InTray GC medium versus modified Thayer-Martin agar plates for diagnosis of gonorrhea from endocervical specimens.

abstract：:We conducted a Linear Array test/retest analysis using cytologic specimens from 198 women. A total of 67.2% of samples had the same human papillomavirus (HPV) types detected in both tests (type-specific positive agreement was 83.3% overall [Kappa = 0.9] and 86.8% for carcinogenic types [Kappa = 0.92]). Discordance was...

journal_title：Journal of clinical microbiology

authors： Koshiol J,Dunn ST,Walker JL,Zuna RE,Schiffman M,Sherman ME,Gold MA,Allen RA,Zhang R,Wang SS,Wentzensen N

更新日期：2013-02-01 00:00:00

abstract：:The accuracy of the new API YeastIdent system and the Flow Laboratories Uni-Yeast-Tek identification kit with an expanded data base was evaluated in comparison to the API 20C yeast identification system by three laboratories. A total of 489 test isolates were used, biased toward yeasts commonly encountered in clinical...

journal_title：Journal of clinical microbiology

authors： Salkin IF,Land GA,Hurd NJ,Goldson PR,McGinnis MR

更新日期：1987-04-01 00:00:00

abstract：:Nonfermenting bacteria are ubiquitous environmental opportunists that cause infections in humans, especially compromised patients. Due to their limited biochemical reactivity and different morphotypes, misidentification by classical phenotypic means occurs frequently. Therefore, we evaluated the use of matrix-assisted...

journal_title：Journal of clinical microbiology

authors： Mellmann A,Cloud J,Maier T,Keckevoet U,Ramminger I,Iwen P,Dunn J,Hall G,Wilson D,Lasala P,Kostrzewa M,Harmsen D

更新日期：2008-06-01 00:00:00

abstract：:We used an enzyme-linked immunosorbent assay (ELISA) of immunoglobulin G avidity to determine the dengue immune status of 105 pairs of serum samples from patients infected with dengue virus. This study shows that a simple avidity test, for which only one acute-phase serum sample is required, is potentially more useful...

journal_title：Journal of clinical microbiology

authors： Matheus S,Deparis X,Labeau B,Lelarge J,Morvan J,Dussart P

更新日期：2005-11-01 00:00:00

abstract：:The increased prevalence and the severity of oropharyngeal candidiasis in human immunodeficiency virus (HIV)-positive patients are attributed exclusively to the virus-induced immune deficiency of the host. The present study was aimed at answering the question of whether Candida albicans secretory proteinase, a putativ...

journal_title：Journal of clinical microbiology

authors： Ollert MW,Wende C,Görlich M,McMullan-Vogel CG,Borg-von Zepelin M,Vogel CW,Korting HC

更新日期：1995-10-01 00:00:00

abstract：:Human adenoviruses (AdVs) typically cause mild illnesses in otherwise healthy hosts. We investigated a pediatric outbreak of acute respiratory infection with fatal outcomes that occurred in Lisbon, Portugal, in 2004. Biological specimens were collected from 83 children attending two nurseries, a kinesiotherapy clinic,...

journal_title：Journal of clinical microbiology

authors： Rebelo-de-Andrade H,Pereira C,Gíria M,Prudêncio E,Brito MJ,Calé E,Taveira N

更新日期：2010-04-01 00:00:00

abstract：:The Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) provides a national service for the laboratory confirmation of meningococcal and pneumococcal disease in Scotland. Part of this service includes the serogrouping of meningococcal isolates followed by typing and subtyping. The procedures for this ...

journal_title：Journal of clinical microbiology

authors： Clarke SC,Diggle MA,Edwards GF

更新日期：2001-09-01 00:00:00

abstract：:Several media were used to evaluate the hemolytic activity of Listeria monocytogenes, and this property was used along with the API Listeria system to identify Listeria spp. All L. monocytogenes strains were identified correctly with this system, and blood agar base no. 2 and Columbia blood agar base supplemented with...

journal_title：Journal of clinical microbiology

authors： Fujisawa T,Mori M

更新日期：1994-04-01 00:00:00

abstract：:A high-performance liquid chromatography method that utilized fluorescence detection (HPLC-FL) of mycolic acid 6,7-dimethoxycoumarin esters was developed to identify Mycobacterium tuberculosis (MTB) and M. avium complex (MAC) directly from fluorochrome stain smear-positive sputum specimens and young BACTEC 12B culture...

journal_title：Journal of clinical microbiology

authors： Jost KC Jr,Dunbar DF,Barth SS,Headley VL,Elliott LB

更新日期：1995-05-01 00:00:00

abstract：:Streptococcus pneumoniae plays an important role in causing acute exacerbations in patients with chronic respiratory disease. However, few data are available regarding pneumococcal persistence in adult patients with chronic respiratory diseases. Fifty pneumococci recovered from sputum samples (1995 to 2010) from 13 ad...

