Characterization of a coronavirus isolated from a diarrheic foal.

abstract：:PCR has greatly facilitated pertussis diagnosis due to the speed, sensitivity, and specificity of this assay compared to other detection methods. Various single-target PCR assays are currently utilized, but none is universally considered to be the "gold standard." Our aim was to assess the use of multitarget versus si...

journal_title：Journal of clinical microbiology

authors： Qin X,Galanakis E,Martin ET,Englund JA

更新日期：2007-02-01 00:00:00

abstract：:The microflora associated with three dentoalveolar abscesses was determined by cultural and molecular methods. 16S rRNA genes were randomly amplified by means of conserved eubacterial primers and cloned. Restriction fragment length polymorphism analysis of the clones and amplified genes encoding 16S rRNA from the cult...

journal_title：Journal of clinical microbiology

authors： Dymock D,Weightman AJ,Scully C,Wade WG

更新日期：1996-03-01 00:00:00

abstract：:Restriction endonuclease analysis of virulence plasmid DNA was used to study the epidemiology of Yersinia pseudotuberculosis infections. The origin of Y. pseudotuberculosis could be divided into two focus areas: Eastern Asia and Europe. Wild animals were an important reservoir for the Y. pseudotuberculosis seen in inf...

journal_title：Journal of clinical microbiology

authors： Fukushima H,Gomyoda M,Kaneko S,Tsubokura M,Takeda N,Hongo T,Shubin FN

更新日期：1994-05-01 00:00:00

abstract：:A total of 410 well-defined Campylobacter, Helicobacter, and Wolinella strains, comprising 26 named species, subspecies, and defined groups, were tested for indoxyl acetate hydrolysis by a disk method by using disks prepared at the Centers for Disease Control, Atlanta, Ga. All C. coli (43 strains), C. cryaerophila (34...

journal_title：Journal of clinical microbiology

authors： Popovic-Uroic T,Patton CM,Nicholson MA,Kiehlbauch JA

更新日期：1990-10-01 00:00:00

abstract：:The prevalence of the Epstein-Barr virus (EBV) Taiwan variant was investigated in the throat washing (TW) samples from patients with head and neck tumors, persons with nonmalignant diseases, and healthy adults in Taiwan. By using the EBV (BNLF-1 gene)-specific primers and PCR, the EBV latent membrane protein gene BNLF...

journal_title：Journal of clinical microbiology

authors： Jeng KC,Hsu CY,Liu MT,Chung TT,Liu ST

更新日期：1994-01-01 00:00:00

abstract：:A neutralizing monoclonal antibody, B37:1, against a human rotavirus (B37) with a "super-short" RNA pattern was derived. The antibody gave serotype-specific reactions with a panel of standard rotaviruses and was reactive in an enzyme immunoassay. Epidemiological studies of this new human serotype should now be possibl...

journal_title：Journal of clinical microbiology

authors： Tursi JM,Albert MJ,Bishop RF

更新日期：1987-12-01 00:00:00

abstract：:Sequence-specific PCR was used in six laboratories and a ligase detection reaction was used in one laboratory to detect the zidovudine-resistance mutation at codon 215 of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase DNA. The genotypes of 27 different clinical samples, including cultured HIV-1 isol...

journal_title：Journal of clinical microbiology

authors： Shafer RW,Winters MA,Mayers DL,Japour AJ,Kuritzkes DR,Weislow OS,White F,Erice A,Sannerud KJ,Iversen A,Pena F,Dimitrov D,Frenkel LM,Reichelderfer PS

更新日期：1996-07-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) method for the rapid detection of serotype 1 Marek's disease virus (MDV) was developed. The method used a set of three pairs of primers to amplify the MEQ gene for detecting serotype 1 MDV. The MDV LAMP method did not cross-react with serotype 2 and serotype 3, nor did t...

journal_title：Journal of clinical microbiology

authors： Angamuthu R,Baskaran S,Gopal DR,Devarajan J,Kathaperumal K

更新日期：2012-03-01 00:00:00

abstract：:Typing and in vitro cultivation of rotavirus-positive human stool samples stored unintentionally at ambient tropical temperatures for 2 1/2 months showed that rotavirus is stable and may still be infectious in vitro. This indicates that stool specimen collection for rotavirus studies can be performed in areas and sett...

journal_title：Journal of clinical microbiology

authors： Fischer TK,Steinsland H,Valentiner-Branth P

更新日期：2002-12-01 00:00:00

abstract：:Sixteen metabolically deficient dwarf-colony mutants of Escherichia coli were isolated from urine culture and represented about 2% of all E. coli isolated during the same period. In 14 cases, mutants were isolated from debilitated patients: elderly persons or patients in the terminal stages of a chronic disease. In 15...

