Comparison of antigen immunoassay and reverse transcriptase assay for monitoring human immunodeficiency virus infection in an antiviral trial.

abstract：:We compared a set of commercial Salmonella somatic and flagellar serotyping antisera to in-house-prepared antisera from the Microbial Diseases Laboratory, California Department of Public Health, using 327 Salmonella enterica strains belonging to subgroups I, II, IIIa, IIIb, and IV. The sensitivities of Denka Seiken (T...

journal_title：Journal of clinical microbiology

authors： Schrader KN,Fernandez-Castro A,Cheung WK,Crandall CM,Abbott SL

更新日期：2008-02-01 00:00:00

abstract：:Over a 12-month period, 43 children in eight different wards of our hospital (Hôpital Robert Debré) were infected or colonized with Klebsiella pneumoniae strains producing extended broad-spectrum beta-lactamases. The epidemiology of the outbreak was studied by a molecular approach including the determination of the be...

journal_title：Journal of clinical microbiology

authors： Bingen EH,Desjardins P,Arlet G,Bourgeois F,Mariani-Kurkdjian P,Lambert-Zechovsky NY,Denamur E,Philippon A,Elion J

更新日期：1993-02-01 00:00:00

abstract：:By the use of two reference procedures, an indirect hemagglutination assay and a complement fixation test, the presence or absence of cytomegalovirus (CMV) antibody was determined for 221 human sera. Ninety-nine sera (44.8%) were found to contain CMV antibody. The remaining 122 sera (55.2%) lacked detectable CMV antib...

journal_title：Journal of clinical microbiology

authors： Cheeseman SH,Doern GV,Ferriani RA,Keville MW,McGraw BR,Stewart JA

更新日期：1984-07-01 00:00:00

abstract：:A set of universal degenerate primers which amplified, by PCR, a 600-bp oligomer encoding a portion of the 60-kDa heat shock protein (HSP60) of both Staphylococcus aureus and Staphylococcus epidermidis were developed. However, when used as a DNA probe, the 600-bp PCR product generated from S. epidermidis failed to cro...

journal_title：Journal of clinical microbiology

authors： Goh SH,Potter S,Wood JO,Hemmingsen SM,Reynolds RP,Chow AW

更新日期：1996-04-01 00:00:00

abstract：:Antibiogram typing of methicillin-resistant Staphylococcus aureus with selected antibiotics was evaluated as a primary epidemiological typing tool and compared with ribotyping. Antibiograms were derived with the Kirby-Bauer disk diffusion method by using erythromycin, clindamycin, cotrimoxazole, gentamicin, and ciprof...

journal_title：Journal of clinical microbiology

authors： Blanc DS,Lugeon C,Wenger A,Siegrist HH,Francioli P

更新日期：1994-10-01 00:00:00

abstract：:Monoclonal antibodies to Staphylococcus aureus capsular polysaccharide types 5 and 8 were used in an enzyme-linked immunosorbent assay to serotype 74 and 42 coagulase-negative isolates from cow and goat milk, respectively. Eighteen (15.5%) isolates were typable: 13 Staphylococcus haemolyticus, 1 S. hyicus, 1 S. simula...

journal_title：Journal of clinical microbiology

authors： Poutrel B,Mendolia C,Sutra L,Fournier JM

更新日期：1990-02-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of immunoglobulin G (IgG) to the capsular polysaccharide (CP) of Pasteurella haemolytica serotype 1. Purified CP was first covalently coupled to poly-L-lysine and then optimally adsorbed at a concentration of 5 micrograms/ml to microtiter pla...

journal_title：Journal of clinical microbiology

authors： Townsend EL,Maheswaran SK,Leininger JR,Ames TR

更新日期：1987-02-01 00:00:00

abstract：:Sheep and guinea pigs were immunized with cellular and extracellular antigen from Legionella pneumophila bacteria. After immunization, the animals developed immunoglobulin G titers against the immunizing agent. The same sera were also tested in a Mycoplasma pneumoniae complement fixation test. All the preimmunization ...

journal_title：Journal of clinical microbiology

authors： Berdal BP,Eng J

更新日期：1982-11-01 00:00:00

abstract：:Human bocavirus 1 (HBoV1) is associated with respiratory infections worldwide, mainly in children. Similar to other parvoviruses, it is believed that HBoV1 can persist for long periods of time in humans, probably through maintaining concatemers of the virus single-stranded DNA genome in the nuclei of infected cells. R...

journal_title：Journal of clinical microbiology

authors： Proenca-Modena JL,Paula FE,Buzatto GP,Carenzi LR,Saturno TH,Prates MC,Silva ML,Delcaro LS,Valera FC,Tamashiro E,Anselmo-Lima WT,Arruda E

更新日期：2014-08-01 00:00:00

abstract：:The serological properties of six new pneumococcal capsular types are described. A table listing all 90 pneumococcal types and their cross-reactions is included. ...

