abstract：:This article presents the current status of the development of bioeconomy in the Czech Republic. Although the country has no unified strategy on bioeconomy, there are ambitious governmental innovation strategies and focused strategies for each region. Traditionally, the country has had a strong research performance in...

journal_title：New biotechnology

authors： Hájek M,Holecová M,Smolová H,Jeřábek L,Frébort I

更新日期：2021-03-25 00:00:00

abstract：:Biotechnology methods and applications have the potential to accelerate a transition to a more circular economy. This article identifies five distinct points within a typical product lifecycle as areas where biotechnology can be impactful, starting with so-called 'beginning-of-life', with the ability to make many wide...

journal_title：New biotechnology

authors： Schilling C,Weiss S

更新日期：2021-01-25 00:00:00

abstract：:Light scattering is a challenge for imaging three-dimensional organisms. A number of new tissue clearing methodologies have been described in recent years, increasing the utilities of clearing techniques to obtain transparent samples. Here, we describe the optimization of a suitable and novel protocol for clearing Gal...

journal_title：New biotechnology

authors： Moya-Andérico L,Admella J,Torrents E

更新日期：2021-01-25 00:00:00

abstract：:Autoimmune diseases are frequently associated with autoantibodies. Recently, large sets of autoantibody-targeted antigens ("autoantigen-omes") of patient and control sera have been revealed, enabling autoantigen-omic approaches. However, statistical standards for defining such autoantigen-omes are lacking. The z-score...

journal_title：New biotechnology

authors： Moritz CP,Stoevesandt O,Tholance Y,Camdessanché JP,Antoine JC

更新日期：2021-01-25 00:00:00

abstract：:The lack of immunization in developing countries is undoubtedly the most serious consequence of the difficulty in accessing traditional vaccination systems. The World Health Organization (WHO) has aimed to find low-cost vaccines, which are accessible to the population and are easy to store and distribute without the n...

journal_title：New biotechnology

authors： Cebadera Miranda E,Castillo Ruiz-Cabello MV,Cámara Hurtado M

更新日期：2020-11-25 00:00:00

abstract：:The transition to a sustainable bio-based circular economy requires cutting edge technologies that ensure economic growth with environmentally responsible action. This transition will only be feasible when the opportunities of digitalization are also exploited. Digital methods and big data handling have already found ...

journal_title：New biotechnology

authors： Krüger A,Schäfers C,Busch P,Antranikian G

更新日期：2020-11-25 00:00:00

abstract：:The term 'liquid biopsy', introduced in 2013 in reference to the analysis of circulating tumour cells (CTCs) in cancer patients, was extended to cell-free nucleic acids (cfNAs) circulating in blood and other body fluids. CTCs and cfNAs are now considered diagnostic and prognostic markers, used as surrogate materials f...

journal_title：New biotechnology

authors： Salvianti F,Gelmini S,Costanza F,Mancini I,Sonnati G,Simi L,Pazzagli M,Pinzani P

更新日期：2020-03-25 00:00:00

abstract：:This work targets the valorization of brewer's spent grain (BSG) waste by ethanol production, providing strategies for increasing titers in the multiple process steps involved. High solid loadings and use of whole slurry from the pretreatment were evaluated, aiming to achieve high ethanol concentration and yield. As v...

journal_title：New biotechnology

authors： Pinheiro T,Coelho E,Romaní A,Domingues L

更新日期：2019-11-25 00:00:00

abstract：:CTX-M15 is one of the most widespread, extended spectrum β-lactamases, a major determinant of antibiotic resistance representing urgent public health threats, among enterobacterial strains infecting humans and animals. Here we describe the selection of binders to CTX-M15 from a combinatorial affibody library displayed...

journal_title：New biotechnology

authors： Lagoutte P,Lugari A,Elie C,Potisopon S,Donnat S,Mignon C,Mariano N,Troesch A,Werle B,Stadthagen G

更新日期：2019-05-25 00:00:00

abstract：:Polyhydroxyalkanoates (PHA) are microbial polyesters which accumulate as intracellular granules in numerous prokaryotes and mainly serve as storage materials; beyond this primary function, PHA also enhance the robustness of bacteria against various stress factors. We have observed that the presence of PHA in bacterial...

journal_title：New biotechnology

authors： Sedlacek P,Slaninova E,Koller M,Nebesarova J,Marova I,Krzyzanek V,Obruca S

更新日期：2019-03-25 00:00:00

abstract：:Feather hydrolysates were obtained through submerged cultivation of 50 g/L feathers with Chryseobacterium sp. kr6. Culture supernatants, displaying antioxidant properties, as evaluated by the 2,2'-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) radical scavenging method, were partially purified by gel-filtra...

