Clinical importance of identifying coagulase-negative staphylococci isolated from blood cultures: evaluation of MicroScan Rapid and Dried Overnight Gram-Positive panels versus a conventional reference method.


:We evaluated the clinical usefulness of species identification of blood isolates of coagulase-negative staphylococci as a predictor of the clinical significance of the isolates. In addition, we compared results of species identification obtained with MicroScan Rapid Gram-Positive Identification panels and Dried Overnight (Conventional) Gram-Positive Identification panels with those obtained by a tube reference method. Two hundred eighty-five blood isolates were tested, including 92 judged to represent true bacteremia and 193 judged to represent contamination. The most common species detected were Staphylococcus epidermidis, Staphylococcus hominis, and Staphylococcus haemolyticus. These three species accounted for nearly 98% of the clinically significant isolates and 89% of the contaminants. The isolation of other species almost always represented contamination. However, identification of the three most common species did not help distinguish pathogens from contaminants. Both the Rapid and the Dried Overnight Gram-Positive panels identified S. epidermidis strains accurately, but the panels performed less well for the other species. Analysis revealed that S. hominis was frequently misidentified due to the presence of a previously unknown subspecies. Based on the initial results, revised investigational Dried Overnight Gram-Positive Identification panels (CPID-2) were prepared and tested. The CPID-2 panels identified 85 to 95% of S. epidermidis strains, 76 to 86% of S. hominis strains, and 88 to 92% of S. haemolyticus strains with high probability (>85%) and, overall, represented a significant improvement over the other panels for identification of these staphylococcal species.


J Clin Microbiol


Weinstein MP,Mirrett S,Van Pelt L,McKinnon M,Zimmer BL,Kloos W,Reller LB




Has Abstract


1998-07-01 00:00:00












  • Diagnosis of bacteremia in whole-blood samples by use of a commercial universal 16S rRNA gene-based PCR and sequence analysis.

    abstract::In a prospective, multicenter study of 342 blood samples from 187 patients with systemic inflammatory response syndrome, sepsis, or neutropenic fever, a new commercial PCR test (SepsiTest; Molzym) was evaluated for rapid diagnosis of bacteremia. The test comprises a universal PCR from the 16S rRNA gene, with subsequen...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究


    authors: Wellinghausen N,Kochem AJ,Disqué C,Mühl H,Gebert S,Winter J,Matten J,Sakka SG

    更新日期:2009-09-01 00:00:00

  • Reverse transcription-PCR detection of hepatitis G virus.

    abstract::Hepatitis G virus (HGV) was recently identified as a new member of the Flaviviridae, but its clinical significance is still unclear. Since no immunoassay for the diagnosis of HGV is available, we developed a sensitive reverse transcription-PCR (RT-PCR) assay to facilitate the detection of the viral genome by mass scre...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Schlueter V,Schmolke S,Stark K,Hess G,Ofenloch-Haehnle B,Engel AM

    更新日期:1996-11-01 00:00:00

  • Geographical heterogeneity between Far Eastern and Western countries in prevalence of the virulence plasmid, the superantigen Yersinia pseudotuberculosis-derived mitogen, and the high-pathogenicity island among Yersinia pseudotuberculosis strains.

    abstract::Yersinia pseudotuberculosis produces novel superantigenic toxins designated YPMa (Y. pseudotuberculosis-derived mitogen), YPMb, and YPMc and has a pathogenicity island termed HPI (high-pathogenicity island) and R-HPI (the right-hand part of the HPI with truncation in its left-hand part) on the chromosome. Analysis of ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Fukushima H,Matsuda Y,Seki R,Tsubokura M,Takeda N,Shubin FN,Paik IK,Zheng XB

    更新日期:2001-10-01 00:00:00

  • Method for testing for human papillomavirus infection in patients with cervical intraepithelial disease.

