Capillary gas chromatographic analysis of carbohydrates of Legionella pneumophila and other members of the family Legionellaceae.


:Legionella pneumophila, the causative agent of Legionnaires disease, and related organisms have previously been characterized primarily by conventional bacteriological methods, DNA-DNA hybridization, antigenic analysis, and fatty acid analysis. By capillary gas chromatographic analysis for carbohydrates, we have shown that muramic acid and glucosamine, characteristic markers of bacterial cell walls, were present in samples of L. pneumophila and a group of legionella-like organisms. Some bacterial samples contained two unusual isomeric aminodideoxyhexoses (X1 and X2). L. pneumophila was characterized by the absence of fucose and the presence of the peak X1. Tatlockia micdadei (Legionella micdadei) was distinguishable by the presence of large amounts of rhamnose and fucose and by the absence of X1 and X2. Fluoribacter strains were much more variable in their carbohydrate composition. These data suggest that, in addition to other reported techniques, carbohydrate profiling by capillary gas chromatography can be a valuable diagnostic method in reference microbiology laboratories for differentiating members of the family Legionellaceae.


J Clin Microbiol


Fox A,Lau PY,Brown A,Morgan SL,Zhu ZT,Lema M,Walla MD




Has Abstract


1984-03-01 00:00:00












  • Actinomyces naeslundii as an agent of pelvic actinomycosis in the presence of an intrauterine device.

    abstract::Actinomyces naeslundii is a saprophyte, sometimes a pathogen, of the human oral cavity. Very few extra-oral infections related to this agent have been described. We report the first instance of A. naeslundii as an etiological agent of pelvic actinomycosis in a user of an intrauterine device, an infection so far exclus...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Bonnez W,Lattimer G,Mohanraj NA,Johnson TH

    更新日期:1985-02-01 00:00:00

  • Complete genome analysis of coxsackievirus A2, A4, A5, and A10 strains isolated from hand, foot, and mouth disease patients in China revealing frequent recombination of human enterovirus A.

    abstract::Coxsackievirus (CV) strains CVA2, CVA4, CVA5, and CVA10 were isolated from patients with hand, foot, and mouth disease during a 2009 outbreak in China. Full genome sequences for four representative strains, CVA2/SD/CHN/09 (A2SD09), CVA4/SZ/CHN/09 (A4SZ09), CVA5/SD/CHN/09 (A5SD09), and CVA10/SD/CHN/09 (A10SD09), were d...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Hu YF,Yang F,Du J,Dong J,Zhang T,Wu ZQ,Xue Y,Jin Q

    更新日期:2011-07-01 00:00:00

  • Evaluation of the NG-Test MCR-1 Lateral Flow Assay and EDTA-Colistin Broth Disk Elution Methods To Detect Plasmid-Mediated Colistin Resistance among Gram-Negative Bacterial Isolates.

    abstract::Plasmid-mediated colistin resistance (PMCR) is a global public health concern, given its ease of transmissibility. The purpose of this study was to evaluate two methods for the detection of PMCR from bacterial colonies: (i) the NG-Test MCR-1 lateral flow immunoassay (LFA; NG Biotech, Guipry, France) and (ii) the EDTA-...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Fenwick AJ,Bergman Y,Lewis S,Yee R,Uhlemann AC,Cole N,Kohner P,Ordak C,Green DA,Schuetz AN,Humphries R,Simner PJ

    更新日期:2020-03-25 00:00:00

  • Genotypic and phenotypic characterization of methicillin-susceptible Staphylococcus aureus isolates misidentified as methicillin-resistant Staphylococcus aureus by the BD GeneOhm MRSA assay.

    abstract::Twenty-three nasal swab samples that tested positive for methicillin-resistant Staphylococcus aureus (MRSA) on initial testing by the BD GeneOhm MRSA assay (BD-MRSA PCR; BD GeneOhm, San Diego, CA) were culture positive only for methicillin-susceptible S. aureus (MSSA) from an enrichment broth. The 23 recovered isolate...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Stamper PD,Louie L,Wong H,Simor AE,Farley JE,Carroll KC

    更新日期:2011-04-01 00:00:00

  • Detection of Blastomyces dermatitidis and Histoplasma capsulatum from culture isolates and clinical specimens by use of real-time PCR.

    abstract::Blastomyces dermatitidis and Histoplasma capsulatum are dimorphic fungi that often cause self-limited respiratory infections. However, they may also cause severe disseminated disease, depending on the level of the exposure to the organism and the host immune status. In addition, patients with infections caused by thes...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Babady NE,Buckwalter SP,Hall L,Le Febre KM,Binnicker MJ,Wengenack NL