journal_title：New biotechnology

authors： Fontoura R,Daroit DJ,Corrêa APF,Moresco KS,Santi L,Beys-da-Silva WO,Yates JR 3rd,Moreira JCF,Brandelli A

更新日期：2019-03-25 00:00:00

abstract：:Bread wheat (Triticum aestivum L.) is a staple food for a significant part of the world's population. The growing demand on its production can be satisfied by improving yield and resistance to biotic and abiotic stress. Knowledge of the genome sequence would aid in discovering genes and QTLs underlying these traits an...

journal_title：New biotechnology

authors： Tulpová Z,Luo MC,Toegelová H,Visendi P,Hayashi S,Vojta P,Paux E,Kilian A,Abrouk M,Bartoš J,Hajdúch M,Batley J,Edwards D,Doležel J,Šimková H

更新日期：2019-01-25 00:00:00

abstract：:This work has analysed the influence of CdCl2, NiCl2 and CuCl2 on simple phenolic acids, such as the 3PPP (phenylpyruvic, phenylacetic, and 4-hydroxyphenylpyruvic) and 2DR (3,4-dihydroxyphenylacetic and rosmarinic) phenolic acids for the first time and studied their interactions with antioxidant systems and the glutat...

journal_title：New biotechnology

authors： Strejckova A,Dvorak M,Klejdus B,Krystofova O,Hedbavny J,Adam V,Huska D

更新日期：2019-01-25 00:00:00

abstract：:The limited availability of metabolite-specific sensors for continuous sampling and monitoring is one of the main bottlenecks contributing to failures in bioprocess development. Furthermore, only a limited number of approaches exist to connect currently available measurement systems with high throughput reactor units....

journal_title：New biotechnology

authors： Fernandes AC,Semenova D,Panjan P,Sesay AM,Gernaey KV,Krühne U

更新日期：2018-12-25 00:00:00

abstract：:Although the application of microreactors in different processes has been extensively explored in recent decades, microreactors continue to be underexplored in the context of the enzyme-catalysed process for biodiesel production. Due to their numerous advantages, microreactors could become the next step in the develop...

journal_title：New biotechnology

authors： Šalić A,Tušek AJ,Sander A,Zelić B

更新日期：2018-12-25 00:00:00

abstract：:The development of a simple pH-stat fed-batch fermentation strategy for the production of Magnetospirillum gryphiswaldense MSR-1 and magnetosomes (nanoscale magnetic organelles with biotechnological applications) is described. Flow cytometry was exploited as a powerful analytical tool for process development, enabling...

journal_title：New biotechnology

authors： Fernández-Castané A,Li H,Thomas ORT,Overton TW

更新日期：2018-11-25 00:00:00

abstract：:A novel fermentation system was employed whereby the mycelial mat of Rhizopus oryzae was attached to a polypropylene tube. Batch operation was used for growth, while continuous operation was employed during the fumaric acid production phase. A clear decrease in respiration, fumaric acid (FA) and ethanol production was...

journal_title：New biotechnology

authors： Naude A,Nicol W

更新日期：2018-09-25 00:00:00

abstract：:The white rot fungus Pleurotus ostreatus HK 35, which is also an edible industrial mushroom commonly cultivated in farms, was tested in the degradation of typical representatives of endocrine disrupters (EDCs; bisphenol A, estrone, 17β-estradiol, estriol, 17α-ethinylestradiol, triclosan and 4-n-nonylphenol); its degra...

journal_title：New biotechnology

authors： Křesinová Z,Linhartová L,Filipová A,Ezechiáš M,Mašín P,Cajthaml T

更新日期：2018-07-25 00:00:00

abstract：:This work focused on the biodegradation of three structurally related fluoroacetates (FAs), mono- (MFA), di- (DFA) and trifluoroacetate (TFA), using as microbial inocula samples collected from a site with a long history of industrial contamination and activated sludge obtained from a municipal wastewater treatment pla...

journal_title：New biotechnology

authors： Alexandrino DAM,Ribeiro I,Pinto LM,Cambra R,Oliveira RS,Pereira F,Carvalho MF

更新日期：2018-07-25 00:00:00

abstract：:Tobacco Etch Virus (TEV) protease is used in the purification of recombinant proteins, but its usage is often hampered by solubility issues. Here, we report a short, 12-residue solubility enhancing peptide (SEP) tag attached at the C-terminus of TEV (TEV-C9R). We assessed the effects of the C9R tag on the biophysical ...

journal_title：New biotechnology

authors： Nautiyal K,Kuroda Y

更新日期：2018-05-25 00:00:00

abstract：:In the present study, different photoperiods and nutritional conditions were applied to a mixed wastewater-borne cyanobacterial culture in order to enhance the intracellular accumulation of polyhydroxybutyrates (PHBs) and carbohydrates. Two different experimental set-ups were used. In the first, the culture was perman...