journal_title：Journal of clinical microbiology

authors： Domenech A,Ardanuy C,Balsalobre L,Marti S,Calatayud L,De la Campa AG,Brueggemann AB,Liñares J

更新日期：2012-12-01 00:00:00

abstract：:Vaginal discharge specimens from 105 women were examined by wet mount, culture, and a new direct immunofluorescence assay to detect Trichomonas vaginalis. The organism was detected by culture in 31 patients, by direct immunofluorescence in 26 patients, and by wet mount in 21 patients. ...

journal_title：Journal of clinical microbiology

authors： Smith RF

更新日期：1986-12-01 00:00:00

abstract：:Testing for susceptibility to pyrazinamide (PZA) and analysis of the pncA gene sequences of 423 Mycobacterium tuberculosis complex isolates have revealed a unique silent nucleotide substitution that enables the rapid identification of "M. canettii" (proposed name). Moreover, the lack of a defined mutation within the p...

journal_title：Journal of clinical microbiology

authors： Somoskovi A,Dormandy J,Parsons LM,Kaswa M,Goh KS,Rastogi N,Salfinger M

更新日期：2007-02-01 00:00:00

abstract：:Automated DNA sequencing was used to characterize mutations associated with rifampin resistance in a 69-bp region of the gene, rpoB, encoding the beta subunit of RNA polymerase in Mycobacterium tuberculosis. The data confirmed that greater than 90% of rifampin-resistant strains have sequence alterations in this region...

journal_title：Journal of clinical microbiology

authors： Kapur V,Li LL,Iordanescu S,Hamrick MR,Wanger A,Kreiswirth BN,Musser JM

更新日期：1994-04-01 00:00:00

abstract：:Branched DNA (bDNA) assays to quantify human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) consist of three distinct steps, including sample processing, hybridization, and detection, and utilize the System 340 platform for plate incubation and washing. Sample processing differs: HIV-1 from 1 ml of ...

journal_title：Journal of clinical microbiology

authors： Elbeik T,Markowitz N,Nassos P,Kumar U,Beringer S,Haller B,Ng V

更新日期：2004-07-01 00:00:00

abstract：:Ninety-four pharyngeal swab samples corresponding to 94 patients with suspected influenza virus infection were inoculated in Madin-Darby canine kidney (MDCK) cells, the conventional cell system for the isolation of influenza virus, and in fibroblastic human embryo lung (MRC-5) cells, a cell system less commonly used f...

journal_title：Journal of clinical microbiology

authors： de Oña M,Melón S,de la Iglesia P,Hidalgo F,Verdugo AF

更新日期：1995-07-01 00:00:00

abstract：:The sensitivities and specificities of three immunoassays for the detection of herpes simplex virus type 2 (HSV-2)-specific immunoglobulin G antibodies in serum, including the one-strip rapid immunoblot assay (RIBA; Chiron Corporation) and two indirect enzyme immunosorbent assays (EIA; Gull Laboratories and Centocor),...

journal_title：Journal of clinical microbiology

authors： Groen J,Van Dijk G,Niesters HG,Van Der Meijden WI,Osterhaus AD

更新日期：1998-03-01 00:00:00

abstract：:The Lyme disease-associated spirochete, Borrelia burgdorferi, is maintained in enzootic cycles involving Ixodes ticks and small mammals. Previous studies demonstrated that B. burgdorferi expresses outer surface protein A (OspA) but not OspC when residing in the midgut of unfed ticks. However, after ticks feed on blood...

journal_title：Journal of clinical microbiology

authors： Schwan TG,Piesman J

更新日期：2000-01-01 00:00:00

abstract：:Data are limited for the performance of enzyme immunoassays for the detection of Chlamydia trachomatis in conjunctival and nasopharyngeal specimens from infants. The only available data are for one assay, Chlamydiazyme (Abbott Diagnostics). The purpose of this study was to compare a new enzyme immunoassay, Pathfinder ...

journal_title：Journal of clinical microbiology

authors： Hammerschlag MR,Roblin PM,Gelling M,Worku M

更新日期：1990-08-01 00:00:00

abstract：:Two rapid diagnostic tests for Chlamydia trachomatis (Microtrak, Syva Co., Palo Alto, Calif.; and Chlamydiazyme, Abbott Laboratories, North Chicago, Ill.) were evaluated in comparison with growth of the organism in tissue culture for 2,030 urogenital specimens from men and women. Neither test performed as well as cult...

journal_title：Journal of clinical microbiology

authors： Hipp SS,Han Y,Murphy D

更新日期：1987-10-01 00:00:00

abstract：:A rapid screening test has been developed to detect heat-labile enterotoxin produced by individual colonies of Escherichia coli or Vibrio cholerae growing on media solidified with agar. The applicability of this method for isolating tox mutants of E. coli and V. cholerae has been demonstrated. ...

