Abstract:
:Cytomegalovirus (CMV) antigenemia was directly detected in polymorphonuclear leukocytes (PMNLs) from transplant recipients by using flow cytometry (FC). Two fixation and permeabilization methods and seven anti-CMV monoclonal antibodies (MAbs) were evaluated. 1C3, SL20, and NEA-9221 MAbs were more efficacious. The antigenemia detection threshold of FC was 0.05% positive PMNLs, and percentages correlated well with DNA viral load and the appearance of clinical symptoms.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Imbert-Marcille BM,Robillard N,Poirier AS,Coste-Burel M,Cantarovich D,Milpied N,Billaudel Sdoi
10.1128/JCM.35.10.2665-2669.1997subject
Has Abstractpub_date
1997-10-01 00:00:00pages
2665-9issue
10eissn
0095-1137issn
1098-660Xjournal_volume
35pub_type
杂志文章abstract::To facilitate the routine identification of salmonellae and detailed studies of their lipopolysaccharides, we raised murine monoclonal antibodies against these organisms. We raised an immunoglobulin G1 antibody, MO2, which is specific for factor O2. By immunoblotting following sodium dodecyl sulfate-polyacrylamide gel...
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
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更新日期:2013-04-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2000-12-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.15.3.362-365.1982
更新日期:1982-03-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2009-11-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:1996-04-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.16.6.1137-1144.1982
更新日期:1982-12-01 00:00:00
abstract::Characteristics differentiating Escherichia coli strains that cause cystitis or pyelonephritis from fecal E. coli remain incompletely defined, particularly among adult women in the United States. Accordingly, phylogenetic group, O antigens, and virulence factors (VFs) were analyzed among 329 E. coli isolates from the ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2005-12-01 00:00:00