Isolation and characterization of a yeast gene, MPD1, the overexpression of which suppresses inviability caused by protein disulfide isomerase depletion.

abstract：:The dynamic properties of 111 backbone HN sites in uncomplexed human profilin, a protein of 139 residues, have been characterized by two-dimensional inverse-detected 1H-15N NMR spectroscopy. Heteronuclear (1H)-15N nuclear Overhauser effects and 15N longitudinal and transverse relaxation rates have been analyzed in ter...

journal_title：FEBS letters

authors： Constantine KL,Friedrichs MS,Bell AJ,Lavoie TB,Mueller L,Metzler WJ

更新日期：1993-12-28 00:00:00

abstract：:It is increasingly clear that microRNAs play a crucial role in tumorigenesis. Recently, emerging evidence suggested that miR-26a is aberrantly expressed in tumor tissues. In our study, frequent down-regulation of miR-26a was observed in 10 human bladder cancer tissues. Forced expression of miR-26a in the bladder cance...

journal_title：FEBS letters

authors： Lin Y,Chen H,Hu Z,Mao Y,Xu X,Zhu Y,Xu X,Wu J,Li S,Mao Q,Zheng X,Xie L

更新日期：2013-08-02 00:00:00

abstract：:The beta-adrenergic receptor kinase mediates agonist-dependent phosphorylation of beta-adrenergic receptors, which is thought to represent the first step of homologous desensitization. We have expressed bovine and human beta ARK1 in Sf9 cells and purified them to apparent homogeneity in milligram quantities. The Km-va...

journal_title：FEBS letters

authors： Söhlemann P,Hekman M,Buchen C,Elce JS,Lohse MJ

更新日期：1993-06-07 00:00:00

abstract：:Several types of post-translational modifications contribute to the high level of tubulin heterogeneity in the brain. An important modification is glutamylation of the major brain-specific isotypes, such as class Ia/b of alpha-tubulin and classes II and III of beta-tubulin. Here we describe experiments to determine if...

journal_title：FEBS letters

authors： Mary J,Redeker V,Le Caer JP,Promé JC,Rossier J

更新日期：1994-10-10 00:00:00

abstract：:There are numerous examples of how fundamental research has been required to understand and treat human disease. This article focuses on three human diseases of lipid metabolism in which advancements in understanding and treatment would not have been possible without basic research. Fabry disease is an inherited metab...

journal_title：FEBS letters

authors： Vance DE

更新日期：2006-10-09 00:00:00

abstract：:The tetracycline-controlled transcription system has become one of the most potent systems for experimental manipulations of transcription levels in vivo. Here we report on rtTA variants, which were generated by combining the existing positively regulated Tet repressor domains of rtTA and rtTA-M2 with a modified and m...

journal_title：FEBS letters

authors： Kämper MR,Gohla G,Schlüter G

更新日期：2002-04-24 00:00:00

abstract：:The 77 kDa subunit of the polyadenylation cleavage stimulation factor (CstF77) is important in messenger RNA 3' end processing. Previously, we demonstrated that AtCstF77 interacts with AtCPSF30, the Arabidopsis ortholog of the 30 kDa subunit of the Cleavage and Polyadenylation Specificity Factor. In further dissecting...

journal_title：FEBS letters

authors： Bell SA,Hunt AG

更新日期：2010-04-16 00:00:00

abstract：:Structural analogs of adenosine 5'-acylphosphates, which are intermediates of the reaction catalyzed by acyl-CoA synthetases, were synthesized by condensing primary alcohols with AMP to examine the inhibitory effects on the lignoceroyl-CoA and palmitoyl-CoA synthetase activities. Hexadecyl, octadecyl, eicosyl, docosyl...

journal_title：FEBS letters

authors： Shiraishi T,Tezuka K,Uda Y

更新日期：1994-10-03 00:00:00

abstract：:Crystallographic analysis of a mutated form of "loopful" GH19 chitinase from rye seeds a double mutant RSC-c, in which Glu67 and Trp72 are mutated to glutamine and alanine, respectively, (RSC-c-E67Q/W72A) in complex with chitin tetrasaccharide (GlcNAc)₄ revealed that the entire substrate-binding cleft was completely o...

journal_title：FEBS letters

authors： Ohnuma T,Umemoto N,Kondo K,Numata T,Fukamizo T

更新日期：2013-08-19 00:00:00

abstract：:Nitrogen dioxide (NO2.) is often present in inhaled air and may be generated in vivo from nitric oxide. Exposure of human blood plasma to NO2. caused rapid losses of ascorbic acid, uric acid and protein thiol groups, as well as lipid peroxidation and depletions of alpha-tocopherol, bilirubin and ubiquinol-10. No incre...

