenfeld N,Choi HJ,Festekjian A

更新日期：2020-08-24 00:00:00

abstract：:Todd-Hewitt broth (THB) containing colistin and nalidixic acid was compared with four other media with respect to efficacy of isolation of group B streptocci. It was effective as plain THB, THB with gentamicin and nalidixic acid and blood, and THB with colistin, nalidixic acid, and blood. THB with gentamicin and nalid...

journal_title：Journal of clinical microbiology

authors： Fenton LJ,Harper MH

更新日期：1979-02-01 00:00:00

abstract：:Several problems remain before molecular biology-based techniques, such as PCR, are widely accepted for the detection of infectious agents. Among the most formidable of these problems are the inability of the tests to distinguish between viable and nonviable organisms. We approached this problem by using the fact that...

journal_title：Journal of clinical microbiology

authors： Jou NT,Yoshimori RB,Mason GR,Louie JS,Liebling MR

更新日期：1997-05-01 00:00:00

abstract：:A rapid dot blot hybridization assay for the detection of B19 parvovirus DNA in human sera was developed. Small portions of four serum samples were mixed, filtered onto a nylon membrane, and hybridized with a digoxigenin-labeled DNA probe; for each membrane, 380 serum samples could be tested. When a dot was positive b...

journal_title：Journal of clinical microbiology

authors： Zerbini M,Musiani M,Venturoli S,Gallinella G,Gibellini D,Gentilomi G,La Placa M

更新日期：1990-11-01 00:00:00

abstract：:A new PCR-reverse dot blot hybridization (RDBH) assay was developed for the rapid identification of Mycobacterium species in clinical isolates. The assay, which targets the 16S rRNA, was evaluated for 27 mycobacterial reference strains and 340 clinical isolates that were simultaneously identified by DNA sequencing and...

journal_title：Journal of clinical microbiology

authors： Wu X,Zhang J,Liang J,Lu Y,Li H,Li C,Yue J,Zhang L,Liu Z

更新日期：2007-06-01 00:00:00

abstract：:Mycobacterium tuberculosis isolates were studied by comparing chromosomal DNA fingerprint patterns produced by digestion of chromosomal DNA with BamHI, followed by agarose electrophoresis and hybridization with radiolabeled probes of insertion sequence IS6110. DNA fingerprints of 14 isolates from separate members of f...

journal_title：Journal of clinical microbiology

authors： Mazurek GH,Cave MD,Eisenach KD,Wallace RJ Jr,Bates JH,Crawford JT

更新日期：1991-09-01 00:00:00

abstract：:Invasive pulmonary aspergillosis (IPA) is a frequently fatal infection in immunocompromised patients that is difficult to diagnose. Present methods for detection of Aspergillus spp. in bronchoalveolar lavage (BAL) fluid and in tissue vary in sensitivity and specificity. We therefore developed an A. fumigatus-specific ...

journal_title：Journal of clinical microbiology

authors： O'Sullivan CE,Kasai M,Francesconi A,Petraitis V,Petraitiene R,Kelaher AM,Sarafandi AA,Walsh TJ

更新日期：2003-12-01 00:00:00

abstract：:We report a case of bacteremia caused by Janibacter melonis, a recently described aerobic actinomycete originally isolated from a spoiled oriental melon. Our patient's blood culture isolate was identified by partial 16S rRNA gene sequencing. This is the first report of the recovery of Janibacter species from humans. ...

journal_title：Journal of clinical microbiology

authors： Elsayed S,Zhang K

更新日期：2005-07-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) resistance to antivirals is a major problem in immunocompromised patients. Drug resistance is characterized by phenotypic testing or genotypic analysis of the phosphotransferase (UL97) and DNA polymerase (UL54) genes. However, genotypic assays require further characterization of unknown mu...

journal_title：Journal of clinical microbiology

authors： Drouot E,Piret J,Boivin G

更新日期：2013-10-01 00:00:00

abstract：:Escherichia hermannii was isolated in pure culture from a patient with acute purulent conjunctivitis after a minor ocular injury. This is the first report of E. hermannii isolated as the sole pathogen from an infected site without prior antibiotic exposure, confirming the pathogenic potential of the microorganism. ...

journal_title：Journal of clinical microbiology

authors： Poulou A,Dimitroulia E,Markou F,Tsakris A

更新日期：2008-11-01 00:00:00

abstract：:The diagnosis of Clostridium difficile infection continues to be a challenge for many clinical microbiology laboratories. A new lateral flow assay, the C.Diff Quik Chek Complete assay, which tests for the presence of both glutamate dehydrogenase (GDH) and C. difficile toxins A and B, was evaluated for its ability to d...

