Francisella novicida bacteremia after a near-drowning accident.

abstract：:Sepsis is a major cause of death worldwide among nonhospitalized people and hospitalized patients. A wide range of pathogens are involved, and the correct identification and correct antimicrobial therapy are critical to ensure optimal clinical outcomes. With the recent introduction of matrix-assisted laser desorption ...

journal_title：Journal of clinical microbiology

authors： Foster AG

更新日期：2013-11-01 00:00:00

abstract：:Large hospital-based clinical laboratories must be prepared to rapidly investigate potential infectious disease outbreaks. To challenge the ability of our molecular diagnostics laboratory to use whole-genome sequencing in a potential outbreak scenario and identify impediments to these efforts, we studied 84 invasive s...

journal_title：Journal of clinical microbiology

authors： Olsen RJ,Fittipaldi N,Kachroo P,Sanson MA,Long SW,Como-Sabetti KJ,Valson C,Cantu C,Lynfield R,Van Beneden C,Beres SB,Musser JM

更新日期：2014-12-01 00:00:00

abstract：:Neisseria meningitidis is the causative agent of meningococcal sepsis and meningitis. Neisseria polysaccharea is a nonpathogenic species. N. polysaccharea is able to use sucrose to produce amylopectin, a starch-like polysaccharide, which distinguishes it biochemically from the pathogenic species N. meningitidis. The d...

journal_title：Journal of clinical microbiology

authors： Zhu P,Tsang RS,Tsai CM

更新日期：2003-01-01 00:00:00

abstract：:Salmonella typhi was isolated from 369 and Salmonella paratyphi A was isolated from 6 of 515 Vietnamese patients with suspected enteric fever. Compared with conventional broth culture of blood, direct plating of the buffy coat had a diagnostic sensitivity of 99.5% (95% confidence interval [CI], 97.1 to 100%). Blood ba...

journal_title：Journal of clinical microbiology

authors： Wain J,Diep TS,Ho VA,Walsh AM,Nguyen TT,Parry CM,White NJ

更新日期：1998-06-01 00:00:00

abstract：:A new compound, indoxyl-beta-D-glucuronide, was assessed as a substrate for the rapid detection of Escherichia coli in urine. Incorporation of this compound into MacConkey agar allowed the direct differentiation of E. coli as deep blue colonies distinct from lactose and nonlactose fermenters. The sensitivity was 88 to...

journal_title：Journal of clinical microbiology

authors： Delisle GJ,Ley A

更新日期：1989-04-01 00:00:00

abstract：:Participants in the College of American Pathologists' Mycobacteriology E proficiency testing survey in 1993 were asked to complete a questionnaire addressing mycobacterial test methods, test volume, and frequency of detection of drug-resistant Mycobacterium tuberculosis (MTB). A similar questionnaire had been distribu...

journal_title：Journal of clinical microbiology

authors： Woods GL,Witebsky FG

更新日期：1995-02-01 00:00:00

abstract：:Microsporidia are protozoa parasites responsible for significant gastrointestinal disease in patients infected with human immunodeficiency virus. We evaluated a PCR assay of stool samples, duodenal aspirates, and biopsy specimens from patients with Enterocytozoon bieneusi infection. A 210-bp DNA fragment of the unique...

journal_title：Journal of clinical microbiology

authors： Velásquez JN,Carnevale S,Guarnera EA,Labbé JH,Chertcoff A,Cabrera MG,Rodríguez MI

更新日期：1996-12-01 00:00:00

abstract：:Sequencing of the Mycobacterium tuberculosis pncA gene allows for pyrazinamide susceptibility testing. We summarize data on pncA polymorphisms that do not confer resistance at a susceptibility breakpoint of 100 μg/ml pyrazinamide in MGIT within a cohort of isolates from South Africa and the U.S. Centers for Disease Co...

journal_title：Journal of clinical microbiology

authors： Whitfield MG,Warren RM,Streicher EM,Sampson SL,Sirgel FA,van Helden PD,Mercante A,Willby M,Hughes K,Birkness K,Morlock G,van Rie A,Posey JE

更新日期：2015-11-01 00:00:00

abstract：:Rapid tests for glucose, maltose, lactose, sucrose, and starch fermentation were performed on 112 strains of anaerobic bacteria. The tests were incubated under aerobic conditions, and results were read within 4 h. An overall correlation of 89% was achieved between the rapid tests and the Virginia Polytechnic Institut...

journal_title：Journal of clinical microbiology

authors： Schreckenberger PC,Blazevic DJ

更新日期：1976-03-01 00:00:00

abstract：:Tracking the spread of noroviruses during outbreaks of gastroenteritis is hampered by the lack of sequence diversity in those regions of the genome chosen for virus detection and characterization. Sequence analysis of regions of the genes encoding the RNA-dependent RNA polymerase and the S domain of the capsid does no...