journal_title：Journal of clinical microbiology

authors： Borderon E,Horodniceanu T

更新日期：1978-12-01 00:00:00

abstract：:Determination of anti-hepatitis E virus (anti-HEV) antibodies is still enigmatic. There is no gold standard, and results obtained with different assays often diverge. Herein, five assays were compared for detection of anti-HEV IgM and IgG. Serum samples from 500 Swedish blood donors and 316 patients, of whom 136 had s...

journal_title：Journal of clinical microbiology

authors： Norder H,Karlsson M,Mellgren Å,Konar J,Sandberg E,Lasson A,Castedal M,Magnius L,Lagging M

更新日期：2016-03-01 00:00:00

abstract：:Accreditation in Ontario, Canada, requires that licensed clinical laboratories participate in external quality assessment (also known as proficiency testing) and perform competency evaluation of their staff. To assess the extent of ongoing competency assessment practices, the Quality Management Program--Laboratory Ser...

journal_title：Journal of clinical microbiology

authors： Desjardins M,Fleming CA

更新日期：2014-08-01 00:00:00

abstract：:We present a duplex, real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with r...

journal_title：Journal of clinical microbiology

authors： Cunningham SA,Noorie T,Meunier D,Woodford N,Patel R

更新日期：2013-04-01 00:00:00

abstract：:We report a case of pneumonia with a fatal outcome caused by a nonhemolytic strain of Streptococcus pyogenes. This strain was isolated in pure growth from blood cultures and was fully identified biochemically. Such strains will be difficult to recognize and isolate from sites with heavy growth of normal flora. This ph...

journal_title：Journal of clinical microbiology

authors： Taylor MB,Barkham T

更新日期：2002-06-01 00:00:00

abstract：:Whole-house surveillance for healthcare-associated infection is no longer the recommended practice because of the large personnel time investment required. We developed a computer-based tracking system using microbiologic data as an aid in detecting potential outbreaks of healthcare-associated infections on a hospital...

journal_title：Journal of clinical microbiology

authors： Hacek DM,Cordell RL,Noskin GA,Peterson LR

更新日期：2004-03-01 00:00:00

abstract：:A semisolid medium (designated Serratia differentiation medium) containing L-arabinose, ornithine, and selective inhibitor was used to differentiate three clinically encountered Serratia species. The inhibitor, Irgasan DP-300, was incorporated to eliminate false-positive reactions from most remaining Enterobacteriacea...

journal_title：Journal of clinical microbiology

authors： Gibson S,Friedman H

更新日期：1978-03-01 00:00:00

abstract：:Several studies have identified thymidine excess in susceptibility test media as the cause of spurious resistance of various bacteria to sulfamethoxazole-trimethoprim. We document the phenomenon in Salmonella typhi and Salmonella paratyphi-A and demonstrate its occurrence in 3 of 17 (18%) lots of Mueller-Hinton agars ...

journal_title：Journal of clinical microbiology

authors： Escamilla J,Kilpatrick ME,Ugarte HF

更新日期：1986-01-01 00:00:00

abstract：:Since the late 1980s several studies have described the increased incidence and severity of invasive group A streptococcal (GAS) infections. However, most studies on GAS pathogenesis have focused on information obtained during outbreaks. We analyzed isolate distribution and host susceptibility as part of a nationwide ...

journal_title：Journal of clinical microbiology

authors： Ekelund K,Darenberg J,Norrby-Teglund A,Hoffmann S,Bang D,Skinhøj P,Konradsen HB

更新日期：2005-07-01 00:00:00

abstract：:A duplex real-time SYBR Green LightCycler PCR (LC-PCR) assay with DNA extraction using the QIAamp DNA Stool Mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. The protocol used the same LC-PCR with 20 pairs of specific primers. Th...

journal_title：Journal of clinical microbiology

authors： Fukushima H,Tsunomori Y,Seki R

更新日期：2003-11-01 00:00:00

abstract：:The longus type IV pilus structural gene (lngA) was sought among 217 clinical enterotoxigenic Escherichia coli (ETEC) strains isolated in Argentina. lngA was present in 20.7% of the isolates and was highly associated with ETEC producing heat-stable toxin and the most common colonization factors. The prevalence of long...

journal_title：Journal of clinical microbiology

authors： Pichel MG,Binsztein N,Qadri F,Girón JA

更新日期：2002-02-01 00:00:00

abstract：:A total of 1,335 Escherichia coli strains isolated from patients with diarrhea were submitted to the Serény test. Of these strains, 1,035 were also submitted to slide agglutination test, using antisera for invasive E. coli serogroups. Of 325 isolates that were lysine decarboxylase negative, 138 reacted with one of the...

