Abstract:
:Spontaneously occurring urinary tract infection (UTI) in dogs was exploited as an experiment of nature to gain insights into UTI pathogenesis in humans. Concurrent urinary and rectal Escherichia coli isolates from 37 dogs with UTI were compared with respect to phylogenetic background, O antigens, and extended virulence genotype. In 54% of the UTI episodes, the dog's urinary and rectal isolates represented the same strain. Urinary isolates differed dramatically from rectal-only isolates in that they derived predominantly from E. coli phylogenetic group B2, expressed typical (human) UTI-associated O antigens, and possessed many virulence-associated genes, most notably pap elements (P fimbriae), papG (adhesin) allele III, sfa/foc and sfaS (S fimbriae), hly (hemolysin), fyuA (yersiniabactin), iroN (siderophore), and ompT (outer membrane protease T). The 20 urinary isolates that corresponded with the host's predominant rectal strain were no less virulent according to the markers analyzed than were the 17 urinary isolates that differed from the host's predominant rectal strain. These findings suggest that UTI pathogenesis is similar in dogs and humans, provide added support for the special-pathogenicity over the prevalence hypothesis of UTI pathogenesis, and identify numerous specific virulence-associated factors as significant correlates of urovirulence.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Johnson JR,Kaster N,Kuskowski MA,Ling GVdoi
10.1128/jcm.41.1.337-345.2003subject
Has Abstractpub_date
2003-01-01 00:00:00pages
337-45issue
1eissn
0095-1137issn
1098-660Xjournal_volume
41pub_type
杂志文章abstract::Continuous cell lines were tested for their potential use in distinguishing pathogenic from nonpathogenic Listeria species. Listeria monocytogenes and Listeria ivanovii strains were lethal for mice, and culture filtrates were cytotoxic for cultured cells and hemolytic for sheep erythrocytes, while Listeria innocua, Li...
journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.25.8.1463-1466.1987
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abstract::More than 700 bacterial species or phylotypes, of which over 50% have not been cultivated, have been detected in the oral cavity. Our purposes were (i) to utilize culture-independent molecular techniques to extend our knowledge on the breadth of bacterial diversity in the healthy human oral cavity, including not-yet-c...
journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.43.11.5721-5732.2005
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abstract::The DNA hybridization assay for genes encoding for Escherichia coli enterotoxins was used to examine water specimens in Thailand. In a reconstruction experiment, the DNA hybridization assay was 10(4) times more sensitive than testing random E. coli in the Y-1 adrenal and suckling mouse assays in identifying enterotoxi...
journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.16.6.1086-1090.1982
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journal_title:Journal of clinical microbiology
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更新日期:1988-09-01 00:00:00
abstract::In a survey of bloodstream infection (BSI) isolates across the continental United States, 162 Candida albicans isolates were fingerprinted with the species-specific probe Ca3 and the patterns were analyzed for relatedness with a computer-assisted system. The results demonstrate that particular BSI strains are more hig...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.36.6.1518-1529.1998
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.39.6.2280-2282.2001
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.1.24-27.1994
更新日期:1994-01-01 00:00:00
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pub_type: 临床试验,杂志文章
doi:10.1128/JCM.35.10.2695-2697.1997
更新日期:1997-10-01 00:00:00
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.38.12.4394-4401.2000
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.6.2554-2559.2003
更新日期:2003-06-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.20.2.300-301.1984
更新日期:1984-08-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
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更新日期:2016-09-01 00:00:00
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.42.11.5385-5387.2004
更新日期:2004-11-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.7.3348-3350.2003
更新日期:2003-07-01 00:00:00
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.26.4.750-754.1988
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.7.3537-3539.2005
更新日期:2005-07-01 00:00:00
abstract::Clinical presentations for viral respiratory tract infections are often nonspecific, and a rapid, high-throughput laboratory technique that can detect a panel of common viral pathogens is clinically desirable. We evaluated two multiplex reverse transcription-PCR (RT-PCR) products coupled with microarray-based systems ...
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pub_type: 杂志文章
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更新日期:2007-07-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.5.965-969.1990
更新日期:1990-05-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) encodes 11 envelope glycoproteins, of which glycoprotein G-1 (gG-1) induces a type-specific antibody response. Variability of the gG-1 gene among wild-type strains may be a factor of importance for a reliable serodiagnosis and typing of HSV-1 isolates. Here, we used a gG-1 type-spec...
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pub_type: 杂志文章
doi:10.1128/jcm.40.9.3245-3251.2002
更新日期:2002-09-01 00:00:00
abstract::MARC-145 cell line is commonly used to isolate porcine reproductive and respiratory syndrome virus (PRRSV) for diagnostics, research and vaccine production, but it yields frustratingly low success rates of virus isolation (VI). ZMAC cell line, derived from porcine alveolar macrophages, has become available but its uti...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2020-12-02 00:00:00