Abstract:
:In yeast, three AAA superfamily metalloproteases (Yme1p, Afg3p and Rca1p) are localized to the mitochondrial inner membrane where they perform roles in the assembly and turnover of the respiratory chain complexes. We have investigated the function of the proposed human orthologue of yeast Yme1p, encoded by the YME1L gene on chromosome 10p. Transfection of both HEK-293EBNA and yeast cells with a green fluorescent protein-tagged YME1L cDNA confirmed mitochondrial targeting. When expressed in a yme1 disruptant yeast strain, YME1L restored growth on glycerol at 37 degrees C. We propose that YME1L plays a phylogenetically conserved role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Shah ZH,Hakkaart GA,Arku B,de Jong L,van der Spek H,Grivell LA,Jacobs HTdoi
10.1016/s0014-5793(00)01859-7keywords:
subject
Has Abstractpub_date
2000-08-04 00:00:00pages
267-70issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01859-7journal_volume
478pub_type
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