Agar-based disk diffusion assay for susceptibility testing of dermatophytes.

abstract：:Evaluations of screening tests for bacteriuria have traditionally compared the test results with those of quantitative urine cultures. However, many patients with symptomatic urinary tract infections can have less than 10(5) CFU/ml in their urine. Therefore, the results of urine culture and three screening tests (Bac-...

journal_title：Journal of clinical microbiology

authors： Murray PR,Smith TB,McKinney TC Jr

更新日期：1987-03-01 00:00:00

abstract：:Detection of Staphylococcus aureus isolates with intermediate vancomycin susceptibility (VISA) and heteroresistance (hVISA) remains problematic. The population analysis profile/area under the curve (PAP/AUC) is the gold standard but is cumbersome. We compared the performance of two Etest screening methods (macromethod...

journal_title：Journal of clinical microbiology

authors： Riederer K,Shemes S,Chase P,Musta A,Mar A,Khatib R

更新日期：2011-06-01 00:00:00

abstract：:Two bacteriologic systems for detecting bacteria in blood were compared; the automated radiometric BACTEC and the conventional method used in our laboratory for many years. BACTEC consisted of two bottles with 30 ml and the conventional method with 50 ml of media for aerobes and anaerobes. The BACTEC bottles were inoc...

journal_title：Journal of clinical microbiology

authors： Thiemke WA,Wicher K

更新日期：1975-03-01 00:00:00

abstract：:Fresh urine pellets from human immunodeficiency virus type 1 (HIV-1)-seropositive individuals were examined for the presence of the HIV-1 genomic sequence and gene products. By using the polymerase chain reaction technique, HIV-1 DNA proviral sequences were detected in 53 of 80 (66.25%) fresh urine pellets from HIV-1-...

journal_title：Journal of clinical microbiology

authors： Li JJ,Huang YQ,Poiesz BJ,Zaumetzger-Abbot L,Friedman-Kien AE

更新日期：1992-05-01 00:00:00

abstract：:Four human coronaviruses (HCoV-229E, HCoV-HKU1, HCoV-NL63, and HCoV-OC43) are associated with a range of respiratory outcomes, including bronchiolitis and pneumonia. Their epidemiologies and clinical characteristics are poorly described and are often reliant on case reports. To address these problems, we conducted a l...

journal_title：Journal of clinical microbiology

authors： Gaunt ER,Hardie A,Claas EC,Simmonds P,Templeton KE

更新日期：2010-08-01 00:00:00

abstract：:We explored three genes of attachment (fbe and atlE) and adhesion (ica) in 27 and 10 Staphylococcus epidermidis strains involved in pacemaker-related infections (PMI) and intravascular-catheter-related infections (IVCI), respectively, and in 25 saprophytic strains. The detection rates of fbe and atlE were identical in...

journal_title：Journal of clinical microbiology

authors： Klug D,Wallet F,Kacet S,Courcol RJ

更新日期：2003-07-01 00:00:00

abstract：:A cluster of methicillin-resistant Staphylococcus aureus (MRSA) infections among patients on an intensive care unit (ICU) was detected by routine infection control surveillance. In the period from 5 January to 22 June 1995, 10 patients on the ICU and a further 6 patients (5 on one ward that had received colonized pati...

journal_title：Journal of clinical microbiology

authors： Tambic A,Power EG,Talsania H,Anthony RM,French GL

更新日期：1997-12-01 00:00:00

abstract：:The new colorimetric Vitek 2 YST card was evaluated for identification of yeasts (136 strains) with respect to the influence of different source media. The Vitek 2 YST card achieved satisfactory results for all yeast species tested, with the exception of Candida guilliermondii, Candida norvegensis, Candida parapsilosi...

journal_title：Journal of clinical microbiology

authors： Valenza G,Strasen J,Schäfer F,Frosch M,Kurzai O,Abele-Horn M

更新日期：2008-11-01 00:00:00

abstract：:We evaluated three rapid identification systems-The Biomerieux rapid ID 32 STREP (ID32), the BBL Crystal rapid gram-positive identification (Crystal), and the Remel IDS RapID STR (IDS) systems-for their ability to identify 7 strains of Alloiococcus otitidis, 27 strains of Dolosigranulum pigrum, 3 strains of Ignavigran...

journal_title：Journal of clinical microbiology

authors： LaClaire LL,Facklam RR

更新日期：2000-06-01 00:00:00

abstract：:Clostridium difficile is now recognized as the major agent responsible for nosocomial diarrhea in adults. Among the genotyping methods available, arbitrarily primed PCR (AP-PCR), PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) have been widely used for investigating outbreaks of C. difficile infections. Ho...

journal_title：Journal of clinical microbiology

authors： Bidet P,Lalande V,Salauze B,Burghoffer B,Avesani V,Delmée M,Rossier A,Barbut F,Petit JC

