Molecular characterization of pneumococcal nasopharynx isolates collected from children during their first 2 years of life.

abstract：:Recent studies have speculated on the possible role of the mother in transmitting Helicobacter pylori infection to their children. In an attempt to either prove or disprove this supposition, we investigated the rates of infection of children born to H. pylori-positive mothers from birth to 5 years of age using serolog...

journal_title：Journal of clinical microbiology

authors： Konno M,Fujii N,Yokota S,Sato K,Takahashi M,Sato K,Mino E,Sugiyama T

更新日期：2005-05-01 00:00:00

abstract：:Detection of Staphylococcus aureus isolates with intermediate vancomycin susceptibility (VISA) and heteroresistance (hVISA) remains problematic. The population analysis profile/area under the curve (PAP/AUC) is the gold standard but is cumbersome. We compared the performance of two Etest screening methods (macromethod...

journal_title：Journal of clinical microbiology

authors： Riederer K,Shemes S,Chase P,Musta A,Mar A,Khatib R

更新日期：2011-06-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of measles immunoglobulin G antibody (MEASELISA). This assay was found to be comparable to the measles hemagglutination inhibition (HAI) test. Approximately 500 sera from three centers were tested by MEASELISA and the HAI test. MEASELISA demo...

journal_title：Journal of clinical microbiology

authors： Boteler WL,Luipersbeck PM,Fuccillo DA,O'Beirne AJ

更新日期：1983-05-01 00:00:00

abstract：:Cytomegalovirus (CMV) DNA loads in paired leukocyte and plasma samples from 199 patient visits by 66 patients with CMV retinitis were determined. Leukocyte CMV load determinations had a greater range of values (mean, 24,587 copies/10(6) leukocytes; maximum, 539, 000) than did plasma CMV load determinations (mean, 10,3...

journal_title：Journal of clinical microbiology

authors： Jabs DA,Forman M,Enger C,Jackson JB

更新日期：1999-05-01 00:00:00

abstract：:Nineteen reference and 156 clinical strains of the genus Nocardia belonging to 12 taxonomic groups were studied for restriction fragment length polymorphism (RFLP) by using an amplified 439-bp segment of the 65-kDa heat shock protein gene. Of 30 restriction endonucleases, digestion with MspI and then digestion with Bs...

journal_title：Journal of clinical microbiology

authors： Steingrube VA,Brown BA,Gibson JL,Wilson RW,Brown J,Blacklock Z,Jost K,Locke S,Ulrich RF,Wallace RJ Jr

更新日期：1995-12-01 00:00:00

abstract：:During an 8.5-year period (1974 to 1982), 56 Yersinia enterocolitica isolates from patients residing mainly in the New York City area were studied. Evaluation of these isolates revealed a marked increase in bacteremic episodes caused by strains of serogroups of O:3 and O:5,27 and the overall emergence of serogroup O:3...

journal_title：Journal of clinical microbiology

authors： Bottone EJ

更新日期：1983-01-01 00:00:00

abstract：:Human adenoviruses (AdVs) typically cause mild illnesses in otherwise healthy hosts. We investigated a pediatric outbreak of acute respiratory infection with fatal outcomes that occurred in Lisbon, Portugal, in 2004. Biological specimens were collected from 83 children attending two nurseries, a kinesiotherapy clinic,...

journal_title：Journal of clinical microbiology

authors： Rebelo-de-Andrade H,Pereira C,Gíria M,Prudêncio E,Brito MJ,Calé E,Taveira N

更新日期：2010-04-01 00:00:00

abstract：:Streptococcus pneumoniae is both a commensal and a major pathogen that causes invasive disease in people of all ages. The introduction of serotype-specific pneumococcal vaccines has reduced the burden of disease but has also led to replacement with new strains; thus, serotyping remains important for vaccine-related di...

journal_title：Journal of clinical microbiology

authors： Pholwat S,Sakai F,Turner P,Vidal JE,Houpt ER

更新日期：2016-07-01 00:00:00

abstract：:The HERPCHEK herpes simplex virus (HSV) antigen test (Du Pont, Billerica, Mass.) is a biotinstreptavidin-amplified enzyme-linked immunoassay for the detection of HSV antigen directly in clinical specimens. In a total of 200 mucocutaneous specimens, predominantly lesions from nongenital sites, HERPCHEK had a sensitivit...

journal_title：Journal of clinical microbiology

authors： Wu TC,Zaza S,Callaway J

更新日期：1989-08-01 00:00:00

abstract：:A multiplex method using the SNaPshot technique was developed to screen for six common mycobacterial species: Mycobacteria tuberculosis, M. avium, M. intracellulare, M. chelonae, M. kansasii, and M. gordonae. A total of 468 mycobacterial clinical isolates were subjected to analysis for the presence of the six mycobact...