journal_title：Journal of clinical microbiology

authors： Henrichsen J

更新日期：1995-10-01 00:00:00

abstract：:We report a study of 1,953 patients whose laboratory records from 1972 through 1983 at the Massachusetts Mycobacteria Reference Laboratory indicated the isolation of Mycobacterium avium complex (MAC) organisms. At least one clinical specimen from each patient during this period exhibited the organism. The incidence of...

journal_title：Journal of clinical microbiology

authors： du Moulin GC,Sherman IH,Hoaglin DC,Stottmeier KD

更新日期：1985-07-01 00:00:00

abstract：:In this report we present clinical descriptions of 12 Hawaiian patients from whom Escherichia vulneris or E. hermannii strains were isolated. All but two patients had soft-tissue infections with multiple bacteria, particularly Staphylococcus aureus. The other two had purulent conjunctivitis associated with S. aureus a...

journal_title：Journal of clinical microbiology

authors： Pien FD,Shrum S,Swenson JM,Hill BC,Thornsberry C,Farmer JJ 3rd

更新日期：1985-08-01 00:00:00

abstract：:In the era after the introduction of the meningococcal serogroup C conjugate vaccine, from 1 January 2003 to 31 December 2010, serogroup B meningococci were the major cause of invasive meningococcal disease in the province of Québec, Canada, being responsible for 72% of all meningococcal disease cases. Of the 334 inva...

journal_title：Journal of clinical microbiology

authors： Zhou J,Lefebvre B,Deng S,Gilca R,Deceuninck G,Law DK,De Wals P,Tsang RS

更新日期：2012-05-01 00:00:00

abstract：:Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussi...

journal_title：Journal of clinical microbiology

authors： Vielemeyer O,Crouch JY,Edberg SC,Howe JG

更新日期：2004-02-01 00:00:00

abstract：:A method of phage subtyping group A type 49 streptococci is described. The method is similar to that used for phage typing staphylococci, except that lysates obtained by induction with mitomycin C rather than propagated stock phages were used. Five type 49 strains were used as phage donors. Seventy-two strains of type...

journal_title：Journal of clinical microbiology

authors： Skjold SA,Wannamaker LW

更新日期：1976-09-01 00:00:00

abstract：:The aim of this study was to determine the distribution in France of the Enterobacter aerogenes prevalent clone isolated in the hospitals of the Marseille area (A. Davin-Regli, D. Monnet, P. Saux, C. Bosi, R. Charrel, A. Barthelemy, and C. Bollet, J. Clin. Microbiol. 34:1474-1480, 1996). A total of 123 E. aerogenes is...

journal_title：Journal of clinical microbiology

authors： Bosi C,Davin-Regli A,Bornet C,Mallea M,Pages JM,Bollet C

更新日期：1999-07-01 00:00:00

abstract：:We previously demonstrated that whole blood contains significantly more hepatitis C virus (HCV) RNA than plasma. To validate the whole-blood-based HCV RNA detection method, a prospective comparison of HCV RNA detection in whole blood and plasma from 50 patients with chronic liver disease was undertaken. Whole-blood an...

journal_title：Journal of clinical microbiology

authors： Stapleton JT,Klinzman D,Schmidt WN,Pfaller MA,Wu P,LaBrecque DR,Han Jq,Phillips MJ,Woolson R,Alden B

更新日期：1999-03-01 00:00:00

abstract：:Better detection of minority human immunodeficiency virus type 1 (HIV-1) populations containing gene mutations may improve the usefulness of antiretroviral resistance testing for clinical management. Molecular cloning of HIV-1 PCR products which might improve minority detection can be slow and difficult, and commercia...

journal_title：Journal of clinical microbiology

authors： Martinez-Picado J,Sutton L,De Pasquale MP,Savara AV,D'Aquila RT

更新日期：1999-09-01 00:00:00

abstract：:CHROMagar Yersinia (CAY) is a new chromogenic medium for the presumptive detection of virulent Yersinia enterocolitica in stools. Based on a comparative analysis of 1,494 consecutive stools from hospitalized patients, CAY was found to be just as sensitive as the reference medium (cefsulodin-irgasan-novobiocin agar) bu...

journal_title：Journal of clinical microbiology

authors： Renaud N,Lecci L,Courcol RJ,Simonet M,Gaillot O

更新日期：2013-04-01 00:00:00

abstract：:The group B streptococcus (GBS) capsular polysaccharide (CPS) is an important virulence factor which is also used for GBS typing. There are 10 CPS types (Ia, Ib, and II to IX). GBS that do not phenotypically type are considered nontypeable. All genes required for CPS synthesis are found on the GBS cps operon, which co...

journal_title：Journal of clinical microbiology

authors： Alhhazmi A,Pandey A,Tyrrell GJ

更新日期：2017-09-01 00:00:00

abstract：:Quantitative fecal lactoferrin was measured in 112 patients tested for toxigenic Clostridium difficile using glutamate dehydrogenase (GDH) and toxin immunoassays combined with tcdB PCR. Lactoferrin levels were higher in the GDH-positive/toxin-positive group (79 μg/ml) than in the GDH-positive/toxin-negative/PCR-positi...