journal_title：Journal of clinical microbiology

authors： Sharp SE,Ruden LO,Pohl JC,Hatcher PA,Jayne LM,Ivie WM

更新日期：2010-06-01 00:00:00

abstract：:We investigated a method to reduce oxygen tension and produce anaerobic conditions conveniently by coculture with a nonfermentative organism. The biochemical reactions of 94 strains representing 41 different species were determined using prereduced media and gas chromatography according to standard methods. The standa...

journal_title：Journal of clinical microbiology

authors： Hussain Z,Bürger H,Lannigan R,Groves DJ

更新日期：1984-02-01 00:00:00

abstract：:Clostridium difficile is the leading cause of antibiotic-associated diarrhea and pseudomembranous colitis, which have significant morbidity and mortality. Accurate and timely diagnosis is critical. Repeat enzyme immunoassay testing for C. difficile toxin has been recommended because of <100% sensitivity. All C. diffic...

journal_title：Journal of clinical microbiology

authors： Cardona DM,Rand KH

更新日期：2008-11-01 00:00:00

abstract：:Rapid and accurate tests are currently needed to identify individuals with high levels of Loa loa microfilaria (mf), so that these individuals may be excluded from mass ivermectin administration campaigns against onchocerciasis and lymphatic filariasis being conducted in areas where Onchocerca volvulus, Wuchereria ban...

journal_title：Journal of clinical microbiology

authors： Drame PM,Fink DL,Kamgno J,Herrick JA,Nutman TB

更新日期：2014-06-01 00:00:00

abstract：:This case report describes the fungal dermatitis caused by Neosartorya hiratsukae infection in a household hedgehog confirmed by microscopic examination of conidiophores and DNA analyses including the internal transcribed spacer region, partial beta-tubulin, and the calmodulin gene. It is the first report of a natural...

journal_title：Journal of clinical microbiology

authors： Han JI,Na KJ

更新日期：2008-09-01 00:00:00

abstract：:Longitudinal data on the E6/E7 mRNA-based Aptima human papillomavirus (AHPV) assay exceeding three years in comparison to the gold standard Digene Hybrid Capture 2 (HC2) test are not available. We previously reported the cross-sectional data of the German AHPV Screening Trial (GAST) in which 10,040 women were recruite...

journal_title：Journal of clinical microbiology

authors： Iftner T,Neis KJ,Castanon A,Landy R,Holz B,Woll-Herrmann A,Iftner A,Staebler A,Wallwiener D,Hann von Weyhern C,Neis F,Haedicke-Jarboui J,Martus P,Brucker S,Henes M,Sasieni P

更新日期：2019-01-02 00:00:00

abstract：:During a 3-year period, all Pasteurella strains recovered at the Clinical Microbiological Laboratory, Lund, Sweden, were studied biochemically with respect to their relationship to the recently described taxa of this genus. Of 159 strains recovered from 146 infected humans, 95 were identified as Pasteurella multocida ...

journal_title：Journal of clinical microbiology

authors： Holst E,Rollof J,Larsson L,Nielsen JP

更新日期：1992-11-01 00:00:00

abstract：:The standardized disk diffusion test, in which a 10-micrograms enoxacin disk is used, was performed and microbroth dilution MICs were determined to establish individual test control values with Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, and S. aureus ATCC 29213. I...

journal_title：Journal of clinical microbiology

authors： Rudrik JT,Cavalieri SJ,Britt EM

更新日期：1985-03-01 00:00:00

abstract：:Real-time PCR methodology can be applied to rapidly and accurately detect influenza viruses. During times of surge testing or enhanced pandemic surveillance, public health laboratories (PHLs) may experience overwhelming demand for testing, even while the prevalence of positive specimens remains low. To improve laborat...

journal_title：Journal of clinical microbiology

authors： Van TT,Miller J,Warshauer DM,Reisdorf E,Jernigan D,Humes R,Shult PA

更新日期：2012-03-01 00:00:00

abstract：:Influenza viruses related to the markedly antigenically divergent strains B/Yamagata/16/88 and B/Victoria/2/87 are circulating in human populations. Adults develop cross-reacting antibodies against recent and earlier influenza B virus strains after vaccination with B/Yamagata/16/88, probably because of previous influe...

journal_title：Journal of clinical microbiology

authors： Levandowski RA,Gross PA,Weksler M,Staton E,Williams MS,Bonelli J

更新日期：1991-07-01 00:00:00

abstract：:A public health response relies upon rapid and reliable confirmation of disease by diagnostic assays. Here, we detail the design and validation of two variola virus-specific real-time PCR assays, since previous assays cross-reacted with newly identified cowpox viruses. The assay specificity must continually be reasses...