journal_title：Journal of clinical microbiology

authors： Wang H,Yue J,Han M,Yang J,Zhao Y

更新日期：2010-01-01 00:00:00

abstract：:Cryptococcus neoformans var. gattii is a causative agent of cryptococcosis and is thought to have a specific ecological association with a number of Eucalyptus species in Australia. However, the role that the tree plays in the life cycle of the fungus and the nature of the infectious propagule are not well understood....

journal_title：Journal of clinical microbiology

authors： Halliday CL,Carter DA

更新日期：2003-02-01 00:00:00

abstract：:A recent case report of a child infected with enterohemorrhagic Escherichia coli (EHEC) of serotype O118:H16 in Bavaria, in association with the isolation of a bovine O118 strain on the same farm (A. Weber, H. Klie, H. Richter, P. Gallien, M. Timm, and K. W. Perlberg, Berl. Muench. Tieraerztl. Wochenschr. 110:211-213,...

journal_title：Journal of clinical microbiology

authors： Wieler LH,Busse B,Steinrück H,Beutin L,Weber A,Karch H,Baljer G

更新日期：2000-06-01 00:00:00

abstract：:A microagglutination test for Francisella tularensis, with 0.025-ml amounts of diluted sera and 0.025-ml amounts of safranin-O-stained antigen in U-bottom microtitration plates, was compared with a tube agglutination test by using 137 sera. There was 86.3% agreement (+/- 1 dilution variation) between the microagglutin...

journal_title：Journal of clinical microbiology

authors： Brown SL,McKinney FT,Klein GC,Jones WL

更新日期：1980-02-01 00:00:00

abstract：:Human papillomavirus type 16 (HPV 16) plays a cardinal role in the pathogenesis of cervical cancer. HPV 16 has intratypic variants which show different geographical distributions and different oncogenic potentials. To analyze the presence of sequence variations of HPV 16 variants in northeast China, 71 cervical carcin...

journal_title：Journal of clinical microbiology

authors： Shang Q,Wang Y,Fang Y,Wei L,Chen S,Sun Y,Li B,Zhang F,Gu H

更新日期：2011-07-01 00:00:00

abstract：:Legionella pneumophila, the causative agent of Legionnaires disease, and related organisms have previously been characterized primarily by conventional bacteriological methods, DNA-DNA hybridization, antigenic analysis, and fatty acid analysis. By capillary gas chromatographic analysis for carbohydrates, we have shown...

journal_title：Journal of clinical microbiology

authors： Fox A,Lau PY,Brown A,Morgan SL,Zhu ZT,Lema M,Walla MD

更新日期：1984-03-01 00:00:00

abstract：:Transfusion-acquired cytomegalovirus (CMV) infections should be prevented in seronegative immunocompromised patients by providing blood products from donors who are also seronegative. Latex agglutination was investigated as a simple and rapid method for detecting antibody against CMV. Latex beads were coated with CMV ...

journal_title：Journal of clinical microbiology

authors： Adler SP,McVoy M,Biro VG,Britt WJ,Hider P,Marshall D

更新日期：1985-07-01 00:00:00

abstract：:Detection of residual plasma viremia in antiretroviral therapy (ART)-suppressed HIV-infected individuals is critical for characterizing the latent reservoir and evaluating the impact of cure interventions. Ultracentrifugation-based single-copy assays are sensitive but labor intensive. Fully automated replicate testing...

journal_title：Journal of clinical microbiology

authors： Bakkour S,Deng X,Bacchetti P,Grebe E,Montalvo L,Worlock A,Stone M,Deeks SG,Richman DD,Busch MP

更新日期：2020-11-18 00:00:00

abstract：:Automated DNA sequencing was used to characterize mutations associated with rifampin resistance in a 69-bp region of the gene, rpoB, encoding the beta subunit of RNA polymerase in Mycobacterium tuberculosis. The data confirmed that greater than 90% of rifampin-resistant strains have sequence alterations in this region...

journal_title：Journal of clinical microbiology

authors： Kapur V,Li LL,Iordanescu S,Hamrick MR,Wanger A,Kreiswirth BN,Musser JM

更新日期：1994-04-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay was developed to study the subclass distribution of immunoglobulin G (IgG) specific to the core glycolipid (CGL) of the Re mutant of Salmonella minnesota R595 in serum samples from individuals with an IgG response toward these antigens. In a group of healthy blood donors, we detect...

journal_title：Journal of clinical microbiology

authors： Nys M,Joassin L,Somzee A,Demonty J

更新日期：1988-05-01 00:00:00

abstract：:Serological markers for hepatitis B virus in male homosexuals demonstrated a high prevalence of past and present infection. Seropositivity of 91% for hepatitis B e antigen or antibody was demonstrated by radioimmunoassay in hepatitis B surface antigen-positive specimens. ...

