Abstract:
:The beta-adrenergic receptor kinase mediates agonist-dependent phosphorylation of beta-adrenergic receptors, which is thought to represent the first step of homologous desensitization. We have expressed bovine and human beta ARK1 in Sf9 cells and purified them to apparent homogeneity in milligram quantities. The Km-values of the enzyme were 3.8 microM for rhodopsin and 22 microM for ATP; the Vmax-value was 9.9 mol phosphate/mol beta ARK/min. These data indicate that the two recombinant kinases were at least as active as preparations previously obtained from bovine brain. There were no differences in the functional activity of human and bovine beta ARK.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Söhlemann P,Hekman M,Buchen C,Elce JS,Lohse MJdoi
10.1016/0014-5793(93)81532-5subject
Has Abstractpub_date
1993-06-07 00:00:00pages
59-62issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(93)81532-5journal_volume
324pub_type
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