更新日期：2000-07-01 00:00:00

abstract：:A high-performance liquid chromatography method that utilized fluorescence detection (HPLC-FL) of mycolic acid 6,7-dimethoxycoumarin esters was developed to identify Mycobacterium tuberculosis (MTB) and M. avium complex (MAC) directly from fluorochrome stain smear-positive sputum specimens and young BACTEC 12B culture...

journal_title：Journal of clinical microbiology

authors： Jost KC Jr,Dunbar DF,Barth SS,Headley VL,Elliott LB

更新日期：1995-05-01 00:00:00

abstract：:Evaluation of optimal compositions of blood culture media has called for extensive and laborious work in comparative studies of large series of clincal specimens. Bacterial growth is accompanied by heat production, and calorimetry provides an analytical tool for its detection and quantification. A twin microcalorimete...

journal_title：Journal of clinical microbiology

authors： Ripa KT,Mårdh PA,Hovelius B,Ljungholm K

更新日期：1977-04-01 00:00:00

abstract：:We developed a loop-mediated isothermal amplification method able to detect Yersinia pseudotuberculosis strains in 30 min by using six primers designed by targeting the inv gene. This method is more sensitive than PCR and might be a useful tool for detecting and identifying Y. pseudotuberculosis. ...

journal_title：Journal of clinical microbiology

authors： Horisaka T,Fujita K,Iwata T,Nakadai A,Okatani AT,Horikita T,Taniguchi T,Honda E,Yokomizo Y,Hayashidani H

更新日期：2004-11-01 00:00:00

abstract：:Rapid identification and typing of methicillin (meticillin)-resistant Staphylococcus aureus (MRSA) is important for understanding the molecular epidemiology and evolution of MRSA and offers many advantages for controlling transmission in both health care and community settings. We developed a rapid molecular beacon re...

journal_title：Journal of clinical microbiology

authors： Chen L,Mediavilla JR,Oliveira DC,Willey BM,de Lencastre H,Kreiswirth BN

更新日期：2009-11-01 00:00:00

abstract：:The sensitivities of BHK-21 (C-13) and murine neuroblastoma (C-1300; clone NA) cells for the isolation of small quantities of a street strain rabies virus were compared. Suspensions of brain from mice sacrificed prior to the onset of clinical signs of rabies were used to stimulate weakly positive diagnostic specimens....

journal_title：Journal of clinical microbiology

authors： Rudd RJ,Trimarchi CV

更新日期：1987-08-01 00:00:00

abstract：:The global spread of carbapenemase-producing Enterobacteriaceae (CPE) that are often resistant to most, if not all, classes of antibiotics is a major public health concern. The NDM-1 carbapenemase is among the most worrisome carbapenemases given its rapid worldwide spread. We have developed and evaluated a lateral flo...

journal_title：Journal of clinical microbiology

authors： Boutal H,Naas T,Devilliers K,Oueslati S,Dortet L,Bernabeu S,Simon S,Volland H

更新日期：2017-07-01 00:00:00

abstract：:Borrelia burgdorferi sensu lato species display considerable antigenic polymorphism. In order to evaluate the importance of this antigenic heterogeneity in the serodiagnosis of Lyme disease, the serum immunoglobulin G response in 148 healthy individuals from an area in northern Sweden where Lyme disease is endemic and...

journal_title：Journal of clinical microbiology

authors： Bunikis J,Olsén B,Westman G,Bergstroöm S

更新日期：1995-06-01 00:00:00

abstract：:We compared examination of unstained and Gram-stained smears as methods of evaluating sputum quality. Of 100 slides examined, 96 (96%) were graded identically. Each discrepancy between methods was near the cutoff for specimen acceptability. We concluded that examination of unstained smears can provide an accurate, cos...

journal_title：Journal of clinical microbiology

authors： Wetterau LM,Zeimis RT,Hollick GE

更新日期：1986-07-01 00:00:00

abstract：:The AutoSCAN-3 (American MicroScan, Mahwah, N.J.) is an instrument capable of automated reading of commercially available microdilution trays for identification and quantitative susceptibility testing of rapidly growing bacteria. This study compared the results of visual and automated reading of microdilution trays fo...

journal_title：Journal of clinical microbiology

authors： DeGirolami PC,Eichelberger KA,Salfity LC,Rizzo MF

更新日期：1983-12-01 00:00:00

abstract：:We designed a highly sensitive and specific polymerase chain reaction assay for the detection of Epstein-Barr virus (EBV)-related sequences in B-cell DNA of EBV-seropositive healthy individuals. By using this assay, we were able to amplify at least 10 copies of a plasmid containing the BamHI-W region, which is repeate...