    更新日期:2011-09-01 00:00:00

  • Modified coagglutination procedure for the serological grouping of streptococci.

    abstract::Cowan I staphylococci coated with antisera to streptococcal groups A, B, C, D, F, and G were used as coagglutination reagents in a modified coagglutination procedure (MCAP). Streptococcal group antigens were extracted with a Streptomyces albus-lysozyme enzyme mixture for 30 min at 55 degrees C and centrifuged, and the...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Carlson JR,McCarthy LR

    更新日期:1979-03-01 00:00:00

  • Isothermal microcalorimetry to study drugs against Schistosoma mansoni.

    abstract::Alternative antischistosomal drugs are required since praziquantel is virtually the only drug available for treatment and morbidity control of schistosomiasis. Manual microscopic reading is the current "gold standard" to assess the in vitro antischistosomal properties of test drugs; however, it is labor-intensive, sub...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Manneck T,Braissant O,Haggenmüller Y,Keiser J

    更新日期:2011-04-01 00:00:00

  • Differentiation between wild and vaccine-derived strains of poliovirus by stringent microplate hybridization of PCR products.

    abstract::Procedures for differentiation between wild and vaccine-derived strains of poliovirus are required, particularly in countries where wild and vaccine-related strains coexist. For this differentiation, we tested the method of Inouye and Hondo (S. Inouye and R. Hondo, Arch. Virol. 129:311-316, 1993) for discrimination of...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Takeda N,Sakae K,Agboatwalla M,Isomura S,Hondo R,Inouye S

    更新日期:1994-01-01 00:00:00

  • Identification of optimal epitopes for Plasmodium falciparum rapid diagnostic tests that target histidine-rich proteins 2 and 3.

    abstract::Rapid diagnostic tests (RDTs) represent important tools to diagnose malaria infection. To improve understanding of the variable performance of RDTs that detect the major target in Plasmodium falciparum, namely, histidine-rich protein 2 (HRP2), and to inform the design of better tests, we undertook detailed mapping of ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lee N,Gatton ML,Pelecanos A,Bubb M,Gonzalez I,Bell D,Cheng Q,McCarthy JS

    更新日期:2012-04-01 00:00:00

  • Diagnosis of placental malaria.

    abstract::In a group of 596 delivering Ghanaian women, the sensitivities of peripheral blood thick film microscopy, ICT Malaria P.f/P.v test, and PCR in detecting microscopically confirmed placental Plasmodium falciparum infection were 42, 80, and 97%, respectively. In addition to the gross underestimation of placental malaria ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Mockenhaupt FP,Ulmen U,von Gaertner C,Bedu-Addo G,Bienzle U

    更新日期:2002-01-01 00:00:00

  • Rapid detection of Legionella species in bronchoalveolar lavage fluids with the EnviroAmp Legionella PCR amplification and detection kit.

    abstract::A molecular assay based on a rapid DNA extraction protocol and the EnviroAmp Legionella Kits was used to detect Legionella species in bronchoalveolar fluid specimens. All Legionella strains isolated from tap water in hospitals could be detected distinctly. Both sensitivity and specificity were tested. In a prospective...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kessler HH,Reinthaler FF,Pschaid A,Pierer K,Kleinhappl B,Eber E,Marth E

    更新日期:1993-12-01 00:00:00

  • Multilocus sequence typing of Pneumocystis jirovecii from clinical samples: how many and which loci should be used?

    abstract::Pneumocystis jirovecii pneumonia (PCP) is an opportunistic infection with airborne transmission and remains a major cause of respiratory illness among immunocompromised individuals. In recent years, several outbreaks of PCP, occurring mostly in kidney transplant recipients, have been reported. Currently, multilocus se...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Maitte C,Leterrier M,Le Pape P,Miegeville M,Morio F

    更新日期:2013-09-01 00:00:00

  • Taxonomic subgroups of Pasteurella multocida correlate with clinical presentation.

    abstract::Pasteurella multocida is a small nonmotile gram-negative coccobacillus that is found in the nasopharynx and gastrointestinal tract of many wild and domesticated animals. In humans it most commonly causes cellulitis and localized superficial skin abscesses following an animal bite or scratch. The respiratory tract is t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Chen HI,Hulten K,Clarridge JE 3rd

    更新日期:2002-09-01 00:00:00

  • Distinguishing Candida species by beta-N-acetylhexosaminidase activity.

    abstract::A variety of fungi produce the hydrolytic enzyme beta-N-acetylhexosaminidase (HexNAcase), which can be readily detected in assays by using p-nitrophenyl-N-acetyl-beta-D-glucosaminide as a substrate. In the present study we developed a microtiter plate-based HexNAcase assay for distinguishing Candida albicans and Candi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Niimi K,Shepherd MG,Cannon RD