journal_title：Journal of clinical microbiology

authors： Xerry J,Gallimore CI,Iturriza-Gómara M,Allen DJ,Gray JJ

更新日期：2008-03-01 00:00:00

abstract：:DNA restriction analysis was carried out on a sample of 212 epidemiologically unrelated adenovirus (Ad) strains of serotype 7 isolated in Chile, Uruguay, and Argentina from nasopharyngeal aspirates of children with acute lower respiratory disease between 1984 and 1990. The identified genome types were Ad7b (n = 12), A...

journal_title：Journal of clinical microbiology

authors： Kajon A,Wadell G

更新日期：1994-09-01 00:00:00

abstract：:Phenotypic, genotypic, and toxin gene analyses have not yet been done all in one for the Nigerian Staphylococcus aureus population. This study provides a comprehensive overview of the molecular epidemiology and genetic diversity of S. aureus strains at the largest university clinic in Ibadan, Nigeria. From 1,300 patie...

journal_title：Journal of clinical microbiology

authors： Ghebremedhin B,Olugbosi MO,Raji AM,Layer F,Bakare RA,König B,König W

更新日期：2009-09-01 00:00:00

abstract：:A micro enzyme-linked immunosorbent assay was used to detect the presence of amoebic antigen in polyethylene-glycol-precipitated circulating immune complexes (CIC). A cutoff dilution of greater than 1:160 for the precipitates was taken to be of clinical significance. Among the patients with amoebic liver abscess, 93% ...

journal_title：Journal of clinical microbiology

authors： Vinayak VK,Purnima,Singh K,Venkatwswarlu K,Nain CK,Mehta SK

更新日期：1986-06-01 00:00:00

abstract：:Anthopsis deltoidea was found to be the cause of an olecranon bursitis in a 79-year-old golfer. Serial histological sections of the olecranon bursa showed faintly stained, brown-walled, septate, hyphal elements in the centers of the necrotic debris. The combination of bursectomy and flucytosine treatment cured the inf...

journal_title：Journal of clinical microbiology

authors： Kwon-Chung KJ,Droller DD

更新日期：1984-08-01 00:00:00

abstract：:A differential staining method employing acridine orange-stained rickettsiae and safranin-stained standardized suspensions of Shigella dysenteriae is described for enumerating rickettsiae harvested during various stages of the growth cycle. ...

journal_title：Journal of clinical microbiology

authors： Silverman DJ,Fiset P,Wisseman CL Jr

更新日期：1979-03-01 00:00:00

abstract：:Amplification of human papillomavirus (HPV) DNA by L1 consensus primer systems (e.g., MY09/11 or GP5(+)/6(+)) can detect as few as 10 to 100 molecules of HPV targets from a genital sample. However, genotype determination by dot blot hybridization is laborious and requires at least 27 separate hybridizations for substa...

journal_title：Journal of clinical microbiology

authors： Gravitt PE,Peyton CL,Apple RJ,Wheeler CM

更新日期：1998-10-01 00:00:00

abstract：:We evaluated a newly commercial enzyme immunoassay (EIA) (Biotest Legionella Urin Antigen EIA; Biotest AG, Dreieich, Germany) for detection of antigens of all Legionella pneumophila serogroups with a relatively wide spectrum of cross-reactivity as well as antigens of other Legionella spp. by comparing its sensitivity ...

journal_title：Journal of clinical microbiology

authors： Domínguez JA,Galí N,Pedroso P,Fargas A,Padilla E,Manterola JM,Matas L

更新日期：1998-09-01 00:00:00

abstract：:A sandwich enzyme-linked immunosorbent assay was developed for its potential utility in the detection of antigen in the cerebrospinal fluid of patients with tuberculous meningitis. Cerebrospinal fluids examined included those from untreated (group Ia) and treated (group Ib) Mycobacterium tuberculosis meningitis, nonse...

journal_title：Journal of clinical microbiology

authors： Kadival GV,Mazarelo TB,Chaparas SD

更新日期：1986-05-01 00:00:00

abstract：:This study involved the rapid, direct identification of Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, Haemophilus influenzae, Enterococcus sp., and Streptococcus agalactiae from positive blood culture bottles (BACTEC, Johnston Laboratories, Inc.) by using the AccuProbe (Gen-Probe, San Diego, Calif...

journal_title：Journal of clinical microbiology

authors： Davis TE,Fuller DD

更新日期：1991-10-01 00:00:00

abstract：:Vulvovaginal candidiasis (VVC) remains a common cause of morbidity, with three-quarters of women affected during their lifetimes. Use of antibiotics is an acknowledged trigger for VVC, which adversely affects women's physical and emotional health. Knowledge of patterns of genital Candida species-level identification i...