journal_title：Journal of clinical microbiology

authors： Bramucci MG,Holmes RK

更新日期：1978-08-01 00:00:00

abstract：:Rodent models have been developed to study the pathogenesis of diseases caused by Helicobacter pylori, as well as by other gastric and intestinal Helicobacter spp., but some murine enteric Helicobacter spp. cause hepatobiliary and intestinal tract diseases in specific inbred strains of laboratory mice. To identify the...

journal_title：Journal of clinical microbiology

authors： Nilsson HO,Ouis IS,Stenram U,Ljungh A,Moran AP,Wadström T,Al-Soud WA

更新日期：2004-08-01 00:00:00

abstract：:Leishmania (Viannia) braziliensis causes three main types of American tegumentary leishmaniasis (ATL), localized cutaneous leishmaniasis (CL), mucosal leishmaniasis (ML), and disseminated leishmaniasis (DL). All forms are observed among individuals of Corte de Pedra, Brazil. We previously used random amplified markers...

journal_title：Journal of clinical microbiology

authors： Queiroz A,Sousa R,Heine C,Cardoso M,Guimarães LH,Machado PR,Carvalho EM,Riley LW,Wilson ME,Schriefer A

更新日期：2012-12-01 00:00:00

abstract：:Immunoglobulin M (IgM) and IgA antibodies against the major surface protein of Toxoplasma gondii were determined in a total of 195 human sera and five human cerebrospinal fluids by using a P30 membrane extract and the immunoblot technique. By using two different T. gondii strains (RH and BK) simultaneously as antigens...

journal_title：Journal of clinical microbiology

authors： Gross U,Roos T,Appoldt D,Heesemann J

更新日期：1992-06-01 00:00:00

abstract：:The Enzygnost alpha method was tested against the complement fixation test and anti-VCA immunofluorescence to determine the respective titers of anti-cytomegalovirus and anti-Epstein-Barr virus immunoglobulin G antibodies. For cytomegalovirus, the Enzygnost results showed 97.99% agreement with the readings obtained by...

journal_title：Journal of clinical microbiology

authors： Gutierrez J,Maroto MD,Piédrola G

更新日期：1994-10-01 00:00:00

abstract：:Of 90 clinical isolates of Cryptococcus neoformans studied, 3 were determined to be serotype B/C. The patients from whom these B/C isolates were obtained were identified as never having lived in or visited the areas associated with B/C serotypes. This finding suggests a broader geographic distribution of this serotype...

journal_title：Journal of clinical microbiology

authors： Fromtling RA,Shadomy S,Shadomy HJ,Dismukes WE

更新日期：1982-08-01 00:00:00

abstract：:Plasmids encoding extended-spectrum beta-lactamases of the TEM, SHV, and AmpC families were introduced into common Escherichia coli and Klebsiella pneumoniae hosts to create a homogeneous panel for evaluating the abilities of five test systems to detect resistance to eight beta-lactam antibiotics. Although MICs, as de...

journal_title：Journal of clinical microbiology

authors： Katsanis GP,Spargo J,Ferraro MJ,Sutton L,Jacoby GA

更新日期：1994-03-01 00:00:00

abstract：:Immune pressure on lentiviruses exerted by cytotoxic T lymphocytes (CTL) selects for virus CTL epitope mutations. Currently employed methods for monitoring emerging CTL epitope mutations rely on the labor-intensive and time-consuming techniques of virus population or clonal sequencing. Here we describe the development...

journal_title：Journal of clinical microbiology

authors： Peyerl FW,Barouch DH,Bazick HS,Manuel E,Letvin NL

更新日期：2005-09-01 00:00:00

abstract：:We used genotypic and phenotypic assays to estimate the frequency of X4/DM viruses in 131 patients infected with non-subtype-B viruses at the time of primary HIV-1 infection (PHI). All patients were enrolled in the French PRIMO Cohort from 1996 to 2007. Most strains belonged to CRF02_AG (51.1%) and subtype A (14.5%). ...

journal_title：Journal of clinical microbiology

authors： Frange P,Chaix ML,Raymond S,Galimand J,Deveau C,Meyer L,Goujard C,Rouzioux C,Izopet J,French ANRS CO 06 PRIMO Cohort Study Group.

更新日期：2010-10-01 00:00:00

abstract：:A mathematical analysis of serological data has provided the basis for a new serotyping routine. This paper describes the process whereby the results of agglutination reactions between the standard O antigens of Escherichia coli and rabbit antisera raised against them were used to produce a much simpler, faster, and c...

journal_title：Journal of clinical microbiology

authors： Thompson CJ

更新日期：1987-05-01 00:00:00

abstract：:Currently, no agar-based susceptibility testing method has been standardized for testing dermatophytes. We describe a newly developed agar-based method employing disk diffusion assay to test the susceptibility of 47 isolates of dermatophytes against 8 antifungals. Our results show that the method is reproducible, is s...

journal_title：Journal of clinical microbiology

authors： Nweze EI,Mukherjee PK,Ghannoum MA

更新日期：2010-10-01 00:00:00