Abstract:
:First-void urine (FVU) is the preferred specimen for the diagnosis of urogenital Chlamydia trachomatis infection in men. We have developed FirstBurst, a urine collection device that collects the first 4 to 5 ml of FVU and yields a specimen with a sixfold higher C. trachomatis organism load than the regular urine cup by quantitative PCR (32,533 versus 5,271 plasmids/ml; P < 0.0001). Consequently, the use of FirstBurst to collect a urine sample improved the sensitivity of a rapid test for Chlamydia over testing of samples collected with a urine cup (82 versus 47% sensitivity using PCR as a reference; P < 0.0015).
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Wisniewski CA,White JA,Michel CE,Mahilum-Tapay L,Magbanua JP,Nadala EC Jr,Barber PJ,Goh BT,Lee HHdoi
10.1128/JCM.02241-07subject
Has Abstractpub_date
2008-04-01 00:00:00pages
1466-9issue
4eissn
0095-1137issn
1098-660Xpii
JCM.02241-07journal_volume
46pub_type
杂志文章abstract::This is the first reported case of native valve endocarditis caused by Engyodontium album. This fungus, rarely seen as a human pathogen, is separated from Tritirachium species by its lack of pigmentation and from Beauveria species by the presence of conidiogenous cells in whorls. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.6.1479-1481.1990
更新日期:1990-06-01 00:00:00
abstract::Whole chromosomal DNA probes were used to identify clinical isolates of Mycobacterium tuberculosis, Mycobacterium avium complex, and Mycobacterium gordonae. The probe for M. tuberculosis was prepared from Mycobacterium bovis BCG, which has been shown to be closely related to M. tuberculosis. A probe for the M. avium c...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.7.1239-1243.1987
更新日期:1987-07-01 00:00:00
abstract::Levels of hepatitis B virus (HBV) DNA in the blood serve as an important marker in monitoring the disease progression and treatment efficacy of chronic HBV infection. Several commercial assays are available for accurate measurement of HBV genomic DNA, but many of them are hampered by relatively low sensitivity and lim...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.8.3438-3440.2004
更新日期:2004-08-01 00:00:00
abstract::Acinetobacter isolates associated with casualties from the Iraq conflict from the United States were compared with those from the United Kingdom by pulsed-field gel electrophoresis and integron analysis. Representatives of the main outbreak strain associated with casualties from both countries were indistinguishable i...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00547-06
更新日期:2006-07-01 00:00:00
abstract::Gemella morbillorum and Gemella haemolysans are opportunistic pathogens which cause endocarditis and other severe infections. We report on three patients with endocarditis, one with endocarditis caused by G. haemolysans and two with endocarditis caused by G. morbillorum. The paucity of reports concerning these bacteri...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,评审
doi:10.1128/JCM.36.4.866-871.1998
更新日期:1998-04-01 00:00:00
abstract::A cross-sectional study of nasopharyngeal colonization with Streptococcus pneumoniae was performed among 573 children attending 29 day-care centers (DCCs) in Norway prior to the start of mass vaccination with the heptavalent pneumococcal conjugate vaccine (PCV-7). A sensitive sampling method was employed, including tr...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02296-07
更新日期:2008-08-01 00:00:00
abstract::Streptococcus agalactiae causes severe invasive disease in humans and mastitis in cattle. Temporally matched bovine milk isolates and clinical human invasive isolates (52 each) collected in New York State over 18 months were characterized by molecular subtyping and phenotypic methods to probe the interspecies transmis...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.3.1177-1186.2005
更新日期:2005-03-01 00:00:00
abstract::A serotyping scheme for Enterobacter cloacae based on heat-stable somatic antigens is described. A total of 28 antisera were prepared in rabbits, and titers of agglutinins were high (greater than 640). Some cross-reactions were observed, and 11 sera required absorption before routine use. Of 300 clinical isolates from...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.18.5.1079-1083.1983
更新日期:1983-11-01 00:00:00
abstract::Replica plating can be used for the detection of antibiotic resistance in normal flora. We have evaluated this application of the replica plating method by comparing it with a five-colony method. The replica plating method uses a single plate for each antibiotic, with a concentration just above that for borderline res...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.33.12.3146-3149.1995