journal_title：Journal of clinical microbiology

authors： Pirotta MV,Garland SM

更新日期：2006-09-01 00:00:00

abstract：:Ectromelia virus, an orthopoxvirus that can cause extensive morbidity and mortality (mousepox) in colonized mice, has been epizootically responsible for serious disruption of biomedical research since 1930. The lack of a sensitive and specific serological assay for infection with this virus became apparent during outb...

journal_title：Journal of clinical microbiology

authors： Buller RM,Bhatt PN,Wallace GD

更新日期：1983-11-01 00:00:00

abstract：:Streptococcus pneumoniae is an important cause of community-acquired pneumonia. However, in this setting the diagnostic sensitivity of blood cultures is below 30%. Since during such infections changes in the amounts of S. pneumoniae may also occur in the upper respiratory tract, quantification of these bacteria in nas...

journal_title：Journal of clinical microbiology

authors： Greiner O,Day PJ,Bosshard PP,Imeri F,Altwegg M,Nadal D

更新日期：2001-09-01 00:00:00

abstract：:We compared culture results to investigate the influence of antimicrobial-loaded cement on sonicate fluid culture positivity for the diagnosis of prosthetic joint infection. Fifty-four subjects were assessed. The sensitivities of sonicate fluid culture were 77.8% (14 of 18) in subjects with an antimicrobial-loaded cem...

journal_title：Journal of clinical microbiology

authors： Park KH,Greenwood-Quaintance KE,Hanssen AD,Abdel MP,Patel R

更新日期：2016-06-01 00:00:00

abstract：:The test approved by the U.S. Food and Drug Administration for assessment of the barrier quality of medical exam gloves includes visual inspection and a water leak test. Neither method tests directly the ability of gloves to prevent penetration by microorganisms. Methods that use microorganisms (viruses and bacteria) ...

journal_title：Journal of clinical microbiology

authors： Broyles JM,O'Connell KP,Korniewicz DM

更新日期：2002-08-01 00:00:00

abstract：:Droplet digital PCR (ddPCR) is an emulsion PCR process that performs absolute quantitation of nucleic acids. We developed a ddPCR assay for Chlamydia trachomatis infections and found it to be accurate and precise. Using PCR mixtures containing plasmids engineered to include the PCR target sequences, we were able to qu...

journal_title：Journal of clinical microbiology

authors： Roberts CH,Last A,Molina-Gonzalez S,Cassama E,Butcher R,Nabicassa M,McCarthy E,Burr SE,Mabey DC,Bailey RL,Holland MJ

更新日期：2013-07-01 00:00:00

abstract：:Although screening for maternal toxoplasmic seroconversion during pregnancy is based on immunodiagnostic assays, the diagnosis of clinically relevant toxoplasmosis greatly relies upon molecular methods. A problem is that this molecular diagnosis is subject to variation of performances, mainly due to a large diversity ...

journal_title：Journal of clinical microbiology

authors： Sterkers Y,Varlet-Marie E,Cassaing S,Brenier-Pinchart MP,Brun S,Dalle F,Delhaes L,Filisetti D,Pelloux H,Yera H,Bastien P

更新日期：2010-09-01 00:00:00

abstract：:Antibody to equid herpesvirus 1, which mediates single radial hemolysis, is that responsible for neutralization. Hemagglutination inhibition antibody is not necessarily involved in neutralization or hemolysis. ...

journal_title：Journal of clinical microbiology

authors： Klingeborn B,Dinter Z

更新日期：1978-05-01 00:00:00

abstract：:Two hundred seventy nasopharyngeal aspirates were tested in duplicate with the Ortho Diagnostics, Inc. (Raritan, N.J.), respiratory syncytial virus antigen enzyme-linked immunosorbent assay. The test was sensitive (80 to 82%) and specific (96%) when compared with cell culture. The enzyme-linked immunosorbent assay det...

journal_title：Journal of clinical microbiology

authors： Bromberg K,Tannis G,Daidone B,Clarke L,Sierra MF

更新日期：1985-12-01 00:00:00

abstract：:Several methods have been previously suggested for the presumptive identification of Gardnerella vaginalis in clinical laboratories, but none is entirely satisfactory. We previously found that sodium polyanetholesulfonate (SPS) inhibits G. vaginalis in blood culture media. We compared susceptibility to an SPS-containi...

journal_title：Journal of clinical microbiology

authors： Reimer LG,Reller LB

更新日期：1985-02-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) resistance to antivirals is a major problem in immunocompromised patients. Drug resistance is characterized by phenotypic testing or genotypic analysis of the phosphotransferase (UL97) and DNA polymerase (UL54) genes. However, genotypic assays require further characterization of unknown mu...

journal_title：Journal of clinical microbiology

authors： Drouot E,Piret J,Boivin G

更新日期：2013-10-01 00:00:00