Genetic diversity of PspA types among nasopharyngeal isolates collected during an ongoing surveillance study of children in Brazil.

abstract：:The PLEX-ID system is a novel technology that couples PCR amplification and electrospray ionization-mass spectrometry to identify pathogens directly in clinical specimens. The analytical performance of the PLEX-ID Broad Fungal assay was compared with that of traditional culture identification by using 91 characterized...

journal_title：Journal of clinical microbiology

authors： Simner PJ,Uhl JR,Hall L,Weber MM,Walchak RC,Buckwalter S,Wengenack NL

更新日期：2013-06-01 00:00:00

abstract：:Blood culture bottles with antimicrobial removal systems are recommended for patients who develop fever while on antibiotics. This study compared the ability of Becton Dickinson (Sparks, MD) BACTEC PLUS bottles and bioMerieux (Durham, NC) BacT/Alert FA bottles to effectively remove vancomycin, cefoxitin, ceftriaxone, ...

journal_title：Journal of clinical microbiology

authors： Flayhart D,Borek AP,Wakefield T,Dick J,Carroll KC

更新日期：2007-03-01 00:00:00

abstract：:The causative agent of Johne's disease is Mycobacterium avium subsp. paratuberculosis. This is a chronic, debilitating gastrointestinal disorder that affects ruminants and is responsible for significant economic loss. The specimen processing method that combines C(18)-carboxypropylbetaine (CB-18) treatment and lytic e...

journal_title：Journal of clinical microbiology

authors： Thornton CG,MacLellan KM,Stabel JR,Carothers C,Whitlock RH,Passen S

更新日期：2002-05-01 00:00:00

abstract：:Cag pathogenicity island-containing Helicobacter pylori (type I) induces signal transduction pathways resulting in tyrosine phosphorylation of proteins adjacent to the site of bacterial adhesion on host gastric epithelial cells. Conventional block PCR-restriction fragment length polymorphism (RFLP) and real-time Light...

journal_title：Journal of clinical microbiology

authors： Owen RJ,Sharp SI,Chisholm SA,Rijpkema S

更新日期：2003-07-01 00:00:00

abstract：:A bacitracin (0.04 units, BBL)-SXT (trimethoprim, 1.25 mg, plus sulfamethoxazole, 23.75 mg, BBL) susceptibility test was 94% accurate in presumptively identifying streptococci as either group A, B or not group A and B. ...

journal_title：Journal of clinical microbiology

authors： Gunn BA

更新日期：1976-08-01 00:00:00

abstract：:Metarhizium viride has been associated with fatal systemic mycoses in chameleons, but subsequent data on mycoses caused by this fungus in reptiles are lacking. The aim of this investigation was therefore to obtain information on the presence of M. viride in reptiles kept as pets in captivity and its association with c...

journal_title：Journal of clinical microbiology

authors： Schmidt V,Klasen L,Schneider J,Hübel J,Pees M

更新日期：2017-03-01 00:00:00

abstract：:Buruli ulcer (BU) is a necrotizing infection of skin and soft tissue caused by Mycobacterium ulcerans. In Australia, most cases of BU are linked to temperate, coastal Victoria and tropical, northern Queensland, and strains from these regions are distinguishable by variable-number tandem repeat (VNTR) typing. We presen...

journal_title：Journal of clinical microbiology

authors： Lavender CJ,Globan M,Johnson PD,Charles PG,Jenkin GA,Ghosh N,Clark BM,Martinello M,Fyfe JA

更新日期：2012-11-01 00:00:00

abstract：:A study of immunofluorescence of yeast in the urine was carried out in 18 patients with funguria in an effort to correlate gamma globulin coating with evidence of tissue invasion or as an indicator for institution of therapy. Positive immunofluorescence of yeast in urine is common but is neither indicative of upper ur...

journal_title：Journal of clinical microbiology

authors： Everett ED,Eickhoff TC,Ehret JM

更新日期：1976-08-01 00:00:00

abstract：:The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) has infected at least 1,082 people, including 439 fatalities. So far, no empirical virus isolation study has been done to elucidate infectious virus secretion or serotype variability. Here, we used 51 respiratory samples from 32 patients with co...

journal_title：Journal of clinical microbiology

authors： Muth D,Corman VM,Meyer B,Assiri A,Al-Masri M,Farah M,Steinhagen K,Lattwein E,Al-Tawfiq JA,Albarrak A,Müller MA,Drosten C,Memish ZA

更新日期：2015-09-01 00:00:00

abstract：:We evaluated the clinical usefulness of species identification of blood isolates of coagulase-negative staphylococci as a predictor of the clinical significance of the isolates. In addition, we compared results of species identification obtained with MicroScan Rapid Gram-Positive Identification panels and Dried Overni...

journal_title：Journal of clinical microbiology

authors： Weinstein MP,Mirrett S,Van Pelt L,McKinnon M,Zimmer BL,Kloos W,Reller LB