更新日期:1995-12-01 00:00:00
abstract::A total of 309 Helicobacter pylori isolates from 18 different countries were analyzed with a previously developed lectin typing system. The system was developed by using a proteolytic pretreatment to enhance the carbohydrate fraction of the sample. Four lectins from Ulex europaeus, Lotus tetragonolobus, Erythrina cris...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.40.1.227-232.2002
更新日期:2002-01-01 00:00:00
abstract::In a prospective observational study of bacteremic patients we ascertained the influence of different parts of culture results on the correctness of empirical antibiotic therapy. Ninety-three bacteremic patients requiring antibiotic treatment were included. Patients who had consultations with an infectious disease con...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.06051-11
更新日期:2012-06-01 00:00:00
abstract::Treatment of patients with chronic hepatitis B virus (HBV) infections with potent antiviral therapy often results in dramatic reductions in the levels of viremia to very low levels. Monitoring of serum HBV DNA levels is a consistent method for the assessment of antiviral potency; however, widely used hybridization ass...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.40.6.1972-1976.2002
更新日期:2002-06-01 00:00:00
abstract::A Campylobacter-like organism was isolated from the ilea of normal hamsters. The organism was isolated from an ileal homogenate which was passed through a filter (0.65-micron pore size) and cultured on blood-agar plates in a microaerophilic atmosphere at 37 degrees C. Pinpoint translucent colonies were first observed ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.27.11.2497-2501.1989
更新日期:1989-11-01 00:00:00
abstract::Bacteriuria due to Salmonella typhi usually occurs following recent typhoid fever or in chronic carrier states. Data from 18 patients with S. typhi bacteriuria, seen during 5 years, were analyzed. Fourteen patients had localized urinary tract infection due to S. typhi. Four others had bacteriuria, probably associated ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.33.7.1791-1792.1995
更新日期:1995-07-01 00:00:00
abstract::Fourth-generation human immunodeficiency virus (HIV) screening immunoassays reduce the diagnostic window between infection and diagnosis by the inclusion of HIV p24 antigen detection together with HIV antibody detection in the same test. We compared third- and fourth-generation HIV immunoassays and a dedicated HIV p24...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.10.5397-5399.2005
更新日期:2005-10-01 00:00:00
abstract::Enteroaggregative Escherichia coli (EAEC) is an emerging enteric pathogen that causes acute and chronic diarrhea among children, human immunodeficiency virus-infected patients, and travelers to developing regions of the world. The pathogenesis of EAEC strains involves the production of biofilm. In this study, we deter...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01128-06
更新日期:2007-01-01 00:00:00
abstract::Clostridium difficile is the bacterium most commonly surmised to cause antimicrobial- and hospital-associated diarrhea in developed countries worldwide, and such infections are thought to be increasing in frequency and severity. A laboratory-based study was carried out to characterize C. difficile strains isolated fro...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02437-07
更新日期:2008-09-01 00:00:00
abstract::Isolation of Burkholderia pseudomallei currently relies on the use of Ashdown's selective agar (ASA). We designed a new selective agar (Burkholderia pseudomallei selective agar [BPSA]) to improve recovery of the more easily inhibited strains of B. pseudomallei. B. pseudomallei, Burkholderia cepacia, and Pseudomonas ae...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.7.3312-3316.2003
更新日期:2003-07-01 00:00:00
abstract::To identify Trichinella antigens suitable for high-specificity and high-sensitivity serodiagnosis of human trichinellosis, we evaluated assays using four antigens: (i) crude first-stage larval extract (CLE), (ii) O-deglycosylated CLE, (iii) tyvelose-bearing antigens (Trichinella spiralis larva group 1 [TSL-1] antigens...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.9.4060-4066.2004
更新日期:2004-09-01 00:00:00
