The protease core of the muscle-specific calpain, p94, undergoes Ca2+-dependent intramolecular autolysis.

abstract：:MPD1, a yeast gene the overexpression of which suppresses the inviability caused by the loss of protein disulfide isomerase (PDI) was isolated and characterized. The MPD1 gene product retained a single disulfide isomerase active site sequence (APWCGHCK), an N-terminal putative signal sequence, and a C-terminal endopla...

journal_title：FEBS letters

authors： Tachikawa H,Takeuchi Y,Funahashi W,Miura T,Gao XD,Fujimoto D,Mizunaga T,Onodera K

更新日期：1995-08-07 00:00:00

abstract：:The state of assembly of the pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes was examined after the dihydrolipoyl acyltransferase (E2) component of each enzyme system had been subjected to varying degrees of limited proteolysis. Dissociation of the dihydrolipoyl dehydrogenase (E3) component accompanied...

journal_title：FEBS letters

authors： Packman LC,Perham RN

更新日期：1986-10-06 00:00:00

abstract：:Silent information regulator 2 (Sir2) is an NAD(+)-dependent histone deacetylase that establishes repressive chromatin status and extends the life span of both budding yeast and the nematode worm Caenorhabditis elegans. There is growing evidence that its mammalian homologue Sir2alpha protects cells from stress-induced...

journal_title：FEBS letters

authors： Ohsawa S,Miura M

更新日期：2006-10-30 00:00:00

abstract：:The F-box protein Skp2 is important for S phase entry and binds to Skp1 and the cyclin A-Cdk2 complex. Here we report the cloning, analysis of genomic organization and characterization of a novel gene product related to Skp2 named FBL2. The human FBL2 gene was found to be a highly interrupted gene of at least 126.6 kb...

journal_title：FEBS letters

authors： Ilyin GP,Rialland M,Glaise D,Guguen-Guillouzo C

更新日期：1999-10-01 00:00:00

abstract：:In this work, we examined the antioxidant role of mucin, a typical sialic acid containing high-molecular weight glycoprotein. The function of mucin as a hydroxyl radical (.OH) scavenger was characterized using bovine submaxillary gland mucin (BSM). Non-treated BSM effectively protected DNA from the attack of .OH; howe...

journal_title：FEBS letters

authors： Ogasawara Y,Namai T,Yoshino F,Lee MC,Ishii K

更新日期：2007-05-29 00:00:00

abstract：:The degradation of the erythro and threo isomers of the non-phenolic lignin model compound 2-(2,6-dimethoxyphenoxy)-1-(3,4-dimethoxyphenyl)-1,3-propanediol was examined. Enzymic and non-enzymic oxidation of the diastereomers was performed with Trametes versicolor lignin peroxidase and cerium(IV) ammonium nitrate, resp...

journal_title：FEBS letters

authors： Jönsson L,Karlsson O,Lundquist K,Nyman PO

更新日期：1990-12-10 00:00:00

abstract：:Somatostatin 14 (SS 14) has been isolated from pancreatic extracts, but open gel filtration immunoreactive SS often elutes in two peaks. We isolated both peaks, but upon sequence analysis only authentic SS 14 could be identified. By further gel filtration experiments it turned out that both synthetic and extractable S...

journal_title：FEBS letters

authors： Bersani M,Johnsen AH,Holst JJ

更新日期：1991-02-25 00:00:00

abstract：:We examined the effect of sodium butyrate on in vitro angiogenesis and cyclooxygenase (COX) expression using primary cultures of human intestinal microvascular endothelial cells (HIMEC). Butyrate inhibited VEGF-induced cellular proliferation, transmigration and tube formation of HIMEC. Butyrate also inhibited COX-2 ex...

journal_title：FEBS letters

authors： Ogawa H,Rafiee P,Fisher PJ,Johnson NA,Otterson MF,Binion DG

更新日期：2003-11-06 00:00:00

abstract：:Expression from a plasmid of the complete gene, including the codons for the N-terminal periplasmic targeting signal, for cytochrome c550 of Paracoccus denitrificans led to the formation of the holo protein in the periplasms of both P. denitrificans and Escherichia coli. Expression of the gene from which the region co...

journal_title：FEBS letters

authors： Sambongi Y,Ferguson SJ

更新日期：1994-02-28 00:00:00

abstract：:CAP (c-Cbl associated protein)/ponsin belongs to a family of adaptor proteins implicated in cell adhesion and signaling. Here we show that CAP binds to and co-localizes with the essential endocytic factor dynamin. We demonstrate that CAP promotes the formation of dynamin-decorated tubule like structures, which are als...

journal_title：FEBS letters

authors： Tosoni D,Cestra G

更新日期：2009-01-22 00:00:00

abstract：:1H NMR spectra of skinned rabbit muscle fibers show a group of relatively sharp resonance lines which presumably originate from highly mobile protein domains. Comparison with the spectrum of myosin subfragment 1 suggests that these signals may come at least partly from mobile regions of the myosin head. NMR could poss...