journal_title：New biotechnology

authors： Arias DM,Uggetti E,García-Galán MJ,García J

更新日期：2018-05-25 00:00:00

abstract：:Human milk oligosaccharides (HMOs) constitute a unique family of bioactive lactose-based molecules present in human breast milk. HMOs are of major importance for infant health and development but also virtually absent from bovine milk used for infant formula. Among the HMOs, the fucosylated species are the most abunda...

journal_title：New biotechnology

authors： Zeuner B,Muschiol J,Holck J,Lezyk M,Gedde MR,Jers C,Mikkelsen JD,Meyer AS

更新日期：2018-03-25 00:00:00

abstract：:Feruloyl esterases (FAEs) are a diverse group of enzymes that specifically catalyze the hydrolysis of ester bonds between a hydroxycinnamic (e.g. ferulic) acid and plant poly- or oligosaccharides. FAEs as auxiliary enzymes significantly assist xylanolytic and pectinolytic enzymes in gaining access to their site of act...

journal_title：New biotechnology

authors： Dilokpimol A,Mäkelä MR,Varriale S,Zhou M,Cerullo G,Gidijala L,Hinkka H,Brás JLA,Jütten P,Piechot A,Verhaert R,Hildén KS,Faraco V,de Vries RP

更新日期：2018-03-25 00:00:00

abstract：:A bioactive O-carboxymethyl chitosan (CMCS) hydrogel crosslinked with natural phenolics with potential application in wound dressings was synthesized using a laccase from Myceliophthora thermophila (MTL). The highest degree of cross-linking (49.7%) was achieved with catechol. All the phenolic-CMCS hydrogels synthesize...

journal_title：New biotechnology

authors： Huber D,Grzelak A,Baumann M,Borth N,Schleining G,Nyanhongo GS,Guebitz GM

更新日期：2018-01-25 00:00:00

abstract：:To transform developing and least developing countries into industrialised ones, biotechnology could be deployed along the value chain, to provide support to the development of the bio-based industries in such a way to ensure sustainability of the sector and to reduce negative environmental impacts that might otherwis...

journal_title：New biotechnology

authors： Lokko Y,Heijde M,Schebesta K,Scholtès P,Van Montagu M,Giacca M

更新日期：2018-01-25 00:00:00

abstract：:Protein production using processed cell lysates is a core technology in synthetic biology and these systems are excellent to produce difficult toxins or membrane proteins. However, the composition of the central lysate of cell-free systems is still a "black box". Escherichia coli lysates are most productive for cell-f...

journal_title：New biotechnology

authors： Foshag D,Henrich E,Hiller E,Schäfer M,Kerger C,Burger-Kentischer A,Diaz-Moreno I,García-Mauriño SM,Dötsch V,Rupp S,Bernhard F

更新日期：2018-01-25 00:00:00

abstract：:Notch signalling is an evolutionarily highly conserved pathway that plays a crucial role during embryonic development and in tissue homeostasis maintenance during adult life. Abnormal Notch signalling has been implicated in several human genetic disorders and in multiple facets of cancer biology, including stem cell r...

journal_title：New biotechnology

authors： Lamy M,Ferreira A,Dias JS,Braga S,Silva G,Barbas A

更新日期：2017-10-25 00:00:00

abstract：:The potential of limited enzymatic poly(ethylene terephthalate) (PET) surface hydrolysis for the modification of track-etched (TE) membranes was investigated. Cutinases 1 and 2 from Thermobifida cellulosilytica as well as a fusion protein of cutinase 1 with the polymer binding module from the polyhydroxyalkanoate depo...

journal_title：New biotechnology

authors： Gamerith C,Gajda M,Ortner A,Acero EH,Guebitz GM,Ulbricht M

更新日期：2017-10-25 00:00:00

abstract：:The alcoholysis of triolein was used to explore the specific features of a recombinant Rhizopus oryzae lipase (rROL) for biodiesel synthesis. For this purpose, different acylglycerols were compared as substrates in lipase-catalysed transesterification. rROL was shown to exhibit a higher specificity towards 1-monoolein...

journal_title：New biotechnology

authors： Canet A,Benaiges MD,Valero F,Adlercreutz P

更新日期：2017-10-25 00:00:00

abstract：:Pseudomonas corrugata and P. mediterranea are soil inhabitant bacteria, generally living as endophytes on symptomless plants and bare soil, but also capable of causing plant diseases. They share a similar genome size and a high proteome similarity. P. corrugata produces many biomolecules which play an important role i...