journal_title：Journal of clinical microbiology

authors： Toledo MR,Trabulsi LR

更新日期：1983-03-01 00:00:00

abstract：:A Pasteurella multocida species-specific oligonucleotide probe, pmhyb449, targeting 16S rRNA was designed and evaluated by whole-cell hybridization against 22 selected reference strains in animal tissues. It differentiated P. multocida from other bacterial species of the families Pasteurellaceae and Enterobacteriaceae...

journal_title：Journal of clinical microbiology

authors： Mbuthia PG,Christensen H,Boye M,Petersen KM,Bisgaard M,Nyaga PN,Olsen JE

更新日期：2001-07-01 00:00:00

abstract：:Three molecular typing methods were used to study the relationships among 184 Campylobacter strains isolated from humans, cattle, and chickens. All strains were genotyped by amplified fragment length polymorphism (AFLP) analysis, multilocus sequence typing (MLST), and sequence analysis of a genomic region with short t...

journal_title：Journal of clinical microbiology

authors： Schouls LM,Reulen S,Duim B,Wagenaar JA,Willems RJ,Dingle KE,Colles FM,Van Embden JD

更新日期：2003-01-01 00:00:00

abstract：:A simple new agar medium containing L-canavanine, glycine, and bromthymol blue was found to give a clearer and more accurate distinction between serotype A or D (Cryptococcus neoformans var. neoformans) and serotype B or C (C. neoformans var. gattii) than creatinine-dextrose-bromthymol blue or glycine-cycloheximide-ph...

journal_title：Journal of clinical microbiology

authors： Kwon-Chung KJ,Polacheck I,Bennett JE

更新日期：1982-03-01 00:00:00

abstract：:Direct enzyme-linked immunosorbent assay methods offer several advantages in assessing past or recent exposure to cytomegalovirus (CMV) infection, but there persist many pitfalls in the use of these methods for determining specific immunoglobulin M (IgM). The efficiency of absorption of sera by IgG-coated latex beads,...

journal_title：Journal of clinical microbiology

authors： Joassin L,Reginster M

更新日期：1986-03-01 00:00:00

abstract：:The classification of viruses is relevant to a number of scientific and clinical disciplines, including the practice of diagnostic virology. Here, we provide an update to our previous review of taxonomic changes for disease-causing viruses in humans and vertebrate animals, covering changes between 2018 and 2020. Recen...

journal_title：Journal of clinical microbiology

authors： Loeffelholz MJ,Fenwick BW

更新日期：2021-01-21 00:00:00

abstract：:Between November 1983 and January 1984, 13 cases of Candida parapsilosis endophthalmitis occurred in Florida, Georgia, and Tennessee in patients who had had an intraocular lens implantation (IOLI) or cataract extraction with an IOLI. This outbreak followed the introduction in July 1983 of a new brand of balanced salt ...

journal_title：Journal of clinical microbiology

authors： McCray E,Rampell N,Solomon SL,Bond WW,Martone WJ,O'Day D

更新日期：1986-10-01 00:00:00

abstract：:In a large prospective comparison, the illumigene test detected Clostridium difficile in 98% of toxin-positive and 58% of toxin-negative samples confirmed positive by other methods. The Xpert was uniformly sensitive. Most samples with discrepant results had C. difficile concentrations below the illumigene limit of det...

journal_title：Journal of clinical microbiology

authors： Gyorke CE,Wang S,Leslie JL,Cohen SH,Solnick JV,Polage CR

更新日期：2013-01-01 00:00:00

abstract：:We compared a nested PCR assay and microscopic examination of Giemsa-stained blood films for detection and identification of Plasmodium spp. in blood specimens. PCR was more sensitive than microscopy and capable of identifying malaria parasites at the species level when microscopy was equivocal. ...

journal_title：Journal of clinical microbiology

authors： Johnston SP,Pieniazek NJ,Xayavong MV,Slemenda SB,Wilkins PP,da Silva AJ

更新日期：2006-03-01 00:00:00

abstract：:In an attempt to characterize an unusual mycobacterial isolate from a 44-year-old patient living in France, we applied phenotypic characterizations and various previously described molecular methods for the taxonomic classification of mycobacteria. The results of the investigations were compared to those obtained in a...

journal_title：Journal of clinical microbiology

authors： Chemlal K,Huys G,Laval F,Vincent V,Savage C,Gutierrez C,Laneelle MA,Swings J,Meyers WM,Daffe M,Portaels F

更新日期：2002-07-01 00:00:00