Abstract:
:The promoter of the cholecystokinin (CCK) gene possesses evolutionary conserved juxtaposed E-box and cAMP/TPA responsive elements (CRE/TRE). We have examined the functional interaction of these two sites. As previously noted, c-Jun/c-Fos heterodimers greatly increase promoter activity through association with the CRE/TRE. Mutation of the E-box enhanced the activation by c-Jun/c-Fos, as well as stimulation by forskolin and bFGF, that acts through the CRE/TRE site. Moreover, c-Jun/c-Fos stimulation was inhibited by co-expression of c-Myc and Max. The results indicate that factors associating with the E-box exhibit a negative cooperative effect on the activation via the CRE/TRE element. We propose that this mechanism plays a significant role in CCK gene transcription and other genes with juxtaposed E-box and CRE/TRE.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Rourke IJ,Hansen TV,Nerlov C,Rehfeld JF,Nielsen FCdoi
10.1016/s0014-5793(99)00320-8keywords:
subject
Has Abstractpub_date
1999-04-01 00:00:00pages
15-8issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00320-8journal_volume
448pub_type
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