journal_title：Journal of clinical microbiology

authors： LaSala PR,Ekhmimi T,Hill AK,Farooqi I,Perrotta PL

更新日期：2013-01-01 00:00:00

abstract：:Specific antibodies against human immunodeficiency virus (HIV), usually used for diagnosis, almost invariably become detectable within 3 months of exposure. We report on a patient whose HIV infection was identified early by a combined antigen/antibody test, but seroconversion did not occur for 7 months, until the impl...

journal_title：Journal of clinical microbiology

authors： Chin BS,Lee SH,Kim GJ,Kee MK,Suh SD,Kim SS

更新日期：2007-05-01 00:00:00

abstract：:From January 1995 to May 1998, 57 episodes of bacteremia due to viridans group streptococci were identified in 50 febrile neutropenic patients with hematologic malignancies. Four patients experienced two separate episodes of streptococcal bacteremia, and one patient had four separate episodes of streptococcal bacterem...

journal_title：Journal of clinical microbiology

authors： Wisplinghoff H,Reinert RR,Cornely O,Seifert H

更新日期：1999-06-01 00:00:00

abstract：:Three commercial chromogenic agar media were evaluated for Streptococcus agalactiae screening in 200 vaginal swabs from pregnant women. The sensitivity and specificity were 94.3% and 100% for Granada medium (bioMérieux), 100% and 90.3% for Brilliance GBS medium (Thermo Fisher Scientific), and 100% and 98.8% for ChromI...

journal_title：Journal of clinical microbiology

authors： Verhoeven PO,Noyel P,Bonneau J,Carricajo A,Fonsale N,Ros A,Pozzetto B,Grattard F

更新日期：2014-03-01 00:00:00

abstract：:Hepatitis B virus (HBV) has eight genotypes which have distinct geographical distributions. Studies comparing differences in the clinical outcomes of infections caused by strains with genotype-related variations in the HBV genome have largely compared genotypes B and C and genotypes A and D but not all four genotypes....

journal_title：Journal of clinical microbiology

authors： Kidd-Ljunggren K,Myhre E,Bläckberg J

更新日期：2004-12-01 00:00:00

abstract：:Sequencing-based pathogen identification directly from clinical specimens requires time-consuming interpretation, especially with mixed chromatograms when multiple microorganisms are detected. We assessed RipSeq Mixed software for human papillomavirus (HPV) genotyping by comparison to the linear array HPV genotyping a...

journal_title：Journal of clinical microbiology

authors： Tardif KD,Simmon KE,Kommedal O,Pyne MT,Schlaberg R

更新日期：2013-04-01 00:00:00

abstract：:Real-time quantitative PCR (qPCR) assay of sputum or nasopharyngeal specimens has shown promising results in the detection of pneumococcal community-acquired pneumonia (PncCAP). We applied qPCR for the autolysin gene (lytA) and compared sputum and nasopharyngeal swab (NPS) pneumococcal loads in elderly patients with c...

journal_title：Journal of clinical microbiology

authors： Saukkoriipi A,Palmu AA,Pascal T,Verlant V,Hausdorff WP,Jokinen J

更新日期：2017-12-26 00:00:00

abstract：:BHI agars supplemented with vancomycin 4 (BHI-V4) and 3 (BHI-V3) mg/liter have been proposed for screening vancomycin intermediately susceptible Staphylococcus aureus (VISA) and heteroresistant (hVISA) phenotypes, respectively, but growth interpretation criteria have not been established. We reviewed the growth result...

journal_title：Journal of clinical microbiology

authors： Khatib R,Riederer K,Sharma M,Shemes S,Iyer SP,Szpunar S

更新日期：2015-11-01 00:00:00

abstract：:Rapid tests for detection of methicillin-resistant Staphylococcus aureus (MRSA) carriage are important to limit the transmission of MRSA in the health care setting. We evaluated the performance of the BD GeneOhm MRSA real-time PCR assay using a diverse collection of MRSA isolates, mainly from Copenhagen, Denmark, but ...

journal_title：Journal of clinical microbiology

authors： Bartels MD,Boye K,Rohde SM,Larsen AR,Torfs H,Bouchy P,Skov R,Westh H

更新日期：2009-05-01 00:00:00

abstract：:A PCR method for the unequivocal assignment of Haemophilus influenzae capsular type (types a to f) was developed. PCR primers were designed from capsule type-specific DNA sequences cloned from the capsular gene cluster of each of the six capsular types. PCR product was amplified only from the capsular type for which t...

journal_title：Journal of clinical microbiology

authors： Falla TJ,Crook DW,Brophy LN,Maskell D,Kroll JS,Moxon ER

更新日期：1994-10-01 00:00:00