Serologic analysis of human rotavirus serotypes P1A and P2 by using monoclonal antibodies.

abstract：:A swine H3N2 (swH3N2) and pandemic (H1N1) 2009 (pH1N1) influenza A virus reassortant (swH3N2/pH1N1) was detected in Canadian swine at the end of 2010. Simultaneously, a similar virus was also detected in Canadian mink based on partial viral genome sequencing. The origin of the new swH3N2/pH1N1 viral genes was related ...

journal_title：Journal of clinical microbiology

authors： Tremblay D,Allard V,Doyon JF,Bellehumeur C,Spearman JG,Harel J,Gagnon CA

更新日期：2011-12-01 00:00:00

abstract：:The cellular fatty acid composition of Legionella feeleii was determined by capillary gas-liquid chromatography, and the ubiquinone content was determined by reverse-phase high-pressure liquid chromatography. As in other Legionella species, this new species is characterized by relatively large amounts of branched-chai...

journal_title：Journal of clinical microbiology

authors： Moss CW,Bibb WF,Karr DE,Guerrant GO,Lambert MA

更新日期：1983-10-01 00:00:00

abstract：:Sodium polyanethole sulfonate (SPS; trade name, Liquoid) is a constituent in culture media used to grow bacteria from blood samples from patients suspected of bacteremia. SPS prevents the killing of bacteria by innate cellular and humoral factors. We analyzed the effect of SPS on the three complement activation pathwa...

journal_title：Journal of clinical microbiology

authors： Palarasah Y,Skjoedt MO,Vitved L,Andersen TE,Skjoedt K,Koch C

更新日期：2010-03-01 00:00:00

abstract：:Bovine tuberculosis (bTB) caused by Mycobacterium bovis is a transmissible disease notifiable to the World Organization for Animal Health and to the European Union, with ongoing efforts of surveillance and eradication in every EU member state. In Germany, a country which has been declared officially free from bovine t...

journal_title：Journal of clinical microbiology

authors： Kohl TA,Utpatel C,Niemann S,Moser I

更新日期：2018-08-27 00:00:00

abstract：:Of 216 fecal and intestinal samples collected from nursing or weaned diarrheic pigs in the United States and Canada, 57 were identified as group A rotavirus positive by RNA electrophoresis and silver staining. Fifty-seven and 52 rotavirus-positive samples were analyzed by hybridization with Gottfried and OSU PCR-deriv...

journal_title：Journal of clinical microbiology

authors： Rosen BI,Parwani AV,Lopez S,Flores J,Saif LJ

更新日期：1994-02-01 00:00:00

abstract：:Staphylococcal cassette chromosome mec (SCCmec) types and Panton-Valentine leukocidin (PVL) gene carriage were compared among suspected community-associated methicillin-resistant Staphylococcus aureus MRSA (CA-MRSA) and health care-associated MRSA (HA-MRSA) isolates. CA-MRSA isolates carried the SCCmec type IV complex...

journal_title：Journal of clinical microbiology

authors： Moroney SM,Heller LC,Arbuckle J,Talavera M,Widen RH

更新日期：2007-03-01 00:00:00

abstract：:A set of 104 isolates from human clinical samples from the United States, morphologically compatible with Bipolaris, were morphologically and molecularly identified through the sequence analysis of the internal transcribed space (ITS) region of the nuclear ribosomal DNA (rDNA). The predominant species was Bipolaris sp...

journal_title：Journal of clinical microbiology

authors： da Cunha KC,Sutton DA,Fothergill AW,Cano J,Gené J,Madrid H,De Hoog S,Crous PW,Guarro J

更新日期：2012-12-01 00:00:00

abstract：:Fifty staphylococcal strains that produced toxic shock syndrome (TSS) toxin 1 and that were isolated from patients with TSS were characterized. One strain had more properties that were characteristic of Staphylococcus hyicus than of Staphylococcus aureus. Forty-four strains had the same properties or differed in only ...

journal_title：Journal of clinical microbiology

authors： Aliu B,Bergdoll MS

更新日期：1988-11-01 00:00:00

abstract：:The ability of Yersinia to digest polypectate may be of some value in differentiating Y. enterocolitica and Y. pseudotuberculosis from some of the other fermenting gram-negative bacilli, such as Enterobacter agglomerans, with which they can be confused. Pectolytic activity in Yersinia may also have some teleologic or ...

journal_title：Journal of clinical microbiology

authors： von Riesen VL

更新日期：1975-12-01 00:00:00

abstract：:The detection of amplified products resulting from polymerase chain reactions (PCRs) remains a complicated process. To simplify the detection procedures, we developed a colorimetric microtiter plate hybridization assay for the specific detection of 5'-biotinylated PCR fragments of Mycobacterium leprae DNA. For this as...

