Abstract：
:CDC eugonic oxidizer group 2 (EO-2) is a group of unclassified gram-negative bacterial strains isolated from various human sources. As determined by biochemical tests and analyses of fatty acid compositions, these organisms form a homogeneous group that appears to be distinct from but related to other Paracoccus species. Molecular studies were performed on a set of 13 EO-2 strains from various clinical sources and geographic locations in the United States and Canada to determine their relationship to the Paracoccus genus. Control strains were Paracoccus denitrificans ATCC 17741(T), P. versutus ATCC 25364(T), P. aminophilus ATCC 49673(T), P. solventivorans ATCC 700252(T), and Psychrobacter immobilis ATCC 43116(T), which are phenotypically similar to EO-2. Nearly complete (1,500-base) 16S rRNA gene sequencing of eight EO-2 strains showed a high level of sequence similarity (>99.3%) within the group, and a BLAST search of GenBank placed the EO-2 cluster in close proximity to Paracoccus species (95 to 97% similarity). DNA-DNA hybridization studies of 13 of the EO-2 strains showed all to be related at the species level, with >70% relatedness under stringent conditions and a divergence within the group of less than 2%. None of the Paracoccus control strains hybridized at >54% with any of the EO-2 strains. These results indicate that EO-2 represents a new Paracoccus species, the first isolated from human clinical specimens. A new species, Paracoccus yeeii, is proposed for the EO-2 strains. The type strain of P. yeeii is CDCG1212 (ATCC BAA-599 and CCUG 46822), isolated in Pennsylvania from dialysate of a 77-year-old male with peritonitis.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Daneshvar MI,Hollis DG,Weyant RS,Steigerwalt AG,Whitney AM,Douglas MP,Macgregor JP,Jordan JG,Mayer LW,Rassouli SM,Barchet W,Munro C,Shuttleworth L,Bernard Kdoi
10.1128/jcm.41.3.1289-1294.2003subject
Has Abstractpub_date
2003-03-01 00:00:00pages
1289-94issue
3eissn
0095-1137issn
1098-660Xjournal_volume
41pub_type
杂志文章abstract：:A proficiency review of antituberculous drug susceptibility testing (DST) was undertaken by the regional tuberculosis reference laboratories of the Western Pacific Region of WHO to evaluate the performance of national reference laboratories (NRLs) and to ensure that the results from the participating laboratories are ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.00784-07
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abstract：:Identification of clinical isolates of Nocardia to the species level is important for defining the spectrum of disease produced by each species and for predicting antimicrobial susceptibility. We evaluated the usefulness of PCR amplification of a portion of the Nocardia 16S rRNA gene and subsequent restriction endonuc...
journal_title：Journal of clinical microbiology
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doi：
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abstract：:The development of a novel normalized quantitative competitive real-time PCR on the LightCycler instrument (NQC-LC-PCR) and its application to the quantification of cytomegalovirus (CMV) DNA in clinical samples are described. A heterologous competitor DNA was spiked into test samples and served as an internal amplific...
