Abstract:
:The test approved by the U.S. Food and Drug Administration for assessment of the barrier quality of medical exam gloves includes visual inspection and a water leak test. Neither method tests directly the ability of gloves to prevent penetration by microorganisms. Methods that use microorganisms (viruses and bacteria) to test gloves have been developed but require classical culturing of the organism to detect it. We have developed a PCR assay for bacteriophage phiX174 that allows the rapid detection of penetration of gloves by this virus. The method is suitable for use with both latex and synthetic gloves. The presence of glove powder on either latex or synthetic gloves had no effect on the ability of the PCR assay to detect bacteriophage DNA. The assay is rapid, sensitive, and inexpensive; requires only small sample volumes; and can be automated.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Broyles JM,O'Connell KP,Korniewicz DMdoi
10.1128/jcm.40.8.2725-2728.2002subject
Has Abstractpub_date
2002-08-01 00:00:00pages
2725-8issue
8eissn
0095-1137issn
1098-660Xjournal_volume
40pub_type
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.19.3.338-341.1984
更新日期:1984-03-01 00:00:00
abstract::Class-specific enzyme-linked immunosorbent assays (ELISAs) with purified recombinant antigens of Borrelia burgdorferi sensu stricto and Western blot analyses with whole cells of this spirochete were used to test human sera to determine which antigens were diagnostically important. In analyses for immunoglobulin M (IgM...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.38.5.1735-1739.2000
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.9.1867-1868.1988
更新日期:1988-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-09-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 临床试验,杂志文章
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更新日期:1988-09-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:1986-02-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2002-10-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.3.525-529.1990
更新日期:1990-03-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.11.3056-3057.1993
更新日期:1993-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JCM.43.5.2380-2383.2005
更新日期:2005-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jcm.41.8.3964-3966.2003
更新日期:2003-08-01 00:00:00
abstract::A reverse transcription-seminested PCR (RT-snPCR) assay was developed for the detection and identification of enterovirus (EV) RNA in clinical specimens. Three conserved protein motifs were identified by aligning the VP3 and VP1 sequences of prototype EV strains. Consensus degenerate primers were designed from a conse...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00542-06
更新日期:2006-08-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.10.2648-2653.1993
更新日期:1993-10-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.7.1239-1243.1987
更新日期:1987-07-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.20.6.1049-1052.1984
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
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doi:10.1128/JCM.37.11.3774-3777.1999
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.21.4.622-625.1985
更新日期:1985-04-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.9.2158-2161.1994
更新日期:1994-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JCM.18.5.1252-1255.1983
更新日期:1983-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-05-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2018-02-22 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.12.6126-6129.2005
更新日期:2005-12-01 00:00:00
abstract::We attempted to find a relationship between the microbiological properties of bloodstream isolates of methicillin-resistant Staphylococcus aureus (MRSA) and the efficacy of vancomycin in the treatment of bacteremia. Vancomycin susceptibility testing was performed, and bactericidal activity was determined for 30 isolat...
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pub_type: 临床试验,杂志文章,多中心研究
doi:10.1128/JCM.42.6.2398-2402.2004
更新日期:2004-06-01 00:00:00