journal_title：Journal of clinical microbiology

authors： van der Vliet GM,Hermans CJ,Klatser PR

更新日期：1993-03-01 00:00:00

abstract：:During an investigation of a hospital outbreak of norovirus gastroenteritis identified as being caused by a recombinant genogroup II (rGII-3a) strain, fecal specimens collected from asymptomatic staff and patients were tested by nested PCR. A GII-4 norovirus strain, the predominant strain associated with outbreaks in ...

journal_title：Journal of clinical microbiology

authors： Gallimore CI,Cubitt D,du Plessis N,Gray JJ

更新日期：2004-05-01 00:00:00

abstract：:We compared examination of unstained and Gram-stained smears as methods of evaluating sputum quality. Of 100 slides examined, 96 (96%) were graded identically. Each discrepancy between methods was near the cutoff for specimen acceptability. We concluded that examination of unstained smears can provide an accurate, cos...

journal_title：Journal of clinical microbiology

authors： Wetterau LM,Zeimis RT,Hollick GE

更新日期：1986-07-01 00:00:00

abstract：:Between January 1988 and December 1989 Aeromonas species were isolated from 45 (1.8%) of 2,480 patients with acute gastroenteritis. No other bacterial enteric pathogens were found in any of these 45 patients. Of the 45 Aeromonas isolates, 35 strains (77.8%) were Aeromonas hydrophila, 7 (15.5%) were Aeromonas sobria, a...

journal_title：Journal of clinical microbiology

authors： Deodhar LP,Saraswathi K,Varudkar A

更新日期：1991-05-01 00:00:00

abstract：:The PathoTec Rapid I-D System for identifying Enterobacteriaceae was evaluated with 471 cultures. In 4,910 individual test comparisons, 95.5% of the results agreed, with results of only two test strips, those for esculin hydrolysis and urease production, agreeing with conventional tests in less than 94% of the trials....

journal_title：Journal of clinical microbiology

authors： Smith PB,Rhoden DL,Tomfohrde KM

更新日期：1975-04-01 00:00:00

abstract：:The continuous spread of community-acquired methicillin-resistant Staphylococcus aureus (caMRSA) and the introduction of these highly virulent isolates into hospitals represent increasing threats. The timely recognition of caMRSA strains is crucial for infection control purposes. Thus, we developed a PCR-based assay f...

journal_title：Journal of clinical microbiology

authors： Strommenger B,Braulke C,Pasemann B,Schmidt C,Witte W

更新日期：2008-02-01 00:00:00

abstract：:Anyplex STI-7 is a new molecular kit that detects seven sexually transmitted pathogens. Among 202 subjects screened for genital infection, 143 (70.4%) were diagnosed with at least one pathogen, in concordance with reference methods. In addition, the Anyplex STI-7 demonstrated coinfections, such as that with Ureaplasma...

journal_title：Journal of clinical microbiology

authors： Berçot B,Amarsy R,Goubard A,Aparicio C,Loeung HU,Segouin C,Gueret D,Jacquier H,Meunier F,Mougari F,Cambau E

更新日期：2015-03-01 00:00:00

abstract：:The rate of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis (TB) has been steadily increasing in countries of the former USSR. The availability of rapid and reliable methods for the detection of drug resistance to second-line drugs is vital for adequate patient management. We evaluated the ...

journal_title：Journal of clinical microbiology

authors： Ignatyeva O,Kontsevaya I,Kovalyov A,Balabanova Y,Nikolayevskyy V,Toit K,Dragan A,Maxim D,Mironova S,Kummik T,Muntean I,Koshkarova E,Drobniewski F

更新日期：2012-05-01 00:00:00

abstract：:A new immunochromatographic rapid test, Determine HIV-1/2, for the detection of antibodies to human immunodeficiency virus type 1 (HIV-1) and HIV-2 in human whole blood, serum, and plasma was evaluated. Determine HIV-1/2 is a sandwich immunoassay and uses a nitrocellulose strip with a capture site for the patient's re...

journal_title：Journal of clinical microbiology

authors： Arai H,Petchclai B,Khupulsup K,Kurimura T,Takeda K

更新日期：1999-02-01 00:00:00

abstract：:The replication of human coronavirus strain 229E was observed by using indirect immunofluorescence in infected monolayers of MRC continuous cells. By 8 h after infection, bright cytoplasmic fluorescence was detected in cells infected with human coronavirus 229E. Discrete foci of infection were observed from 8 to 16 h ...

journal_title：Journal of clinical microbiology

authors： Macnaughton MR,Thomas BJ,Davies HA,Patterson S

更新日期：1980-09-01 00:00:00

abstract：:Helicobacter pylori is known to be a major cause of peptic ulceration. The jhp0562 gene, encoding a glycosyltransferase involved in the synthesis of the lipopolysaccharide, was associated with peptic ulcer disease (PUD) in children. The beta-(1,3)-galactosyltransferase [beta-(1,3)GalT] gene (jhp0563), involved in Lewi...