更新日期：1998-07-01 00:00:00

abstract：:Rapid detection of mycobacterial disease is essential. Using multiple specimen types and concentrations of mycobacteria, we compared two commercial auramine O stains. The more rapid stain permitted consistent acid-fast bacillus quantitation and exhibited less debris staining, and the staining procedure required less t...

journal_title：Journal of clinical microbiology

authors： Hendry C,Dionne K,Hedgepeth A,Carroll K,Parrish N

更新日期：2009-04-01 00:00:00

abstract：:The increased incidence of nosocomial Legionnaires' disease in two hospitals prompted investigation of possible environmental sources. In the search for an effective DNA-typing technique for use in hospital epidemiology, the performance and convenience of three methods-SfiI macrorestriction analysis (MRA), amplified f...

journal_title：Journal of clinical microbiology

authors： Jonas D,Meyer HG,Matthes P,Hartung D,Jahn B,Daschner FD,Jansen B

更新日期：2000-06-01 00:00:00

abstract：:Nasopharyngeal secretions from adults and children were obtained in Stockholm, Sweden, for routine diagnosis of influenza A virus, influenza B virus, respiratory syncytial (RS) virus, parainfluenza type 3 virus, and adenovirus infections by demonstration of viral antigens directly in the specimens. The cells in nasoph...

journal_title：Journal of clinical microbiology

authors： Grandien M,Pettersson CA,Gardner PS,Linde A,Stanton A

更新日期：1985-11-01 00:00:00

abstract：:Rapid tests for glucose, maltose, lactose, sucrose, and starch fermentation were performed on 112 strains of anaerobic bacteria. The tests were incubated under aerobic conditions, and results were read within 4 h. An overall correlation of 89% was achieved between the rapid tests and the Virginia Polytechnic Institut...

journal_title：Journal of clinical microbiology

authors： Schreckenberger PC,Blazevic DJ

更新日期：1976-03-01 00:00:00

abstract：:The ability of Staphylococcus aureus to clot plasma through conformational activation of prothrombin by staphylocoagulase is used to distinguish S. aureus from coagulase-negative staphylococci. We show that while the direct thrombin inhibitor dabigatran inhibits staphylocoagulase activity, the clinical use of dabigatr...

journal_title：Journal of clinical microbiology

authors： Vanassche T,Verhaegen J,Peetermans WE,Hoylaerts MF,Verhamme P

更新日期：2010-11-01 00:00:00

abstract：:In this study, the currently known typing methods for Mycobacterium tuberculosis isolates were evaluated with regard to reproducibility, discrimination, and specificity. Therefore, 90 M. tuberculosis complex strains, originating from 38 countries, were tested in five restriction fragment length polymorphism (RFLP) typ...

journal_title：Journal of clinical microbiology

authors： Kremer K,van Soolingen D,Frothingham R,Haas WH,Hermans PW,Martín C,Palittapongarnpim P,Plikaytis BB,Riley LW,Yakrus MA,Musser JM,van Embden JD

更新日期：1999-08-01 00:00:00

abstract：:A total of 101 food-borne and waterborne outbreaks that were caused by norovirus and that resulted in more than 4,100 cases of illness were reported to the Swedish Institute for Infectious Disease Control from January 2002 to December 2006. Sequence and epidemiological data for isolates from 73 outbreaks were analyzed...

journal_title：Journal of clinical microbiology

authors： Lysén M,Thorhagen M,Brytting M,Hjertqvist M,Andersson Y,Hedlund KO

更新日期：2009-08-01 00:00:00

abstract：:Imferon, an iron-dextran complex, enhances the growth of Neisseria gonorrhoeae and N. meningitidis. The use of Imferon as a replacement for ferric nitrate, in a defined supplement for GC agar significantly increased the average colony sizes of both gonococci and meningococci. In comparison with Thayer-Martin medium, I...

journal_title：Journal of clinical microbiology

authors： Payne SM,Finkelstein RA

更新日期：1977-09-01 00:00:00

abstract：:This study evaluated the performance of Directigen FluA combined with a 3-day flu screening culture for the detection of influenza virus. This abbreviated protocol was a useful and effective tool and resulted in a substantial reduction in time, effort, and money spent, while not compromising sensitivity of influenza v...

journal_title：Journal of clinical microbiology

authors： Newton DW,Mellen CF,Baxter BD,Atmar RL,Menegus MA

更新日期：2002-11-01 00:00:00

abstract：:A cluster of methicillin-resistant Staphylococcus aureus (MRSA) infections among patients on an intensive care unit (ICU) was detected by routine infection control surveillance. In the period from 5 January to 22 June 1995, 10 patients on the ICU and a further 6 patients (5 on one ward that had received colonized pati...

journal_title：Journal of clinical microbiology

authors： Tambic A,Power EG,Talsania H,Anthony RM,French GL

更新日期：1997-12-01 00:00:00

abstract：:Several biochemical test systems were studied for their potential usefulness for the examination of strains of Campylobacter species. Most (81%) of the C. jejuni strains hydrolyzed sodium hippurate, but strains of C. fetus, C. sputorum, and C. fecalis did not. Some (46%) of the C. jejuni strains and all of the C. sput...