    更新日期:2001-06-01 00:00:00

  • Rapid and sensitive assay for detection of enterotoxigenic Bacteroides fragilis.

    abstract::Bacteroides fragilis is an obligatory anaerobic, gram-negative bacterium found among the normal intestinal flora of humans. Enterotoxigenic strains of B. fragilis (ETBF) have been associated with diarrheal diseases in humans and animals. The enterotoxin of ETBF induces fluid changes in ligated intestinal segments and ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Zhang G,Weintraub A

    更新日期:1998-12-01 00:00:00

  • Rapid detection of methicillin-resistant Staphylococcus aureus strains not identified by slide agglutination tests.

    abstract::Seventy-nine methicillin-resistant Staphylococcus aureus (MRSA) strains, isolated during 1980 to 1990, were classified as MRSA Aggl- (14 strains) and MRSA Aggl+ (65 strains) strains on the basis of test results in slide agglutination assays designed to detect fibrinogen-binding protein (clumping factor) and protein A ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kuusela P,Hildén P,Savolainen K,Vuento M,Lyytikäinen O,Vuopio-Varkila J

    更新日期:1994-01-01 00:00:00

  • Human Fc(gamma) receptors for differentiation in throat cultures of group C "Streptococcus equisimilis" and group C "Streptococcus milleri".

    abstract::The biochemical characteristics and the presence of human Fc(gamma) receptors of 52 throat isolates of group C beta-hemolytic streptococci were examined. Among these isolates, 38 were identified as "Streptococcus milleri" and 14 were identified as "Streptococcus equisimilis." The differentiation of group C "S. equisim...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lebrun L,Guibert M,Wallet P,de Maneville MM,Pillot J

    更新日期:1986-11-01 00:00:00

  • Genome sequencing of Mycobacterium abscessus isolates from patients in the united states and comparisons to globally diverse clinical strains.

    abstract::Nontuberculous mycobacterial infections caused by Mycobacterium abscessus are responsible for a range of disease manifestations from pulmonary to skin infections and are notoriously difficult to treat, due to innate resistance to many antibiotics. Previous population studies of clinical M. abscessus isolates utilized ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Davidson RM,Hasan NA,Reynolds PR,Totten S,Garcia B,Levin A,Ramamoorthy P,Heifets L,Daley CL,Strong M

    更新日期:2014-10-01 00:00:00

  • Use of the restriction enzyme EcoRI for pulsed-field gel electrophoretic analysis of Helicobacter pylori.

    abstract::Pulsed-field gel electrophoretic (PFGE) analysis of Helicobacter pylori isolates is not commonly employed because of the inability to compare the typing with other typing systems. We adapted the PFGE analysis for H. pylori by using EcoRI and slightly modified our laboratory methods to improve the typing of isolates (t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Hosaka Y,Irinoda K,Nakano R,Tanabe S,Koizumi W,Saigenji K,Inoue M

    更新日期:2005-02-01 00:00:00

  • Comparison of phenotypic and genotypic methods for detection of diphtheria toxin among isolates of pathogenic corynebacteria.

    abstract::We have compared molecular, immunochemical, and cytotoxic assays for the detection of diphtheria toxin from 55 isolates of Corynebacterium diphtheriae and Corynebacterium ulcerans originally isolated in five different countries. The suitabilities and accuracies of these assays for the laboratory diagnosis of diphtheri...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Efstratiou A,Engler KH,Dawes CS,Sesardic D

    更新日期:1998-11-01 00:00:00

  • Short-Read Whole-Genome Sequencing for Laboratory-Based Surveillance of Bordetella pertussis.

    abstract::Bordetella pertussis is a Gram-negative bacterium that causes respiratory infections in humans. Ongoing molecular surveillance of B. pertussis acellular vaccine (aP) antigens is critical for understanding the interaction between evolutionary pressures, disease pathogenesis, and vaccine effectiveness. Methods currently...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Marchand-Austin A,Tsang RSW,Guthrie JL,Ma JH,Lim GH,Crowcroft NS,Deeks SL,Farrell DJ,Jamieson FB

    更新日期:2017-05-01 00:00:00

  • Bovine tuberculosis (Mycobacterium bovis) in wildlife in Spain.

    abstract::Mycobacterium bovis infection in wildlife and feral species is a potential source of infection for livestock and a threat to protected and endangered species. The aim of this study was to identify Spanish wild animal species infected with M. bovis through bacteriological culture and spacer oligonucleotide typing (spol...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Aranaz A,De Juan L,Montero N,Sánchez C,Galka M,Delso C,Alvarez J,Romero B,Bezos J,Vela AI,Briones V,Mateos A,Domínguez L