journal_title：New biotechnology

authors： Licciardello G,Ferraro R,Russo M,Strozzi F,Catara AF,Bella P,Catara V

更新日期：2017-07-25 00:00:00

abstract：:A pure culture of the filamentous bacterium Thiothrix, strain CT3, was aerobically cultured in a chemostat under continuous acetate feeding at three different culture residence times (RT 6, 12 or 22 d) and the same volumetric organic load rate (OLR 0.12gCOD/L/d). Cells cultured at decreasing RT in the chemostat had an...

journal_title：New biotechnology

authors： Valentino F,Beccari M,Villano M,Tandoi V,Majone M

更新日期：2017-05-25 00:00:00

abstract：:Understanding the capacity of Paenibacillus polymyxa DSM 365 to tolerate increasing concentrations of 2,3-butanediol (2,3-BD) is critical to engineering a 2,3-BD-overproducing strain. Hence, we investigated the response of P. polymyxa to high 2,3-BD concentrations. In fed-batch cultures (6-L bioreactor) 2,3-BD was acc...

journal_title：New biotechnology

authors： Okonkwo CC,Ujor V,Ezeji TC

更新日期：2017-01-25 00:00:00

abstract：:Cancer biomarkers represent a revolutionary advance toward personalised cancer treatment, promising therapies that are tailored to subgroups of patients sharing similar generic traits. Notwithstanding the optimism driving this development, biomarkers also present an array of social and ethical questions, as witnessed ...

journal_title：New biotechnology

authors： Blanchard A

更新日期：2016-12-25 00:00:00

abstract：:Microorganisms from extreme and restrictive eco systems, such as the Antarctic continent, are of great interest due to their ability to synthesize products of commercial value. Among these, enzymes from psychrotolerant and psychrophilic microorganisms offer potential economical benefits due to their high activity at l...

journal_title：New biotechnology

authors： Chaud LC,Lario LD,Bonugli-Santos RC,Sette LD,Pessoa Junior A,Felipe MD

更新日期：2016-12-25 00:00:00

abstract：:Cytokinins represent an important group of plant growth regulators that can modulate several biotechnological processes owing to their ability to influence almost all stages of plant development and growth. In addition, the use of purine based cytokinins with aromatic substituent in C6 position of the purine moiety in...

journal_title：New biotechnology

authors： Plíhalová L,Vylíčilová H,Doležal K,Zahajská L,Zatloukal M,Strnad M

更新日期：2016-09-25 00:00:00

abstract：:Rhodococcus fascians is a phytopathogenic Gram-positive Actinomycete with a very broad host range encompassing especially dicotyledonous herbaceous perennials, but also some monocots, such as the Liliaceae and, recently, the woody crop pistachio. The pathogenicity of R. fascians strain D188 is known to be encoded by t...

journal_title：New biotechnology

authors： Francis IM,Stes E,Zhang Y,Rangel D,Audenaert K,Vereecke D

更新日期：2016-09-25 00:00:00

abstract：:Marine organisms constitute approximately one-half of the total global biodiversity, being rich reservoirs of structurally diverse biofunctional components. The potential of cyanobacteria, micro- and macroalgae as sources of antimicrobial, antitumoral, anti-inflammatory, and anticoagulant compounds has been reported e...

journal_title：New biotechnology

authors： Montalvão S,Demirel Z,Devi P,Lombardi V,Hongisto V,Perälä M,Hattara J,Imamoglu E,Tilvi SS,Turan G,Dalay MC,Tammela P

更新日期：2016-05-25 00:00:00

abstract：:Zero-valent iron nanoparticles (nZVI) are a relatively new option for the treatment of contaminated soil and groundwater. However, because of their apparent toxicity, nZVI in high concentrations are known to interfere with many autochthonous microorganisms and, thus, impact their participation in the remediation proce...

journal_title：New biotechnology

authors： Pádrová K,Maťátková O,Šiková M,Füzik T,Masák J,Čejková A,Jirků V

更新日期：2016-01-25 00:00:00

abstract：:The use of mixed microbial cultures for the production of polyhydroxyalkanoates (PHAs) is emerging as a viable technology. In this study, 16S rRNA gene amplicon pyrosequencing was used to analyse fluctuations in populations over a 63-day period within a PHA-storing mixed microbial community enriched on fermented whey ...

journal_title：New biotechnology

authors： Janarthanan OM,Laycock B,Montano-Herrera L,Lu Y,Arcos-Hernandez MV,Werker A,Pratt S

更新日期：2016-01-25 00:00:00

abstract：:Rhamnogalacturonan I (RGI) modifying enzymes catalyse the degradation of the RGI backbone and encompass enzymes specific for either the α1,2-bond linking galacturonic acid to rhamnose or the α1,4-bond linking rhamnose to galacturonic acid in the RGI backbone. The first microbial enzyme found to be able to catalyse the...

journal_title：New biotechnology

authors： Silva IR,Jers C,Meyer AS,Mikkelsen JD

更新日期：2016-01-25 00:00:00