    abstract::To determine whether DNA detection of high-risk human papillomavirus could represent a reliable screening technique and a useful follow-up method, we investigated the performance of the Hybrid Capture II assay in detecting high-grade cervical lesions. The test positivity at 1.86 pg/ml had a high sensitivity (94.7%) an...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lin HP,Huang YY,Wu HY,Kao JT

    更新日期:2004-01-01 00:00:00

  • A three-year study of Campylobacter jejuni genotypes in humans with domestically acquired infections and in chicken samples from the Helsinki area.

    abstract::Campylobacter jejuni isolates from stool samples of patients with domestically acquired sporadic infections and from chicken from retail shops were studied during seasonal peaks from June to September over a 3-year period from 1996 to 1998. A large number of pulsed-field gel electrophoresis (PFGE) genotypes (a combine...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Hänninen ML,Perko-Mäkelä P,Pitkälä A,Rautelin H

    更新日期:2000-05-01 00:00:00

  • Characterization of clinically significant strains of coagulase-negative staphylococci.

    abstract::On occasion, a patient may have two or more clinical cultures yielding a coagulase-negative staphylococcus If these multiple isolates have the same phenotype, one might conclude that the same strain was reisolated from the patient, indicating its persistent and pathological presence. We examined the validity of this c...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Christensen GD,Parisi JT,Bisno AL,Simpson WA,Beachey EH

    更新日期:1983-08-01 00:00:00

  • Molecular follow-up of Salmonella enterica subsp. enterica serovar Agona infection in cattle and humans.

    abstract::Salmonella enterica subsp. enterica serovar Agona was not frequently encountered in Finland until an increase in rates of isolation among animal and feed was seen in 1994. A small outbreak among cattle farms in the regions of Oulu and Vaasa in northwestern Finland in 1994-1995 included eight farms. After the outbreak,...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lindqvist N,Siitonen A,Pelkonen S

    更新日期:2002-10-01 00:00:00

  • Multiple-locus variable-number tandem-repeat analysis of 201 Mycoplasma pneumoniae isolates from Beijing, China, from 2008 to 2011.

    abstract::A total of 201 Mycoplasma pneumoniae clinical isolates from Beijing, China, isolated from 2008 to 2011, were clustered into 16 multiple-locus variable-number tandem-repeat analysis (MLVA) types, of which 6 new MLVA types have never been reported previously. Type 1 isolates based on p1 gene genotyping were mainly MLVA ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Zhao F,Liu G,Cao B,Wu J,Gu Y,He L,Meng F,Zhu L,Yin Y,Lv M,Zhang J

    更新日期:2013-02-01 00:00:00

  • Identification of Mycobacterium kansasii by using a DNA probe (AccuProbe) and molecular techniques.

    abstract::The newly formulated Mycobacterium kansasii AccuProbe was evaluated, and the results obtained with the new version were compared to the results obtained with the old version of this test by using 116 M. kansasii strains, 1 Mycobacterium gastri strain, and 19 strains of several mycobacterial species. The sensitivity of...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Richter E,Niemann S,Rüsch-Gerdes S,Hoffner S

    更新日期:1999-04-01 00:00:00

  • Characterization of Leuconostoc lactis strains from human sources.

    abstract::An examination of 23 vancomycin-resistant, streptococcuslike isolates of clinical origin revealed a group of 14 to be related to Leuconostoc lactis (type strain, CIP 102422) on the basis of chemotaxonomic studies. These isolates were initially shown to be atypical by classical biochemical tests. However, they were cha...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Barreau C,Wagener G

    更新日期:1990-08-01 00:00:00

  • Detection of pathogenic protozoa in the diagnostic laboratory: result reproducibility, specimen pooling, and competency assessment.

    abstract::Stool microscopy as performed in clinical parasitology laboratories is a complex procedure with subjective interpretation. Quality assurance (QA) programs often emphasize proficiency testing as an assessment tool. We describe a result reproducibility assessment tool, which can form part of a broader QA program, and wh...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Libman MD,Gyorkos TW,Kokoskin E,Maclean JD