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doi：10.1128/jcm.40.12.4547-4553.2002
更新日期：2002-12-01 00:00:00
abstract：:Real-time PCR is dependent upon a calibration function for quantification. While long-term storage of standards saves cost and time, solutions of DNA are prone to degradation. We present here the benchmark treatment for preservation of DNA standards, involving storage in 50% glycerol-double-distilled water, whereby a ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.01230-10
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.28.5.837-840.1990
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abstract：:Cryptococcus neoformans var. neoformans (serotype D) and C. neoformans var. grubii (serotype A) differ in geographic prevalence and dermatotropism, with C. neoformans var. neoformans strains being more prevalent among isolates from temperate countries as well as from skin infections. Analysis of 19 strains from each s...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.39.9.3365-3367.2001
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abstract：:Tularemia is the zoonotic disease caused by the gram-negative coccobacillus Francisella tularensis. Its wide distribution in the environment poses a challenge for understanding the transmission, ecology, and epidemiology of the disease. F. tularensis is also considered a potential biological weapon due to its extreme ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.41.12.5492-5499.2003
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journal_title：Journal of clinical microbiology
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doi：10.1128/JCM.00157-08
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.25.5.958-959.1987
更新日期：1987-05-01 00:00:00
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.41.9.4231-4237.2003
更新日期：2003-09-01 00:00:00
abstract：:In this study, 102 herpes simplex virus isolates were typed by cell culture selection (chicken embryo cells and guinea pig embryo cells [CE/GPE]), (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) sensitivity, plaque reduction neutralization, and indirect immunofluorescence staining techniques. The percentages of agreement ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.17.3.396-399.1983
更新日期：1983-03-01 00:00:00
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.00222-07
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.13.4.618-626.1981
更新日期：1981-04-01 00:00:00
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.37.3.675-680.1999
更新日期：1999-03-01 00:00:00
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.02086-09
更新日期：2010-03-01 00:00:00
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.27.8.1707-1710.1989
更新日期：1989-08-01 00:00:00
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.11.4.424-425.1980
更新日期：1980-04-01 00:00:00
abstract：:Simple enzymatic assays to detect heat-labile enterotoxins whose modes of action are similar to that of cholera toxin were evaluated. The assays are performed by using an artificial substrate, diethylamino benzylidine-aminoguanidine, which is an ADP-ribose acceptor. The product, formed in the presence of NAD+, can be ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.27.11.2414-2419.1989
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.41.9.4480-4482.2003
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journal_title：Journal of clinical microbiology
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.30.4.1024-1026.1992
更新日期：1992-04-01 00:00:00
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.34.3.641-646.1996
更新日期：1996-03-01 00:00:00
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.30.2.407-410.1992
更新日期：1992-02-01 00:00:00
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.28.12.2612-2615.1990
更新日期：1990-12-01 00:00:00
abstract：:The ability of Yersinia to digest polypectate may be of some value in differentiating Y. enterocolitica and Y. pseudotuberculosis from some of the other fermenting gram-negative bacilli, such as Enterobacter agglomerans, with which they can be confused. Pectolytic activity in Yersinia may also have some teleologic or ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1975-12-01 00:00:00
abstract：:A case of pyogenic liver abscess (PLA) due to Rhodococcus equi in an immunocompetent individual was successfully treated by combining surgery and antibiotics. The R. equi-targeted antimicrobial agents erythromycin and rifampin were used only after surgical resection of the lesion and identification of the infective or...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.43.2.1002-1004.2005
更新日期：2005-02-01 00:00:00
abstract：:We describe the isolation of Actinobacillus lignieresii and an A. equuli-like bacterium from an infected horse-bite wound in a 22-year-old stable foreman and A. suis from a bite injury in a 35-year-old man who had been attacked by a horse. A. lignieresii was also isolated in pure culture from an infected sheep-bite wo...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.29.11.2535-2538.1991
更新日期：1991-11-01 00:00:00
abstract：:The enzyme-linked immunosorbent assay (ELISA) has been proposed as an alternative serologic diagnostic test to the indirect immunofluorescence assay (IFA) for scrub typhus. Here, we systematically determine the optimal sample dilution and cutoff optical density (OD) and estimate the accuracy of IgM ELISA using Bayesia...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.02744-15
更新日期：2016-06-01 00:00:00
abstract：:The usefulness of filter paper for preservation of bacterial cells was shown by mixed-linker DNA fingerprint analysis of Mycobacterium tuberculosis isolates from 77 Brazilian patients. DNA fingerprints of samples spotted onto filter paper and conventional culture material were identical. Thus, filter paper specimens a...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.36.2.573-576.1998
更新日期：1998-02-01 00:00:00
abstract：:We conducted a molecular epidemiology study on 120 Mycobacterium tuberculosis isolates from patients presenting pulmonary tuberculosis (TB) in Burkina Faso. Classical antibiogram studies and genetic characterization, using mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing and ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.01918-06
更新日期：2007-03-01 00:00:00
