Three strains of influenza A virus (H3N2): interferon sensitivity in vitro and interferon production in volunteers.

abstract：:Campylobacter jejuni from sporadic cases and outbreaks of gastroenteritis were serotyped on the basis of heat-extracted soluble thermostable antigens identified with the use of the passive hemagglutination technique. A total of 168 isolates were separated into 45 different types. The largest proportion of the isolates...

journal_title：Journal of clinical microbiology

authors： McMyne PM,Penner JL,Mathias RG,Black WA,Hennessy JN

更新日期：1982-08-01 00:00:00

abstract：:The effects of Tween 80 supplementation of liquid culture medium on the formation of the superficial L1 layer of the Mycobacterium avium-Mycobacterium intracellulare complex (MAC) were examined by serological and scanning electron microscopic experiments. Specific antiserum to the glycopeptidolipids on the L1 layer of...

journal_title：Journal of clinical microbiology

authors： Masaki S,Sugimori G,Okamoto A,Imose J,Hayashi Y

更新日期：1991-07-01 00:00:00

abstract：:A total of 101 food-borne and waterborne outbreaks that were caused by norovirus and that resulted in more than 4,100 cases of illness were reported to the Swedish Institute for Infectious Disease Control from January 2002 to December 2006. Sequence and epidemiological data for isolates from 73 outbreaks were analyzed...

journal_title：Journal of clinical microbiology

authors： Lysén M,Thorhagen M,Brytting M,Hjertqvist M,Andersson Y,Hedlund KO

更新日期：2009-08-01 00:00:00

abstract：:The babA2 and cagA genes were investigated in 208 Brazilian Helicobacter pylori strains. A strong association between babA2 and duodenal ulcer or gastric carcinoma was observed, even after adjusting for confounding factors, such as age, gender, and cagA status. cagA-positive strains were also independently associated ...

journal_title：Journal of clinical microbiology

authors： Oliveira AG,Santos A,Guerra JB,Rocha GA,Rocha AM,Oliveira CA,Cabral MM,Nogueira AM,Queiroz DM

更新日期：2003-08-01 00:00:00

abstract：:A new PCR-reverse dot blot hybridization (RDBH) assay was developed for the rapid identification of Mycobacterium species in clinical isolates. The assay, which targets the 16S rRNA, was evaluated for 27 mycobacterial reference strains and 340 clinical isolates that were simultaneously identified by DNA sequencing and...

journal_title：Journal of clinical microbiology

authors： Wu X,Zhang J,Liang J,Lu Y,Li H,Li C,Yue J,Zhang L,Liu Z

更新日期：2007-06-01 00:00:00

abstract：:The incidence of antimicrobial-resistant Neisseria gonorrhoeae continues to rise in Canada; however, antimicrobial resistance data are lacking for approximately 70% of gonorrhea infections that are diagnosed directly from clinical specimens by nucleic acid amplification tests (NAATs). We developed a molecular assay fo...

journal_title：Journal of clinical microbiology

authors： Peterson SW,Martin I,Demczuk W,Bharat A,Hoang L,Wylie J,Allen V,Lefebvre B,Tyrrell G,Horsman G,Haldane D,Garceau R,Wong T,Mulvey MR

更新日期：2015-07-01 00:00:00

abstract：:Fimbriae of Porphyromonas gingivalis are filamentous appendages on the cell surface and are thought to be one of the virulence factors. The fimA gene encoding the subunit protein of fimbriae, fimbrillin (FimA), was classified into four typeable variants (types I to IV). We previously examined the distribution of P. gi...

journal_title：Journal of clinical microbiology

authors： Nakagawa I,Amano A,Kimura RK,Nakamura T,Kawabata S,Hamada S

更新日期：2000-05-01 00:00:00

abstract：:The 1896 precore (PC) mutation is the most frequent cause of hepatitis B virus e-antigen (HBeAg)-negative chronic hepatitis B virus (HBV) infection. Detection of the 1896 PC mutation has application in studies monitoring antiviral therapy and the natural history of the disease. Identification of this mutation is usual...

journal_title：Journal of clinical microbiology

authors： Waltz TL,Marras S,Rochford G,Nolan J,Lee E,Melegari M,Pollack H

更新日期：2005-01-01 00:00:00

abstract：:Rapid identification and typing of methicillin (meticillin)-resistant Staphylococcus aureus (MRSA) is important for understanding the molecular epidemiology and evolution of MRSA and offers many advantages for controlling transmission in both health care and community settings. We developed a rapid molecular beacon re...

journal_title：Journal of clinical microbiology

authors： Chen L,Mediavilla JR,Oliveira DC,Willey BM,de Lencastre H,Kreiswirth BN

更新日期：2009-11-01 00:00:00

abstract：:A real-time quantitative PCR assay has been developed to measure human herpesvirus 6 (HHV-6) DNA in biological specimens. The assay sensitivity was 10 copies of DNA per well, with a linear dynamic range of 10 to 10(7) copies of HHV-6 DNA. Intra- and interassay variations were, respectively, 0.88 and 0.8% for samples c...