journal_title：FEBS letters

authors： Kalbitzer HR,Schrumpf M,Wray J

更新日期：1992-02-24 00:00:00

abstract：:Classically, alpha-1,4-glucan synthases have been divided into two families, animal/fungal glycogen synthases (GS) and bacterial/plant starch synthases (G(S)S), according to differences in sequence, sugar donor specificity and regulatory mechanisms. Detailed sequence analysis, predicted secondary structure comparison ...

journal_title：FEBS letters

authors： Cid E,Geremia RA,Guinovart JJ,Ferrer JC

更新日期：2002-09-25 00:00:00

abstract：:A recombinant expression system was developed to analyse sequence determinants involved in O-glycosylation of proteins in mycobacteria. By expressing peptide sequences corresponding to known glycosylation sites within a chimeric lipoprotein construct, amino acids flanking modified threonine residues were found to have...

journal_title：FEBS letters

authors： Herrmann JL,Delahay R,Gallagher A,Robertson B,Young D

更新日期：2000-05-19 00:00:00

abstract：:The effects of synthetic rat adrenomedullin (rAM), a novel vasorelaxant peptide originally isolated from human pheochromocytoma, on receptor binding and cAMP generation were studied in cultured rat vascular smooth muscle cells (VSMC). A binding study using [125I]rAM revealed the presence of a single class of high-affi...

journal_title：FEBS letters

authors： Eguchi S,Hirata Y,Kano H,Sato K,Watanabe Y,Watanabe TX,Nakajima K,Sakakibara S,Marumo F

更新日期：1994-03-07 00:00:00

abstract：:Extraction and identification of the non-covalently bound heme groups from crude membrane preparations of photoheterotrophically grown Synechocystis sp. PCC 6803 by reversed phase high performance liquid chromatography and optical spectrophotometry led to the detection of heme O in addition to hemes B and A which latt...

journal_title：FEBS letters

authors： Peschek GA,Wastyn M,Fromwald S,Mayer B

更新日期：1995-09-04 00:00:00

abstract：:The carbohydrate binding properties of jacalin lectin were examined using RAF9 cell-derived D-[6-3H]glucosamine-radiolabeled total glycopeptides containing N-linked and O-linked oligosaccharides. The binding of N-linked glycopeptides to jacalin was abolished by treatment of alpha-galactosidase whereas O-linked glycope...

journal_title：FEBS letters

authors： Do SI,Lee KY

更新日期：1998-01-09 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae the genes involved in galactose metabolism (GAL1,7,10) are transcriptionally activated more than a 1000-fold in the presence of galactose as the sole carbon source in the culture media. In the present work, we monitored the activity of the GAL10 gene promoter in different Ras-cAMP...

journal_title：FEBS letters

authors： Mirisola MG,Gallo A,De Leo G

更新日期：2007-05-15 00:00:00

abstract：:The structural basis of GABA-gated chloride channels in mammalian brain is presently explored by the functional expression of cDNAs coding for the alpha, beta or gamma-subunits of the receptor and their isoforms. In this context, we expressed the cloned cDNA coding for the rat beta 1-subunit of the GABAA receptor in t...

journal_title：FEBS letters

authors： Sigel E,Baur R,Malherbe P,Möhler H

更新日期：1989-11-06 00:00:00

abstract：:Here, we investigated the molecular mechanism underlying the changes in the distribution of nucleolin. Our study identified PI3K/Akt signaling as an essential pathway regulating the distribution of nucleolin. Furthermore, nucleolin can interact with phospho-PI3K-p55, and changes in the distribution of nucleolin were r...

journal_title：FEBS letters

authors： Wu DM,Zhang P,Liu RY,Sang YX,Zhou C,Xu GC,Yang JL,Tong AP,Wang CT

更新日期：2014-05-21 00:00:00

abstract：:Glycine amide (GlyAd), a typically amidated amino acid, is a versatile additive that suppresses protein aggregation during refolding, heat treatment, and crystallization. In spite of its effectiveness, the exact mechanism by which GlyAd suppresses protein aggregation remains to be elucidated. Here, we show the crystal...

journal_title：FEBS letters

authors： Ito L,Shiraki K,Makino M,Hasegawa K,Kumasaka T

更新日期：2011-02-04 00:00:00

abstract：:Human aminopeptidase N carries an apical sorting signal on its ectodomain necessary for its correct transport to the apical membrane in Madin-Darby canine kidney cells. To determine whether the apical sorting signal is localized in the serine/threonine rich stalk or in the catalytic head group, anchor/stalk-minus amin...