    更新日期:2008-07-01 00:00:00

  • Comparison of the BD MAX enteric bacterial panel to routine culture methods for detection of Campylobacter, enterohemorrhagic Escherichia coli (O157), Salmonella, and Shigella isolates in preserved stool specimens.

    abstract::We compared the BD MAX enteric bacterial panel (EBP) to culture for the detection of Salmonella, Shigella, Campylobacter, and Shiga toxin-producing enterohemorrhagic Escherichia coli (EHEC) O157 in seeded stool samples. The EBP panel demonstrated superior sensitivity and reliably detected Salmonella, EHEC O157, Shigel...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Anderson NW,Buchan BW,Ledeboer NA

    更新日期:2014-04-01 00:00:00

  • Hemo-De as substitute for ethyl acetate in formalin-ethyl acetate concentration technique.

    abstract::In comparative studies, Hemo-De (PMP Medical Industries, Inc., Irving, Tex.) was found to be a suitable replacement for ethyl acetate in the Formalin-ethyl acetate concentration technique. With essentially equivalent recovery rates for both procedures, the Formalin-Hemo-De concentration technique is considered to be t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Neimeister R,Logan AL,Gerber B,Egleton JH,Kleger B

    更新日期:1987-02-01 00:00:00

  • Genetic background and antibiotic resistance of Staphylococcus aureus strains isolated in the Republic of Georgia.

    abstract::The genetic composition and antibiotic sensitivities of 50 clinical isolates of Staphylococcus aureus obtained from various clinics in the Republic of Georgia were characterized. S. aureus strains ATCC 700699 and ATCC 29737 were included as reference standards in all analyses. All 52 strains had identical 16S rRNA pro...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Revazishvili T,Bakanidze L,Gomelauri T,Zhgenti E,Chanturia G,Kekelidze M,Rajanna C,Kreger A,Sulakvelidze A

    更新日期:2006-10-01 00:00:00

  • Extended-spectrum-beta-lactamase production in a Salmonella enterica serotype Typhi strain from the Philippines.

    abstract::A Salmonella enterica serotype Typhi strain was cultured from blood and fecal samples from a 54-year-old man with fever and diarrhea. He had returned from travel to the Philippines a few days earlier. Phenotypic and genotypic analysis confirmed the production of the SHV-12 extended-spectrum beta-lactamase. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Al Naiemi N,Zwart B,Rijnsburger MC,Roosendaal R,Debets-Ossenkopp YJ,Mulder JA,Fijen CA,Maten W,Vandenbroucke-Grauls CM,Savelkoul PH

    更新日期:2008-08-01 00:00:00

  • Large-scale comparative genomics meta-analysis of Campylobacter jejuni isolates reveals low level of genome plasticity.

    abstract::We have used comparative genomic hybridization (CGH) on a full-genome Campylobacter jejuni microarray to examine genome-wide gene conservation patterns among 51 strains isolated from food and clinical sources. These data have been integrated with data from three previous C. jejuni CGH studies to perform a meta-analysi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,meta分析


    authors: Taboada EN,Acedillo RR,Carrillo CD,Findlay WA,Medeiros DT,Mykytczuk OL,Roberts MJ,Valencia CA,Farber JM,Nash JH

    更新日期:2004-10-01 00:00:00

  • Methods for identification of Staphylococcus aureus isolates in cases of bovine mastitis.

    abstract::A total of 272 staphylococcal isolates from cases of bovine mastitis (159 Staphylococcus aureus) belonging to 12 different species were identified with ID32 STAPH galleries, and 51 of them were confirmed by 16S rRNA gene (rrs) sequencing. The same isolates were examined for their hemolytic activity on sheep blood agar...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Boerlin P,Kuhnert P,Hüssy D,Schaellibaum M

