Abstract:
:To investigate the importance of the seventh residue of the second and third repeat fragments (R2 and R3 peptides) of the microtubule-binding domain (MBD) for tau filamentous assembly, the residues Lys and Pro were substituted (R2-K7P and R3-P7K). The filament formations of the R2 and R3 peptides were almost lost due to their substitutions despite their overall conformational similarities. The NOE analyses showed the importance of the conformational flexibility for the R2 peptide and the coupled extended and helical conformations for the R3 peptide in their limited N-terminal regions around their seventh residues. The result shows that the filament formation of MBD is initiated from a short fragment region containing the minimal conformational or functional motif.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tokimasa M,Minoura K,Hiraoka S,Tomoo K,Sumida M,Taniguchi T,Ishida Tdoi
10.1016/j.febslet.2005.05.020keywords:
subject
Has Abstractpub_date
2005-07-04 00:00:00pages
3481-6issue
17eissn
0014-5793issn
1873-3468pii
S0014-5793(05)00619-8journal_volume
579pub_type
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