journal_title：FEBS letters

authors： Halliwell B,Hu ML,Louie S,Duvall TR,Tarkington BK,Motchnik P,Cross CE

更新日期：1992-11-16 00:00:00

abstract：:Two human mammary carcinoma cell variants, MCF-7/AZ and MCF-7/6, show the same composition in their glycosphingolipid-enriched microdomain (GEM) with regard to globo-series structures Gb3, Gb4, Gb5, monosialyl-Gb5, GM2, and cSrc and FAK. Both variants are non-invasive into collagen gel layer, and showed similar motili...

journal_title：FEBS letters

authors： Steelant WF,Kawakami Y,Ito A,Handa K,Bruyneel EA,Mareel M,Hakomori S

更新日期：2002-10-30 00:00:00

abstract：:Protein tyrosine kinase p72syk purified from rat spleen has been assayed for its ability to phosphorylate a number of peptide substrates derived from naturally occurring phospho-acceptor sites. The phosphorylation efficiency is extremely variable, depending on the peptide sequence, with Km values in the 3-1500 microM ...

journal_title：FEBS letters

authors： Brunati AM,Donella-Deana A,Ruzzene M,Marin O,Pinna LA

更新日期：1995-06-26 00:00:00

abstract：:We show that N-1 in adenine of chromosomal DNA is methylated by treatment of metaphase chromosomes with dimethylsulphate while this is not the case in chromatin. The data on methylation are consistent with those obtained from the experiments with S1-nuclease treatment of chromatin and chromosomes. This suggests a disa...

journal_title：FEBS letters

authors： Belyaev ND,Budker VG,Dubrovskaya VA,Matveeva NM

更新日期：1983-04-18 00:00:00

abstract：:The dependence of CMP-N-acetylneuraminate:GM1 sialyltransferase (SAT IV) activity of rat liver Golgi apparatus on GM1 ganglioside ceramide composition was evaluated. SAT IV activity was assayed on GM1 molecular species carrying homogeneous ceramide moieties containing long chain bases of different length (18 or 20 C a...

journal_title：FEBS letters

authors： Pitto M,Palestini P,Masserini M

更新日期：1996-04-01 00:00:00

abstract：:Evidence is presented, by means of both fluorescence and action spectroscopy, that a small, spectroscopically heterogeneous population of both Chl a and Chl b molecules is present in isolated spinach thylakoids and is active in photoinhibition. The broadness of the action spectrum suggests that degraded or incompletel...

journal_title：FEBS letters

authors： Santabarbara S,Neverov KV,Garlaschi FM,Zucchelli G,Jennings RC

更新日期：2001-02-23 00:00:00

abstract：:We report the cDNA-deduced primary structure of the chicken counterpart of the murine dilute gene product, a member of the myosin I family. Comparison of the chicken and mouse sequences reveals a distinct pattern of domains of high and low sequence conservation. An internal deletion of 25 amino acids probably reflects...

journal_title：FEBS letters

authors： Sanders G,Lichte B,Meyer HE,Kilimann MW

更新日期：1992-10-26 00:00:00

abstract：:We have purified two growth factors from dogfish brain and retina by using their binding affinity for heparin-Sepharose. These growth factors were eluted at 1 and 2 M NaCl similarly to those purified from bovine brain or retina. Their mitogenic activity was assayed in vitro on the same mammalian cells: bovine lens epi...

journal_title：FEBS letters

authors： Lagente O,Diry M,Courtois Y

更新日期：1986-07-07 00:00:00

abstract：:Alternative splicing of tau exon 10 generates tau isoforms with three or four microtubule-binding repeats, named 3R- or 4R-tau. Normal adult human brain expresses equal levels of them. Imbalance of 3R-tau and 4R-tau associates with several tauopathies. Splicing factor 9G8 suppresses tau exon 10 inclusion and its funct...

journal_title：FEBS letters

authors： Gu J,Shi J,Wu S,Jin N,Qian W,Zhou J,Iqbal IG,Iqbal K,Gong CX,Liu F

更新日期：2012-07-30 00:00:00

abstract：:Phosphorylation of fibronectin was studied in quiescent vs growing cells from several species. Fibronectin secreted by actively growing cells exhibits a significantly higher level of phosphorylation than does the fibronectin secreted by quiescent cells of the same species. ...

journal_title：FEBS letters

authors： Ali IU

更新日期：1983-01-10 00:00:00

abstract：:Treatment of cultured carrot cells with dibutyryl cAMP or forskolin resulted in the appreciable decrease in extracellular K+ concentration. This decrease was found to be transient and the concentration of the ion in the culture medium restored to the original level within few minutes. The cAMP-induced decrease in K+ l...

journal_title：FEBS letters

authors： Kurosaki F

更新日期：1997-05-12 00:00:00

abstract：:Three DNA restriction fragments designated tyrT, 102-mer and 70-mer, have been used as substrates for footprinting studies using DNase I in the presence of the structurally similar antibiotics chartreusin and elsamicin A. The sequence-selective binding sites of the antibiotics can be mapped in regions which are rich i...

