Performance of commercial reverse line blot assays for human papillomavirus genotyping.

abstract：:Specific precipitating and opsonic neutralizing antigens were identified in dilute-nitrous acid extracts of group A streptococci. This was done by decreasing the temperature and time of exposure of the streptococcal cells to the nitrous acid. Ninety percent of 31 strains of group A streptococcal dilute-nitrous acid ex...

journal_title：Journal of clinical microbiology

authors： Hafez K,El Kholy AM,Facklam RR

更新日期：1981-11-01 00:00:00

abstract：:The region surrounding mecA in methicillin-resistant Staphylococcus aureus (MRSA) is highly variable. We describe an approach for the rapid genotyping of MRSA by assaying for the presence or absence of variable or mobile elements previously shown to be associated with the mecA region. ...

journal_title：Journal of clinical microbiology

authors： Huygens F,Nimmo GR,Schooneveldt J,Munckhof WJ,Giffard PM

更新日期：2002-08-01 00:00:00

abstract：:DNA probes (Gen-Probe, San Diego, Calif.) directed at the Mycobacterium tuberculosis complex and Mycobacterium avium-M. intracellulare complex were used to identify acid-fast bacilli directly from specimens grown in BACTEC 12B bottles (Becton Dickinson and Co., Towson, Md.). Clinical specimens were inoculated directly...

journal_title：Journal of clinical microbiology

authors： Peterson EM,Lu R,Floyd C,Nakasone A,Friedly G,de la Maza LM

更新日期：1989-07-01 00:00:00

abstract：:The DNA hybridization assay for genes encoding for Escherichia coli enterotoxins was used to examine water specimens in Thailand. In a reconstruction experiment, the DNA hybridization assay was 10(4) times more sensitive than testing random E. coli in the Y-1 adrenal and suckling mouse assays in identifying enterotoxi...

journal_title：Journal of clinical microbiology

authors： Echeverria P,Seriwatana J,Chityothin O,Chaicumpa W,Tirapat C

更新日期：1982-12-01 00:00:00

abstract：:While high-copy-number IS6110-based restriction fragment length polymorphism (HCN-RFLP) is the gold standard for typing most Mycobacterium tuberculosis strains, the time taken for culturing and low throughput make it impractical for large-scale prospective typing of large numbers of isolates. The development of a new ...

journal_title：Journal of clinical microbiology

authors： Gopaul KK,Brown TJ,Gibson AL,Yates MD,Drobniewski FA

更新日期：2006-07-01 00:00:00

abstract：:Misidentification of coagulase-negative staphylococci (CoNS) may delay appropriate treatment. We investigated 20 clinical isolates identified as Kocuria spp. by the Vitek 2 system. All were identified as CoNS by 16S rRNA gene sequencing (18 Staphylococcus epidermidis, 1 Staphylococcus haemolyticus). Four Kocuria isola...

journal_title：Journal of clinical microbiology

authors： Ben-Ami R,Navon-Venezia S,Schwartz D,Schlezinger Y,Mekuzas Y,Carmeli Y

更新日期：2005-03-01 00:00:00

abstract：:Salmonella enterica serotype Typhi clinical isolates (n = 91) resistant to nalidixic acid (Nal(r)) were collected from sporadic cases and minor outbreaks throughout Vietnam between 1996 and 2004. These isolates were typed and compared by four methods: Vi phage typing, PstI ribotyping, XbaI and SpeI pulsed-field gel el...

journal_title：Journal of clinical microbiology

authors： Le TA,Fabre L,Roumagnac P,Grimont PA,Scavizzi MR,Weill FX

更新日期：2007-11-01 00:00:00

abstract：:A microagglutination test for Francisella tularensis, with 0.025-ml amounts of diluted sera and 0.025-ml amounts of safranin-O-stained antigen in U-bottom microtitration plates, was compared with a tube agglutination test by using 137 sera. There was 86.3% agreement (+/- 1 dilution variation) between the microagglutin...

journal_title：Journal of clinical microbiology

authors： Brown SL,McKinney FT,Klein GC,Jones WL

更新日期：1980-02-01 00:00:00

abstract：:Hepatitis B surface antigen was concentrated and purified from plasma by two simple steps of purification. In the first step the antigen was purified 24-fold by polyethylene glycol precipitation. An additional 10-fold purification was achieved by batchwise adsorption to hydroxylapatite and subsequent elution with 0.02...

journal_title：Journal of clinical microbiology

authors： Vnek J,Prince AM

更新日期：1976-06-01 00:00:00

abstract：:The emergence of a clinically daptomycin-resistant Staphylococcus aureus isolate occurred during treatment of methicillin-resistant S. aureus bacteremia and probable vertebral osteomyelitis. The breakthrough isolate was indistinguishable from pretreatment daptomycin-susceptible isolates by pulsed-field gel electrophor...

journal_title：Journal of clinical microbiology

authors： Marty FM,Yeh WW,Wennersten CB,Venkataraman L,Albano E,Alyea EP,Gold HS,Baden LR,Pillai SK