    更新日期:2004-06-01 00:00:00

  • Comparison of four plating media for isolating Vibrio cholerae.

    abstract::Two brands of thiosulfate citrate bile salts sucrose agar and Monsur taurocholate tellurite gelatin (TTG) agar were compared with two newly developed media, sucrose tellurite teepol agar and Vibrio parahaemolyticus agar for isolation and identification of Vibrio cholerae. The thiosulfate citrate bile salts sucrose and...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Morris GK,Merson MH,Huq I,Kibrya AK,Black R

    更新日期:1979-01-01 00:00:00

  • Identification of Mycobacterium kansasii by using a DNA probe (AccuProbe) and molecular techniques.

    abstract::The newly formulated Mycobacterium kansasii AccuProbe was evaluated, and the results obtained with the new version were compared to the results obtained with the old version of this test by using 116 M. kansasii strains, 1 Mycobacterium gastri strain, and 19 strains of several mycobacterial species. The sensitivity of...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Richter E,Niemann S,Rüsch-Gerdes S,Hoffner S

    更新日期:1999-04-01 00:00:00

  • Relationship between photochromogenicity and test temperature in mycobacteria.

    abstract::Although it has been recommended that photochromogenicity of mycobacteria should be tested after incubation at 25 to 30 degrees C for 2 weeks (Wayne et al., Int. J. Syst. Bacteriol. 24:412-419, 1974), our results indicate that photochromogenicity should be determined at 37 degrees C in the dark and under continuous ex...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Tsukamura M

    更新日期:1981-08-01 00:00:00

  • Seroepidemiological study of African horse sickness virus in The Gambia.

    abstract::An indirect enzyme-linked immunosorbent assay was used for the screening of horse sera from The Gambia for antibodies against African horse sickness virus (AHSV). The AHSV antigen used for coating was semipurified according to the method of Manning and Chen (Curr. Microbiol. 4:381, 1980); control mock-infected Vero ce...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Staeuber N,Fye B,Zinsstag J,McCullough KC

    更新日期:1993-08-01 00:00:00

  • Age dependence of adenovirus-specific neutralizing antibody titers in individuals from sub-Saharan Africa.

    abstract::We assessed neutralizing antibody titers to adenovirus serotype 5 (Ad5) and six rare adenovirus serotypes, serotypes 11, 35, 50, 26, 48, and 49, in pediatric populations in sub-Saharan Africa. We observed a clear age dependence of Ad5-specific neutralizing antibody titers. These data will help to guide the development...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Thorner AR,Vogels R,Kaspers J,Weverling GJ,Holterman L,Lemckert AA,Dilraj A,McNally LM,Jeena PM,Jepsen S,Abbink P,Nanda A,Swanson PE,Bates AT,O'Brien KL,Havenga MJ,Goudsmit J,Barouch DH

    更新日期:2006-10-01 00:00:00

  • Molecular typing of Penicillium marneffei isolates from Thailand by NotI macrorestriction and pulsed-field gel electrophoresis.

    abstract::Penicillium marneffei is recognized as one of the most frequently detected opportunistic pathogens of AIDS patients in northern Thailand. We undertook a genomic epidemiology study of 64 P. marneffei isolates collected from immunosuppressed patients by pulsed-field gel electrophoresis (PFGE) with restriction enzyme Not...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Trewatcharegon S,Sirisinha S,Romsai A,Eampokalap B,Teanpaisan R,Chaiyaroj SC

    更新日期:2001-12-01 00:00:00

  • Incidental finding of Myxobolus spores (protozoa: myxozoa) in stool samples from patients with gastrointestinal symptoms.

    abstract::Myxozoan spores were detected in fecal samples from three patients presenting with abdominal pain and/or diarrhea. The spores were identical to those of Myxobolus plectroplites, a previously described pathogen from the freshwater fish Plectroplites ambiguus. All patients had recently eaten fish caught from local water...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Boreham RE,Hendrick S,O'Donoghue PJ,Stenzel DJ

    更新日期:1998-12-01 00:00:00

  • Prospective comparison of whole-blood- and plasma-based hepatitis C virus RNA detection systems: improved detection using whole blood as the source of viral RNA.

    abstract::We previously demonstrated that whole blood contains significantly more hepatitis C virus (HCV) RNA than plasma. To validate the whole-blood-based HCV RNA detection method, a prospective comparison of HCV RNA detection in whole blood and plasma from 50 patients with chronic liver disease was undertaken. Whole-blood an...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Stapleton JT,Klinzman D,Schmidt WN,Pfaller MA,Wu P,LaBrecque DR,Han Jq,Phillips MJ,Woolson R,Alden B

    更新日期:1999-03-01 00:00:00