解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract:BACKGROUND:In research laboratories using DNA-microarrays, usually a number of researchers perform experiments, each generating possible sources of error. There is a need for a quick and robust method to assess data quality and sources of errors in DNA-microarray experiments. To this end, a novel and cost-effective val...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-6-77
更新日期:2005-05-20 00:00:00
abstract::As gene expression profile data from DNA microarrays accumulate rapidly, there is a natural need to compare data across labs and platforms. Comparisons of microarray data can be quite challenging due to data complexity and variability. Different labs may adopt different technology platforms. One may ask about the degr...
journal_title:BMC genomics
pub_type: 杂志文章,多中心研究
doi:10.1186/1471-2164-6-71
更新日期:2005-05-11 00:00:00
abstract:BACKGROUND:Equally important and challenging as genome annotation, is the subsequent classification of predicted genes into their respective pathways. The Kyoto Encyclopedia of Genes and Genomes (KEGG) represents a database consisting of known genes and their respective biochemical functionalities. Although accessible ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-6-60
更新日期:2005-05-03 00:00:00
abstract:BACKGROUND:Secondary structure in the target is a property not usually considered in software applications for design of optimal custom oligonucleotide probes. It is frequently assumed that eliminating self-complementarity, or screening for secondary structure in the probe, is sufficient to avoid interference with hybr...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-6-31
更新日期:2005-03-08 00:00:00
abstract:BACKGROUND:Defensins are important components of innate immunity to combat bacterial and viral infections, and can even elicit antitumor responses. Clusters of defensin (DEF) genes are located in a 2 Mb range of the human chromosome 8p23.1. This DEF locus, however, represents one of the regions in the euchromatic part ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-5-92
更新日期:2004-12-10 00:00:00
abstract:BACKGROUND:Malaria, caused by the parasitic protist Plasmodium falciparum, represents a major public health problem in the developing world. The P. falciparum genome has been sequenced, which provides new opportunities for the identification of novel drug targets. Eukaryotic protein kinases (ePKs) form a large family o...
journal_title:BMC genomics
pub_type: 杂志文章,评审
doi:10.1186/1471-2164-5-79
更新日期:2004-10-12 00:00:00
abstract:BACKGROUND:Gene expression technologies have the ability to generate vast amounts of data, yet there often resides only limited resources for subsequent validation studies. This necessitates the ability to perform sorting and prioritization of the output data. Previously described methodologies have used functional pat...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-5-58
更新日期:2004-08-20 00:00:00
abstract:BACKGROUND:In mammalian cells changes in intracellular pH (pHi), which are predominantly controlled by activity of plasma membrane ion exchangers, regulate a diverse range of normal and pathological cellular processes. How changes in pHi affect distinct cellular processes has primarily been determined by evaluating pro...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-5-46
更新日期:2004-07-16 00:00:00
abstract:BACKGROUND:A major goal in the post-genomic era is to identify and characterise disease susceptibility genes and to apply this knowledge to disease prevention and treatment. Rodents and humans have remarkably similar genomes and share closely related biochemical, physiological and pathological pathways. In this work we...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-5-28
更新日期:2004-04-29 00:00:00
abstract:BACKGROUND:Obtaining reliable and reproducible two-color microarray gene expression data is critically important for understanding the biological significance of perturbations made on a cellular system. Microarray design, RNA preparation and labeling, hybridization conditions and data acquisition and analysis are varia...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-5-20
更新日期:2004-03-09 00:00:00
abstract:BACKGROUND:To generate specific transcript profiles, one must isolate homogenous cell populations using techniques that often yield small amounts of RNA, requiring researchers to employ RNA amplification methods. The data generated by using these methods must be extensively evaluated to determine any technique dependen...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-5-2
更新日期:2004-01-06 00:00:00
abstract:BACKGROUND:Tumor necrosis factor alpha (TNF) is able to induce a variety of biological responses in the nervous system including inflammation and neuroprotection. Human astrocytoma cells U373 have been widely used as a model for inflammatory cytokine actions in the nervous system. Here we used cDNA microarrays to analy...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-4-46
更新日期:2003-11-25 00:00:00
abstract:BACKGROUND:Subtractive hybridization methods are valuable tools for identifying differentially regulated genes in a given tissue avoiding redundant sequencing of clones representing the same expressed genes, maximizing detection of low abundant transcripts and thus, affecting the efficiency and cost effectiveness of sm...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-4-40
更新日期:2003-09-30 00:00:00
abstract:BACKGROUND:Reading disability (RD) is a common syndrome with a large genetic component. Chromosome 6 has been identified in several linkage studies as playing a significant role. A more recent study identified a peak of transmission disequilibrium to marker JA04 (G72384) on chromosome 6p22.3, suggesting that a gene is ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-4-25
更新日期:2003-06-30 00:00:00
abstract:BACKGROUND:Ralstonia solanacearum is an important plant pathogen. The genome of R. solananearum GMI1000 is organised into two replicons (a 3.7-Mb chromosome and a 2.1-Mb megaplasmid) and this bipartite genome structure is characteristic for most R. solanacearum strains. To determine whether the megaplasmid was acquired...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-4-10
更新日期:2003-03-17 00:00:00
abstract:BACKGROUND:The objective of this research was to investigate the reproducibility of cross-species microarray hybridisation. Comparisons between same- and cross-species hybridisations were also made. Nine hybridisations between a single pig skeletal muscle RNA sample and three human cDNA nylon microarrays were completed...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-3-27
更新日期:2002-09-27 00:00:00
abstract:BACKGROUND:Gonadotropin releasing hormone (GnRH) is responsible for stimulation of gonadotropic hormone (GtH) in the hypothalamus-pituitary-gonadal axis (HPG). The regulatory mechanisms responsible for brain specificity make the promoter attractive for in silico analysis and reporter gene studies in zebrafish (Danio re...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-3-25
更新日期:2002-08-21 00:00:00
abstract:BACKGROUND:Based on sequence similarity, the superfamily of G protein-coupled receptors (GPRs) can be subdivided into several subfamilies, the members of which often share similar ligands. The sequence data provided by the human genome project allows us to identify new GPRs by in silico homology screening, and to predi...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-3-17
更新日期:2002-07-05 00:00:00
abstract:BACKGROUND:Mucolipidosis type IV (MLIV) is an autosomal recessive lysosomal storage disorder characterized by severe neurologic and ophthalmologic abnormalities. Recently the MLIV gene, MCOLN1, has been identified as a new member of the transient receptor potential (TRP) cation channel superfamily. Here we report the c...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-3-3
更新日期:2002-01-01 00:00:00