    更新日期:2003-02-01 00:00:00

  • Identification of human H1N2 and human-swine reassortant H1N2 and H1N1 influenza A viruses among pigs in Ontario, Canada (2003 to 2005).

    abstract::Since 2003, three novel genotypes of H1 influenza viruses have been recovered from Canadian pigs, including a wholly human H1N2 virus and human-swine reassortants. These isolates demonstrate that human-lineage H1N2 viruses are infectious for pigs and that viruses with a human PB1/swine PA/swine PB2 polymerase complex ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Karasin AI,Carman S,Olsen CW

    更新日期:2006-03-01 00:00:00

  • Seroepidemiological studies of Borrelia burgdorferi infection in sheep in Norway.

    abstract::An enzyme-linked immunosorbent assay for antibodies to Borrelia burgdorferi in sheep was established. The assay was used in a seroepidemiological survey in which serum samples from 327 Norwegian rams were screened for antibodies to B. burgdorferi. These rams were randomly chosen, clinically healthy animals from all pa...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Fridriksdóttir V,Nesse LL,Gudding R

    更新日期:1992-05-01 00:00:00

  • Use of immunoelectron microscopy to demonstrate Francisella tularensis.

    abstract::Three immunoelectron microscopy (IEM) methods were employed to show laboratory-cultivated Francisella tularensis. By the IEM assays, F. tularensis was distinguished from four antigenically distinct gram-negative bacteria. IEM should be a valuable tool for confirming presumptive isolates of F. tularensis and may potent...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Geisbert TW,Jahrling PB,Ezzell JW Jr

    更新日期:1993-07-01 00:00:00

  • Whole-Genome Multilocus Sequence Typing of Extended-Spectrum-Beta-Lactamase-Producing Enterobacteriaceae.

    abstract::Molecular typing has become indispensable in the detection of nosocomial transmission of bacterial pathogens and the identification of sources and routes of transmission in outbreak settings, but current methods are labor-intensive, are difficult to standardize, or have limited resolution. Whole-genome multilocus sequ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kluytmans-van den Bergh MF,Rossen JW,Bruijning-Verhagen PC,Bonten MJ,Friedrich AW,Vandenbroucke-Grauls CM,Willems RJ,Kluytmans JA

    更新日期:2016-12-01 00:00:00

  • Multicenter Evaluation of BD Max Enteric Parasite Real-Time PCR Assay for Detection of Giardia duodenalis, Cryptosporidium hominis, Cryptosporidium parvum, and Entamoeba histolytica.

    abstract::Common causes of chronic diarrhea among travelers worldwide include protozoan parasites. The majority of parasitic infections are caused by Giardia duodenalis, Entamoeba histolytica, Cryptosporidium parvum, and Cryptosporidium hominis Similarly, these species cause the majority of parasitic diarrhea acquired in the Un...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究


    authors: Madison-Antenucci S,Relich RF,Doyle L,Espina N,Fuller D,Karchmer T,Lainesse A,Mortensen JE,Pancholi P,Veros W,Harrington SM

    更新日期:2016-11-01 00:00:00

  • Rapid and specific detection of Mycobacterium tuberculosis by using the Smart Cycler instrument and a specific fluorogenic probe.

    abstract::A procedure using the Smart Cycler instrument and a fluorescence quencher (FQ) probe for the specific identification of Mycobacterium tuberculosis complex (MTB) was used to detect organisms in 366 acid-fast bacillus smear-positive respiratory specimens. It was compared to culture and the AMPLICOR M. tuberculosis PCR t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Cleary TJ,Roudel G,Casillas O,Miller N

    更新日期:2003-10-01 00:00:00

  • The Alere BinaxNOW Pneumococcal Urinary Antigen Test: Diagnostic Sensitivity for Adult Pneumococcal Pneumonia and Relationship to Specific Serotypes.