abstract::Seven rotavirus strains dually reactive to serotype G1- and G2-specific monoclonal antibodies (MAbs) from hospitalized children with rotavirus diarrhea were culture adapted. Six strains were neutralized with G1 antiserum to a higher titer than that of G2, and one was neutralized with G1 and G2 antisera to equal titers...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.11.5267-5269.2003
更新日期:2003-11-01 00:00:00
abstract::The heterogeneity and the increasing clinical importance of the Enterobacter cloacae complex have often been discussed. However, little is known about molecular factors causing pathogenicity within this nomenspecies. Here, we analyzed the genetic differences between an avirulent plant isolate and a pathogenic strain c...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00885-12
更新日期:2012-10-01 00:00:00
abstract::Between January 1988 and December 1989 Aeromonas species were isolated from 45 (1.8%) of 2,480 patients with acute gastroenteritis. No other bacterial enteric pathogens were found in any of these 45 patients. Of the 45 Aeromonas isolates, 35 strains (77.8%) were Aeromonas hydrophila, 7 (15.5%) were Aeromonas sobria, a...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.29.5.853-856.1991
更新日期:1991-05-01 00:00:00
abstract::The recent emergence of the multidrug-resistant and pathogenic yeast Candida auris continues to cause public health concern worldwide. C. auris is alarming because it causes health care-associated outbreaks and can establish invasive infections with high mortality rates. Transmission between patients is facilitated by...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01337-18
更新日期:2018-11-27 00:00:00
abstract::Gram-negative, non-saccharolytic, brown- or black-pigment-forming, nonmotile anaerboic coccobacilli, capable of decomposing hydrogen peroxide and identified as Bacteroides asaccharolyticus (B. melaninogenicus subsp. asaccharolyticus), were isolated from the supra- and subgingival plaques of beagle dogs with gingivitis...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.11.5.522-526.1980
更新日期:1980-05-01 00:00:00
abstract::Molecular techniques are playing an important role in the diagnosis of nontuberculous mycobacterial infections. This case report describes a chronic soft tissue infection in an immunocompetent patient caused by a previously undescribed pigmented, rapidly growing Mycobacterium species, emphasizing the importance of cli...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.6.2779-2782.2003
更新日期:2003-06-01 00:00:00
abstract::We describe the first case of Epstein-Barr virus (EBV)-associated thymic carcinoid tumor found by in situ hybridization (ISH) on paraffin-embedded sections. ISH revealed that both tumor cells and infiltrated lymphocytes were EBV positive, while a few EBV-infected lymphocytes were detected in 2 of 11 thymuses and 1 of ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.6.2850-2854.2004
更新日期:2004-06-01 00:00:00
abstract::We have developed a high-resolution melting (HRM) assay to scan for mutations in the rpoB, inhA, ahpC, and katG genes and/or promoter regions for the detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis. For assay development, 23 drug-resistant isolates of M. tuberculosis having 29 different mu...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02036-09
更新日期:2010-04-01 00:00:00
abstract::A European multicenter study was performed to evaluate the performance of a new method, based on the transcription-reverse transcription concerted reaction (TRC-2), which enabled one-step amplification and real-time detection of the Mycobacterium tuberculosis 16S rRNA target directly in clinical specimens. A total of ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
doi:10.1128/JCM.01730-08
更新日期:2009-11-01 00:00:00
abstract::The HERPCHEK herpes simplex virus (HSV) antigen test (Du Pont, Billerica, Mass.) is a biotinstreptavidin-amplified enzyme-linked immunoassay for the detection of HSV antigen directly in clinical specimens. In a total of 200 mucocutaneous specimens, predominantly lesions from nongenital sites, HERPCHEK had a sensitivit...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.27.8.1903-1905.1989
更新日期:1989-08-01 00:00:00
abstract::An association of enterovirus infection with endemic cardiomyopathy (Keshan disease [KD]) and outbreaks of myocarditis in selenium-deficient rural areas of southwestern China has been established. Enteroviruses have been isolated from patients with KD or during outbreaks of myocarditis in last two decades. Six of thes...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.38.10.3538-3543.2000
更新日期:2000-10-01 00:00:00