journal_title：Journal of clinical microbiology

authors： Oleastro M,Santos A,Cordeiro R,Nunes B,Mégraud F,Ménard A

更新日期：2010-08-01 00:00:00

abstract：:Commercially prepared fluorescein-labeled antihuman antibodies were labed with 125I and used to compre specific herpes simplex virus antibody titers as determined by indirect fluorescent antibody and radioimmunoassay techniques. Total virus-specific immunoglobulin and virus-specific immunoglobulin G titers did not var...

journal_title：Journal of clinical microbiology

authors： Parkinson AJ,Kalmakoff J

更新日期：1976-06-01 00:00:00

abstract：:Gram stains remain the cornerstone of diagnostic testing in the microbiology laboratory for the guidance of empirical treatment prior to availability of culture results. Incorrectly interpreted Gram stains may adversely impact patient care, and yet there are no comprehensive studies that have evaluated the reliability...

journal_title：Journal of clinical microbiology

authors： Samuel LP,Balada-Llasat JM,Harrington A,Cavagnolo R

更新日期：2016-06-01 00:00:00

abstract：:A total of 49 adenovirus type 3 (Ad3) strains from patients with epidemic keratoconjunctivitis at an eye clinic in Sapporo, Japan, from 1983 to 1986 were classified into either genome type Ad3f (26 strains) or Ad3g (23 strains). By the use of Hinf1 and TaqI restriction endonucleases, they were classified into 11 and 3...

journal_title：Journal of clinical microbiology

authors： Itakura S,Aoki K,Sawada H,Shinagawa M

更新日期：1990-10-01 00:00:00

abstract：:During pregnancy, Plasmodium falciparum infection of the placenta frequently occurs in the absence of parasites in peripheral blood. We investigated the abilities of the OptiMAL rapid immunochromatographic strip test for P. falciparum lactate dehydrogenase and species-specific PCR performed on peripheral blood to dete...

journal_title：Journal of clinical microbiology

authors： Mankhambo L,Kanjala M,Rudman S,Lema VM,Rogerson SJ

更新日期：2002-01-01 00:00:00

abstract：:The impact of the viability of Trichomonas vaginalis in urine on wet mount, culture, and PCR methods was assessed. To minimize the chance of false-negative results, urine specimens should be processed within 30 min of specimen collection and maintained at 37 degrees C, since temperature appears to affect the viability...

journal_title：Journal of clinical microbiology

authors： Shafir SC,Sorvillo FJ

更新日期：2006-10-01 00:00:00

abstract：:A new latex agglutination test (Pastorex Staph-Plus, Sanofi Diagnostics Pasteur), consisting of a mixture of latex particles coated with fibrinogen and immunoglobulin G for the detection of clumping factor and protein A and latex particles sensitized with monoclonal antibodies directed to Staphylococcus aureus serotyp...

journal_title：Journal of clinical microbiology

authors： Fournier JM,Bouvet A,Mathieu D,Nato F,Boutonnier A,Gerbal R,Brunengo P,Saulnier C,Sagot N,Slizewicz B

更新日期：1993-05-01 00:00:00

abstract：:Participants in the College of American Pathologists' Mycobacteriology E proficiency testing survey in 1993 were asked to complete a questionnaire addressing mycobacterial test methods, test volume, and frequency of detection of drug-resistant Mycobacterium tuberculosis (MTB). A similar questionnaire had been distribu...

journal_title：Journal of clinical microbiology

authors： Woods GL,Witebsky FG

更新日期：1995-02-01 00:00:00

abstract：:Testing for susceptibility to pyrazinamide (PZA) and analysis of the pncA gene sequences of 423 Mycobacterium tuberculosis complex isolates have revealed a unique silent nucleotide substitution that enables the rapid identification of "M. canettii" (proposed name). Moreover, the lack of a defined mutation within the p...

journal_title：Journal of clinical microbiology

authors： Somoskovi A,Dormandy J,Parsons LM,Kaswa M,Goh KS,Rastogi N,Salfinger M

更新日期：2007-02-01 00:00:00

abstract：:To facilitate the identification and serotyping of Salmonella species, we established a wide variety of murine monoclonal antibodies (MAbs) that were reactive with the lipopolysaccharides (LPSs) of Salmonella serogroups B to E. An effective approach for generating LPS-reactive hybridomas was used; this required immuni...

journal_title：Journal of clinical microbiology

authors： Luk JM,Lindberg AA

更新日期：1991-11-01 00:00:00

abstract：:We evaluated the utility of the commercial Allplex genital ulcer real-time PCR multiplex assay for detecting Treponema pallidum, herpes simplex virus 1 (HSV-1) and 2 (HSV-2), and Chlamydia trachomatis serovar L (lymphogranuloma venereum [LGV]) DNA in mucosal and genital ulcers in the context of suspected syphilis. In ...

journal_title：Journal of clinical microbiology

authors： Grange PA,Jary A,Isnard C,Burrel S,Boutolleau D,Touati A,Bébéar C,Saule J,Martinet P,Robert JL,Moulene D,Vermersch-Langlin A,Benhaddou N,Janier M,Dupin N

更新日期：2021-01-21 00:00:00