journal_title：Journal of clinical microbiology

authors： Collot S,Petit B,Bordessoule D,Alain S,Touati M,Denis F,Ranger-Rogez S

更新日期：2002-07-01 00:00:00

abstract：:DNA colony hybridization with three 18- to 20-base-long synthetic oligonucleotide probes for cholera toxin (CT) was used to screen 12 clinical isolates of Aeromonas hydrophila. Under stringent hybridizing (overnight at 40 degrees C) and washing (1 h at 50 degrees C) conditions, nine strains reacted with the 32P-labele...

journal_title：Journal of clinical microbiology

authors： Schultz AJ,McCardell BA

更新日期：1988-01-01 00:00:00

abstract：:Shortly after adopting a 6-week-old cat, a veterinarian was bitten on the left index finger. Within 3 weeks, he developed headache, fever, and left axillary lymphadenopathy. Initial blood cultures from the cat and veterinarian were sterile. Repeat cultures from the cat grew Bartonella-like organisms with lophotrichous...

journal_title：Journal of clinical microbiology

authors： Kordick DL,Hilyard EJ,Hadfield TL,Wilson KH,Steigerwalt AG,Brenner DJ,Breitschwerdt EB

更新日期：1997-07-01 00:00:00

abstract：:Febrile travelers from countries with unique endemic pathogens pose a significant diagnostic challenge. In this report, we describe the case of a Tongan man presenting with fever, rash, and altered mental status. The diagnosis of Chikungunya encephalitis was made using a laboratory-developed real-time RT-PCR and serol...

journal_title：Journal of clinical microbiology

authors： Nelson J,Waggoner JJ,Sahoo MK,Grant PM,Pinsky BA

更新日期：2014-09-01 00:00:00

abstract：:Recently, three novel avian-origin swine influenza viruses (SIVs) were first isolated from pigs in Guangdong Province, southern China, yet little is known about the seroprevalence of avian influenza viruses among pigs in southern China. Here, we report for the first time the seroprevalence of avian H3, H4, and H6 infl...

journal_title：Journal of clinical microbiology

authors： Su S,Qi W,Chen J,Zhu W,Huang Z,Xie J,Zhang G

更新日期：2013-02-01 00:00:00

abstract：:The sequencing of the VP1 hypervariable region of the human enterovirus (HEV) genome has become the reference test for typing field isolates. This study describes a new strategy for typing HEV at the serotype level that uses a reverse transcription-PCR assay targeting the central part of the VP2 capsid protein. Two pa...

journal_title：Journal of clinical microbiology

authors： Nasri D,Bouslama L,Omar S,Saoudin H,Bourlet T,Aouni M,Pozzetto B,Pillet S

更新日期：2007-08-01 00:00:00

abstract：:A procedure for extracting rotaviral double-stranded ribonucleic acid (RNA) directly from fecal and intestinal specimens collected from calves and pigs is described. This procedure provides a rapid, simple, reproducible method of obtaining rotaviral double-stranded RNA preparations suitable for electrophoretic analysi...

journal_title：Journal of clinical microbiology

authors： Theil KW,McCloskey CM,Saif LJ,Redman DR,Bohl EH,Hancock DD,Kohler EM,Moorhead PD

更新日期：1981-09-01 00:00:00

abstract：:A reverse transcription-PCR (RT-PCR) assay based on automated fluorescent capillary electrophoresis and GeneScan software analysis was developed to detect six common respiratory viruses in clinical specimens from young children. Assays for human respiratory syncytial virus (HRSV); human parainfluenza viruses 1, 2, and...

journal_title：Journal of clinical microbiology

authors： Erdman DD,Weinberg GA,Edwards KM,Walker FJ,Anderson BC,Winter J,González M,Anderson LJ

更新日期：2003-09-01 00:00:00

abstract：:The first World Health Organization (WHO) international standards (ISs) for nucleic acid amplification techniques were established two decades ago, with the initial focus on blood screening for three major viral targets, i.e., hepatitis C virus, hepatitis B virus, and human immunodeficiency virus 1. These reference ma...

journal_title：Journal of clinical microbiology

authors： Baylis SA,Wallace P,McCulloch E,Niesters HGM,Nübling CM

更新日期：2019-01-02 00:00:00

abstract：:Diagnosis of invasive aspergillosis (IA) is still a major problem in routine clinical practice. Early diagnosis is essential for a good patient prognosis. PCR is a highly sensitive method for the detection of nucleic acids and could play an important role in improving the diagnosis of fungal infections. Therefore, a n...

journal_title：Journal of clinical microbiology

authors： Springer J,Schloßnagel H,Heinz W,Doedt T,Soeller R,Einsele H,Loeffler J

更新日期：2012-08-01 00:00:00

abstract：:Tubercle bacilli may survive in unstained heat-fixed sputum smears and may be an infection risk to laboratory staff. We compared the effectiveness of 1% and 5% sodium hypochlorite, 5% phenol, 2% glutaraldehyde, and 3.7% formalin in killing Mycobacterium tuberculosis present in smears prepared from 51 sputum samples. T...