更新日期：2006-02-01 00:00:00

abstract：:Sputum obtained from patients with cystic fibrosis (CF) is highly viscous and often heterogeneous in bacterial distribution. Adding dithiothreitol (DTT) is the standard method for liquefaction prior to processing sputum for molecular detection assays. To determine if DTT treatment homogenizes the bacterial distributio...

journal_title：Journal of clinical microbiology

authors： Stokell JR,Khan A,Steck TR

更新日期：2014-07-01 00:00:00

abstract：:The Oxi-ferm tube, an eight-compartment system suitable for the recognition of nonfermentative, gram-negative, rod-shaped bacteria, was evaluated in conjunction with the oxidase test in a clinical microbiology laboratory setting. A comparison between this system and the 17-test routine examination was performed with 2...

journal_title：Journal of clinical microbiology

authors： Isenberg HD,Sampson-Scherer J

更新日期：1977-03-01 00:00:00

abstract：:A duplex PCR to detect Bordetella pertussis and Bordetella parapertussis was developed with the insertion sequences IS481 (B. pertussis) and IS1001 (B. parapertussis) and evaluated with specimens from 520 consecutive patients presenting with possible pertussis. No culture-positive-PCR-negative results occurred, giving...

journal_title：Journal of clinical microbiology

authors： Farrell DJ,Daggard G,Mukkur TK

更新日期：1999-03-01 00:00:00

abstract：:We compared the results of Gram staining and culture of cerebrospinal fluid to results obtained with a rapid PCR assay for the diagnosis of meningococcal meningitis in 281 cases of suspected bacterial meningitis. PCR had a sensitivity of 97% compared to a sensitivity of 55% for culture, and the PCR specificity was 99....

journal_title：Journal of clinical microbiology

authors： Richardson DC,Louie L,Louie M,Simor AE

更新日期：2003-08-01 00:00:00

abstract：:The taxonomic status and structure of Streptococcus dysgalactiae have been the object of much confusion. Bacteria belonging to this species are usually referred to as Lancefield group C or group G streptococci in clinical settings in spite of the fact that these terms lack precision and prevent recognition of the exac...

journal_title：Journal of clinical microbiology

authors： Jensen A,Kilian M

更新日期：2012-01-01 00:00:00

abstract：:Nucleic acid amplification tests (NAATs) are recommended for the diagnosis of N. gonorrhoeae infections because of their superior sensitivity. Increasing NAAT use causes a decline in crucial antimicrobial resistance (AMR) surveillance data, which rely on culture. We analyzed the suitability of the ESwab system for NAA...

journal_title：Journal of clinical microbiology

authors： Wind CM,de Vries HJ,Schim van der Loeff MF,Unemo M,van Dam AP

更新日期：2015-06-01 00:00:00

abstract：:The identity of each of 239 yeasts, encompassing 9 genera and 30 species, was determined with the Quantum II and API 20C identification systems. With API 20C results accepted as being correct, Quantum II proved to be 92% accurate in identification of common isolates, e.g., Candida albicans and Torulopsis glabrata, but...

journal_title：Journal of clinical microbiology

authors： Salkin IF,Schadow KH,Bankaitis LA,McGinnis MR,Kemna ME

更新日期：1985-09-01 00:00:00

abstract：:The causes of sarcoidosis are unknown. In this study, we report the presence of Mycobacterium tuberculosis complex and Propionibacterium granulosum DNA in a significant proportion of Greek patients with sarcoidosis. Human herpesvirus 8 DNA was not detected in sarcoid tissues from Greek patients. Our findings are discu...

journal_title：Journal of clinical microbiology

authors： Gazouli M,Ikonomopoulos J,Trigidou R,Foteinou M,Kittas C,Gorgoulis V

更新日期：2002-08-01 00:00:00

abstract：:A serotyping scheme for Vibrio vulnificus predicated on the detection of lipopolysaccharide (LPS) antigens is proposed. The serovar O typing scheme used to type V. vulnificus employs polyclonal antisera raised in rabbits immunized with heat-killed whole-cell vaccines. Polyclonal typing sera produced in this manner cro...

journal_title：Journal of clinical microbiology

authors： Martin SJ,Siebeling RJ

更新日期：1991-08-01 00:00:00

abstract：:Six isolates of Mycobacterium avium of genotype dnaJ(+) IS901(-) IS1311(+) IS1245(+) and serotypes 6 (n = 1), 6/9, (n = 2), and 9 (n = 3) were obtained within a 5-month period from a human immunodeficiency virus-negative patient treated for tuberculosis. The isolates were identified with PvuII restriction fragment len...

journal_title：Journal of clinical microbiology

authors： Dvorska L,Bartos M,Ostadal O,Kaustova J,Matlova L,Pavlik I

更新日期：2002-10-01 00:00:00

abstract：:Determination of the G and P serotypes of group A bovine rotaviruses from 149 samples of feces or intestinal contents collected from calves showing clinical signs of neonatal diarrhea was performed by a nested reverse transcription-PCR typing assay. The G6 serotype was the most prevalent, accounting for viruses in 55....