    abstract::Little is known about the sensitivity of the BinaxNOW pneumococcal urinary antigen (PUA) test for adult pneumococcal pneumonia caused by different serotypes. In this study, we aimed to analyze the trends in the sensitivity of the PUA test over a 15-year period (2001 to 2015) and to analyze its sensitivity for pneumoco...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Shoji H,Domenech A,Simonetti AF,González A,García-Somoza D,Cubero M,Martí S,Maeda M,Tubau F,Liñares J,Domínguez MA,Carratalà J,Ardanuy C

    更新日期:2018-01-24 00:00:00

  • Comparison of Counterimmunoelectrophoresis and electron microscopy for laboratory diagnosis of human reovirus-like agent-associated infantile gastroenteritis.

    abstract::Counterimmunoelectrophoresis was compared with electron microscopy for detection of human reovirus-like agent in fecal specimens. Both tests gave very similar results. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Spence L,Fauvel M,Petro R,Bloch S

    更新日期:1977-02-01 00:00:00

  • Clustering of tuberculosis cases based on variable-number tandem-repeat typing in relation to the population structure of Mycobacterium tuberculosis in the Netherlands.

    abstract::The population structure of 3,776 Mycobacterium tuberculosis isolates was determined using variable-number tandem-repeat (VNTR) typing. The degree of clonality was so high that a more relaxed definition of clustering cannot be applied. Among recent immigrants with non-Euro-American isolates, transmission is overestima...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Sloot R,Borgdorff MW,de Beer JL,van Ingen J,Supply P,van Soolingen D

    更新日期:2013-07-01 00:00:00

  • Detection of colonization factor antigen I-positive enterotoxigenic Escherichia coli with a cloned polynucleotide probe.

    abstract::We compared a new colony hybridization assay with an established enzyme-linked immunosorbent assay for detection of enterotoxigenic Escherichia coli (ETEC) expressing colonization factor antigen I (CFA/I). The tests were applied to 135 human ETEC strains. Of these isolates, 30 had previously been characterized for CFA...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Grewal HM,Sommerfelt H,Gaastra W,Svennerholm AM,Bhan MK,Hamers AM,Kumar R,Wiklund G,Bjorvatn B

    更新日期:1990-10-01 00:00:00

  • Differentiation of Rhizomucor species by carbon source utilization and isoenzyme analysis.

    abstract::Nineteen Rhizomucor miehei and Rhizomucor pusillus isolates were assayed for their ability to utilize 87 various substrates as a single carbon source. Besides a difference in sucrose utilization, distinctive differences were found in the utilization of glycine, phenylalanine, and beta-alanine. Five isoenzyme systems a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Vastag M,Papp T,Kasza Z,Vágvölgyi C

    更新日期:1998-07-01 00:00:00

  • Genomic analysis of Mycobacterium bovis and other members of the Mycobacterium tuberculosis complex by isoenzyme analysis and pulsed-field gel electrophoresis.

    abstract::Initially, multilocus enzyme electrophoresis was used to examine genetic relationships among 63 isolates of Mycobacterium bovis and 13 other members of the M. tuberculosis complex. The isolates were divided into five electrophoretic types, with a mean genetic diversity of 0.1. The strains were genetically homogenous, ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Feizabadi MM,Robertson ID,Cousins DV,Hampson DJ

    更新日期:1996-05-01 00:00:00

  • Multiplex real-time PCR targeting the RNase P RNA gene for detection and identification of Candida species in blood.

    abstract::We have developed a single-tube multiplex real-time PCR method for the detection of the eight most common Candida species causing septicemia: Candida albicans, C. dubliniensis, C. famata, C. glabrata, C. guilliermondii, C. krusei, C. parapsilosis, and C. tropicalis. The method developed targets the RNase P RNA gene RP...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Innings A,Ullberg M,Johansson A,Rubin CJ,Noreus N,Isaksson M,Herrmann B

    更新日期:2007-03-01 00:00:00