journal_title：FEBS letters

authors： Salas X,Portugal J

更新日期：1991-11-04 00:00:00

abstract：:Amino acid residues 3-15 of mycobacterial HSP60 define a dominant T-cell epitope for HLA-DR3+ve humans and Mamu-DR3+ve rhesus monkeys. Our results show that Mamu-DR3 molecules on PBMC can be efficiently loaded in vivo with the above-mentioned peptides when they are intravenously injected encapsulated in liposomes, but...

journal_title：FEBS letters

authors： t Hart BA,Elferink DG,Drijfhout JW,Storm G,van Blooijs L,Bontrop RE,de Vries RR

更新日期：1997-06-02 00:00:00

abstract：:The influence of poly (ADP-ribose) polymerase (PARP) and poly ADP-ribosylation on DNA synthesis supported by human replicative DNA polymerase (DNA pol) alpha, delta, and epsilon has been examined using the replication system containing poly(dA)4500-oligo(dT)12-18 as the template primer. PARP alone inhibited the pol ac...

journal_title：FEBS letters

authors： Eki T

更新日期：1994-12-19 00:00:00

abstract：:Streptozotocin (STZ)-induced diabetic animals are vulnerable to cold stress. Uncoupling proteins (UCPs) play an important role in regulating thermogenesis. We investigated the gene expressions of UCPs in brown adipose tissue (BAT), white adipose tissue (WAT), liver and gastrocnemius muscle of STZ-diabetic rats using N...

journal_title：FEBS letters

authors： Kageyama H,Suga A,Kashiba M,Oka J,Osaka T,Kashiwa T,Hirano T,Nemoto K,Namba Y,Ricquier D,Giacobino JP,Inoue S

更新日期：1998-12-04 00:00:00

abstract：:AQP3 is a water/glycerol transporter expressed at the basolateral membrane of colonic epithelial cells. Although AQPs are expressed in the gastrointestinal tract, their effect on intestinal barrier has not been clear. Here, we showed that knockdown of AQP3 caused a dramatic, dose-dependent increase in E. coli C25 tran...

journal_title：FEBS letters

authors： Zhang W,Xu Y,Chen Z,Xu Z,Xu H

更新日期：2011-10-03 00:00:00

abstract：:The addition of 5-azacytidine to erythroleukemic cells which were induced to differentiate with DMSO or BA altered the expression of the hemoglobins. After the addition of 5-azacytidine there was an increase in hemoglobin synthesis especially in the embryonic E2 band. The beta-globin increased in synthesis after 5-aza...

journal_title：FEBS letters

authors： Zucker RM,Decal DL,Whittington KB

更新日期：1983-10-17 00:00:00

abstract：:This paper reviews our present knowledge of sphingomyelinases as enzymes, and as enzymes acting on a membrane constituent lipid, sphingomyelin. Six types of sphingomyelinases are considered, namely acidic, secretory, Mg(2+)-dependent neutral, Mg(2+)-independent neutral, alkaline, and bacterial enzymes with both phosph...

journal_title：FEBS letters

authors： Goñi FM,Alonso A

更新日期：2002-10-30 00:00:00

abstract：:A latent MAP kinase kinase kinase activity previously detected after chromatography of PC12 cell extracts on Mono Q [(1992) FEBS Lett. 314, 461-465] has been identified as B-Raf by immunoblotting and immunoprecipitation experiments and by its specific activation with B-Raf antibodies. B-Raf is phosphorylated after sti...

journal_title：FEBS letters

authors： Traverse S,Cohen P

更新日期：1994-08-15 00:00:00

abstract：:To investigate the importance of the seventh residue of the second and third repeat fragments (R2 and R3 peptides) of the microtubule-binding domain (MBD) for tau filamentous assembly, the residues Lys and Pro were substituted (R2-K7P and R3-P7K). The filament formations of the R2 and R3 peptides were almost lost due ...

journal_title：FEBS letters

authors： Tokimasa M,Minoura K,Hiraoka S,Tomoo K,Sumida M,Taniguchi T,Ishida T

更新日期：2005-07-04 00:00:00

abstract：:cDNA clones encoding the 45 kDa protein were isolated from a rat olfactory epithelium cDNA library and their inserts were sequenced. The reconstructed protein sequence comprises 400 amino acids with a calculated molecular mass of 46,026 Da. A homology was revealed between the amino acid sequence of the 45 kDa protein ...

journal_title：FEBS letters

authors： Merkulova MI,Andreeva SG,Shuvaeva TM,Novoselov SV,Peshenko IV,Bystrova MF,Novoselov VI,Fesenko EE,Lipkin VM

更新日期：1999-04-30 00:00:00