journal_title：Journal of clinical microbiology

authors： Giacomelli LR,Helbel C,Ogassawara RL,Barreto AM,Martins FM,Cardoso CL,Leite CQ

更新日期：2005-08-01 00:00:00

abstract：:Detection of residual plasma viremia in antiretroviral therapy (ART)-suppressed HIV-infected individuals is critical for characterizing the latent reservoir and evaluating the impact of cure interventions. Ultracentrifugation-based single-copy assays are sensitive but labor intensive. Fully automated replicate testing...

journal_title：Journal of clinical microbiology

authors： Bakkour S,Deng X,Bacchetti P,Grebe E,Montalvo L,Worlock A,Stone M,Deeks SG,Richman DD,Busch MP

更新日期：2020-11-18 00:00:00

abstract：:Although substantial epidemiologic evidence links Streptococcus mutans to caries, the pathobiology of caries may involve more complex communities of bacterial species. Molecular methods for bacterial identification and enumeration now make it possible to more precisely study the microbiota associated with dental carie...

journal_title：Journal of clinical microbiology

authors： Becker MR,Paster BJ,Leys EJ,Moeschberger ML,Kenyon SG,Galvin JL,Boches SK,Dewhirst FE,Griffen AL

更新日期：2002-03-01 00:00:00

abstract：:Recent studies have speculated on the possible role of the mother in transmitting Helicobacter pylori infection to their children. In an attempt to either prove or disprove this supposition, we investigated the rates of infection of children born to H. pylori-positive mothers from birth to 5 years of age using serolog...

journal_title：Journal of clinical microbiology

authors： Konno M,Fujii N,Yokota S,Sato K,Takahashi M,Sato K,Mino E,Sugiyama T

更新日期：2005-05-01 00:00:00

abstract：:A new serological assay, the recombinant flow cytometric immunofluorescence assay (r-FIFA), was developed for the early detection of human immunodeficiency virus type 1 (HIV-1) antibodies by using recombinant insoluble forms of HIV-1 Gag-p45, Gag-gp41 chimeric protein, gp160, Po197 polyprotein as antigens and autologo...

journal_title：Journal of clinical microbiology

authors： Hu YW,Birch P,Balaskas E,Zeibdawi A,Scalia V,Thériault-Valin SA,Gill P,Aye MT

更新日期：1996-06-01 00:00:00

abstract：:The low incidence of group B streptococcal (GBS) invasive neonatal disease in Mexico has been attributed to the low prevalence of serotype III strains, a major serotype in developed countries. In addition, nontypeable strains account for 12% of the isolates in Mexico and < 1% of the isolates in the United States. In t...

journal_title：Journal of clinical microbiology

authors： Palacios GC,Eskew EK,Solorzano F,Mattingly SJ

更新日期：1997-11-01 00:00:00

abstract：:Clostridium hathewayi is a newly described Clostridium species isolated from the feces of healthy human individuals, but its clinical significance is not known. We describe a case of human infection associated with C. hathewayi. The bacterium (strain HKU18) was isolated from the blood culture of a 39-year-old patient ...

journal_title：Journal of clinical microbiology

authors： Woo PC,Lau SK,Woo GK,Fung AM,Yiu VP,Yuen KY

更新日期：2004-12-01 00:00:00

abstract：:Chronic hepatitis B virus (HBV) infection via perinatal transmission is common in the Asia-Pacific region, but related quasispecies (QS) characteristics are not yet defined. To investigate the homologous, full-length HBV QS after perinatal infection and their clinical implications, five pairs of mother-daughter patien...

journal_title：Journal of clinical microbiology

authors： Liu F,Yu DM,Huang SY,Yu JL,Zhang DH,Gong QM,Zhang XX

更新日期：2014-05-01 00:00:00

abstract：:Microarrays of virus-specific oligonucleotides may provide a method of screening samples for the presence or absence of a large variety of viruses simultaneously. Influenza viruses are ideal for evaluating such microarrays because of their genetic and host diversity, and the availability of an extensive sequence datab...

journal_title：Journal of clinical microbiology

authors： Sengupta S,Onodera K,Lai A,Melcher U

更新日期：2003-10-01 00:00:00

abstract：:We describe two cases of Clostridium glycolicum wound infections in immunocompetent adults. The bacterium was identified by 16S rRNA gene sequencing. This is the third published report of the recovery of this organism from human clinical material and highlights the importance of the organism as a potential human patho...

journal_title：Journal of clinical microbiology

authors： Jiang W,Abrar S,Romagnoli M,Carroll KC

更新日期：2009-05-01 00:00:00

abstract：:We evaluated the performance of three commercially available monoclonal antibodies for confirmation of the presence of Chlamydia pneumoniae in cell culture by examining their abilities to stain inclusions of eight strains of C. pneumoniae. The antibodies tested were two unconjugated C. pneumoniae-specific monoclonal r...

journal_title：Journal of clinical microbiology

authors： Montalban GS,Roblin PM,Hammerschlag MR

更新日期：1994-05-01 00:00:00