journal_title：Journal of clinical microbiology

authors： Murphy BL,Schreeder MT,Maynard JE,Hadler SC,Sheller MJ

更新日期：1980-03-01 00:00:00

abstract：:Human (n = 33) and canine (n = 53) Campylobacter upsaliensis isolates from seven countries were genotyped by a new amplified fragment length polymorphism method. We observed 100% typeability and high overall diversity. The majority of human strains (23/33) clustered separately from canine strains, indicating that dogs...

journal_title：Journal of clinical microbiology

authors： Damborg P,Guardabassi L,Pedersen K,Kokotovic B

更新日期：2008-04-01 00:00:00

abstract：:Murine monoclonal antibodies (MAbs) were produced which were specific for Pasteurella haemolytica serotype 1 lipopolysaccharide (LPS). The MAbs also reacted with LPS present in a partially purified antigen derived from a saline extract of the organism. The epitope to which the MAbs were directed was a carbohydrate whi...

journal_title：Journal of clinical microbiology

authors： Durham JA,Antone SM,Cunningham MW,Confer AW

更新日期：1988-05-01 00:00:00

abstract：:Neutralizing serum antibody to serotype 9 rotavirus WI61 was detected in 41% of 870 Ecuadorian children and 26% of 140 German children. In both areas an age-related prevalence increase was observed. We identified 11 serum samples from Ecuadorian children which neutralized exclusively serotype 9 rotavirus. Thirteen of ...

journal_title：Journal of clinical microbiology

authors： Brüssow H,Clark HF,Sidoti J

更新日期：1991-01-01 00:00:00

abstract：:Pluronic polyols markedly improved the growth of two human lymphocyte cell lines when added to the growth medium in concentrations of 0.05 to 0.1%. The results of the current studies suggest that, in addition to the protective effect of polyols against mechanical damage of mammalian cells in submerged cultures, the pl...

journal_title：Journal of clinical microbiology

authors： Mizrahi A

更新日期：1975-07-01 00:00:00

abstract：:The subgroups and serotypes of 178 strains of rotavirus isolated from diarrheic and healthy children in Bangui, Central African Republic, during a 27-month period were determined by enzyme-linked immunosorbent assay. The subgroup was determined for 152 of the viral strains, 18.4% being subgroup I and 81.6% being subgr...

journal_title：Journal of clinical microbiology

authors： Georges-Courbot MC,Beraud AM,Beards GM,Campbell AD,Gonzalez JP,Georges AJ,Flewett TH

更新日期：1988-04-01 00:00:00

abstract：:A total of 1,298 nonfermentative gram-negative rods were used to evaluate the performance of the individual biochemical tests in the N/F System and to determine whether the observed results included sufficient key reactions for rapid identification. The system included an oxidase test, two screening tubes providing 5 ...

journal_title：Journal of clinical microbiology

authors： Barnishan J,Ayers LW

更新日期：1979-02-01 00:00:00

abstract：:Quantitative lactate determinations were performed on cerebrospinal fluids to assess their value in the rapid diagnosis of bacterial and mycotic meningitis and to evaluate their value in assessing the prognosis in these patients. Cerebrospinal fluid lactate concentrations were elevated in all patients with untreated b...

journal_title：Journal of clinical microbiology

authors： Komorowski RA,Farmer SG,Hanson GA,Hause LL

更新日期：1978-07-01 00:00:00

abstract：:A study of single stool specimens was done to determine the prevalence of intestinal parasites among 1,000 primary school children. A questionnaire was completed by each child's parents. Specimens were examined by using wet-mount preparation, formaline-ether concentration, and Sheather's flotation technique. Trichrome...

journal_title：Journal of clinical microbiology

authors： Nimri L,Batchoun R

更新日期：1994-11-01 00:00:00

abstract：:A total of 189 Candida albicans isolates have been typed by multilocus enzyme electrophoresis. The results obtained confirm the clonal mode of reproduction of C. albicans. The C. albicans populations found in the oropharynx of human immunodeficiency virus (HIV)-infected patients, in the oropharynx of healthy carriers,...

journal_title：Journal of clinical microbiology

authors： Boerlin P,Boerlin-Petzold F,Goudet J,Durussel C,Pagani JL,Chave JP,Bille J

更新日期：1996-05-01 00:00:00

abstract：:The Echinococcus Western Blot IgG (LDBIO Diagnostics, Lyon, France), using a whole larval antigen from Echinococcus multilocularis, was evaluated for serodiagnosis and differentiation between two human parasitic infections of worldwide importance: cystic echinococcosis, due to Echinococcus granulosus, and alveolar ech...

journal_title：Journal of clinical microbiology

authors： Liance M,Janin V,Bresson-Hadni S,Vuitton DA,Houin R,Piarroux R

更新日期：2000-10-01 00:00:00