journal_title：Journal of clinical microbiology

authors： Kondas AV,Olson VA,Li Y,Abel J,Laker M,Rose L,Wilkins K,Turner J,Kline R,Damon IK

更新日期：2015-04-01 00:00:00

abstract：:Pseudobacteremias in blood cultures performed on the BACTEC radiometric blood culture system have been reported. We report three cases of cross contamination with Mycobacterium avium that occurred when mycobacteria were cultured with the BACTEC 460 TB system. Malfunction of the needle sterilization heating block was d...

journal_title：Journal of clinical microbiology

authors： Vannier AM,Tarrand JJ,Murray PR

更新日期：1988-09-01 00:00:00

abstract：:This study assesses human papillomavirus (HPV) detection and genotyping in self-sampled genital smears applied to an indicating FTA elute cartridge (FTA cartridge). The study group consisted of 96 women, divided into two sample sets. All samples were analyzed by the HPV SPF(10)-Line Blot 25. Set 1 consisted of 45 wome...

journal_title：Journal of clinical microbiology

authors： Lenselink CH,de Bie RP,van Hamont D,Bakkers JM,Quint WG,Massuger LF,Bekkers RL,Melchers WJ

更新日期：2009-08-01 00:00:00

abstract：:Clinical and experimental observations suggest that immune globulin may prevent otitis media (OM) in children. We performed experiments in chinchillas to test the hypothesis that human bacterial polysaccharide immune globulin (BPIG) might prevent OM caused by Streptococcus pneumoniae. Animals were given BPIG or saline...

journal_title：Journal of clinical microbiology

authors： Shurin PA,Giebink GS,Wegman DL,Ambrosino D,Rholl J,Overman M,Bauer T,Siber GR

更新日期：1988-04-01 00:00:00

abstract：:An ATP-based biocide susceptibility assay for mycobacteria was developed by optimizing the cell lysis and assay conditions. Compared to the conventional agar plating method, the assay was rapid (1.5 h) and showed high sensitivity and specificity as determined by receiver operating characteristic (ROC) analysis. The te...

journal_title：Journal of clinical microbiology

authors： Kapoor R,Yadav JS

更新日期：2010-10-01 00:00:00

abstract：:The genetic diversity of 123 Streptococcus suis strains of capsular types 2, 1/2, 3, 7, and 9, isolated from pigs in France and from humans in different countries, was evaluated by pulsed-field gel electrophoresis (PFGE) of DNA restricted with SmaI. The method was highly discriminative (D = 0.98), results were reprodu...

journal_title：Journal of clinical microbiology

authors： Berthelot-Hérault F,Marois C,Gottschalk M,Kobisch M

更新日期：2002-02-01 00:00:00

abstract：:To determine the excretion dynamics and genotypic characteristics of rotavirus A (RVA), a longitudinal observational study was performed in 10 pigs from 3 litters at a farrow-to-finish farm. A total of 400 fecal samples were directly collected from the rectums of individual pigs (aged 7 to 217 days) at 3- to 14-day in...

journal_title：Journal of clinical microbiology

authors： Miyazaki A,Kuga K,Suzuki T,Tsunemitsu H

更新日期：2012-06-01 00:00:00

abstract：:Low-speed centrifugation-mediated adsorption was evaluated as an enhancement of infectivity of clinical and laboratory strains of cytomegalovirus (CMV) occurring with cells grown in conventional culture tubes. The time required for reporting of primary isolates of CMV from urine specimens adsorbed onto monolayers of W...

journal_title：Journal of clinical microbiology

authors： Oefinger PE,Shawar RM,Loo SH,Tsai LT,Arnett JK

更新日期：1990-05-01 00:00:00

abstract：:Enterococcus hirae is a rare isolate in clinical specimens. We describe a case of native aortic-valve endocarditis that was caused by Enterococcus hirae in a 72-year-old man. This is the first reported case of endocarditis due to this organism. ...

journal_title：Journal of clinical microbiology

authors： Poyart C,Lambert T,Morand P,Abassade P,Quesne G,Baudouy Y,Trieu-Cuot P

更新日期：2002-07-01 00:00:00

abstract：:A modified indirect hemagglutination test which enabled Pasteurella haemolytica to be serotyped in 3 h is described. There was 100% correlation with the conventional test for both the 15 prototype strains and the 50 field isolates. It was found that nonviable cultures could also be serotyped. ...

journal_title：Journal of clinical microbiology

authors： Fraser J,Donachie W,Quirie M,Gilmour NJ

更新日期：1983-07-01 00:00:00