journal_title：FEBS letters

authors： Vogel LK,Norén O,Sjöström H

更新日期：1992-08-10 00:00:00

abstract：:Polyglutamine tract-binding protein 1 (PQBP1) is an intrinsically disordered protein abundantly expressed in the brain. Mutations in the PQBP1 gene are causative for X-linked mental retardation disorders. Here, we investigated the structure of the C-terminal segment within the context of full-length PQBP1. We produced...

journal_title：FEBS letters

authors： Nabeshima Y,Mizuguchi M,Kajiyama A,Okazawa H

更新日期：2014-12-20 00:00:00

abstract：:Hell's Gate globin I (HGbI), a heme-containing protein structurally homologous to mammalian neuroglobins, has been identified from an acidophilic and thermophilic obligate methanotroph, Methylacidiphilum infernorum. HGbI has very high affinity for O(2) and shows barely detectable autoxidation in the pH range of 5.2-8....

journal_title：FEBS letters

authors： Teh AH,Saito JA,Baharuddin A,Tuckerman JR,Newhouse JS,Kanbe M,Newhouse EI,Rahim RA,Favier F,Didierjean C,Sousa EH,Stott MB,Dunfield PF,Gonzalez G,Gilles-Gonzalez MA,Najimudin N,Alam M

更新日期：2011-10-20 00:00:00

abstract：:Capsaicin, a major ingredient of hot pepper, is considered to exhibit anti-inflammatory properties. Our previous study demonstrated that capsaicin inhibited the production of pro-inflammatory mediators through NF-kappaB inactivation in LPS-stimulated macrophages. In order to further clarify the mechanism underlying th...

journal_title：FEBS letters

authors： Park JY,Kawada T,Han IS,Kim BS,Goto T,Takahashi N,Fushiki T,Kurata T,Yu R

更新日期：2004-08-13 00:00:00

abstract：:We identified a gene encoding a novel secreted protein in mice, humans, and zebrafish. As the protein of 222 amino acids is similar to Brorin, a secreted BMP antagonist, which is a member of the Chordin family, we named it Brorin-like. Recombinant Brorin-like protein weakly but significantly inhibited the activity of ...

journal_title：FEBS letters

authors： Miwa H,Miyake A,Kouta Y,Shimada A,Yamashita Y,Nakayama Y,Yamauchi H,Konishi M,Itoh N

更新日期：2009-11-19 00:00:00

abstract：:Gamma-aminobutyric acid type A (GABAA) receptor beta1 (gabrb1), a subunit of GABAA receptors involved in inhibitory effects on neurotransmission, was found to associate with the formation of protease-resistant prion protein in prion-infected neuroblastoma cells. Silencing of gabrb1 gene expression significantly decrea...

journal_title：FEBS letters

authors： Kimura T,Ishikawa K,Sakasegawa Y,Teruya K,Sata T,Schätzl H,Doh-ura K

更新日期：2010-03-19 00:00:00

abstract：:Physiological and pathological states that are associated with elevated plasma fatty acids (FAs) increase uncoupling protein 2 (UCP2) mRNA in white adipose tissue and UCP3 mRNA in skeletal muscle and heart. A direct effect of unsaturated fatty acids from all classes has been shown in various cultured cells. There is e...

journal_title：FEBS letters

authors： Thompson MP,Kim D

更新日期：2004-06-18 00:00:00

abstract：:Perturbation of the aerobic steady-state in a chemostat culture of the ethanol-producing bacterium Zymomonas mobilis with a small pulse of ethanol causes a burst of ethanol oxidation, although the reactant ratio of the alcohol dehydrogenase (ADH) reaction ([NADH][acetaldehyde][H(+)])/([ethanol][NAD(+)]) remains above ...

journal_title：FEBS letters

authors： Kalnenieks U,Galinina N,Toma MM,Pickford JL,Rutkis R,Poole RK

更新日期：2006-09-18 00:00:00

abstract：:In the lysosomal storage disorder Gaucher disease (GD), glucosylceramide (GlcCer) accumulates due to the defective activity of glucocerebrosidase. A subset of GD patients develops neuropathology. We now show mislocalization of Limp2-positive puncta and a large reduction in the number of Lamp1-positive puncta, which ar...

journal_title：FEBS letters

authors： Zigdon H,Meshcheriakova A,Farfel-Becker T,Volpert G,Sabanay H,Futerman AH

更新日期：2017-03-01 00:00:00

abstract：:Apoptosis repressor with caspase recruitment domain is expressed at high levels in brain and myogenic tissues, consistent with a role to inhibit apoptosis in the terminally differentiated cells. Expression of ARC in cancers is not known. In this study, we reported that ARC was highly expressed in various non-myogenic ...

journal_title：FEBS letters

authors： Wang M,Qanungo S,Crow MT,Watanabe M,Nieminen AL

更新日期：2005-04-25 00:00:00