journal_title：Journal of clinical microbiology

authors： Hébert GA,Hollis DG,Weaver RE,Lambert MA,Blaser MJ,Moss CW

更新日期：1982-06-01 00:00:00

abstract：:In this study we evaluated the ability of three commercial methods, API 20A (Analytab Products, Plainview, N.Y.), Minitek (BBL Microbiology Systems, Cockeysville, Md.), and Anaerobe-Tek (Flow Laboratories, Inc., McLean, Va.), to accurately identify 165 recent clinical and 38 stock isolates of anaerobic bacteria withou...

journal_title：Journal of clinical microbiology

authors： Appelbaum PC,Kaufmann CS,Keifer JC,Venbrux HJ

更新日期：1983-09-01 00:00:00

abstract：:Panton-Valentine leukocidin (PVL) is a virulence factor of Staphylococcus aureus, which is associated with skin and soft-tissue infections and necrotizing pneumonia. To develop a rapid phenotypic assay, recombinant PVL F component was used to generate monoclonal antibodies by phage display. These antibodies were spott...

journal_title：Journal of clinical microbiology

authors： Monecke S,Müller E,Buechler J,Rejman J,Stieber B,Akpaka PE,Bandt D,Burris R,Coombs G,Hidalgo-Arroyo GA,Hughes P,Kearns A,Abós SM,Pichon B,Skakni L,Söderquist B,Ehricht R

更新日期：2013-02-01 00:00:00

abstract：:Nonfermenting bacteria are ubiquitous environmental opportunists that cause infections in humans, especially compromised patients. Due to their limited biochemical reactivity and different morphotypes, misidentification by classical phenotypic means occurs frequently. Therefore, we evaluated the use of matrix-assisted...

journal_title：Journal of clinical microbiology

authors： Mellmann A,Cloud J,Maier T,Keckevoet U,Ramminger I,Iwen P,Dunn J,Hall G,Wilson D,Lasala P,Kostrzewa M,Harmsen D

更新日期：2008-06-01 00:00:00

abstract：:By using the computer-assisted Dendron system to analyze the patterns of Southern blots probed with the repetitive sequence Ca3, we have compared oral isolates of Candida spp. from a group of 11 nonhospitalized patients with AIDS suffering from recurrent episodes of oral thrush in Leicester, England, with oral isolate...

journal_title：Journal of clinical microbiology

authors： Schmid J,Odds FC,Wiselka MJ,Nicholson KG,Soll DR

更新日期：1992-04-01 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) has occasionally been associated with cases of interstitial pneumonitis, mainly in individuals with impaired cellular immunity. Here we report for the first time severe interstitial pneumonitis with simultaneous HHV-6 and Pneumocystis carinii infections in the lung tissue of a young patient...

journal_title：Journal of clinical microbiology

authors： Vuorinen T,Kotilainen P,Lautenschlager I,Kujari H,Krogerus L,Oksi J

更新日期：2004-11-01 00:00:00

abstract：:We compared the Mycoplasma Duo kit (Sanofi Diagnostics Pasteur) with PCR for detection of Ureaplasma spp. in endotracheal aspirates from 60 premature neonates. The overall agreement between the two tests was 96%. The Mycoplasma Duo assay is a useful alternative to culture and PCR for detection of neonatal Ureaplasma i...

journal_title：Journal of clinical microbiology

authors： Cheah FC,Anderson TP,Darlow BA,Murdoch DR

更新日期：2005-01-01 00:00:00

abstract：:This study evaluates the performance of a PCR assay for the detection of Pneumocystis carinii from respiratory specimens that has been designed for use in the clinical microbiology laboratory. The test includes a simple method for nucleic acid extraction and amplification, a colorimetric probe hybridization technique ...

journal_title：Journal of clinical microbiology

authors： Caliendo AM,Hewitt PL,Allega JM,Keen A,Ruoff KL,Ferraro MJ

更新日期：1998-04-01 00:00:00

abstract：:A rapid dimethyl sulfoxide modification of an acid-fast technique was applied to direct fecal smears to monitor cryptosporidiosis in nonhuman primates. Brilliantly stained pink oocysts against a pale green background demonstrated well-preserved internal morphology and facilitated rapid, simple, noninvasive diagnosis w...

journal_title：Journal of clinical microbiology

authors： Bronsdon MA

更新日期：1984-06-01 00:00:00

abstract：:A rapid and simple method for extracting specific cell-free antigens of Coccidioides immitis and Histoplasma capsulatum from agar slant cultures was developed. The extracts were analyzed in immunodiffusion tests for the presence of C. immitis or H. capsulatum specific antigens. These extracts were compared with cultur...

journal_title：Journal of clinical microbiology

authors： Kaufman L,Standard P

更新日期：1978-07-01 00:00:00