journal_title：Journal of clinical microbiology

authors： Falcone E,Tarantino M,Di Trani L,Cordioli P,Lavazza A,Tollis M

更新日期：1999-12-01 00:00:00

abstract：:A competitive inhibition enzyme-linked immunosorbent assay (ELISA) based on a major surface protein 5 (MSP5) B-cell epitope conserved among Anaplasma species was used to detect goats infected with Anaplasma ovis. We examined strains of A. ovis isolated from goats in Kenya and demonstrated that MSP5 and the target B-ce...

journal_title：Journal of clinical microbiology

authors： Ndung'u LW,Aguirre C,Rurangirwa FR,McElwain TF,McGuire TC,Knowles DP,Palmer GH

更新日期：1995-03-01 00:00:00

abstract：:The first outbreak of Candida haemulonii fungemia is described. The seven isolates from the blood of four neonates were identified by DNA sequencing of the ribosomal DNA. They were all resistant to amphotericin B, fluconazole, and itraconazole. This report highlights the emergence of C. haemulonii as an opportunistic ...

journal_title：Journal of clinical microbiology

authors： Khan ZU,Al-Sweih NA,Ahmad S,Al-Kazemi N,Khan S,Joseph L,Chandy R

更新日期：2007-06-01 00:00:00

abstract：:Since the introduction of antiviral compounds such as lamivudine and famciclovir in the treatment schedules of patients with chronic hepatitis B virus (HBV) infection, the accumulation of a variety of mutations in the HBV polymerase gene has been observed. The selection of these mutations is generally considered the c...

journal_title：Journal of clinical microbiology

authors： Stuyver L,Van Geyt C,De Gendt S,Van Reybroeck G,Zoulim F,Leroux-Roels G,Rossau R

更新日期：2000-02-01 00:00:00

abstract：:In a collaborative study at three university hospitals, the recovery of microorganisms and the speed of detection of microbial growth by the BacT/Alert (Organon Teknika Corporation, Durham, N.C.) and BACTEC 660/730 (Becton-Dickinson Diagnostic Instrument Systems, Sparks, Md.) nonradiometric blood culture systems were ...

journal_title：Journal of clinical microbiology

authors： Wilson ML,Weinstein MP,Reimer LG,Mirrett S,Reller LB

更新日期：1992-02-01 00:00:00

abstract：:The purpose of this study was to determine whether individual milk samples can replace serum samples for the detection of bovine herpesvirus 1 (BHV1) glycoprotein E (gE)-specific antibodies. Serum and milk samples were collected at the same time from cattle in BHV1-free herds, cattle in unvaccinated herds, and cattle ...

journal_title：Journal of clinical microbiology

authors： Wellenberg GJ,Verstraten ER,Mars MH,Van Oirschot JT

更新日期：1998-02-01 00:00:00

abstract：:A urease-based enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of reagin antibodies in serum. Visuwell Reagin (ADI Diagnostics Inc., Rexdale, Ontario, Canada) is a non-treponemal screening test for the serodiagnosis of syphilis which has the benefits of large batch testing, automatabilit...

journal_title：Journal of clinical microbiology

authors： White TJ,Fuller SA

更新日期：1989-10-01 00:00:00

abstract：:Acinetobacter isolates associated with casualties from the Iraq conflict from the United States were compared with those from the United Kingdom by pulsed-field gel electrophoresis and integron analysis. Representatives of the main outbreak strain associated with casualties from both countries were indistinguishable i...

journal_title：Journal of clinical microbiology

authors： Turton JF,Kaufmann ME,Gill MJ,Pike R,Scott PT,Fishbain J,Craft D,Deye G,Riddell S,Lindler LE,Pitt TL

更新日期：2006-07-01 00:00:00

abstract：:In 32 patients for whom highly active antiretroviral therapy was failing, a good agreement between drug resistance-associated mutations in plasma and peripheral blood mononuclear cells (PBMCs) was found (k = 0.85). The mutations with the lowest agreement were 20R, 63P, and 84V in the protease gene and 184V in the reve...

journal_title：Journal of clinical microbiology

authors： Sarmati L,Nicastri E,Uccella I,D'Ettorre G,Parisi SG,Palmisano L,Galluzzo C,Concia E,Vullo V,Vella S,Andreoni M

更新日期：2003-04-01 00:00:00

abstract：:Of 4,000 endocervical specimens tested with the Chlamydiazyme enzyme-linked immunosorbent assay (Abbott Laboratories), 233 (5.8%) gave positive results (A492 above the cutoff), which were confirmed with a blocking reagent (Abbott). An additional 34 specimens (14.6%) with chlamydial antigen were detected and confirmed ...

journal_title：Journal of clinical microbiology

authors： Kellogg JA,Seiple JW,Stroll ES

更新日期：1993-06-01 00:00:00