journal_title：Journal of clinical microbiology

authors： Wagner HJ,Bein G,Bitsch A,Kirchner H

更新日期：1992-11-01 00:00:00

abstract：:Nontuberculous mycobacterial infections caused by Mycobacterium abscessus are responsible for a range of disease manifestations from pulmonary to skin infections and are notoriously difficult to treat, due to innate resistance to many antibiotics. Previous population studies of clinical M. abscessus isolates utilized ...

journal_title：Journal of clinical microbiology

authors： Davidson RM,Hasan NA,Reynolds PR,Totten S,Garcia B,Levin A,Ramamoorthy P,Heifets L,Daley CL,Strong M

更新日期：2014-10-01 00:00:00

abstract：:Four HIV rapid tests were subjected to field validation in Panama and compared to an enzyme-linked immunosorbent assay/Multispot-based testing algorithm. The sensitivities of Determine, Uni-Gold, SD Bioline, and INSTI were 99.8%. The specificities of Determine, SD-Bioline, and Uni-Gold were 100%, and the specificity o...

journal_title：Journal of clinical microbiology

authors： Juarez SI,Nuñez AE,Aranda MM,Mojica D,Kim AA,Parekh B

更新日期：2016-04-01 00:00:00

abstract：:A supplemented 2% peptone broth is described for the detection of indole production by Pasteurella multocida. The 96 isolates of P. multocida that were utilized in this evaluation were indole positive within 18 to 24 h. ...

journal_title：Journal of clinical microbiology

authors： Clemons KV,Gadberry JL

更新日期：1982-04-01 00:00:00

abstract：:Forty-four nonreplicate clinical isolates of Pseudomonas aeruginosa that were resistant to extended-spectrum cephalosporins (ceftazidime and cefepime) and aztreonam, that putatively produced an acquired extended- spectrum beta-lactamase (ESBL), according to the results of a double-disk synergy test, and that had been ...

journal_title：Journal of clinical microbiology

authors： Pagani L,Mantengoli E,Migliavacca R,Nucleo E,Pollini S,Spalla M,Daturi R,Romero E,Rossolini GM

更新日期：2004-06-01 00:00:00

abstract：:The QuadFERM+ system (Analytab Products, Plainview, N.Y.), a 2-h carbohydrate degradation method for the identification of Neisseria spp., was evaluated along with a rapid DNase test for confirmation of Branhamella catarrhalis. QuadFERM+ identified 100% of 82 N. gonorrhoeae and 96% of 54 N. meningitidis strains. The t...

journal_title：Journal of clinical microbiology

authors： Janda WM,Zigler KL,Bradna JJ

更新日期：1987-02-01 00:00:00

abstract：:The prevalence of class D extended-spectrum oxacillinases (ES-OXAs) in ceftazidime-resistant strains of Pseudomonas aeruginosa is often underestimated by double-disk synergy tests (DDST) using clavulanate. A DDST with a customized distance between a disk of ceftazidime or cefepime and inhibitors (clavulanate and imipe...

journal_title：Journal of clinical microbiology

authors： Hocquet D,Dehecq B,Bertrand X,Plésiat P

更新日期：2011-06-01 00:00:00

abstract：:The Porphyromonas gingivalis collagenase-specific serum immunoglobulin A (IgA), IgM, and IgG responses from 20 patients with early-onset periodontitis (EOP), 20 patients with adult periodontitis, (AP), and 20 age- and sex-matched healthy controls were examined by immunoblot analysis. A recombinant collagenase antigen ...

journal_title：Journal of clinical microbiology

authors： Wittstock M,Flemmig TF,Schmidt H,Mutters R,Karch H

更新日期：1996-10-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) strains display genetic polymorphisms, and these polymorphisms can be analyzed to study viral transmission and pathogenesis. Recently, short tandem repeat (STR) length polymorphisms have been identified in the HCMV genome. We assessed the utility of STRs in characterizing HCMV strains and ...

journal_title：Journal of clinical microbiology

authors： Walker A,Petheram SJ,Ballard L,Murph JR,Demmler GJ,Bale JF Jr

更新日期：2001-06-01 00:00:00

abstract：:Development of resistance during therapy with cefamandole contributes to treatment failure. A simple cefoxitin disk test was recently described which detects a cefamandole-active inducible beta-lactamase not otherwise detectable with cefamandole as the inducer. A case of breakthrough Enterobacter bacteremia due to se...

journal_title：Journal of clinical microbiology

authors： Beckwith DG,Jahre JA

更新日期：1980-10-01 00:00:00

abstract：:We have previously reported that the pCS20 PCR detection assay for Cowdria ruminantium, the causative agent of heartwater disease of ruminants, is more sensitive than xenodiagnosis and the pCS20 DNA probe for the detection of infection in the vector Amblyomma ticks. Here, we further assessed the reliability of the PCR...

journal_title：Journal of clinical microbiology

authors： Peter TF,Barbet AF,Alleman AR,Simbi BH,Burridge MJ,Mahan SM

更新日期：2000-04-01 00:00:00